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https://read.qxmd.com/read/30800252/development-of-one-step-tetra-primer-arms-pcr-for-simultaneous-detection-of-the-angiotensin-converting-enzyme-ace-i-d-and-rs4343-gene-polymorphisms-and-the-correlation-with-cad-patients
#1
Mohammad Mehdi Heidari, Mehdi Hadadzadeh, Hossein Fallahzadeh
Background: The Angiotensin Converting Enzyme (ACE) Insertion/Deletion and rs-4343 gene polymorphisms could be associated with pathogenesis of essential hypertension and cardiovascular disorders and Coronary Artery Disease (CAD). In the present study, a fast and novel approach of multiplex Tetra-Primer Amplification Refractory Mutation System-PCR (T-ARMS-PCR) was developed for simultaneous detection of two SNPs including ACE I/D (rs4340) and 2350A>G (rs4343) of Angiotensin Converting Enzyme ( ACE ) gene...
January 2019: Avicenna Journal of Medical Biotechnology
https://read.qxmd.com/read/30702112/novel-adamantane-based-hole-transport-materials-for-perovskite-solar-cells-a-computational-approach
#2
Maebienne Anjelica B Gapol, Dong Hee Kim
Adamantane derivatives have been subjected to quantum mechanical calculations to determine their capabilities as potential hole transport materials (HTMs) in perovskite solar cells (PSCs). Adamantane has been modified in two ways: multiple arm substitution and outermost substituent variation. It has been shown that tetra-substitution of adamantane gave the best characteristics as a HTM. Further modification showed tetra-ethyl substituted adamantane (ad-EtTPA) has the lowest HOMO, a small hole reorganization energy (λh), absorption in the UV region, and good stability...
January 31, 2019: Physical Chemistry Chemical Physics: PCCP
https://read.qxmd.com/read/30674826/evaluation-of-mesh-size-in-model-polymer-networks-consisting-of-tetra-arm-and-linear-poly-ethylene-glycol-s
#3
Yui Tsuji, Xiang Li, Mitsuhiro Shibayama
The structure and mechanical properties of model polymer networks consisting of alternating tetra-functional poly(ethylene glycol)s (PEGs) and bis-functional linear PEGs were investigated by dynamic light scattering and rheological measurements. The sizes of the correlation blob ( ξ c ) and the elastic blob ( ξ e l ) were obtained from these measurements and compared to the theoretical mesh size, the geometric blob ( ξ g ), calculated by using the tree-like approximation. By fixing the concentration of tetra-PEGs and tuning the molecular weight of linear-PEGs, we systematically compared these blob sizes in two cases: complete network (Case A) and incomplete network (Case B)...
May 25, 2018: Gels
https://read.qxmd.com/read/30671185/evaluation-of-fto-rs9939609-and-mc4r-rs17782313-polymorphisms-as-prognostic-biomarkers-of-obesity-a-population-based-cross-sectional-study
#4
Mina Mozafarizadeh, Mohsen Mohammadi, Soha Sadeghi, Morteza Hadizadeh, Tayebe Talebzade, Massoud Houshmand
Objectives: Obesity is a significant risk factor for a number of chronic diseases, including diabetes, cardiovascular diseases, and cancer. Obesity usually results from a combination of causes and contributing factors, including genetics and lifestyle choices. Many studies have shown an association of single nucleotide polymorphisms (SNPs) in the fat mass and obesity-associated ( FTO ) and the melanocortin-4 receptor ( MC4R ) genes with body mass index (BMI). Therefore, recognizing the main genes and their relevant genetic variants will aid prediction of obesity risk...
January 2019: Oman Medical Journal
https://read.qxmd.com/read/30656224/rapid-and-reliable-identification-of-insulin-2-gene-mutation-in-akita-diabetic-mice-by-a-tetra-primer-arms-pcr-method
#5
Rambabu Undi, Hui-Ying Lim, Weidong Wang
The Akita mouse, one of the most frequently used animal models for the study of diabetes mellitus and its complications, carries a heterozygous missense mutation (C96Y) in the insulin 2 (Ins2) gene that results in proinsulin misfolding in the endoplasmic reticulum (ER), ER stress, pancreatic beta cell death and ultimately diabetes. Maintenance of Akita mice entails genotyping for the identification of the heterozygous Akita mutation. Current genotyping methods for the Akita mouse strain are time consuming, expensive, or needing special device...
January 2019: Heliyon
https://read.qxmd.com/read/30637283/alternating-thalamic-deep-brain-stimulation-for-essential-tremor-a-trial-to-reduce-habituation
#6
Mara Seier, Amie Hiller, Joseph Quinn, Charles Murchison, Matthew Brodsky, Shannon Anderson
Background: DBS in the ventral intermediate nucleus (VIM) of the thalamus has been a revolutionary treatment for patients with essential tremor (ET) by reducing tremor. Unfortunately, some patients develop habituation to DBS and thus experience reduced efficacy and loss of tremor control. There are no standardized methods of addressing habituation to DBS. We propose alternating stimulation patterns as a way to reduce habituation. Methods: This was a randomized, placebo-controlled trial for patients with VIM DBS for ET...
