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cerebral ischemia AND wnt

Cai-Wei Qiu, Zong-Yao Liu, Feng-Lan Zhang, Ling Zhang, Fan Li, Shu-Yi Liu, Jian-Ying He, Zhi-Cheng Xiao
Cerebral ischemic stroke is one of the leading causes of death and disability worldwide, and the only available drug treatment is limited to a short window following the ischemic event. Gastrodin is the major bioactive constituent extracted from thetuberGastrodia elata, and is currently used to treat dizziness in the clinic. "Early" application of gastrodin (before modeling or immediately after ischemic injury) has shown antioxidative and neuroprotective effects in a transient focal brain ischemia model in rodents; however, it is not known whether the delayed administration of gastrodin after permanent focal cerebral ischemia ameliorates neural injury and increases neurogenesis...
February 1, 2019: Brain Research
Guixing Zhang, Mingyue Ge, Ziwei Han, Sheng Wang, Jiangwen Yin, Li Peng, Feng Xu, Qingtong Zhang, Zhigang Dai, Liping Xie, Yan Li, Junqiang Si, Ketao Ma
AIM: The Wnt/β-catenin signaling pathway plays an important role in ischemia-reperfusion(I/R) injury, and the transforming growth factor(TGF)-β/Smad signaling pathway participates in the neuroprotection effect induced by isoflurane(ISO) postconditioning. In this study, we aimed to explore the role of the Wnt/|[beta]|-catenin β-catenin signaling pathway in the neuroprotection effect induced by ISO postconditioning, and investigate the interaction of Wnt/β-catenin and TGF-β/Smad signaling pathway in this neuroprotection effect...
December 5, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Matthew Tai Hei Chan, Jennie Yuet Yi Wong, Anthony Ka Tsun Leung, Gang Lu, Wai Sang Poon, Alexander Yuk-Lun Lau, Wai Yee Chan, George Kwok Chu Wong
BACKGROUND: Subarachnoid hemorrhage (SAH) is fatal and detrimental to quality of life. Clinically, options for monitoring are often limited, potentially missing subtle neurological changes especially in low-grade patients. This article reviewed miRNA dysregulation in SAH and analyzed their functional and clinical relevance. METHODS: With adherence to PRISMA guideline, PubMed, EMBASE, GEO and ArrayExpress were searched comprehensively for relevant clinical and animal models...
November 24, 2018: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
Hao Zhao, Wenlong Pan, Lihua Chen, Yongchun Luo, Ruxiang Xu
Mitochondrial fragmentation drastically regulates mitochondrial homeostasis in brain illness. However, the role of mitochondrial fragmentation in cerebral ischemia-reperfusion (IR) injury remains unclear. Nur77, a regulator of mitochondrial homeostasis, is associated with heart and liver IR injury, but its effects on mitochondrial function in cerebral IR injury has not been studied intensively. The aim of our study is to explore whether cerebral IR injury is modulated by Nur77 via modification of mitochondrial homeostasis...
December 2018: Journal of Molecular Histology
Carla Letizia Busceti, Luisa Di Menna, Franca Bianchi, Federica Mastroiacovo, Paola Di Pietro, Anna Traficante, Giovanna Bozza, Christof Niehrs, Giuseppe Battaglia, Valeria Bruno, Francesco Fornai, Massimo Volpe, Speranza Rubattu, Ferdinando Nicoletti
Dickkopf-3 (Dkk3) is an atypical member of the Dkk family of Wnt inhibitors, which has been implicated in the pathophysiology of neurodegenerative disorders. However, the role of Dkk3 in mechanisms of cell degeneration and protection is unknown. We used Dkk3 knockout mice to examine how endogenous Dkk3 influences ischemic brain damage. In addition, we used primary cultures of astrocytes or mixed cultures of astrocytes and neurons to investigate the action of Dkk3 on cell damage and dissect the underlying molecular mechanisms...
2018: Frontiers in Cellular Neuroscience
Zhi Huang, Lu Lu, Tianpeng Jiang, Shuai Zhang, Yaping Shen, Zhu Zheng, Ansu Zhao, Rui Gao, Rui Li, Shi Zhou, Jing Liu
The present study aimed to determine whether an miRNA (miR)-29b inhibitor protected against cerebral ischemia/reperfusion (I/R) injury in vitro and to investigate the underlying mechanisms. As a model for induced cerebral IR injury, N2a cells were exposed to an oxygen-glucose deprivation/reoxygenation (OGD/R) environment. Using this model, it was demonstrated that miR-29b was significantly upregulated compared with cells in a normal environment. The interactions between miR-29b and myeloid cell leukemia sequence (MCL)-1 were then investigated using dual-luciferase assays, revealing a strong regulation of MCL-1 through the 3'untranslated region...
