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barcoding Diptera

Andrew Dopheide, Leah K Tooman, Stefanie Grosser, Barbara Agabiti, Birgit Rhode, Dong Xie, Mark I Stevens, Nicola Nelson, Thomas R Buckley, Alexei J Drummond, Richard D Newcomb
Invertebrates are a major component of terrestrial ecosystems, however estimating their biodiversity is challenging. We compiled an inventory of invertebrate biodiversity along an elevation gradient on the temperate forested island of Hauturu, New Zealand, by DNA barcoding of specimens obtained from leaf litter samples and pitfall traps. We compared the barcodes and biodiversity estimates from this dataset with those from a parallel DNA metabarcoding analysis of soil from the same locations, and with pre-existing sequences in reference databases, before exploring the use of combined datasets as a basis for estimating total invertebrate biodiversity...
February 27, 2019: Ecological Applications: a Publication of the Ecological Society of America
Zubaidah Ya'cob, Hiroyuki Takoka, Van Lun Low, Tiong Kai Tan, Mohd Sofian-Azirun
Simulium (Gomphostilbia) aziruni Takaoka, Hashim & Chen was described initially based only on a pupa and a mature larva collected from Peninsular Malaysia. Herein, we describe the morphological characters of the female of S. aziruni for the first time. It resembles those of the other members of the Simulium gombakense species-group by the genital fork with a distinct projection directed medioposteriorly from each arm and claw with a large basal tooth. Cytochrome c oxidase I (COI) barcoding analysis indicates that S...
February 23, 2019: Acta Tropica
John T Smit, Jeremy Miller
In this paper 4 species of bat ectoparasites are recorded from the island of Sint Eustatius, Dutch Caribbean. One species of true bug (Hemiptera: Polyctenidae) as well as 3 species of bat flies (Diptera: Hippoboscidae: Streblinae) are recorded. All species are photographed. The first DNA barcodes for 3 bat ectoparasite species ( Trichobius frequens, Trichobius intermedius, and Hesperoctenes fumarius) have been posted to the BOLD database; DNA barcode sequences for a fourth species ( Megistopoda aranea) are the first from a Caribbean island...
February 2019: Journal of Parasitology
Kantima Thongjued, Wilaiwan Chotigeat, Sara Bumrungsri, Phuvadol Thanakiatkrai, Thitika Kitpipit
Insect DNA barcoding is a species identification technique used in biodiversity assessment and ecological studies. However, DNA extraction can result in the loss of up to 70% of DNA. Recent research has reported that direct PCR can overcome this issue. However, the success rates could still be improved, and tissues used for direct PCR could not be reused for further genetic studies. Here, we developed a direct PCR workflow that incorporates a 2-min sample preparation in PBS-buffer step for fast and effective universal insect species identification...
February 13, 2019: Molecular Ecology Resources
Jade Savage, Art Borkent, Fenja Brodo, Jeffrey M Cumming, Gregory Curler, Douglas C Currie, Jeremy R deWaard, Joel F Gibson, Martin Hauser, Louis Laplante, Owen Lonsdale, Stephen A Marshall, James E O'Hara, Bradley J Sinclair, Jeffrey H Skevington
The Canadian Diptera fauna is updated. Numbers of species currently known from Canada, total Barcode Index Numbers (BINs), and estimated numbers of undescribed or unrecorded species are provided for each family. An overview of recent changes in the systematics and Canadian faunistics of major groups is provided as well as some general information on biology and life history. A total of 116 families and 9620 described species of Canadian Diptera are reported, representing more than a 36% increase in species numbers since the last comparable assessment by JF McAlpine et al...
2019: ZooKeys
David W Langor
Based on data presented in 29 papers published in the Biota of Canada Special Issue of ZooKeys and data provided herein about Zygentoma, more than 44,100 described species of terrestrial arthropods (Arachnida, Myriapoda, Insecta, Entognatha) are now known from Canada. This represents more than a 34% increase in the number of described species reported 40 years ago (Danks 1979a). The most speciose groups are Diptera (9620 spp.), Hymenoptera (8757), and Coleoptera (8302). Less than 5% of the fauna has a natural Holarctic distribution and an additional 5...
2019: ZooKeys
Hiroyuki Takaoka, Van Lun Low, Tiong Kai Tan, Zubaidah Ya'cob, Mohd Sofian-Azirun, Chee Dhang Chen, Koon Weng Lau, Xuan Da Pham
Simulium (Gomphostilbia) yvonneae sp. nov. is described based on adults, pupae, and mature larvae from Vietnam. This new species belongs to the Simulium duolongum subgroup in the S. batoense species-group of the subgenus Gomphostilbia Enderlein. It is distinguished by having a relatively larger number of male upper-eye facets in 16 vertical columns and 16 horizontal rows and a pupal gill with eight filaments arranged as 3+(1+2)+2 from dorsal to ventral, of which two filaments of the ventral pair are 1.8 times as long as the longest filament of the middle and dorsal triplets...