November 2018: Movement Disorders Clinical Practice
https://read.qxmd.com/read/30626189/microfluidic-formation-of-hydrogel-microcapsules-with-a-single-aqueous-core-by-spontaneous-cross-linking-in-aqueous-two-phase-system-atps-droplets
#7
Takaichi Watanabe, Ibuki Motohiro, Tsutomu Ono
We report a simple process to fabricate monodisperse tetra-arm poly(ethylene glycol) (tetra-PEG) hydrogel microcapsules with an aqueous core and a semi-permeable hydrogel shell through the formation of aqueous two-phase system (ATPS) droplets consisting of dextran (DEX)-rich core and tetra-PEG macromonomer-rich shell, followed by spontaneous cross-end coupling reaction of tetra-PEG macromonomers in the shell. Different from conventional techniques, this process enables for the continuous production of hydrogel microcapsules from water-in-oil emulsion droplets under mild conditions in the absence of radical initiators and external stimuli such as heating and ultraviolet (UV) light irradiation...
January 10, 2019: Langmuir: the ACS Journal of Surfaces and Colloids
https://read.qxmd.com/read/30597236/establishment-and-validation-of-arms-amplification-refractory-mutation-system-for-identification-of-anisakis-species-collected-from-korean-waters
#8
Hyunsu Kim, Kyung-Wan Baek, Mi-Kyung Park, Kyung-Yoon Jeon, Eun-Ji Ko, Hee-Jae Cha, Mee Sun Ock
The identification of Anisakis species in Korean waters was performed using an amplification-refractory mutation system (ARMS). ARMS is typically used to detect single nucleotide polymorphisms or allele types in the same species. However, the application of ARMS for species identification has not been reported. We designed a tetra-primer binding the internal transcribed spacer (ITS) region of 76 samples of Anisakis species and obtained reproducible results. ARMS revealed 380 bp and 130 bp ITS fragments in A...
December 28, 2018: Gene
https://read.qxmd.com/read/30583668/analysis-of-polymorphism-and-expression-profile-of-asic1-and-il-6-genes-in-patients-with-gastric-cancer
#9
Abdulkuddous Heydari-Mehrabadi, Dor Mohammad Kordi Tamandani, Taybeh Baranzehi, Simin Hemati
Gastric cancer is one of the most common upper gastrointestinal malignancies. Some Iranian provinces, such as in the northern and northwestern areas, are at a high risk, whereas the central and western provinces are at a medium and the southern regions at low risk. This study was carried out to estimate the impact of the expression patterns of ASIC1 and IL-6 genes and the IL-6rs-174 and ASIC1rs 75624685 polymorphisms in the pathogenesis of gastric cancer. Materials and methods: Tetra-ARMS PCR was employed to analyze the polymorphism status of the ASIC1 and IL-6 genes with 85 paraffin-embedded tissue blocks from cases and 117 normal blood samples as controls...
December 25, 2018: Asian Pacific Journal of Cancer Prevention: APJCP
https://read.qxmd.com/read/30581791/nitric-oxide-synthase-2-polymorphisms-rs2779248t-c-and-rs1137933c-t-and-the-risk-of-type-2-diabetes-in-zahedan-southeastern-iran
#10
Yasaman Garme, Mahdiyeh Moudi, Ramin Saravani, Hamidreza Galavi
Background: Nitric oxide (NO) has been associated with insulin resistance and type 2 diabetes (T2D). NO is synthesized enzymatically from l-arginine (l-Arg) by three NO synthase (NOS) isoforms, Neuronal NOS (nNOS or NOS1), Inducible NOS (iNOS or NOS2), and Endothelial NOS (eNOS or NOS3). The impact of NOS2 gene polymorphism was investigated on the susceptibility of T2D in a sample of Iranian population (Southeastern of Iran). Methods: In 2015, the present case-control study was conducted on 152 T2D patients and 157 healthy control subjects (HCs) referring to Bu-ali Hospital of Zahedan, eastern Iran...
November 2018: Iranian Journal of Public Health
https://read.qxmd.com/read/30550621/a-facile-on-bead-method-for-fully-symmetric-tetra-substituted-dota-derivatizations-using-peptoid-moieties
#11
Vineeta Rustagi, D Gomika Udugamasooriya
The metal-chelated 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) has made a significant impact on the field of diagnostic imaging. This imaging mechanism is largely dependent on the four side arm functionalities around the DOTA scaffold. We previously demonstrated the effect of peptoid residue modification on these DOTA side arms, thereby conferring diverse physiochemical properties to the imaging mechanism. We generated two on-bead Eu(III)-DOTA libraries with three side arm modifications, where the remaining arm was used to attach DOTA onto the resin...