October 2018: Experimental and Therapeutic Medicine
T Li, Y-C Wan, L-J Sun, S-J Tao, P Chen, C-H Liu, K Wang, C-Y Zhou, G-Q Zhao
OBJECTIVE: Dishevelled-Axin (DIX) domain containing 1 (DIXDC1), a novel DIX domain-containing protein and a positive regulator of Wingless (Wnt) signaling, has previously been reported to play multiple roles in neurodevelopment and neurological disorders. However, whether DIXDC1 plays a role during cerebral ischemia/reperfusion injury remains unknown. In this study, we investigated the potential role of DIXDC1 in neuronal injury induced by oxygen-glucose deprivation and reoxygenation (OGD/R), an in vitro model of cerebral ischemia/reperfusion injury...
September 2018: European Review for Medical and Pharmacological Sciences
Weijun Gong, Shuyan Qie, Peiling Huang, Jianing Xi
Long noncoding RNAs (lncRNAs) have been reported to be involved in several neurological pathogenesis conditions including cerebral ischemia. In the current study, the functions of lncRNA EFNA3 on hypoxia-injured rat adrenal pheochromocytoma (PC-12) cells and the underlying molecular mechanism were studied. The expression of lncRNA EFNA3 was silenced by short hairpin RNA transfection, after which the cells were subjected with hypoxia. Cell viability, migration, invasion, and apoptosis were, respectively, determined by trypan blue staining, Transwell assay, annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) double-staining, and Western blot analysis...
January 2019: Journal of Cellular Biochemistry
Cai-Wei Qiu, Zong-Yao Liu, Kun Hou, Shu-Yi Liu, Yue-Xin Hu, Ling Zhang, Feng-Lan Zhang, Ke-Ying Lv, Qiang Kang, Wei-Yan Hu, Na Ma, Yang Jiao, Wen-Jin Bai, Zhi-Cheng Xiao
Neurogenesis correlates closely with the recovery of neural function after brain ischemia but the critical proteins and signaling pathways involved remain unclear. The phosphatase WIP1 has been shown to regulate neurogenesis in models of aging. However, it is not known if WIP1 affects neurogenesis and functional recovery after brain ischemia. To explore these questions, we performed permanent middle cerebral artery occlusion (MCAO) in mice and performed BrdU labeling, neurobehavioral testing, western blotting, and immunofluorescence staining...
November 2018: Experimental Neurology
Hao Zhao, Yongchun Luo, Lihua Chen, Zhenhai Zhang, Chunsen Shen, Yunjun Li, Ruxiang Xu
Cerebral ischemia-reperfusion injury (IRI) potentiates existing brain damage and increases mortality and morbidity via poorly understood mechanisms. The aim of our study is to investigate the role of Sirtuin 3 (Sirt3) in the development and progression of cerebral ischemia-reperfusion injury with a focus on mitochondrial fission and the Wnt/β-catenin pathway. Our data indicated that Sirt3 was downregulated in response to cerebral IRI. However, the overexpression of Sirt3 reduced the brain infarction area and repressed IRI-mediated neuron apoptosis...
September 2018: Cell Stress & Chaperones
Haixia Li, Jingtao Wang, Pengqian Wang, Yingying Zhang, Jun Liu, Yanan Yu, Bing Li, Zhong Wang
Recent evidence demonstrates that a double dose of Jasminoidin (2·JA) is more effective than Jasminoidin (JA) in cerebral ischemia therapy, but its dosage-effect mechanisms are unclear. In this study, the software GeneGo MetaCore was used to perform pathway analysis of the differentially expressed genes obtained in microarrays of mice belonging to four groups (Sham, Vehicle, JA, and 2·JA), aiming to elucidate differences in JA and 2·JA's dose-dependent pharmacological mechanism from a system's perspective...
2018: BioMed Research International
Xingmiao Chen, Binghua Zhou, Tingting Yan, Hao Wu, Jinghan Feng, Hansen Chen, Chong Gao, Tao Peng, Dan Yang, Jiangang Shen
Hypoxic/ischemic stimulation could mediate growth and differentiation of neural stem/progenitor cells (NSCs) into mature neurons but its underlying mechanisms are largely unclear. Peroxynitrite formation is considered as a crucial pathological process contributing to cerebral ischemia-reperfusion injury. In the present study, we tested the hypothesis that peroxynitrite at low concentration could function as redox signaling to promote the growth of NSCs under hypoxic/ischemic conditions. Increased NSCs proliferation was accompanied by peroxynitrite production in the rat brains with 1 h of ischemia plus 7 days of reperfusion in vivo...
March 2018: Free Radical Biology & Medicine
C Qi, J Zhang, X Chen, J Wan, J Wang, P Zhang, Y Liu
Evidence indicates that after brain injury, neurogenesis is enhanced in regions such as hippocampus, striatum, and cortex. To study the role of hypoxia-inducible factor-1 (HIF‑1α) and Wnt signaling in cerebral ischemia/hypoxia-induced proliferation of neural stem cells (NSCs), we investigated the proliferation of NSCs, expression of HIF‑1α, and activation of Wnt signaling under conditions of pathologic hypoxia in vitro. NSCs were isolated from 30-day-old Sprague-Dawley rats and subjected to 0.3% oxygen in a microaerophilic incubation system...
