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mushroom DNA

Robert Herzog, Irina Solovyeva, Michael Bölker, Luis G Lugones, Florian Hennicke
Agrocybe aegerita is a cultivated edible mushroom in numerous countries, which also serves as a model basidiomycete to study fruiting body formation. Aiming to create an easily expandable customised molecular toolset for transformation and constitutive gene of interest expression, we first created a homologous dominant marker for transformant selection. Progeny monokaryons of the genome-sequenced dikaryon A. aegerita AAE-3 used here were identified as sensitive to the systemic fungicide carboxin. We cloned the wild-type gene encoding the iron-sulphur protein subunit of succinate dehydrogenase AaeSdi1 including its up- and downstream regions, and introduced a single-point mutation (His237 to Leu) to make it confer carboxin resistance...
February 18, 2019: Molecular Genetics and Genomics: MGG
Marcin Andrzej Kotowski, Marcin Pietras, Łukasz Łuczaj
BACKGROUND: The paper presents documentation of the traditional use of wild edible mushrooms in Mazovia (33,900 km2 ), a region of Poland. METHODS: A total of 695 semi-structured interviews were carried out among local informants in 38 localities proportionally distributed throughout the study area (one locality approximately every 30 km), asking which mushrooms they collected and how. The species utilized were identified using visual props, morphological identification of voucher specimens, and DNA barcoding...
February 12, 2019: Journal of Ethnobiology and Ethnomedicine
Luying Shan, Dazhou Wang, Yinjiao Li, Shi Zheng, Wentao Xu, Ying Shang
Tricholoma matsutake is a rare, precious, and wild edible fungus that could not be cultivated artificially until now. This situation has given way to the introduction of fake T. matsutake commodities to the mushroom market. Among the methods used to detect food adulteration, amplification of species-specific diagnostic marker is particularly important and accurate. In this study, the Pol gene is reported as a species-specific diagnostic marker to identify three T. matsutake varieties and 10 other types of edible mushrooms through qualitative and quantitative PCR...
January 28, 2019: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Xiangdong Xin, Juan Yin, Bei Zhang, Zhiyong Li, Shan Zhao, Zhongzheng Gui
The entomopathogenic mushroom Cordyceps militaris is a storehouse of various medicinal compounds and pharmacological effects. However, the high frequency of strain degeneration during subculture and preservation severely limits the large-scale production of C. militaris. DNA methylation is an important epigenomic modification involved in gene regulation. In this study, we used bisulfite sequencing for DNA methylation profiling of wild-type and mutant C. militaris. The differentially methylated regions (DMRs) of the two types were analyzed using Gene Ontology (GO) clustering and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis...
January 25, 2019: Archives of Microbiology
Masanori Tohno, Yasuhiro Tanizawa, Yoichiro Kojima, Mitsuo Sakamoto, Yasukazu Nakamura, Moriya Ohkuma, Hisami Kobayashi
A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, strain YK43T , isolated from spent mushroom substrates stored in Nagano, Japan was performed. Growth was detected at 15-45 °C, pH 5.0-8.5, and 0-10 % (w/v) NaCl. The genomic DNA G+C content of strain YK43T was 43.6 mol%. The predominant fatty acids were C16 : 0, C18 : 1 ω9c and summed feature 8. Based on 16S rRNA gene sequence analysis, the type strains of Lactobacillus acidipiscis (sequence similarity, 97...
January 10, 2019: International Journal of Systematic and Evolutionary Microbiology
Roberta Saltarelli, Francesco Palma, Anna Maria Gioacchini, Cinzia Calcabrini, Umberto Mancini, Roberta De Bellis, Vilberto Stocchi, Lucia Potenza
ETHNOPHARMACOLOGICAL RELEVANCE: Ganoderma lucidum (Curtis) P. Karst. (also known as Linghzhi and Reishi) is the most appreciated and revered medicinal mushroom across many Asian countries, but its properties have also attracted interest in Western countries. Indeed, in the West, it is now commercially available as a dietary supplement in preparations mainly made from spores, fruiting bodies and mycelia. It is employed in both nutraceutical and pharmacological formulations either for its immuno-modulating anti-inflammatory properties or as an effective adjuvant therapy in the treatment of several chronic diseases as well as in cancer treatment...
December 1, 2018: Journal of Ethnopharmacology
Fabricio Rocha Vieira, John Andrew Pecchia, Fernando Segato, Igor Polikarpov
AIMS: Characterization of the bacterial community composition and functionality and their impact on substrate biodegradation as well as mushroom yield. METHODS AND RESULTS: Bacterial diversity, composition, and functionality were accessed by DNA-derived analysis for a sugarcane straw-based substrate composted for either 5, 10 or 15 days. In addition, carbon and nitrogen losses, carbohydrates conversion as well as mushroom yields were measured for the different treatments...
