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human adenovirus infection

Fengling Feng, Jin Zhao, Pingchao Li, Ruiting Li, Ling Chen, Caijun Sun
Antigen-specific T lymphocytes play a critical role in controlling viral infections. However, we report here that preexisting virus-specific T cell responses also contribute to promoting adenovirus (Ad) infection. Previously, we found that CD14+ monocytes from Ad-seropositive individuals exhibited an increased susceptibility to Ad infection, when compared with that of Ad-seronegative individuals. But the underlying mechanisms for this enhancement of viral infection are not completely clarified. In this study, we found that the efficacy of Ad infection into CD14+ monocytes was significantly decreased after CD3+ T lymphocytes depletion from PBMC samples of Ad-seropositive individuals...
February 13, 2019: Viruses
Musa Hindiyeh, Orna Mor, Rakefet Pando, Batya Mannasse, Areej Kabat, Hadar Assraf-Zarfati, Ella Mendelson, Danit Sofer, Michal Mandelboim
Respiratory viral infections constitute the majority of samples tested in the clinical virology laboratory during the winter season, and are mainly diagnosed using molecular assays, namely real-time PCR (qPCR). Therefore, a high-quality extraction process is critical for successful, reliable and sensitive qPCR results. Here we aimed to evaluate the performance of the newly launched eMAG compared to the fully automated MagNA PURE 96 (Roche, Germany) and to the semi-automated easyMAG (bioMerieux, France) extraction platforms...
2019: PloS One
Sunčanica Ljubin-Sternak, Tomislav Meštrović, Irena Ivković-Jureković, Goran Tešović, Gordana Mlinarić-Galinović, Ivana Lukšić, Irena Tabain, Tatjana Tot, Maja Mijač, Jasmina Vraneš
BACKGROUND: Human bocavirus (HBoV) is known to cause lower respiratory tract infections (LRTI) in children and may result in substantial morbidity and mortality. The aim of this study was to determine HBoV prevalence among hospitalized infants and small children with acute LRTI in Zagreb, Croatia, as well as to evaluate HBoV DNA quantity in samples in relation to the patients' age and co-infection with other respiratory viruses. METHODS: During winter season 2016/2017, a total of 295 children younger than three years of age who were admitted to hospitals with LRTI were tested for the presence of HBoV, respiratory syncytial virus (RSV), adenovirus (ADV), parainfluenza virus (PIV) types 1 to 3, and human metapneumovirus (HMPV)...
January 1, 2019: Clinical Laboratory
Weiwei Wang, Yanni Yu, Yi Jiang, Jing Qu, Longxing Niu, Jicheng Yang, Mingzhong Li
Vascularization remains a critical challenge in dermal tissue regeneration. In this study, a vascular endothelial growth factor (VEGF165) and angiopoietin-1 (Ang-1) dual gene co-expression vector that encoded green fluorescent protein (GFP) was constructed from an Arg-Gly-Asp-modified adenovirus. Silk fibroin (SF) scaffolds loaded with adenovirus vectors were fabricated by freeze-drying method. In vitro, the human endothelial-derived cell line EA.hy926 was infected with adenovirus vectors and then expressed GFP, secreted VEGF165 and Ang-1, and promoted cell proliferation effectively...
February 15, 2019: Journal of Tissue Engineering and Regenerative Medicine
Ge Li, Sandra Bos, Konstantin A Tsetsarkin, Alexander G Pletnev, Philippe Desprès, Gilles Gadea, Richard Y Zhao
The Zika virus (ZIKV) was first isolated in Africa in 1947. It was shown to be a mild virus that had limited threat to humans. However, the resurgence of the ZIKV in the most recent Brazil outbreak surprised us because it causes severe human congenital and neurologic disorders including microcephaly in newborns and Guillain-Barré syndrome in adults. Studies showed that the epidemic ZIKV strains are phenotypically different from the historic strains, suggesting that the epidemic ZIKV has acquired mutations associated with the altered viral pathogenicity...
February 14, 2019: Viruses
Alexander T Baker, Alexander Greenshields-Watson, Lynda Coughlan, James A Davies, Hanni Uusi-Kerttula, David K Cole, Pierre J Rizkallah, Alan L Parker
Adenovirus based vectors are of increasing importance for wide ranging therapeutic applications. As vaccines, vectors derived from human adenovirus species D serotypes 26 and 48 (HAdV-D26/48) are demonstrating promising efficacy as protective platforms against infectious diseases. Significant clinical progress has been made, yet definitive studies underpinning mechanisms of entry, infection, and receptor usage are currently lacking. Here, we perform structural and biological analysis of the receptor binding fiber-knob protein of HAdV-D26/48, reporting crystal structures, and modelling putative interactions with two previously suggested attachment receptors, CD46 and Coxsackie and Adenovirus Receptor (CAR)...
