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Kunpeng Wang, Zijian Zhang, Kai Liu, Xin Yang, Heng Zou, Jiangjiao Zhou, Xiongying Miao, Wei Chen, Li Xiong, Yu Wen
BACKGROUND: Inflammatory bowel disease (IBD) is a group of inflammatory conditions characterized by chronic inflammation of the gastrointestinal (GI) tract. Recent preclinical and clinical studies have shown the therapeutic value of photodynamic therapy with low doses of photosensitizer and/or light in colitis treatment, but the detailed mechanism is unclear. METHODS: We examined 5-aminolevulinic acid (5-ALA) mediated photodynamic therapy (PDT) in dextran sulfate sodium (DSS)-induced colitis in mice...
October 29, 2018: Photodiagnosis and Photodynamic Therapy
Ferhat Demir, Alper Han Çebi, Mukaddes Kalyoncu
Juvenile idiopathic arthritis (JIA) is the most common chronic rheumatic disease of childhood, yet its etiology is unknown. It is known that microribonucleic acids (miRNAs) play a role in immunoregulation. We aimed to evaluate the plasma expression of some candidate miRNAs that are associated with the pathogenesis of autoimmunity. Thirty-one patients diagnosed with JIA and age-sex-matched 31 healthy children were enrolled for the study. The plasma levels of four candidate miRNAs (miRNA-16, miRNA-155, miRNA-204, and miRNA-451), which are known to be associated with autoimmunity, were examined in all the subjects...
December 2018: Clinical Rheumatology
Hua Li, Jibo Wang, Xiaoru Liu, Qiang Cheng
During the pathogenetic process of varied kidney diseases, renal tubules are the major sites in response to detrimental insults, including pro-inflammatory stimuli. MicroRNA-204-5p (miR-204-5p) can be detected in the renal tubular epithelial cells in the normal kidney, its expression however is downregulated in the kidney with pathological changes. This study aimed to investigate the role of miR-204-5p in interleukin 6 (IL6)-mediated inflammatory response and chemokine production in HK-2 renal tubular cells...
August 15, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Yong Qiao, Changliang Peng, Ji Li, Dongjin Wu, Xiuwen Wang
BACKGROUND: This study was to investigate the neuroprotective effect of long noncoding RNAs Metastasis associated lung adenocarcinoma transcript-1 (lncRNA MALAT-1) in Spinal Cord Ischemic/Reperfusion Injury (SCIRI). METHODS: Quantitative real-time PCR (RT-qPCR) was used to examine the expressions of MALAT1, miR-204 and Bcl-2, while western blot was performed to examine Bcl-2. Besides, apoptosis was evaluated by the percentage of cell viability and apoptotic cells...
2018: Current Neurovascular Research
Bo Zheng, Lu Chen, Chun-Chun Pan, Jian-Zhang Wang, Guang-Rong Lu, Shou-Xing Yang, Zhan-Xiong Xue, Fang-Yan Wang, Chang-Long Xu
AIM: miRNAs have been recognized for their potential in cancer therapeutics, and multiple miRNAs were suggested to affect target genes expression. To overcome limitations of free synthetic miRNAs, such as easily degraded in biofluids and limited in cellular uptake, novel miRNAs delivery systems need to be developed. MATERIALS & METHODS: Using surface-functionalizing technique, poly(D,L-lactide-co-glycolide)/poly(L-lactide)-block-poly(ethylene glycol)-folate polymer nanoparticle (PLGA/PLA-PEG-FA) loaded with miR-204-5p (FA-NPs-miR-204) was developed...
April 1, 2018: Nanomedicine
Aleksandra Butrym, Piotr Łacina, Kazimierz Kuliczkowski, Katarzyna Bogunia-Kubik, Grzegorz Mazur
BACKGROUND: MicroRNAs (miRNAs or miRs) are small molecules known to be involved in post-transcriptional gene expression. Many of them have been shown to influence risk for various diseases. Recent studies suggest that lower expression of miR-204, a gene coding for miRNA-204, is correlated with shorter survival in patients with acute myeloid leukaemia (AML). This observation prompted us to analyse the effect of two polymorphisms of the miR-204 gene, one in the upstream flanking region (rs718447 A > G) and the other inside the gene itself (rs112062096 A > G), both also in intron 3 of the TRPM3 gene...