December 28, 2018: Journal of Medical Entomology
Chufei Tang, Ding Yang, Patrick Grootaert
In the present paper the Oriental species of the genus Lichtwardtia Enderlein, 1912 are revised based on the type material of known species and new material from Singapore and Cambodia. A re-description and illustration of the holotype female of Lichtwardtiaziczac (Wiedemann, 1824) is given but since it has been described on the basis of a female only and its provenance India Orientalis is only a vague indication of its type locality, it is considered as a nomen dubium. All the species put as junior synonyms by Becker (1922) of L...
2018: ZooKeys
Mame Thierno Bakhoum, Mamadou Sarr, Assane Gueye Fall, Karine Huber, Moussa Fall, Mbacké Sembène, Momar Talla Seck, Karien Labuschagne, Laetitia Gardès, Mamadou Ciss, Geoffrey Gimonneau, Jérémy Bouyer, Thierry Baldet, Claire Garros
BACKGROUND: Biting midge species of the genus Culicoides Latreille (Diptera: Ceratopogonidae) comprise more than 1300 species distributed worldwide. Several species of Culicoides are vectors of various viruses that can affect animals, like the African horse sickness virus (AHSV), known to be endemic in sub-Saharan Africa. The ecological and veterinary interest of Culicoides emphasizes the need for rapid and reliable identification of vector species. However, morphology-based identification has limitations and warrants integration of molecular data...
December 3, 2018: Parasites & Vectors
Hiroyuki Takaoka, VAN Lun Low, Tiong Kai Tan, Mohd Sofian-Azirun, Chee Dhang Chen, Koon Weng Lau, Xuan DA Pham
Simulium pumatense sp. nov. is described from Vietnam, and is placed in the Simulium feuerborni species-group of the subgenus Simulium (Nevermannia) Enderlein. Its morphological characteristics include the relatively smaller numbers of the following three numerical features: inner teeth of the female mandible (15-18), minute conical processes (16) on the female cibarium, and male upper-eye facets (in 15 vertical columns and 16 horizontal rows). Keys are constructed to distinguish this species from four species of the same group in Vietnam...
November 26, 2018: Acta Tropica
John Smit, Ivo Raemakers, Kevin Beentjes
Thick-headed flies of the genus Myopa Fabricius are a common sight in early spring in Europe. Several species can be found flying among Salix catkins together with their supposed Andrena Fabricius hosts (Hymenoptera: Apidae) (John Smit observations). Despite the fact that some of these Myopa species are very common, little is known about their actual host-parasitoid associations. Only a few scattered host records can be found in the literature, most of which refer to M. testacea s.l.. Whether or not these records actually concern M...
November 15, 2018: Zootaxa
Mikko Tiusanen, Tea Huotari, Paul D N Hebert, Tommi Andersson, Ashley Asmus, Joël Bêty, Emma Davis, Jennifer Gale, Bess Hardwick, David Hik, Christian Körner, Richard B Lanctot, Maarten J J E Loonen, Rauni Partanen, Karissa Reischke, Sarah T Saalfeld, Fanny Senez-Gagnon, Paul A Smith, Ján Šulavík, Ilkka Syvänperä, Christine Urbanowicz, Sian Williams, Paul Woodard, Yulia Zaika, Tomas Roslin
Pollination is an ecosystem function of global importance. Yet, who visits the flower of specific plants, how the composition of these visitors varies in space and time, and how such variation translates into pollination services is hard to establish. The use of DNA barcodes allows us to address ecological patterns involving thousands of taxa that are difficult to identify. To clarify the regional variation in the visitor community of a wide-spread flower resource, we compared the composition of the arthropod community visiting species in the genus Dryas (mountain avens, family Rosaceae), throughout Arctic and high-alpine areas...
November 12, 2018: Molecular Ecology
Gunnar Mikalsen Kvifte, Marija IvkoviĆ
Pericoma miljenkoi sp. nov. is described based on adult males from the Plitvička jezera National Park, Croatia. Pericoma trifasciata is recorded from Croatia for the first time, and COI DNA barcodes are given from specimens collected in Germany. An emended key to adult Pericoma of the trifasciata group is presented. The Croatian Psychodidae fauna now stands at 35 species.
