keyword
https://read.qxmd.com/read/38537148/conformation-selection-by-atp-competitive-inhibitors-and-allosteric-communication-in-erk2
#1
JOURNAL ARTICLE
Jake W Anderson, David Vaisar, David N Jones, Laurel M Pegram, Guy P Vigers, Huifen Chen, John G Moffat, Natalie G Ahn
Activation of the extracellular signal-regulated kinase-2 (ERK2) by phosphorylation has been shown to involve changes in protein dynamics, as determined by hydrogen-deuterium exchange mass spectrometry (HDX-MS) and NMR relaxation dispersion measurements. These can be described by a global exchange between two conformational states of the active kinase, named 'L' and 'R,' where R is associated with a catalytically productive ATP-binding mode. An ATP-competitive ERK1/2 inhibitor, Vertex-11e, has properties of conformation selection for the R-state, revealing movements of the activation loop that are allosterically coupled to the kinase active site...
March 27, 2024: ELife
https://read.qxmd.com/read/38535745/the-effect-of-oral-adenosine-triphosphate-atp-supplementation-on-anaerobic-exercise-in-healthy-resistance-trained-individuals-a-systematic-review-and-meta-analysis
#2
REVIEW
Roberto González-Marenco, Ivonne Azeret Estrada-Sánchez, Martha Medina-Escobedo, Rodolfo Chim-Aké, Roberto Lugo
Adenosine triphosphate (ATP) is an energy and signaling molecule. It is synthesized endogenously and can be taken as an oral supplement. This review aimed to identify the effects of oral ATP supplementation on anaerobic exercise in healthy resistance-trained adults. A systematic review and meta-analysis were performed based on the Preferred Reporting Items of Systematic Reviews and Meta-Analysis (PRISMA) criteria. The inclusion criteria were articles published from 2000 to 2022, with anaerobic variables (maximal strength, maximum repetitions, and maximum anaerobic power) measurable in healthy adults with experience in resistance training, only randomized placebo-controlled clinical trials (RCTs), and with the acute (a single dose 30 min to 24 h before the tests) and/or chronic (>1 day) oral supplementation of ATP...
March 14, 2024: Sports
https://read.qxmd.com/read/38532054/atp-releasing-channels-and-the-ameliorative-effects-of-high-intensity-interval-training-on-diabetic-heart-a-multifaceted-analysis
#3
JOURNAL ARTICLE
Siyavash Joukar, Mohammad Amin Rajizadeh, Mohammad Abbas Bejeshk, Samaneh Sadat Alavi, Fatemeh Bagheri, Mohammad Rami, Kayvan Khoramipour
Type 2 diabetes (T2D) can cause severe cardiac complications at functional, histologic and molecular levels. These pathological complications could be mediated by ATP-releasing channels such as Panx1 and ATP receptors, in particular P2X7. The aim of our study was to investigate the effect of high-intensity interval training (HIIT) on T2D-induced cardiac complications at the functional, histopathological and molecular levels, with a particular focus on ATP-releasing channels. 48 male Wistar rats at the age of 8 weeks were randomly allocated into four groups: control (Con), Diabetes (T2D), Training (TR), and Diabetes + Training (T2D + TR)...
March 26, 2024: Scientific Reports
https://read.qxmd.com/read/38532010/serotonin-effects-on-human-ipsc-derived-neural-cell-functions-from-mitochondria-to-depression
#4
JOURNAL ARTICLE
Iseline Cardon, Sonja Grobecker, Frederike Jenne, Tatjana Jahner, Rainer Rupprecht, Vladimir M Milenkovic, Christian H Wetzel
Depression's link to serotonin dysregulation is well-known. The monoamine theory posits that depression results from impaired serotonin activity, leading to the development of antidepressants targeting serotonin levels. However, their limited efficacy suggests a more complex cause. Recent studies highlight mitochondria as key players in depression's pathophysiology. Mounting evidence indicates that mitochondrial dysfunction significantly correlates with major depressive disorder (MDD), underscoring its pivotal role in depression...
March 26, 2024: Molecular Psychiatry
https://read.qxmd.com/read/38531790/mechanical-stretch-induced-atp-release-from-osteocytes-promotes-osteogenesis-of-bone-marrow-mesenchymal-stem-cells
#5
JOURNAL ARTICLE
Yiwen Ru, Hong Gu, Lian Sun, WeiBing Zhang, Lin Wang
BACKGROUND: Mandibular distraction osteogenesis (MDO) is a highly effective method for bone regeneration, commonly employed in treating craniofacial defects and deformities. Osteocytes sense mechanical forces in the pericellular space, relay external stimuli to biochemical changes, and send signals to other effector cells, including bone marrow mesenchymal stem cells (BM-MSCs), to regulate bone resorption and formation. Piezo1 potentially affects the secretion signal molecules of bone cells under mechanical stretch...
