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Kalyana Babu B, Mary Rani K L, Sarika Sahu, R K Mathur, Naveen Kumar P, Ravichandran G, Anitha P, Bhagya H P
The availability of large expressed sequence tag (EST) and whole genome databases of oil palm enabled the development of a data base of microsatellite markers. For this purpose, an EST database consisting of 40,979 EST sequences spanning 27 Mb and a chromosome-wise whole genome databases were downloaded. A total of 3,950 primer pairs were identified and developed from EST sequences. The tri and tetra nucleotide repeat motifs were most prevalent (each 24.75%) followed by di-nucleotide repeat motifs. Whole genome-wide analysis found a total of 245,654 SSR repeats across the 16 chromosomes of oil palm, of which 38,717 were compound microsatellite repeats...
February 13, 2019: Scientific Reports
Koen Ameloot, Marcelo B Bastos, Joost Daemen, Jan Schreuder, Eric Boersma, Felix Zijlstra, Nicolas M Van Mieghem
AIMS: To establish the value of new generation mechanical circulatory support (MCS) devices such as heartmate PHP (Abott), Impella CP (Abiomed) and PulseCath iVAC2L (Terumo). METHODS AND RESULTS: we retrospectively analyzed all consecutive elective high-risk PCI procedures performed in the Erasmus Medical Center (2011-2018) thereby comparing MCS protected and unprotected patients. The primary end-point was a composite of procedure related adverse events including death (<24 hours), cardiac arrest, need for vasopressors, rescue MCS, endotracheal intubation and limb ischemia with need for surgery...
February 12, 2019: EuroIntervention
Maria P Yavropoulou, Efstathios Chronopoulos, George Trovas, Emmanouil Avramidis, Francesca Marta Elli, Giovanna Mantovani, Pantelis Zebekakis, John G Yovos
Pseudohypoparathyroidism (PHP) is a heterogeneous group of rare endocrine disorders characterised by normal renal function and renal resistance to the action of the parathyroid hormone. Type 1A (PHP1A), which is the most common variant, also include developmental and skeletal defects named as Albright hereditary osteodystrophy (AHO). We present two cases, a 54- and a 33-year-old male diagnosed with PHP who were referred to us for persistently high levels of serum calcitonin. AHO and multinodular goitre were present in the 54-year-old male, while the second patient was free of skeletal deformities and his thyroid gland was of normal size and without nodular appearance...
January 31, 2019: Endocrinology, Diabetes & Metabolism Case Reports
Maria João M Lima, Daniela F M Cristelo, Joana B Mourão
Background: Physiological and Operative Severity Score for the Enumeration of Mortality and Morbidity (POSSUM) is a validated instrument used to predict morbidity. The aim of our study was to evaluate the performance of the POSSUM score system on predicting perioperative frailty and quality of life (QOL) in elderly surgical patients. Patients and Methods: An observational prospective study was conducted during 3 months. POSSUM was used to determine operative morbidity risk...
January 2019: Saudi Journal of Anaesthesia
Mahalaqua Nazli Khatib, Anju Sinha, Abhay M Gaidhane, Padam Simkhada, Prakash B Behere, Deepak Saxena, Bhaskaran Unnikrishnan, Afroz Khatib, Mahjabeen Ahmed, Zahiruddin Quazi Syed
Background: Substance abuse is one of the most significant global public health issues among youths. Electronic media has become a part of day-to-day life for all. This systematic review is undertaken to comprehensively explore the effect of electronic media on substance abuse among children and adolescents. Methodology: Two review authors independently searched various electronic databases and other sources. Selection Criteria: Randomized control trials that assessed the effect of exposure of electronic media (defined as television, internet, gaming, mobile phones/phones, and radio) among participants in the age range of 5-19 years on substance abuse were included in the review...
December 2018: Indian Journal of Community Medicine
Abhay M Gaidhane, Anju Sinha, Mahalaqua Nazli Khatib, Padam Simkhada, Prakash B Behere, Deepak Saxena, Bhaskaran Unnikrishnan, Mahafroz Khatib, Mahjabeen Ahmed, Zahiruddin Quazi Syed
Context: Electronic media has become a part of day-to-day life for all, and particularly more so for children and adolescents. Exposure to electronic media may be beneficial as well as harmful. Aim: The aim of the study is to systematically synthesize existing published and non-published empirical evidence on the effect of exposure to electronic media on diet, exercise, and sexual activity. Methodology: Two reviewers independently searched online databases such as MEDLINE, CENTRAL, and EMBASE...
December 2018: Indian Journal of Community Medicine
Alec B Chapman, Kelly S Peterson, Patrick R Alba, Scott L DuVall, Olga V Patterson
INTRODUCTION: Identifying occurrences of medication side effects and adverse drug events (ADEs) is an important and challenging task because they are frequently only mentioned in clinical narrative and are not formally reported. METHODS: We developed a natural language processing (NLP) system that aims to identify mentions of symptoms and drugs in clinical notes and label the relationship between the mentions as indications or ADEs. The system leverages an existing word embeddings model with induced word clusters for dimensionality reduction...