September 26, 2018: Zootaxa
Jelena PreradoviĆ, Andrijana AndriĆ, SneŽana RadenkoviĆ, Ljiljana ŠaŠiĆ ZoriĆ, Celeste PÉrez-baÑÓn, AndrÉs Campoy, Ante VujiĆ
plants, are mostly unknown. All known immature stages of Merodon feed on underground storage organs (bulbs, rhizomes and corms) of geophytes of the families Asparagaceae, Iridaceae and Amaryllidaceae. Of 160 known Merodon species, to date, the pupal stages have been described for only four: M. equestris (Fabricius), M. bombiformis Hull, M. luteihumerus Marcos-García, Vujić Mengual, and M. geniculatus Strobl. During field investigations in Đerdap National Park, Serbia, Merodon puparia were found in the ground near the bulbs of Ornithogalum umbellatum L...
May 16, 2018: Zootaxa
Atsuya Kodama, Koichiro Kawai, Hidetoshi Saito
A new species of the genus Tanytarsus van der Wulp, 1874 was described and illustrated based on an adult male. Tanytarsus trichovalis sp. nov. belongs to the eminulus species group, and is closely related to T. tamaundecimus Sasa, 1980, T. okuboi Sasa, 1986, and T. tonebeceus Sasa Tanaka, 2000. However, the species is distinguished from these species by the morphology of the hypopygium. We also present the first record of T. ovatus Johannsen, 1932 in Japan. In addition, DNA barcoding of 5 species including T...
May 25, 2018: Zootaxa
Wojciech GiŁka, Lauri Paasivirta, Piotr Gadawski, MichaŁ Grabowski
Tanytarsus latens sp. nov. is described from Finland (Ostrobothnia borealis, Satakunta). Both morphological and             molecular analyses indicate that T. latens belongs to the mendax species group. The adult male hypopygium of the new species resembles that of Tanytarsus occultus Brundin and of T. desertor Giłka et Paasivirta, while the molecular analysis based on the mitochondrial cytochrome oxidase (COI) gene fragment evidences that T. latens is a sister species to most of European Tanytarsus of the mendax group's core, for which the COI barcodes are known...
September 6, 2018: Zootaxa
Tenielle Cooke, Kyle Sieghard Kulenkampff, Marise Heyns, Laura Jane Heathfield
Forensic entomology aids the determination of post mortem interval based on arthropods associated with a deceased body. This relies on the accurate identification of insects that visit the body, particularly first colonisers such as Calliphoridae (Diptera). Traditional species identification though morphological keys can be challenging as immature or closely related specimens can look similar. Some of these challenges have been overcome through 'DNA barcoding', which involves the sequencing of informative regions within a species' DNA and comparison to a database of reference sequences...
October 2, 2018: Genome Génome / Conseil National de Recherches Canada
Takehiro K Katoh, Guang Zhang, Masanori J Toda, Awit Suwito, Jian-Jun Gao
The subgenus Dudaica Strand of the genus Drosophila Fallén has been known to comprise only two species: Drosophila (Dudaica) senilis Duda, 1926 (recorded from Indonesia, Philippines, Vietnam, Bhutan, and India) and D.malayana (Takada, 1976) (recorded from Malaysia). In the present study, this subgenus is revised, with D.malayana redescribed and six new species discovered and described from China, Malaysia, and Indonesia: gracilipalpis Katoh & Gao, sp. n. , puberula Katoh & Gao, sp. n. , albipalpis Katoh, Toda & Gao, sp...
2018: ZooKeys
Bilgenur Baloğlu, Esther Clews, Rudolf Meier
Background: Macroinvertebrates such as non-biting midges (Chironomidae: Diptera) are important components of freshwater ecosystems. However, they are often neglected in biodiversity and conservation research because invertebrate species richness is difficult and expensive to quantify with traditional methods. We here demonstrate that Next Generation Sequencing barcodes ("NGS barcodes") can provide relief because they allow for fast and large-scale species-level sorting of large samples at low cost...
2018: Frontiers in Zoology
Ignacio Ruiz-Arrondo, Luis M Hernández-Triana, Aleksandra Ignjatović-Ćupina, Nadya Nikolova, Javier Alfonso Garza-Hernández, Mario Alberto Rodríguez-Pérez, José A Oteo, Anthony R Fooks, Javier Lucientes Curdi
BACKGROUND: Blackflies have negative impact on public and animal health due to the haematophagous habit of females. In recent times, in some regions in Spain, blackfly outbreaks are becoming more and more frequent, threatening the public health. However, there is still a paucity of data concerning the Spanish blackfly fauna. Correct identification of species is of paramount importance in order to provide correct information on species distribution, biology and behaviour, so that control measures could be implemented appropriately...
August 13, 2018: Parasites & Vectors
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