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Oral SCC

En-Wei Hsing, Shine-Gwo Shiah, Hsuan-Yu Peng, Ya-Wen Chen, Chih-Pin Chuu, Jenn-Ren Hsiao, Ping-Chiang Lyu, Jang-Yang Chang
Distant metastasis leads oral cancer patients into a poor survival rate and a high recurrence stage. During tumor progression, dysregulated microRNAs (miRNAs) have been reported to involve tumor initiation and modulate oral cancer malignancy. MiR-450a was significantly upregulated in oral squamous cell carcinoma (OSCC) patients without functional reports. This study was attempted to uncover the molecular mechanism of novel miR-450a in OSCC. Mir-450a expression was examined by quantitative RT-PCR, both in OSCC cell lines and patients...
2019: PloS One
Joon Koh, Zoya B Kurago
Proliferative verrucous leukoplakia (PVL) is a premalignant condition of the oral mucosa with > 70% chance of progression to squamous cell carcinoma (SCC), while lacking the common risks and behavior seen in non-PVL oral squamous carcinogenesis. PVL follows a multi-stage slow, relentless and usually multifocal expansion of surface epithelial thickening that over time takes on a verrucous architecture, eventually leading to verrucous carcinoma and/or dysplasia followed by "conventional" SCC, a process that takes years and is notoriously difficult to manage...
March 19, 2019: Head and Neck Pathology
Cheng-Kun He, Ya-Wen Chen, Ssu-Han Wang, Chia-Hsien Hsu
Studies on cellular heterogeneity have emerged as a powerful approach for developing new strategies to treat diseases including cancer. However, it is difficult to set up an in vitro co-culture experiment to study the interaction of individual live cells. In this paper, we report a hydrodynamic shuttling chip (HSC) which can deterministically capture single cells into microfluidic chambers to set up multiple single-cell co-culture experiments in which individual live cells can be microscopically observed. Using this chip device, we demonstrated a triple single-cell culture of oral squamous cell carcinoma and lymphatic endothelial cells to observe the effect of cell-cell interaction on the cell motility...
March 19, 2019: Lab on a Chip
Hikaru Nakashima, Ryoji Yoshida, Akiyuki Hirosue, Kenta Kawahara, Junki Sakata, Hidetaka Arita, Tatsuro Yamamoto, Ryo Toya, Ryuji Murakami, Akimitsu Hiraki, Masanori Shinohara, Takaaki Ito, Yoshikazu Kuwahara, Hideki Nakayama
MicroRNAs are a class of small, endogenous, noncoding 18- to 24-nucleotide-long RNAs that can regulate multiple processes related to cancer progression. However, their clinical value in patients with oral squamous cell carcinoma has not yet been fully explored. Therefore, the aim of this study was to investigate the clinical significance of circulating microRNAs in oral squamous cell carcinoma patients. The expression levels of circulating miR-1246 and miR-1290 in healthy volunteers and oral squamous cell carcinoma patients were examined by quantitative real-time polymerase chain reaction...
March 2019: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
Sook-Bin Woo
Leukoplakia and erythroplakia are two entities under the moniker of "oral potentially malignant disorders" that are highly associated with the presence of oral epithelial dysplasia (OED) at first biopsy, while lesions of submucous fibrosis develop OED after being present for years. Importantly, traumatic/frictional keratoses are often mistaken clinically for leukoplakia and it is important for the pathologist to recognize and report them as such. The features of OED have been well-described and other architectural features will be discussed here, in particular verrucous and papillary architecture, bulky epithelial proliferation and epithelial atrophy...
March 18, 2019: Head and Neck Pathology
Justine Moe, Jonathan B McHugh, Aaron M Udager, Thomas M Braun, Joseph I Helman, Brent B Ward
PURPOSE: Depth of invasion (DOI) is one predictor of nodal metastasis in oral cavity squamous cell carcinoma (OCSCC) and can facilitate the decision to complete an elective neck dissection (END) in early-stage disease with a clinically negative neck. The purpose of this study was to investigate the accuracy of DOI in intraoperative frozen specimens for T1N0 oral OCSCC. MATERIALS AND METHODS: To compare the accuracy of DOI in frozen versus permanent specimens, we completed a prospective, blinded study of 30 patients with cT1N0 OCSCC who presented between October 2016 and December 2017...
February 20, 2019: Journal of Oral and Maxillofacial Surgery
Nuwan Dharmawardana, Eng Hooi Ooi, Charmaine Woods, Damian Hussey
Circulating microRNAs have been described as head and neck cancer biomarkers in multiple anatomical subsites including the oral cavity, nasopharynx, larynx, salivary glands and the skin. While there is an expanding volume of published literature showing the significance of individual or panels of microRNAs, the clinical validation of candidate biomarkers is lacking. The various methods used to collect, store, process and interpret these microRNAs are likely introducing bias and contributing to the inconsistent results...
