Xiaopeng Bai, Giada De Palma, Elisa Boschetti, Yuichiro Nishihara, Jun Lu, Chiko Shimbori, Anna Costanzini, Zarwa Saqib, Narjis Kraimi, Sacha Sidani, Siegfried Hapfelmeier, Andrew J Macpherson, Elena F Verdu, Roberto De Giorgio, Stephen M Collins, Premysl Bercik
BACKGROUND AND AIMS: Although chronic diarrhea and constipation are common, the treatment is symptomatic as their pathophysiology is poorly understood. Accumulating evidence suggests that the microbiota modulates gut function but the underlying mechanisms are unknown. We therefore investigated the pathways by which microbiota modulates gastrointestinal motility in different sections of the alimentary tract. METHODS: Gastric emptying, intestinal transit, muscle contractility, acetylcholine release, gene expression and vasoactive intestinal polypeptide (VIP) immunoreactivity were assessed in wild-type and Myd88-/- Trif-/- mice in germ-free, gnotobiotic and SPF conditions...
December 5, 2023: Cellular and Molecular Gastroenterology and Hepatology