November 29, 2018: Journal of Applied Microbiology
Gloria I Solano-Aguilar, Saebyeol Jang, Sukla Lakshman, Richi Gupta, Ethiopia Beshah, Masoumeh Sikaroodi, Bryan Vinyard, Aleksey Molokin, Patrick M Gillevet, Joseph F Urban
A study was designed to determine the potential prebiotic effect of dietary mushrooms on the host immune response, and intestinal microbiota composition and function. Thirty-one six-week-old pigs were fed a pig grower diet alone or supplemented with either three or six servings of freeze-dried white button (WB)-mushrooms for six weeks. Host immune response was evaluated in peripheral blood mononuclear cells (PBMC), and alveolar macrophages (AM) after stimulation with Salmonella typhymurium -Lipopolysaccharide (LPS)...
November 9, 2018: Nutrients
Ryoiti Kiyama, Yoshiyuki Furutani, Kayoko Kawaguchi, Toshio Nakanishi
Sparassis crispa (Hanabiratake) is a widely used medicinal mushroom in traditional Chinese medicine because it contains materials with pharmacological activity. Here, we report its 39.0-Mb genome, encoding 13,157 predicted genes, obtained using next-generation sequencing along with RNA-seq mapping data. A phylogenetic analysis by comparison with 25 other fungal genomes revealed that S. crispa diverged from Postia placenta, a brown-rot fungus, 94 million years ago. Several features specific to the genome were found, including the A-mating type locus with the predicted genes for HD1 and HD2 heterodomain transcription factors, the mitochondrial intermediate peptidase (MIP), and the B-mating type locus with seven potential pheromone receptor genes and three potential pheromone precursor genes...
October 30, 2018: Scientific Reports
Clare H Scott Chialvo, Brooke E White, Laura K Reed, Kelly A Dyer
Adaptive radiations provide an opportunity to examine complex evolutionary processes such as ecological specialization and speciation. While a well-resolved phylogenetic hypothesis is critical to completing such studies, the rapid rates of evolution in these groups can impede phylogenetic studies. Here we study the quinaria and testacea species groups of the immigrans-tripunctata radiation of Drosophila, which represent a recent adaptive radiation and are a developing model system for ecological genetics. We were especially interested in understanding host use evolution in these species...
October 23, 2018: Molecular Phylogenetics and Evolution
Patrick M Arnott, Himanshu Joshi, Aleksei Aksimentiev, Stefan Howorka
Lipid-anchored DNA can attach functional cargo to bilayer membranes in DNA nanotechnology, synthetic biology, and cell biology research. To optimize DNA anchoring, an understanding of DNA-membrane interactions in terms of binding strength, extent, and structural dynamics is required. Here we use experiments and molecular dynamics (MD) simulations to determine how the membrane binding of cholesterol-modified DNA depends on electrostatic and steric factors involving the lipid headgroup charge, duplexed or single-stranded DNA, and the buffer composition...
October 10, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Venkatachalam Angamuthu, M Shanmugavadivu, G Nagarajan, Bharath Kumar Velmurugan
Antrodia camphorata is an expensive mushroom that grows on the inner cavity of an endangered native tree of Taiwan namely Cinnamomum kanehirai Hayata. It is used as a traditional medicine in Taiwan and has several health benefits including free radical scavenging, anti-inflammatory, antimicrobial, hepatoprotective, neuroprotective, antidiabetic, and free radical-induced DNA damage protecting activities. Antroquinonol is a tetrahydro ubiquinone derivative found predominately in the mycelium of Antrodia camphorata, and is characterized by numerous biological and pharmacological activities...
October 19, 2018: Chemico-biological Interactions
Mouthana N Al-Habib, John Holliday, Mohammad S Aladahmy
In this study, the aqueous extracts of fruiting bodies of Psathyrella candolleana and Agaricus bisporus were assessed in vitro for their genotoxic potential. Extracts of these 2 fungi were tested with regard to their capacity to protect genetic material against DNA damage caused by the anticancer chemotherapy drug, doxorubicin. Using chromosomal aberration, micronucleus, and comet assays, the genotoxic and genoprotective potential of these 2 fungi was assessed using P. candolleana strain RM-0861 and A. bisporus strain AB-62...