December 14, 2018: Biopolymers
https://read.qxmd.com/read/30515698/significant-association-of-tox3-loc643714-locus-rs3803662-and-breast-cancer-risk-in-a-cohort-of-iranian-population
#12
Amir Tajbakhsh, Fahimeh Afzal Javan, Mahdi Rivandi, Atefeh Moezzi, Soheila Abedini, Mahla Asghari, Zahra Farjami, Hosein Soltanian, Fatemeh Homaei Shandiz, Mohammad Mahdi Kooshyar, Alireza Pasdar
Genome-wide association studies normally focus on low penetrance and moderate to high-frequency single nucleotide polymorphisms (SNPs), which lead to genetic susceptibility to breast cancer. In this regard, the T allele of rs3803662 has been associated with breast cancer risk and with lower expression level of TOX3. We aimed to assess the risk of breast cancer associated with this polymorphism in an Iranian population. Using Tetra Primer ARMS PCR, rs3803662 was analyzed in a total of 943 individuals (430 cases and 513 healthy controls form North East of Iran)...
December 4, 2018: Molecular Biology Reports
https://read.qxmd.com/read/30513588/the-er-stress-inducer-l-azetidine-2-carboxylic-acid-elevates-the-levels-of-phospho-eif2%C3%AE-and-of-lc3-ii-in-a-ca-2-dependent-manner
#13
Gemma Roest, Evelien Hesemans, Kirsten Welkenhuyzen, Tomas Luyten, Nikolai Engedal, Geert Bultynck, Jan B Parys
Accumulation of misfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) to reduce protein load and restore homeostasis, including via induction of autophagy. We used the proline analogue l-azetidine-2-carboxylic acid (AZC) to induce ER stress, and assessed its effect on autophagy and Ca2+ homeostasis. Treatment with 5 mM AZC did not induce poly adenosine diphosphate ribose polymerase (PARP) cleavage while levels of binding immunoglobulin protein (BiP) and phosphorylated eukaryotic translation initiation factor 2α (eIF2α) increased and those of activating transcription factor 6 (ATF6) decreased, indicating activation of the protein kinase RNA-like ER kinase (PERK) and the ATF6 arms of the UPR but not of apoptosis...
November 30, 2018: Cells
https://read.qxmd.com/read/30478747/development-and-application-of-low-cost-t-arms-pcr-assay-for-agt-and-cyp11b1-gene-polymorphisms
#14
Misbah Hussain, Haq Nawaz Khan, Fazli Rabbi Awan
Angiotensin II (Ang II: a truncated octapeptide of angiotensinogen, AGT) and 11-β-hydroxylase influence regulation of blood pressure. Dysregulation of Ang II and 11-β-hydroxylase can lead to hypertension and elevate aldosterone levels. Polymorphisms in AGT (encodes AGT) and CYP11B1 (encodes 11-β-hydroxylase) shift the paradigm from physiological to pathological. Currently, various high-throughput techniques are used to genotype these polymorphisms. These techniques require expensive infrastructure and reagents...
November 26, 2018: Molecular Biology Reports
https://read.qxmd.com/read/30474684/strategic-synthesis-of-cu-2-cu-4-and-cu-5-complexes-inhibition-and-triggering-of-ligand-arm-hydrolysis-and-self-aggregation-by-chosen-ancillary-bridges
#15
Manisha Das, Angelos B Canaj, Valerio Bertolasi, Mark Murrie, Debashis Ray
The Schiff base ligand HL1 ({2,6-bis(allylimino)methyl}-4-methylphenol) having no coordinating donor arm has been examined for its reaction medium and ancillary bridge dependent reactivity for a hierarchical family of CuII complexes. The ligand showed a unique reactivity pattern toward CuII in solution. The bridging nature of in situ generated HO- ions in the absence and presence of externally added carboxylates (RCOO-; R = CF3, C6H5 and CH3) has been utilized to produce complexes {[Cu2(μ-L2)2(H2O)]2[Cu2(μ-L2)2(H2O)2](ClO4)6} (1) (HL2 = 3-{(allylimino)methyl}-2-hydroxy-5-methylbenzaldehyde), [Cu4(μ4-O)(μ-L1)2(μ1,3-O2CCF3)4] (2), [Cu4(μ4-O)(μ-L1)2(μ1,3-O2CC6H5)4]·H2O (3), and [Cu5(μ3-OH)2(μ-L1)2(μ1,3-OAc)2(OAc)2(H2O)4][Cu5(μ3-OH)2(μ-L1)2(μ1,3-OAc)2(OAc)3(H2O)](ClO4)3·2C2H5OH (4)...