March 2024: Discovery Medicine
https://read.qxmd.com/read/38527965/electron-transport-chain-mediated-selective-growth-of-gold-nanocrystals-in-the-intermembrane-space-of-live-microbial-cells
#6
JOURNAL ARTICLE
Yonghee Shin, Youngwook Lim, A Ram Lee, Luke P Lee, Dongchoul Kim, Mi-La Cho, Taewook Kang
Hybridization of microbial cells with inorganic nanoparticles that could dramatically improve cellular functions such as electron transfer has been realized by the random attachment or stochastic entry of the nanoparticles. Clearly, the selective growth of inorganic nanoparticles on target functional organelles is ideal for such hybridization. Here, we report the selective growth of gold nanocrystals in the intermembrane space (IMS) of Escherichia coli by exploiting the electron transport chain (ETC). We systematically show that gold ions are permeated through porins in the outer membrane of E...
March 25, 2024: ACS Nano
https://read.qxmd.com/read/38525008/il-12-overexpressed-nanoparticles-suppress-the-proliferation-of-melanoma-through-inducing-icd-and-activating-dc-cd8-t-and-cd4-t-cells
#7
JOURNAL ARTICLE
Huan-Huan Shen, Jie-Fei Peng, Ran-Ran Wang, Ping-Yu Wang, Jia-Xiang Zhang, Hong-Fang Sun, Yan Liang, Yan-Mei Li, Jiang-Nan Xue, You-Jie Li, Guang-Bin Sun, Shu-Yang Xie
PURPOSE: The drug resistance and low response rates of immunotherapy limit its application. This study aimed to construct a new nanoparticle (CaCO3 -polydopamine-polyethylenimine, CPP) to effectively deliver interleukin-12 (IL-12) and suppress cancer progress through immunotherapy. METHODS: The size distribution of CPP and its zeta potential were measured using a Malvern Zetasizer Nano-ZS90. The morphology and electrophoresis tentative delay of CPP were analyzed using a JEM-1400 transmission electron microscope and an ultraviolet spectrophotometer, respectively...
2024: International Journal of Nanomedicine
https://read.qxmd.com/read/38522485/activation-of-mitok-atp-channels-induces-penile-vasodilation-and-inhibits-mitochondrial-respiration-and-ros-production-role-of-no
#8
JOURNAL ARTICLE
Alfonso Gómez Del Val, Cristina Contreras, Mercedes Muñoz, Javier Sáenz-Medina, Marian Mohamed, Luis Rivera, Ana Sánchez, Dolores Prieto
OBJECTIVE: Mitochondrial ATP-sensitive K+ (mitoKATP ) channels are involved in neuronal and cardiac protection from ischemia and oxidative stress. Penile erection is a neurovascular event mediated by relaxation of the erectile tissue via nitric oxide (NO) released from nerves and endothelium. In the present study, we investigated whether mitoKATP channels play a role in the control of penile vascular tone and mitochondrial dynamics, and the involvement of NO. METHODS: The effect of the selective mitoKATP activator BMS191095 was examined on vascular tone, on mitochondrial bioenergetics by real-time measurements with Agilent Seahorse and on ROS production by Mitosox fluorescence in freshly isolated microarteries...
March 22, 2024: Free Radical Biology & Medicine
https://read.qxmd.com/read/38521349/autorepression-of-yeast-hsp70-co-chaperones-by-intramolecular-interactions-involving-their-j-domains
#9
JOURNAL ARTICLE
Mathieu E Rebeaud, Satyam Tiwari, Bruno Fauvet, Adelaïde Mohr, Paolo De Los Rios, Pierre Goloubinoff
The Hsp70 chaperones control protein homeostasis in all ATP-containing cellular compartments. J-domain proteins (JDPs) co-evolved with Hsp70s to trigger ATP-hydrolysis and catalytically upload various substrate polypeptides in need to be structurally modified by the chaperone. Here, we measured the protein disaggregation and refolding activities of the main yeast cytosolic Hsp70, Ssa1, in the presence of its most abundant JDPs, Sis1 and Ydj1, and two swap mutants, in which the J-domains have been interchanged...