2018: International Journal of Medicinal Mushrooms
Qiang Li, Qiangfeng Wang, Xin Jin, Zuqin Chen, Chuan Xiong, Ping Li, Qiaofeng Liu, Wenli Huang
Lactarius is one of the most prominent genera of mushroom-forming fungi in the world. In the present study, complete mitochondrial genomes (mitogenomes) from six Lactarius species were sequenced and assembled. The six mitogenomes were all composed of circular DNA molecules, with total lengths ranging from 38,445 bp to 60,843 bp. The GC contents, GC skews, and AT skews of the mitogenomes varied among the six species. Mitogenomic synteny analysis revealed the presence of gene rearrangements among the mitogenomes...
January 2019: International Journal of Biological Macromolecules
Idan Alyagor, Victoria Berkun, Hadas Keren-Shaul, Neta Marmor-Kollet, Eyal David, Oded Mayseless, Noa Issman-Zecharya, Ido Amit, Oren Schuldiner
Developmental neuronal remodeling is an evolutionarily conserved mechanism required for precise wiring of nervous systems. Despite its fundamental role in neurodevelopment and proposed contribution to various neuropsychiatric disorders, the underlying mechanisms are largely unknown. Here, we uncover the fine temporal transcriptional landscape of Drosophila mushroom body γ neurons undergoing stereotypical remodeling. Our data reveal rapid and dramatic changes in the transcriptional landscape during development...
October 8, 2018: Developmental Cell
Md Asaduzzaman Khan, Mousumi Tania
BACKGROUND: Cordycepin is a nucleotide analogue from Cordyceps mushrooms, which have a notable place in traditional medicine. OBJECTIVE: In this review article, we have discussed the recent findings on the molecular aspects of cordycepin interactions with its recognized cellular targets, and possible mechanisms of its anticancer activity. METHOD: We have explored databases like pubmed, google scholar, scopus and web of science for the update information on cordycepin and mechanisms of its anticancer activity, and reviewed in this study...
September 30, 2018: Current Medicinal Chemistry
Mobolaji Adeniyi, Yinka Titilawo, Anthonia Oluduro, Olu Odeyemi, Motebang Nakin, Anthony Ifeanyi Okoh
Identification of fungal species based on morphological characteristics is tedious, complex, prone to errors, and thus cannot be completely relied upon. In this study, internal transcribed spacers (ITS 1 and 4)-polymerase chain reaction was employed to amplify DNA of 19 mushroom isolates collected at Environmental Pollution Science and Technology farm, Ilesa, Southwest Nigeria. The PCR amplification of ITS1 and 4 of the mushrooms isolates yielded approximately 850 bp. Amplicons obtained were sequenced and identified using BLASTn in the NCBI...
September 20, 2018: AMB Express
Hsin-Ling Yang, Ruei-Wan Lin, Peramaiyan Rajendran, Dony Chacko Mathew, Varadharajan Thigarajan, Chuan-Chen Lee, Chih-Jung Hsu, You-Cheng Hseu
Antrodia salmonea is well known in Taiwan as a traditional Chinese medicinal fungus and has demonstrated antioxidant, anti-inflammatory, and anticancer effects. However, the anticancer activity of A. salmonea against human ovarian cancer is still elusive. Therefore, we investigated the antiovarian tumor activity of a fermented culture broth of A. salmonea and exhibits its underlying molecular mechanism. A. salmonea shows a significant effect on cell viability in human ovarian carcinoma (SKOV-3 or A2780) cell lines with an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay...
March 2019: Journal of Cellular Physiology
Andrew L Loyd, Brantlee S Richter, Michelle A Jusino, Camille Truong, Matthew E Smith, Robert A Blanchette, Jason A Smith
Species of Ganoderma , commonly called reishi (in Japan) or lingzhi (in China), have been used in traditional medicine for thousands of years, and their use has gained interest from pharmaceutical industries in recent years. Globally, the taxonomy of Ganoderma species is chaotic, and the taxon name Ganoderma lucidum has been used for most laccate (shiny) Ganoderma species. However, it is now known that G. lucidum sensu stricto has a limited native distribution in Europe and some parts of China. It is likely that differences in the quality and quantity of medicinally relevant chemicals occur among Ganoderma species...
2018: Frontiers in Microbiology
Janne Koskinen, Tomas Roslin, Tommi Nyman, Nerea Abrego, Craig Michell, Eero J Vesterinen
Fruiting bodies of fungi constitute an important resource for thousands of other taxa. The structure of these diverse assemblages has traditionally been studied with labour-intensive methods involving cultivation and morphology-based species identification, to which molecular information might offer convenient complements. To overcome challenges in DNA extraction and PCR associated with the complex chemical properties of fruiting bodies, we developed a pipeline applicable for extracting amplifiable total DNA from soft fungal samples of any size...
July 24, 2018: Molecular Ecology
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