November 26, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
https://read.qxmd.com/read/30456721/association-of-shmt1-maz-erg-and-l3mbtl3-gene-polymorphisms-with-susceptibility-to-multiple-sclerosis
#16
Seyede Zahra Nazari Mehrabani, Mohammad Hossein Shushizadeh, Mohammad Foad Abazari, Maryam Nouri Aleagha, Abbas Ardalan, Rasoul Abdollahzadeh, Asaad Azarnezhad
Multiple sclerosis (MS) is the most common inflammatory and chronic disease of the central nervous system (CNS). A complex interaction between genetic, environmental, and epigenetic factors is involved in the pathogenesis of MS. With the advancement of GWAS, various variants associated with MS have been identified. This study aimed to evaluate the association of single-nucleotide polymorphisms (SNPs) rs4925166 and rs1979277 in the SHMT1, MAZ rs34286592, ERG rs2836425, and L3MBTL3 rs4364506 with MS. In this case-control study, the association of five SNPs in SHMT1, MAZ, ERG, and L3MBTL3 genes with relapsing-remitting MS (RR-MS) was investigated in 190 patients and 200 healthy individuals...
November 19, 2018: Biochemical Genetics
https://read.qxmd.com/read/30447310/interleukin-33-gene-expression-and-rs1342326-polymorphism-in-beh%C3%A3-et-s-disease
#17
Mahsa Talei, Ali Abdi, Dariush Shanebandi, Farhad Jadidi-Niaragh, Alireza Khabazi, Farhad Babaie, Shahriar Alipour, Babak Afkari, Ebrahim Sakhinia, Zohreh Babaloo
OBJECTIVE: Behçet's disease (BD) is a chronic multi-factorial inflammatory disease with the important role of genetic in activation of its inflammatory response. Interleukin (IL)-33 is a member of the IL-1 family of cytokines that affects innate and adaptive immune systems to promote inflammatory responses. In the current study, we investigated the association of IL-33 gene rs1342326 polymorphism and expression levels of this gene in peripheral blood mononuclear cells (PBMCs) with the susceptibility to BD in Azari population of Iran...
November 14, 2018: Immunology Letters
https://read.qxmd.com/read/30430339/breast-cancer-risk-associated-with-brca1-2-variants-in-the-pakistani-population
#18
Saba Abbas, Ayesha Siddique, Naeem Shahid, Rabbia Tariq Khan, Warda Fatima
BACKGROUND: Majority of the BRCA1 and BRCA2 mutations are associated with the risk of sporadic and familial breast cancer. Since these genes are significant in DNA repair mechanisms, we focused homology-directed DNA repair (HDDR) and BRCA complex. METHODS: We selected BRCA1 variant (rs80356932, 4491C/T) and BRCA2 variant (rs80359182, 319T/C) from the interaction region of BRCA complex and studied in 100 breast cancer patients and 100 controls using tetra-ARMS-PCR...
November 14, 2018: Breast Cancer: the Journal of the Japanese Breast Cancer Society
https://read.qxmd.com/read/30410168/association-of-vaspin-levels-and-its-snp-rs2236242-with-gestational-diabetes-at-a-tertiary-care-setting-research-article
#19
Karishma Kanhya Lal, Rabail Jarwar, Sabah Farhat, Seyda Sadia Fatima
OBJECTIVE: To evaluate and correlate vaspin levels and genotype frequency in gestational diabetes mellitus. METHODS: The case-control study was conducted at Aga Khan University, Karachi, from November 2015 to December 2016, and comprised pregnant women in their second trimester with gestational diabetes mellitus. Healthy pregnant women with similar characteristics were enrolled as the control group. Tetra arms amplification system for vaspin gene was performed. SPSS 21 was used for data analysis...
November 2018: JPMA. the Journal of the Pakistan Medical Association
https://read.qxmd.com/read/30390221/efficacy-and-safety-of-cannabidiol-in-epilepsy-a-systematic-review-and-meta-analysis
#20
REVIEW
Simona Lattanzi, Francesco Brigo, Eugen Trinka, Gaetano Zaccara, Claudia Cagnetti, Cinzia Del Giovane, Mauro Silvestrini
BACKGROUND: Approximately one-third of patients with epilepsy presents seizures despite adequate treatment. Hence, there is the need to search for new therapeutic options. Cannabidiol (CBD) is a major chemical component of the resin of Cannabis sativa plant, most commonly known as marijuana. The anti-seizure properties of CBD do not relate to the direct action on cannabinoid receptors, but are mediated by a multitude of mechanisms that include the agonist and antagonist effects on ionic channels, neurotransmitter transporters, and multiple 7-transmembrane receptors...
November 2018: Drugs
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