August 15, 2017: Cellular and Molecular Biology
Tianen Zhou, Yanran Liang, Longyuan Jiang, Tao Yu, Chaotao Zeng, Enxiang Tao
Mild hypothermia is thought to be one of the most effective therapies for cerebral ischemia/reperfusion injuries. Our previous research revealed that mild hypothermia inhibits the activation of caspase-3 and protects against oxygen glucose deprivation/reoxygenation (OGD/R)-induced injury in hippocampal neurons. However, the mechanisms behind the activation of caspase-3 remain unclear. The aims of this study were to determine whether the protective effects of mild hypothermia were exerted through the Wnt/β-catenin signaling pathway...
May 13, 2017: Biochemical and Biophysical Research Communications
Jianshui Zhang, Junfeng Zhang, Cunfang Qi, Pengbo Yang, Xinlin Chen, Yong Liu
Brain microvascular endothelial cells (BMECs) play vital roles in cerebral ischemia, during which many signal pathways mediate BMECs apoptosis. In this study, we explored the potential role of Wnt3α/β-catenin signal in BMECs apoptosis induced by ischemia. Here, we found that oxygen-glucose deprivation (OGD) could induce apoptosis of BMECs with Wnt3a mRNA expression decrease. Meanwhile, activation Wnt3a/β-catenin signal with exogenous Wnt3α protein (100 ng/ml) or Lithium Chloride (LiCl, 4 mM) decreased significantly apoptosis of BMECs induced by OGD with increasing expression of Bcl-2 in the whole cell and β-catenin in the nucleus...
August 19, 2017: Biochemical and Biophysical Research Communications
Jatin Tulsulkar, Alicia Ward, Zahoor A Shah
A gender difference in stroke is observed throughout epidemiologic studies, pathophysiology, treatment and outcomes. We investigated the neuroprotective role of hemeoxygenase (HO) enzyme, which catabolizes free heme to bilirubin, carbon monoxide and biliverdin in the female brain after permanent ischemia. We have previously reported in male mice that genetic deletion of HO1 exacerbates the brain damage after permanent ischemia, and the mechanism of neuroprotection is dependent on the HO1/Wnt pathway; however, the role of HO1/Wnt mediated neuroprotection in the female brain is yet to be investigated...
May 1, 2017: Brain Research
Yu Zhang, Long-Jian Huang, Si Shi, Shao-Feng Xu, Xiao-Liang Wang, Ying Peng
AIMS: Our previous studies showed that L-3-n-butylphthalide (L-NBP), an extract from seeds of Apium graveolens Linn (Chinese celery), improved cognitive ability in animal models of cerebral ischemia, vascular dementia, and Alzheimer's disease (AD). It is well known that cognitive deficit of AD is caused by synaptic dysfunction. In this study, we investigated the effect of L-NBP on hippocampal synaptic function in APP/PS1 AD transgenic mice and related mechanisms. METHODS: Eighteen-month-old APP/PS1 transgenic (Tg) mice were administrated 15 mg/kg L-NBP by oral gavage for 3 months...
December 2016: CNS Neuroscience & Therapeutics
Rosaliana Libro, Placido Bramanti, Emanuela Mazzon
The Wnt/β-catenin or Wnt canonical pathway controls multiple biological processes throughout development and adult life. Growing evidences have suggested that deregulation of the Wnt canonical pathway could be involved in the pathogenesis of neurodegenerative diseases. The Wnt canonical signaling is a pathway tightly regulated, which activation results in the inhibition of the Glycogen Synthase Kinase 3β (GSK-3β) function and in increased β-catenin activity, that migrates into the nucleus, activating the transcription of the Wnt target genes...
August 1, 2016: Life Sciences
Bi-Qin Zhang, Guan-Yi Zheng, Yu Han, Xiao-Dong Chen, Qiong Jiang
Aims. Ilexonin A (IA), a component of the Chinese medicine Ilex pubescens, has been shown to be neuroprotective during ischemic injury. However, the specific mechanism underlying this neuroprotective effect remains unclear. Methods. In this study, we employed a combination of immunofluorescence staining, western blotting, RT-PCR, and behavioral tests, to investigate the molecular mechanisms involved in IA regulation of neuronal proliferation and regeneration after cerebral ischemia and reperfusion in rodents...
2016: Evidence-based Complementary and Alternative Medicine: ECAM
Xin He, Yunchang Mo, Wujun Geng, Yiyi Shi, Xiuxiu Zhuang, Kunyuan Han, Qinxue Dai, Shenhui Jin, Junlu Wang
Previous studies have demonstrated that pretreatment with electroacupuncture (EA) elicits rapid tolerance to focal cerebral ischemia and that Wnt/β-catenin plays an essential role in cell survival and proliferation. In the present study, we investigated the role of Wnt/β-catenin in EA pretreatment-induced neuroprotection. Two hours after EA pretreatment, focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) for 2 h. Neuronal survival, cell apoptosis, and the Garcia neurological deficit scores were evaluated 24 h after reperfusion...
July 2016: Neurochemistry International
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