February 14, 2019: Nature Communications
Christian R Kahrs, Katerina Chuda, German Tapia, Lars C Stene, Karl Mårild, Trond Rasmussen, Kjersti S Rønningen, Knut E A Lundin, Lenka Kramna, Ondrej Cinek, Ketil Størdal
OBJECTIVE: To determine whether infection with human enterovirus or adenovirus, both common intestinal viruses, predicts development of coeliac disease. DESIGN: Case-control study nested within Norwegian birth cohort recruited between 2001 and 2007 and followed to September 2016. SETTING: Norwegian population. PARTICIPANTS: Children carrying the HLA genotype DR4-DQ8/DR3-DQ2 conferring increased risk of coeliac disease...
February 13, 2019: BMJ: British Medical Journal
Gislaine Fongaro, Aline Viancelli, Deyse A Dos Reis, Aníbal F Santiago, Marta Hernández, Willian Michellon, Maria Célia da Silva Lanna, Helen Treichel, David Rodríguez-Lázaro
Although the effects of heavy metals on the behavior, including infectivity, of bacteria have been studied, little information is available about their effects on enteric viruses. We report an investigation of effects on the biosynthesis of human adenoviruses (HAdV) and hepatitis A (HAV) of waters contaminated with mineral waste following an environmental disaster in Mariana City, Minas Gerais State, Brazil. The study area was affected on November 5, 2015, by 60 million m3 of mud (containing very high concentrations of iron salts) from a mining reservoir (Fundão), reaching the Gualaxo do Norte River (sites evaluated in this study), the "Rio Doce" River and finally the Atlantic Ocean...
February 12, 2019: Food and Environmental Virology
Flora De Conto, Francesca Conversano, Maria Cristina Medici, Francesca Ferraglia, Federica Pinardi, Maria Cristina Arcangeletti, Carlo Chezzi, Adriana Calderaro
Acute respiratory tract infections (ARTIs) are among the leading causes of morbidity and mortality in children. The viral etiology of ARTIs was investigated over 3 years (October 2012-September 2015) in 2575 children in Parma, Italy, using indirect immunofluorescent staining of respiratory samples for viral antigens, cell culture, and molecular assays. Respiratory viruses were detected in 1299 cases (50.44%); 1037 (79.83%) were single infections and 262 (20.17%) mixed infections. The highest infection incidence was in children aged >6 months to ≤3 years (57...
January 17, 2019: Diagnostic Microbiology and Infectious Disease
Gurdeep Singh, Ashrafali M Ismail, Jeong Yoon Lee, Mirja Ramke, Ji Sun Lee, David W Dyer, Donald Seto, Jaya Rajaiya, James Chodosh
Adenovirus E1A is the first viral protein expressed during infection. E1A controls critical aspects of downstream viral gene expression and cell cycle deregulation, and its function is thought to be highly conserved among adenoviruses. Various bioinformatics analyses of E1A from 38 human adenoviruses of species D (HAdV-D), including likelihood clade model partitioning, provided highly significant evidence of divergence of HAdV-Ds into two distinct groups for the conserved region 3 (CR3), present only in the E1A 13S isoform...
February 8, 2019: Viruses
Lisa Koodie, Matthew G Robertson, Malavika Chandrashekar, George Ruth, Michele Dunning, Richard W Bianco, Julia Davydova
Oncolytic adenoviruses (Ad) are promising tools for cancer therapeutics. Most Ad-based therapies utilize species C serotypes, with Adenovirus type 5 (Ad5) most commonly employed. Prior clinical trials demonstrated low efficiency of oncolytic Ad5 vectors, mainly due to the absence of Ad5 primary receptor (Coxsackie and Adenovirus Receptor, CAR) on cancer cells. Engineering serotype chimeric vectors (Ad5/3) to utilize Adenovirus type 3 (Ad3) receptors has greatly improved their oncolytic potential. Clinical translation of these infectivity-enhanced vectors has been challenging due to a lack of replication permissive animal models...
February 8, 2019: Cancers
Martin A Prusinkiewicz, Joe S Mymryk
Viruses are obligate intracellular parasites that alter many cellular processes to create an environment optimal for viral replication. Reprogramming of cellular metabolism is an important, yet underappreciated feature of many viral infections, as this ensures that the energy and substrates required for viral replication are available in abundance. Human adenovirus (HAdV), which is the focus of this review, is a small DNA tumor virus that reprograms cellular metabolism in a variety of ways. It is well known that HAdV infection increases glucose uptake and fermentation to lactate in a manner resembling the Warburg effect observed in many cancer cells...
February 8, 2019: Viruses
Robert M Hoffman, Kenji Miki, Waddah Al-Refaie, Mingxu Xu, Yuying Tan
Recombinant methioninase (rMETase) derived from Pseudomonas putida targets the elevated methionine (MET) requirement of cancer cells (methionine dependence) and has shown efficacy against a variety of cancer types in mouse models. To enhance the efficacy of rMETase, we constructed the pLGFP-METSN retrovirus encoding the P. putida methioninase (METase) gene fused with the green fluorescent protein (GFP) gene. pLGFP-METSN or control vector pLGFPSN was introduced into the human lung cancer cell line H460. The retrovirus-mediated METase gene transfer decreased the intracellular MET level of the cancer cells and consequently enhanced the efficacy of treatment with the rMETase protein...
