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Lactobacillus Genome

Jeong Eun Kwon, Jaewon Lim, Ina Bang, Inhye Kim, Donghyuk Kim, Se Chan Kang
BACKGROUND: Equol, major isoflavone metabolite, -producing bacteria have been isolated and characterized, however fermentation has been performed with soybean-based products as substrates. Pueraria lobata has been reported as a plant with higher content of isoflavones. RESULTS: The genome of new equol-producing bacteria, Lactobacillus paracasei JS1, was analyzed. Also, the effect of P. lobata extract fermented with L. paracasei JS1 (FPE) on the skin and intestinal immune response was examined...
February 14, 2019: Journal of the Science of Food and Agriculture
E Scaltriti, D Carminati, C Cortimiglia, R Ramoni, K I Sørensen, G Giraffa, M Zago
Lactobacillus helveticus is a homofermentative thermophilic LAB that is mainly used in the manufacture of Swiss type and long-ripened Italian hard cheeses. In this study, the presence of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) were analysed in 25 L. helveticus genomes and identified in 23 of these genomes. A total of 40 CRISPR loci were identified and classified into five main families based on CRISPR repeats: Ldbu1, Lsal1, Lhel1, Lhel2 and a new repeat family named Lhel3. Spacers had a size between 30 and 40 bp whereas repeats have an average size of 30 bp, with three longer repeats...
February 14, 2019: Letters in Applied Microbiology
Soumi De Montijo-Prieto, David J Castro, Jose C Reina, Maria Jimenez-Valera, Alfonso Ruiz-Bravo
Lactobacillus plantarum C4 (CECT 9567) was isolated from kefir and has been extensively studied because of its probiotic properties. Here we report the genome sequence of this strain. The genome consists of 3,221,350 bp, and contains 3058 CDSs with an average G + C content of 44.5%. The genome harbors genes encoding the AraC-family transcription regulator, the penicillin-binding protein Pbp2A, and the Na+/H+ antiporter NapA3, which have important roles in the survival of lactobacilli in the gastrointestinal tract...
February 13, 2019: Archives of Microbiology
Wilson José Fernandes Lemos Junior, André Fioravante Guerra, Vinícius da Silva Duarte, Laura Treu, Armin Tarrah, Stefano Campanaro, Rosa Helena Luchese, Alessio Giacomini, Viviana Corich
Here the draft genome sequence of Lactobacillus paracasei strain DTA83, isolated from stools of healthy infants in Rio de Janeiro (Brazil), is reported. The 2.8-Mb genome possesses 2825 protein-coding sequences distributed on 330 SEED subsystems. This strain belongs to a set of potentially probiotic Lactobacillus spp. strains used to study genetic factors related to antibiotic resistance after stress conditions, such as simulated gastrointestinal conditions. The complete genome data have been deposited in GenBank under the accession number QRBH00000000, https://www...
February 2019: Data in Brief
Yifei Zhang, Qian Zhang
Background: Antibiotic resistance, is often conferred by the presence of antibiotic resistance genes. This study aimed to investigate the relationship between tetracycline resistance (Tet-R) and genotype in 31 Lactobacillus isolates from caries-active patients. Methods: The tetracycline susceptibility of Lactobacillus isolates was determined using the agar spot test and the genetic characteristics associated with tetracycline resistance using whole-genome sequencing (WGS)...
2019: Antimicrobial Resistance and Infection Control
Surya Chandra Rao Thumu, Prakash M Halami
Three strains of Lactobacillus comprising Lactobacillus salivarius (CHS-1E and CH7-1E) and Lactobacillus reuteri (CH2-2) previously isolated from chicken meat were analyzed for their transferability of antibiotic resistance (AR) genes to pathogenic strains under in vivo, in vitro, and during food fermentation. For in vivo model, Albino Wistar rats were inoculated with 1010  CFU/g/ml of Enterococcus faecalis JH2-2 (recipient). After 7 days, either of two donors L. salivarius CH7-1E or L. reuteri [harbouring erythromycin and tetracycline resistance genes] were introduced at a concentration of 109  CFU/ml daily for 1 week...
February 2019: Food Research International
Dayou Shi, Lin Bai, Qian Qu, Shanshan Zhou, Meimei Yang, Shining Guo, Qiuhong Li, Cui Liu
Gut microbiota** play important roles in the health and disease status of both humans and animals. Little is known about whether heat stress changes the composition of the gut microbiota in chicken. The aim of this study was to investigate the effects of heat stress on changes in caecal microbiota, including changes in growth performance as well as HSP70 and cortisol levels. Sixty 14-day-old female broilers were equally divided into 2 treatment groups with different housing temperatures for 28 D: a control group (C) at 24 to 26°C and a heat stress (HS) group at 34 to 38°C...