March 14, 2019: Clinical & Experimental Metastasis
H Aizawa, S Yamada, H Sakai, H Otakiri, D Akita, T Gibo, H Kurita
Nuclear protein of the testis (NUT) midline carcinomas (NMC) are malignant epithelial tumours that have chromosomal rearrangements of the gene encoding NUT at 15q14. NMC is typically an aggressive fatal cancer, clinically overlaps with other carcinomas, and differential diagnosis is difficult. The purpose of this study was to investigate NMC in poorly differentiated oral squamous cell carcinoma (OSCC) with a retrospective analysis based on anti-C52B1 immunohistochemical staining. An anti-C52B1 antibody was used for immunohistochemical staining in all 27 primary tumours, and the prevalence and pathological features of NMC in the oral cavity were examined...
March 12, 2019: International Journal of Oral and Maxillofacial Surgery
Vui King Vincent-Chong, Hendrik DeJong, Kristopher Attwood, Pamela A Hershberger, Mukund Seshadri
The anticancer activity of 1,25-dihydroxyvitamin D3 (1,25(OH)2 D3 or calcitriol) has been widely reported in preclinical models. However, systematic investigation into the chemopreventive potential of calcitriol against the spectrum of oral carcinogenesis has not been performed. To address this gap in knowledge, we conducted a preclinical prevention trial of calcitriol in the 4-nitroquinoline-1-oxide (4NQO) oral carcinogenesis model. C57BL/6 mice were exposed to the carcinogen 4NQO in drinking water for 16 weeks and randomized to control (4NQO only) or calcitriol arms...
March 12, 2019: Neoplasia: An International Journal for Oncology Research
Qi Zhang, Sang Beom Lee, Xu Chen, Morgan Stevenson, Jing Pan, Donghai Xiong, Yu Zhou, Mark Miller, Ronald A Lubet, Yian Wang, Shama Mirza, Ming You
Bexarotene has exhibited inhibitory effects in preclinical models of lung, mammary gland tumorigenesis and in phase I/II trials of non-small cell lung cancer. The major side effects of bexarotene when administered orally were hypertriglyceridemia and hypercholesterolemia. In our previous study we demonstrated that aerosolized bexarotene through nasal inhalation exhibited potent chemopreventive activity in a lung adenoma preclinical model without the hypertriglyceridemia. To facilitate its future clinical translation, we modified the formula of the aerosolized bexarotene with a clinically relevant solvent system...
March 15, 2019: Nano Letters
Bingxia Shi, Chao Ma, Guolin Liu, Yanjun Guo
Background: LIM kinase 1 (LIMK1) expression levels are closely associated with microRNA (miRNA) processing. Higher levels of LIMK1 are reported during the progression of many cancers. Our study explored the interaction between LIMK1 and miR-106a in oral squamous cell carcinoma (OSCC). Methods: Quantitative RT-PCR was performed to detect the levels of LIMK1 and miR-106a in OSCC tissues and cell lines. The rates of cell proliferation and epithelial-mesenchymal transition (EMT) were assessed to determine the biological functions of miR-106a and LIMK1 in OSCC cells...
2019: Cellular & Molecular Biology Letters
Cheng-Mei Yang, Hao-Sheng Chang, Hung-Chih Chen, Jyun-Jie You, Huei-Han Liou, Su-Chen Ting, Luo-Ping Ger, Sung-Chou Li, Kuo-Wang Tsai
C6orf141 (Chromosome 6 open reading frame 141) is a novel gene, and its role in oral cancer progression remains unclear. C6orf141 expression in oral squamous cell carcinoma (OSCC) and adjacent normal tissues from 428 patients was examined through immunohistochemistry (IHC). Our results revealed that C6orf141 expression was significantly reduced in OSCC compared with adjacent normal tissues. Low C6orf141 expression was significantly associated with a poor American Joint Committee on Cancer pathological stage (P < 0...
March 14, 2019: Scientific Reports
Max Grayson, Ashley Furr, Shivani Ruparel
Considerable gap in knowledge exists about the mechanisms by which oral tumors regulate peripheral sensory fibers to produce pain and altered sensations. To address this gap, we used a murine model of oral squamous cell carcinoma (OSCC) of the tongue to investigate changes in response properties of trigeminal afferent neurons. Using this model, we developed an ex vivo method for single neuron recordings of the lingual nerve from isolated tongue tissue. Our data demonstrated that the tongue tumor produced increased spontaneous firing of lingual fibers compared to control as well as produced mechanical hypersensitivity and reduced von Frey thresholds of C- and A-slow-high-threshold mechanoreceptors (HTMR) fibers but had no effect on C-LTMR, A-slow-LTMR and A-fast lingual fibers...