January 16, 2019: Drug Safety: An International Journal of Medical Toxicology and Drug Experience
Bence György, Elise J Meijer, Maryna V Ivanchenko, Kelly Tenneson, Frederick Emond, Killian S Hanlon, Artur A Indzhykulian, Adrienn Volak, K Domenica Karavitaki, Panos I Tamvakologos, Mark Vezina, Vladimir K Berezovskii, Richard T Born, Maureen O'Brien, Jean-François Lafond, Yvan Arsenijevic, Margaret A Kenna, Casey A Maguire, David P Corey
Hereditary hearing loss often results from mutation of genes expressed by cochlear hair cells. Gene addition using AAV vectors has shown some efficacy in mouse models, but clinical application requires two additional advances. First, new AAV capsids must mediate efficient transgene expression in both inner and outer hair cells of the cochlea. Second, to have the best chance of clinical translation, these new vectors must also transduce hair cells in non-human primates. Here, we show that an AAV9 capsid variant, PHP...
June 14, 2019: Molecular Therapy. Methods & Clinical Development
Chao Zhou, Miao Yu, Xiaowei Tang, Xiang Wang, Xiaobin Zhang, Xiangrong Zhang, Jiu Chen
OBJECTIVE: Previous studies suggested likely mechanisms underlying the dysfunction of the default mode network (DMN) in schizophrenia. However, altered patterns of the intrinsic activity of the DMN in both deficit schizophrenia (DS) and non-deficit schizophrenia (NDS) patients, as well as the neurocognitive relationships among them, remain unknown. This study explores the resting-state characteristics of the DMN activity in both DS and NDS patients, and further investigates correlations with neurocognitive features...
March 8, 2019: Progress in Neuro-psychopharmacology & Biological Psychiatry
Brittny E Manos, Terrill D Bravender, Tondi M Harrison, Hannah L H Lange, Casey B Cottrill, Mahmoud Abdel-Rasoul, Andrea E Bonny
OBJECTIVE: To evaluate the effectiveness and tolerability of omega-3 polyunsaturated fatty acid (PUFA) supplementation for treatment of trait anxiety among adolescent females with restrictive anorexia nervosa (AN). METHOD: A pilot double-blind, placebo-controlled randomized trial of adolescent females with AN (N = 24) entering Partial Hospitalization Program (PHP) from January 2015 to February 2016. Participants were randomized to four daily PUFA (2,120 mg eicosapentaenoic acid/600 mg docosohexaenoic acid) or placebo capsules for 12 weeks...
October 26, 2018: International Journal of Eating Disorders
Sofanne J Ravensbergen, Laura B Nellums, Sally Hargreaves, Ymkje Stienstra, Jon S Friedland
BACKGROUND: Migrants may be underimmunised and at higher risk of vaccine-preventable diseases, yet there has been no comprehensive examination of what policies are currently implemented across Europe targeting child and adult migrants. We analysed vaccination policies for migrants in 32 EU/EEA countries and Switzerland. METHODS: Using framework analysis, we did a comparative analysis of national policies and guidelines pertaining to vaccination in recently arrived migrants through a systematic guideline and literature review and by approaching national experts...
October 15, 2018: Travel Medicine and Infectious Disease
Yoichiro Shinohara, Ayumu Konno, Keisuke Nitta, Yasunori Matsuzaki, Hiroyuki Yasui, Junya Suwa, Keiju Hiromura, Hirokazu Hirai
Adeno-associated virus (AAV)-PHP.B, a capsid variant of AAV serotype 9, is highly permeable to the blood-brain barrier. A major obstacle to the systemic use of AAV-PHP.B is the generation of neutralizing antibodies (NAbs); however, temporal profiles of NAb production after exposure to AAV-PHP.B, and the influence on later AAV-PHP.B administration, remains unknown. To address these, AAV-PHP.Bs expressing either GFP or mCherry by neuron-specific or astrocyte-specific promoters were intravenously administered to mice at various intervals, and brain expression was examined...
October 5, 2018: Molecular Neurobiology
Venkatesh V Nemmara, Dao Feng Xiang, A A Fedorov, E V Fedorov, Jeffrey B Bonanno, Steven C Almo, Frank M Raushel
The phosphotriesterase homology protein (PHP) from Escherichia coli is a member of a family of proteins that is related to phosphotriestrase (PTE), a bacterial enzyme from cog1735 with unusual substrate specificity toward the hydrolysis of synthetic organic phosphates and phosphonates. PHP was cloned, purified to homogeneity, and functionally characterized. The three-dimensional structure of PHP was determined at a resolution of 1.84 Å with zinc and phosphate in the active site. The protein folds as a distorted (β/α)8 -barrel and possesses a binuclear metal center in the active site...