2019: Methods in Molecular Biology
I-Cheng Mark Chen, Jin Phang Loh, Cheryl X P Chuah, Qiu Han Christine Gao, Yinxiaohe Sun, Sock Hoon Ng, Wee-Hong Victor Koh, Ee Hui Goh, Xiahong Zhao, Paul Anantharajah Tambyah, Alex R Cook, Jeremiah Chng, Junxiong Pang, Boon-Huan Tan, Vernon J Lee
Background: Few studies have studied relative cross-protection between different groups of viruses through studies of sequential infections in humans. We aimed to investigate presence of short-lived relative cross-protection between specific prior viral infections and subsequent febrile respiratory illness (FRI). Methods: Basic Military Training (BMT) intakes between December 2009 and December 2014 were recruited, with the first FRI as the entry point. ResPlex II assays and real-time PCR assays were used to detect viral pathogens in the samples collected, followed by survival analyses to determine if infection with particular viruses conferred short-lived relative cross-protection against FRI...
February 5, 2019: Journal of Infectious Diseases
Lucas Ariel Totaro Garcia, Laurita Boff, Célia Regina Monte Barardi, Markus Nagl
Millions of people use contaminated water sources for direct consumption. Chlorine is the most widely disinfection product but can produce toxic by-products. In this context, natural and synthetic compounds can be an alternative to water disinfection. Therefore, the aim of this study was to assess the inactivation of human adenovirus by N-chlorotaurine (NCT), bromamine-T (BAT) and Grape seed extract (GSE) in water. Distilled water artificially contaminated with recombinant human adenovirus type 5 (rAdV-GFP) was treated with different concentrations of each compound for up to 120 min, and viral infectivity was assessed by fluorescence microscopy...
February 4, 2019: Food and Environmental Virology
Subrat Khanal, Pranita Ghimire, Amit S Dhamoon
We would like to thank Dr. Atkinson for his comments on our review article on the manifestations of adenoviral infections in humans. [...].
February 2, 2019: Biomedicines
Guijun Sun, Yang Yang, Xiaoyan Lu, Qing Liu, Shengrong Wu, Jiajia Jin, Zijiao Huang, Xiangyi He
OBJECTIVE: The aim of this study was to construct the periodontal ligament cells overexpressing Human telomerase reverse transcriptase(hTERT) mediated by adenovirus vectors and lentivirus, and compare the osteogenic and proliferative abilities of the two cell lines, in order to establish an efficient and stable cell model that will be more appropriate for applying to the periodontal ligament regeneration. METHODS: After the construction of the recombinant adenovirus plasmid pAd-pshuttle-cmv-hTERT, then human periodontal ligament cells were infected by the successfully packaged adenovirus and lentivirus particles, by which the two periodontal ligament cell lines were established...
February 1, 2019: Human Gene Therapy Methods
Rory Henry Macgregor Price, Catriona Graham, Sandeep Ramalingam
Numerous viruses can cause upper respiratory tract infections. They often precede serious lower respiratory tract infections. Each virus has a seasonal pattern, with peaks in activity in different seasons. We examined the effects of daily local meteorological data (temperature, relative humidity, "humidity-range" and dew point) from Edinburgh, Scotland on the seasonal variations in viral transmission. We identified the seasonality of rhinovirus, adenovirus, influenza A and B viruses, human parainfluenza viruses 1-3 (HPIV), respiratory syncytial virus (RSV) and human metapneumovirus (HMPV) from the 52060 respiratory samples tested between 2009 and 2015 and then confirmed the same by a generalised linear model...
January 30, 2019: Scientific Reports
Maheen Z Abidi, Parameswaran Hari, Min Chen, Soyoung Kim, Minoo Battiwala, Parastoo Bahrami Dahi, Miguel Angel Diaz, Robert Peter Gale, Siddhartha Ganguly, Usama Gergis, Jaime Green, Gerhard Hildebrandt, Joshua A Hill, Krishna Komanduri, Hillard Lazarus, David Marks, Taiga Nishihori, Richard Olsson, Sachiko Seo, Celalettin Ustun, Jean Yared, Dwight Yin, John Wingard, Baldeep Mona Wirk, Jeffrey Auletta, Caroline Lindemans, Marcie Riches
Limited data exist on characteristics of central nervous system viruses (CNS-V) in allogeneic hematopoietic stem cell transplant (HCT) recipients. Between 2007 and 2015, the Center for International Blood and Marrow Transplant Research (CIBMTR) received information on 27,532 patients undergoing HCT. Of these, centers reported 165 HCT recipients with CNS-V detected in cerebrospinal fluid within 6 months after HCT. CNS viruses predominantly included human herpes virus 6 (HHV-6) (73%), followed by Epstein-Barr Virus (10%), cytomegalovirus (3%), varicella zoster virus (3%), herpes simplex virus (3%) and Adenovirus (3%)...
January 29, 2019: Bone Marrow Transplantation
Juan Yu, Hong Li, Nan Nan Lu, You Ju Lei, Shuang Ying Jiang, Sheng Cang Zhao, Hua Xiang Rao
No abstract text is available yet for this article.
January 2019: Biomedical and Environmental Sciences: BES
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