January 30, 2018: BMC Cancer
Erik Hilborn, Olle Stål, Andrey Alexeyenko, Agneta Jansson
AIM: To investigate the influence of estrogen, androgen, microRNAs, and genes implicated in breast cancer on the expression of HSD17B1 and HSD17B2. MATERIALS: Breast cancer cell lines ZR-75-1, MCF7, T47D, SK-BR-3, and the immortalized epithelial cell line MCF10A were used. Cells were treated either with estradiol or dihydrotestosterone for 6, 24, 48 hours, or 7 days or treated with miRNAs or siRNAs predicted to influence HSD17B expression Results and discussion...
September 22, 2017: Oncotarget
Yong Qiao, Changliang Peng, Ji Li, Dongjin Wu, Xiuwen Wang
OBJECTIVE: To explore the mechanism of RAP2C participating in spinal cord ischemia-reperfusion injury (SCII). MATERIALS AND METHODS: mRNA expressions of miR-204 and RAP2C were measured by qRT-PCR. Protein level of RAP2C was detected by Western blot. Cell apoptosis in AGE1.HN and PC12 cells was detected by Annexin V/propidium iodide assay. The effect of miR-204 knockdown or overexpression on RAP2C expression was observed by luciferase assay. RESULTS: In this experimental research, RAP2C expression in SCII group was lower than control group, while miR-204 expression was higher...
October 2017: Neurological Research
Yi-Chia Lin, Ji-Fan Lin, Te-Fu Tsai, Kuang-Yu Chou, Hung-En Chen, Thomas I-Sheng Hwang
BACKGROUND: Prostate cancer (PCa) is a leading cause of cancer-related death in men, which emphasizes the need for novel therapeutic approaches. Targeting microRNA (miRNA) has been considered as a therapeutic strategy against cancers. Human miR-204-5p potentially targeting BCL2 has been reported to be downregulated in various cancers. We hypothesized that miR-204-5p overexpression induces cancer cell apoptosis by repressing BCL2 expression. METHODS: A vector harboring mature miR-204-5p was constructed and delivered into human PCa cells...
September 2017: Asian Journal of Surgery
Shuo Zhang, Lei Gao, Asmitananda Thakur, Puyu Shi, Feng Liu, Jing Feng, Ting Wang, Yiqian Liang, Johnson J Liu, Mingwei Chen, Hui Ren
MicroRNAs (miRNAs) play a critical role in cancer development and progression. Deregulated expression of miR-204 has been reported in several cancers, but the mechanism through which miR-204 modulates human non-small cell lung cancer (NSCLC) is largely unknown. In this study, we investigate the expression and functional role of miR-204 in human NSCLC tissues and cell lines. RNA isolation, qRT-PCR, MTT, colony formation assay, cell cycle assay, cell apoptosis assay, cell migration assay, and Western blot were performed...
August 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
(no author information available yet)
[This corrects the article DOI: 10.1371/journal.pgen.1005726.].
January 2016: PLoS Genetics
Yunxia Ge, Xiaodan Yan, Yiguang Jin, Xinyu Yang, Xiang Yu, Liqing Zhou, Sichong Han, Qipeng Yuan, Ming Yang
Accumulated evidence demonstrated that long non-coding RNAs (lncRNAs) play a pivotal role in tumorigenesis. However, it is still largely unknown how these lncRNAs were regulated by small ncRNAs, such as microRNAs (miRNAs), at the post-transcriptional level. We here use lncRNA HOTTIP as an example to study how miRNAs impact lncRNAs expression and its biological significance in hepatocellular carcinoma (HCC). LncRNA HOTTIP is a vital oncogene in HCC, one of the deadliest cancers worldwide. In the current study, we identified miR-192 and miR-204 as two microRNAs (miRNAs) suppressing HOTTIP expression via the Argonaute 2 (AGO2)-mediated RNA interference (RNAi) pathway in HCC...
December 2015: PLoS Genetics
Bang-fen Pan, Chen Gao, Shu-xun Ren, Yi-bin Wang, Hai-peng Sun, Mei-yi Zhou
AIM: The mitochondrial targeted 2C-type serine/threonine protein phosphatase (PP2Cm) is encoded by the gene PPM1K and is highly conserved among vertebrates. PP2Cm plays a critical role in branched-chain amino acid catabolism and regulates cell survival. Its expression is dynamically regulated by the nutrient environment and pathological stresses. However, little is known about the molecular mechanism underlying the regulation of PPM1K gene expression. In this study, we aimed to reveal how PPM1K expression is affected by miRNA-mediated post-transcriptional regulation...