October 30, 2018: Biochemistry
Anjali Gautam, Ajay Bhambal, Swapnil Moghe
Background: Braille is important language for reading & writing for the visually impaired. It helps them understand & visualize the world via touch. Audio aids are being used to impart health education to the visually impaired. Audio aids are useful in being time saving & can be played repeatedly to achieve desired results. Tactile models help them perceive things which they can't visualize & hence are important learning tool. Aim: To evaluate the effect of Oral Health education by Audio aids, Braille & tactile models on the Oral health status of visually impaired children of Bhopal city...
September 2018: Journal of Oral Biology and Craniofacial Research
Timothy G Carroll, Rachel Garwick, Guang Wu, Gabriel Ménard
In this Article, we outline the synthesis of B(C6 F5 )3 -coordinated mono-, di-, and trivanadocene phosphorus oxide complexes, Cp2 VOP(OB(C6 F5 )3 )Ph2 (2), (Cp2 VO)2 P(OB(C6 F5 )3 )Ph (3), and (Cp2 VO)3 P(OB(C6 F5 )3 ) (4), respectively (Cp = η5 -cyclopentadienyl). The complexes were synthesized from the known reagents, Cp2 VF and Ph2 P(O)OSiMe3 (for 2) or PhP(O)(OSiMe3 )2 (for 3) or (Me3 SiO)3 PO (for 4), via Me3 SiF elimination and in the presence of B(C6 F5 )3 . The multimetallic complexes (3 and 4) could not be synthesized without the capping B(C6 F5 )3 Lewis acid, whereas the uncapped version of 2, Cp2 VOP(O)Ph2 (1), has previously been reported by us...
September 17, 2018: Inorganic Chemistry
Robert D Dayton, Mychal S Grames, Ronald L Klein
Engineered recombinant adeno-associated virus (AAV) vectors have advanced the transduction of neurons in the CNS on an expansive, wide-scale basis since the papers first using AAV9 for this purpose. Wide-scale CNS expression is relevant to gene therapy as well as indispensable for basic studies such as disease modeling. For example, the wide-scale gene transfer approach could expedite hypothesis testing in vivo relative to the generation of germ-line transgenic mice for all of the genes of interest. Wide-scale gene transfer is more efficient in neonates than in adults, so improving gene transfer efficiency in adults is an important goal...
August 2018: Gene Therapy
Brunno L Moreira, Tasos Karakostas, Luciano S Dias
BACKGROUND: Rectus femoris transfer (RFT) is performed in children with cerebral palsy to treat stiff-knee gait. However, the results are variable in part because there is no gold standard to identify appropriate candidates. Hip flexion kinematics and kinetics are important factors in the determination of peak knee flexion during gait. This study aimed to evaluate whether a kinetic preoperative analysis of hip power can be a predictor of RFT outcomes and determine its usability for identifying appropriate candidates for the procedure...
October 2018: Journal of Pediatric Orthopedics
Opeyemi O Babatunde, Amardeep Legha, Chris Littlewood, Linda S Chesterton, Martin J Thomas, Hylton B Menz, Danielle van der Windt, Edward Roddy
OBJECTIVE: To evaluate the comparative effectiveness of current treatment options for plantar heel pain (PHP). DESIGN: Systematic review and network meta-analysis (NMA). DATA SOURCES: Medline, EMBASE, CINAHL, AMED, PEDro, Cochrane Database, Web of Science and WHO Clinical Trials Platform were searched from their inception until January 2018. STUDY SELECTION: Randomised controlled trials (RCTs) of adults with PHP investigating common treatments (ie, corticosteroid injection, non-steroidal anti-inflammatory drugs, therapeutic exercise, orthoses and/or extracorporeal shockwave therapy (ESWT)) compared with each other or a no treatment, placebo/sham control...
February 2019: British Journal of Sports Medicine
Melvin Y Rincon, Filip de Vin, Sandra I Duqué, Shelly Fripont, Stephanie A Castaldo, Jessica Bouhuijzen-Wenger, Matthew G Holt
Until recently, adeno-associated virus 9 (AAV9) was considered the AAV serotype most effective in crossing the blood-brain barrier (BBB) and transducing cells of the central nervous system (CNS), following systemic injection. However, a newly engineered capsid, AAV-PHP.B, is reported to cross the BBB at even higher efficiency. We investigated how much we could boost CNS transgene expression by using AAV-PHP.B carrying a self-complementary (sc) genome. To allow comparison, 6 weeks old C57BL/6 mice received intravenous injections of scAAV2/9-GFP or scAAV2/PHP...
April 2018: Gene Therapy
Michele Berselli, Enrico Lavezzo, Stefano Toppo
Motivation: Non-B DNA conformations play an important role in genomic rearrangements, structural three-dimensional organization and gene regulation. Many non-B DNA structures show symmetrical properties as palindromes and mirrors that can form hairpins, cruciform structures or triplexes. A comprehensive tool, capable to perform a fast genome wide search for exact and degenerate symmetrical patterns, is needed for further investigating nucleotide tracts potentially forming non-B DNA structures...
July 15, 2018: Bioinformatics
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