February 1, 2019: Poultry Science
Elias McComb, Johanna Holm, Bing Ma, Jacques Ravel
Lactobacillus crispatus is a commonly found bacterium in vertebrate microbiota, particularly the human vagina. We report the first complete genome of a strain isolated from a human vagina, L. crispatus CO3MRSI1.
January 2019: Microbiology resource announcements
Ting Yu, Xi Yang, Zhilin Wang, Cui Zhu, Jinlong Bei, Li Wang, Wenhua Liu, Qingfeng Zhu, Zongyong Jiang, Zhuang Chen
Lactobacillus reuteri strain LR CGMCC 11154, which was isolated from the feces of healthy weaned piglets, was experimentally proven to be a probiotic bacterium. The whole genome was sequenced on the Illumina Miseq platform to obtain the draft genome, which consists of 120 contigs totaling 1.9 Mbp encoding 1,854 genes.
January 2019: Microbiology resource announcements
Laura M Alexander, Jee-Hwan Oh, Donald S Stapleton, Kathryn L Schueler, Mark P Keller, Alan D Attie, Jan-Peter van Pijkeren
Lactobacillus reuteri has the potential to be developed as a microbial therapeutic delivery platform because of an established safety profile, health-promoting properties, and available genome editing tools. Here, we showed that L. reuteri VPL1014 exhibits a low mutation rate compared to other gram-positive bacteria, which we expect will contribute to the stability of genetically modified strains. VPL1014 encodes two biologically active prophages, which are induced during GI transit. We hypothesized that intracellular accumulated recombinant protein can be delivered in situ following bacteriophage-mediated lysis...
January 25, 2019: Applied and Environmental Microbiology
Jia Sin Ong, Todd D Taylor, Cheng Chung Yong, Boon Yin Khoo, Sreenivasan Sasidharan, Sy Bing Choi, Hiroshi Ohno, Min Tze Liong
This study aimed to elucidate the targets and mechanisms of anti-staphylococcal effects from bioactive metabolites produced by lactic acid bacteria. We aimed to better understand the safety and efficacy of these bioactive metabolites in in vivo systems, typically at topical sites. The cell-free supernatant and protein-rich fraction from Lactobacillus plantarum USM8613 inhibited staphyloxanthin biosynthesis, reduced (p < 0.05) the cell number of Staphylococcus aureus by 106  CFU/mL and reduced biofilm thickness by 55% in S...
January 18, 2019: Probiotics and Antimicrobial Proteins
Nilusha Malmuthuge, Guanxiang Liang, Philip J Griebel, Le Luo Guan
A lack of information on the intestinal microbiome of neonatal calves prevents the use of microbial intervention strategies to improve calf gut health. This study profiled the taxonomic and functional composition of the small intestinal luminal microbiome of neonatal calves using whole genome sequencing of metagenome, aiming to understand the dynamics of microbial establishment during the early life. Despite highly individualized microbial communities, we identified two distinct taxonomic-based clusters from the collective luminal microbiomes comprising either a high level of Lactobacillus or Bacteroides Among the clustered microbiomes, Lactobacillus -dominant ileal microbiomes had significantly lower abundances of Bacteroides , Prevotella , Roseburia , Ruminococcus , and Veillonella compared to the Bacteroides -dominated ileal microbiomes...
January 18, 2019: Applied and Environmental Microbiology
Jee-Hwan Oh, Laura M Alexander, Meichen Pan, Kathryn L Schueler, Mark P Keller, Alan D Attie, Jens Walter, Jan-Peter van Pijkeren
The mammalian intestinal tract contains a complex microbial ecosystem with many lysogens, which are bacteria containing dormant phages (prophages) inserted within their genomes. Approximately half of intestinal viruses are derived from lysogens, suggesting that these bacteria encounter triggers that promote phage production. We show that prophages of the gut symbiont Lactobacillus reuteri are activated during gastrointestinal transit and that phage production is further increased in response to a fructose-enriched diet...
December 24, 2018: Cell Host & Microbe
Xiao-Pei Chao, Ting-Ting Sun, Shu Wang, Qing-Bo Fan, Hong-Hui Shi, Lan Zhu, Jing-He Lang
OBJECTIVES: Since other genital infections enhance HIV susceptibility by inducing inflammation and evidence suggests that the vaginal microbiome plays a functional role in the persistence or regression of high-risk human papillomavirus (HPV) infections, we investigated the relationship between the composition of the vaginal microbiota and the risk of high-risk HPV infection. METHODS: The study included 151 healthy women (65 HPV-positive and 86 HPV-negative) aged 20-65 at enrollment...