December 2015: Acta Pharmacologica Sinica
Dandan Liang, Jun Li, Yahan Wu, Lixiao Zhen, Changming Li, Man Qi, Lijie Wang, Fangfei Deng, Jian Huang, Fei Lv, Yi Liu, Xiue Ma, Zuoren Yu, Yuzhen Zhang, Yi-Han Chen
OBJECTIVES: In mammals, the heart grows by hypertrophy but not proliferation of cardiomyocytes after birth. The paucity of cardiomyocyte proliferation limits cardiac regeneration in a variety of heart diseases. To explore the efficient strategies that drive cardiomyocyte proliferation, we employed in vitro and in vivo models to investigate the function of miRNA-204, which was demonstrated to regulate the proliferation and differentiation of human cardiac progenitor cells in our previous study...
December 15, 2015: International Journal of Cardiology
Tiziana Bachetti, Eleonora Di Zanni, Roberto Ravazzolo, Isabella Ceccherini
Neuroblastoma (NB) is a rare childhood cancer of the peripheral sympathetic nervous system and accounts for approximately 10% of all pediatric tumors. Heterozygous PHOX2B mutations have been found in association with NB development in familial, sporadic and syndromic cases. In addition, the PHOX2B gene is widely over-expressed both in tumor samples and NB cell lines. Post-transcriptional gene regulation is known to be involved in mRNA stability and, in NB, microRNAs (miRNAs) seem to be responsible for altered expression of genes driving differentiation, apoptosis, and migration...
August 2015: Biochimica et Biophysica Acta
Zhiqiang Xia, Fang Liu, Jian Zhang, Li Liu
Gliomas are the most common malignant primary brain tumors in adults and exhibit a spectrum of aberrantly aggressive phenotype. Although increasing evidence indicated that the deregulation of microRNAs (miRNAs) contributes to tumorigenesis and invasion, little is known about the roles of miR-204-5p in human gliomas. In the present study, the expression of miR-204-5p in clinical glioma tissues was measured by qRT-PCR. The effects of miR-204-5p on glioma cell growth and metastasis were examined by overexpressing or inhibiting miR-204-5p...
2015: PloS One
Yang Yu, Leilei Wang, Tong Liu, Hongbo Guan
Preeclampsia is a devastating pregnancy-related syndrome characterized by the onset of hypertension, proteinuria and edema. Insufficient invasion of trophoblasts is well-known to be correlated with preeclampsia development. The present study was performed to investigate the functional role microRNA (miRNA)-204 in trophoblastic invasion in vitro. We here found that the invasive capabilities of BeWo and JEG3 trophoblast-like cells were suppressed by miR-204 mimics, whereas enhanced by its inhibitor. Matrix metalloproteinase-9 (MMP9) was first confirmed to play a role in regulating trophoblast invasion through loss- or gain-of-function experiment...
July 31, 2015: Biochemical and Biophysical Research Communications
Tao Peng, Xuhong Zhou, Min Hu, Tingting Wu
OBJECTIVE: To investigate the expression of miRNA-204 in patients with NPC and to analyze the as- sociation of the expression with the cIinicopathologicaI features and prognosis. METHOD: The expression levels of miRNA-204 in 50 patients with NPC and 40 patients with nasopharyngeal chronic inflammation were detected by using stem-loop real-time reverse transcription-polymerase chain reaction RT-PCR. Analysis of the relationship between microRNA and prognosis by Kaplan-Meier method...
November 2014: Journal of Clinical Otorhinolaryngology, Head, and Neck Surgery
Kuo Li, Qiuran Xyu, Xin Liu, Qingguang Liu, Maode Wang
OBJECTIVE: To investigate the level of microRNA-204 (miR-204) in human hepatocellular carcinoma (HCC) and its effect on the potential mechanism of tumorgenesis. METHODS: Real-time quantitative PCR (qRT-PCR) was applied to detect the expression of miR-204 in HCC (n=60) and the corresponding tumor-adjacent normal tissues. The expressions of Bcl-2 and Sirt1 were measured by immunohistochemistry (IHC). The artificial miR-204 was transiently transfected into human SMMC-7721 cells in vitro...
February 2015: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Ahmet Taner Sümbül, Bülent Göğebakan, Sercan Ergün, Erhan Yengil, Celal Yücel Batmacı, Önder Tonyalı, Mehmet Yaldız
MicroRNAs (miRNAs) are important factors during tumorigenesis by affecting posttranscriptional gene expression. miRNA 204 (miR-204) is a miRNA frequently investigated in different types of cancers. According to literature, autophagy has dual roles in cancer, acting as both a tumor suppressor and cell survival agent. Also, the current data suggests that autophagy is activated in human colorectal cancer cells and enhances the aggressiveness of human colorectal cancer cells. So, our aim is to investigate potential effect of miR-204-5p on colorectal cancer by associating its expression with autophagy-related targets of miR-204-5p...
December 2014: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
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