March 14, 2019: Scientific Reports
Wei-Ting Ku, Jiun-Jia Tung, Tony Jer-Fu Lee, Kuo-Chu Lai
Oroxylin A (Oro-A), the main bioactive flavonoid extracted from Scutellaria radix , has been reported to inhibit migration in various human cancer cell models. In this study, we further explored the anti-migration effects of Oro-A on oral squamous cell carcinoma (OSCC) cells and investigated the underlying mechanisms. A 24-h (short-term) exposure of OSCC cells to non-cytotoxic concentrations (5⁻20 μM) of Oro-A significantly suppressed cell migration according to a wound-healing assay. Furthermore, a 30-day exposure (long-term) to Oro-A (20 μM), which did not exhibit a cytotoxic effect on OSCC cells, significantly suppressed cell migration more than short-term Oro-A exposure...
March 12, 2019: Cancers
Dan P Zandberg, Luke J Tallon, Sushma Nagaraj, Lisa K Sadzewicz, Yuji Zhang, Maxwell B Strome, Xuechu E Zhao, Kranthi Vavikolanu, Xiaoyu Zhang, John C Papadimitriou, Fleesie A Hubbard, Søren M Bentzen, Scott E Strome, Claire M Fraser
BACKGROUND: We sought to evaluate intratumor heterogeneity in squamous cell carcinoma of the oral cavity (OCC) and specifically determine the effect of physical separation and histologic differentiation within the same tumor. METHODS: We performed whole exome sequencing on five biopsy sites-two from well-differentiated, two from poorly differentiated regions, and one from normal parenchyma-from five primary OCC specimens. RESULTS: We found high levels of intratumor heterogeneity and, in four primary tumors, identified only 0 to 2 identical mutations in all subsites...
March 14, 2019: Head & Neck
Nazish Fatima, Sidra Mohiuddin, Salim Hosein, Mervyn Mervyn
BACKGROUND: This study was conducted to determine the age, gender and pattern of associated chewing habits among cases of oral squamous cell carcinoma (OSCC) presenting with, (OSMFCa) and without, (Ca-OSMF) pre-existing clinical oral submucous fibrosis in Karachi, Pakistan. METHODS: A cross sectional, multi centric study of (n=1009) cases with histologically confirmed oral squamous cell carcinoma seen at both public, private tertiary care hospitals and clinics over a period of eight years...
January 2019: Journal of Ayub Medical College, Abbottabad: JAMC
Ponnusamy Subramani Gayathri, K Saraswathi Gopal, B Gowtham Harsha Vardhan, C Krithika, Praveena Raman
Oral squamous cell carcinoma is a leading cause of mortality due to late diagnosis in India and most other developing countries. Buccal squamous cell carcinoma is almost always preceded by premalignant conditions that include leukoplakia, erythroplakia, oral lichen planus, and submucous fibrosis of the oral cavity. Hence, these patients warrant regular screening by oral health care professionals and proper monitoring for any dysplastic changes. Implementing social awareness about early signs and symptoms, as well as education on self-oral screening methods so as to avoid the risk of late presentation of oral squamous cell carcinoma, should be made mandatory for such individuals to prevent further complications...
December 31, 2018: Curēus
Jianbo Shi, Xin Bao, Zengying Liu, Zhiyuan Zhang, Wantao Chen, Qin Xu
Although serum microRNAs (miRNAs) are currently being considered as promising noninvasive biomarkers for cancers, their role in the prognosis of oral squamous cell carcinoma (OSCC) has not been elucidated. Here we aimed to identify serum miRNA biomarkers that could be used as prognosis predictors of OSCC. Methods : A cohort of 260 serum miRNA samples was assessed in a three-step approach that included a screening stage, a training stage, and a testing stage. The correlation between prognosis of OSCC and the miRNAs expression was comprehensively analyzed...
2019: Theranostics
Jing Dai, Jing-Wen Mu, Hong Mu
The present study aimed to investigate whether the long non-coding RNA (lncRNA) colorectal neoplasia differentially expressed (CRNDE) can promote the migration and invasion of human oral squamous cell carcinoma (OSCC) cells via the regulation of epithelial-mesenchymal transition (EMT). CAL-27 and SCC-15 cells were classified into a control group, a small interfering negative control (si-NC) group (cells transfected with control siRNA) and an si-CRNDE group (cells transfected with CRNDE siRNA). The expression of CRNDE in OSCC tissues and cell lines was detected by in situ hybridization (ISH) and reverse transcription-quantitative polymerase chain reaction...
March 2019: Oncology Letters
Hui Xu, Jialong Jiang, Jingjun Zhang, Liang Cheng, Song Pan, Yuanhai Li
Several studies have shown that microRNA-375 (miR-375) is frequently downregulated in several types of human cancer including gastric cancer, colorectal cancer and oral squamous cell carcinoma. However, the role of miR-375 in human esophageal cancer remains unknown. In the current study, the expression level of miR-375 was analyzed in 43 esophageal squamous cell carcinoma (ESCC) tissue and matched adjacent normal tissue samples from patients with ESCC by reverse transcription-quantitative polymerase chain reaction...
March 2019: Experimental and Therapeutic Medicine
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