January 2019: International Journal of Gynecological Cancer
Julian A Schuster, Rudi F Vogel, Matthias A Ehrmann
Clustered regularly interspaced palindromic repeats (CRISPR)-Cas (CRISPR-associated) structures, known as prokaryotes 'immune system', have been successfully applied for genetic engineering and genotyping purposes for a variety of microorganisms. Here we investigated 50 Lactobacillus (L.) sakei genomes and found 13 of them as CRISPR-Cas positive. The majority of positive genomes contain type II-A system, which appears to be widespread across food born lactic acid bacteria. However, a type II-C system with low similarity in Cas protein sequence to related II-C structures is rarely present in the genomes...
January 11, 2019: Archives of Microbiology
Masanori Tohno, Yasuhiro Tanizawa, Yoichiro Kojima, Mitsuo Sakamoto, Yasukazu Nakamura, Moriya Ohkuma, Hisami Kobayashi
A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, strain YK43T , isolated from spent mushroom substrates stored in Nagano, Japan was performed. Growth was detected at 15-45 °C, pH 5.0-8.5, and 0-10 % (w/v) NaCl. The genomic DNA G+C content of strain YK43T was 43.6 mol%. The predominant fatty acids were C16 : 0, C18 : 1 ω9c and summed feature 8. Based on 16S rRNA gene sequence analysis, the type strains of Lactobacillus acidipiscis (sequence similarity, 97...
January 10, 2019: International Journal of Systematic and Evolutionary Microbiology
Iulian A Grosu, Gina C Pistol, Ionelia Taranu, Daniela E Marin
The study investigated the effect of grape seed (GS) meal, aflatoxin (AFB1), or their combination on the large intestine microbiota of weanling piglets. Twenty-four piglets were allocated into four groups based on diet composition: (1) Control group; (2) AFB1 (320 g/kg feed) group; (3) GS group (8% inclusion in the diet); (4) AFB1 + GS group. After 30 days of experiment, the colon content was used for microbiota analyses; after isolation of total bacterial genomic DNA, V3/V4 regions of the 16S rRNA amplicons were sequenced using the Illumina MiSeq platform...
January 8, 2019: Toxins
Ilaria Carafa, Giorgia Stocco, Piero Franceschi, Andrea Summer, Kieran Michael Tuohy, Giovanni Bittante, Elena Franciosi
Lactococcus lactis subsp. lactis 68, Streptococcus thermophilus 93 and Lactobacillus rhamnosus BT68, previously isolated from Traditional Mountain (TM) cheese, were tested for the production of four experimental mountain cheeses, with the aim to assess their effectiveness in leading the TM-cheese-making process. Lactococcus lactis subsp. lactis 68 and Streptococcus thermophilus 93 were used as starter cultures, whereas Lactobacillus rhamnosus BT68 was used as non-starter culture. Three control (CTRL) cheeses were manufactured without adding any starter, according to the traditional cheese-making process; nine, cheeses were produced inoculating the vat milk with the starters (ST), starter and low concentration of non-starter culture (STLC), starter and high concentration of non-starter culture (STHC)...
January 2019: Food Research International
Xiaoqun Zeng, Qing Pan, Yuxing Guo, Zhen Wu, Yangying Sun, Yali Dang, Jinxuan Cao, Jun He, Daodong Pan
Nitrite helps meat products retain colour and prevents decay, but excessive intake can be carcinogenic. To investigate the mechanism of nitrite degradation by lactic acid bacteria, Lactobacillus fermentum RC4 were grown in broth containing NaNO2 at 0, 100, 300, or 500 mg/L, and 8-plex iTRAQ proteomics and bioinformatics analysis were employed to explore protein expression patterns during nitrite degradation. Real-time PCR was used to confirm mRNA expression levels of selected genes. In the 100, 300, and 500 mg/L sodium nitrite groups, 31, 87, and 190 differentially expressed proteins (DEPs) were identified, of which 24, 57, and 109 were up-regulated and 7, 30, and 81 were down-regulated...
December 21, 2018: Journal of Proteomics
Michael J Davoren, Jared Liu, Jocelyn Castellanos, Norma I Rodríguez-Malavé, Robert H Schiestl
Probiotics are considered to have multiple beneficial effects on the human gastrointestinal tract, including immunomodulation, pathogen inhibition, and improved host nutrient metabolism. However, extensive characterization of these properties is needed to define suitable clinical applications for probiotic candidates. Lactobacillus johnsonii 456 (LBJ 456) was previously demonstrated to have anti-inflammatory and anti-genotoxic effects in a mouse model. Here, we characterize its resistance to gastric and bile acids as well as its ability to inhibit gut pathogens and adhere to host mucosa...
December 22, 2018: Gut Microbes
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