March 21, 2024: Cell Stress & Chaperones
https://read.qxmd.com/read/38521184/a-practical-and-robust-method-to-evaluate-metabolic-fluxes-in-primary-pancreatic-islets
#10
JOURNAL ARTICLE
Debora S Rocha, Antonio C Manucci, Alexandre Bruni-Cardoso, Alicia J Kowaltowski, Eloisa A Vilas-Boas
OBJECTIVE: Evaluation of mitochondrial oxygen consumption and ATP production is important to investigate pancreatic islet pathophysiology. Most studies use cell lines due to difficulties in measuring primary islet respiration, which requires specific equipment and consumables, is expensive and poorly reproducible. Our aim was to establish a practical method to assess primary islet metabolic fluxes using standard commercial consumables. METHODS: Pancreatic islets were isolated from mice/rats, dispersed with trypsin, and adhered to pre-coated standard Seahorse or Resipher microplates...
March 21, 2024: Molecular Metabolism
https://read.qxmd.com/read/38520710/indicaxanthin-prevents-eryptosis-induced-by-cigarette-smoke-extract-by-interfering-with-active-fas-mediated-signaling
#11
JOURNAL ARTICLE
Ignazio Restivo, Ilenia Concetta Giardina, Rosario Barone, Antonio Cilla, Stefano Burgio, Mario Allegra, Luisa Tesoriere, Alessandro Attanzio
A physiological mechanism of programmed cell death called eryptosis occurs in aged or damaged red blood cells (RBCs). Dysregulated eryptosis contributes to abnormal microcirculation and prothrombotic risk. Cigarette smoke extract (CSE) induces a p38 MAPK-initiated, Fas-mediated eryptosis, activating the death-inducing signaling complex (DISC). Indicaxanthin (Ind) from cactus pear fruits, is a bioavailable dietary phytochemical in humans and it is able to incorporate into RBCs enhancing their defense against numerous stimuli...
March 23, 2024: BioFactors
https://read.qxmd.com/read/38518562/on-chip-bioluminescence-biosensor-for-the-detection-of-microbial-surface-contamination
#12
JOURNAL ARTICLE
Reza Abbasi, Meruyert Imanbekova, Sebastian Wachsmann-Hogiu
Detection of microbial pathogens is important for food safety reasons, and for monitoring sanitation in laboratory environments and health care settings. Traditional detection methods such as culture-based and nucleic acid-based methods are time-consuming, laborious, and require expensive laboratory equipment. Recently, ATP-based bioluminescence methods were developed to assess surface contamination, with commercial products available. In this study, we introduce a biosensor based on a CMOS image sensor for ATP-mediated chemiluminescence detection...
March 16, 2024: Biosensors & Bioelectronics
https://read.qxmd.com/read/38514805/one-year-trial-evaluating-the-durability-and-antimicrobial-efficacy-of-copper-in-public-transportation-systems
#13
JOURNAL ARTICLE
Teresa C Williams, Edouard Asselin, Tony Mazzulli, Tracey Woznow, Hadi Hamzeh, Davood Nahkaie, Dean Waisman, Biljana Stojkova, Richard Dixon, Elizabeth Bryce, Marthe Charles
Surfaces on transit vehicles are frequently touched and could potentially act as reservoirs for micro-organism transmission. Regular cleaning and disinfection to minimize the spread of micro-organisms is operationally challenging due to the need to keep vehicles in circulation. The application of copper (Cu) alloys to high- touch surfaces could help reduce the risk of cross-contamination, however, little is known about the durability and efficacy of engineered copper surfaces after prolonged use. Three Cu products (decal, thermal fabrication, and alloy covers) were assessed over a 12-month period...
March 21, 2024: Scientific Reports
https://read.qxmd.com/read/38513841/role-of-macrophage-bioenergetics-in-n-acetylcysteine-mediated-mitigation-of-lung-injury-and-oxidative-stress-induced-by-nitrogen-mustard
#14
JOURNAL ARTICLE
Rama Malaviya, Jaclynn Meshanni, Vasanthi R Sunil, Alessandro Venosa, Changjiang Guo, Elena V Abramova, Kinal N Vayas, Chenghui Jiang, Jessica A Cervelli, Andrew J Gow, Jeffrey D Laskin, Debra L Laskin
Nitrogen mustard (NM) is a toxic vesicant that causes acute injury to the respiratory tract. This is accompanied by an accumulation of activated macrophages in the lung and oxidative stress which have been implicated in tissue injury. In these studies, we analyzed the effects of N-acetylcysteine (NAC), an inhibitor of oxidative stress and inflammation on NM-induced lung injury, macrophage activation and bioenergetics. Treatment of rats with NAC (150 mg/kg, i.p., daily) beginning 30 min after administration of NM (0...
March 19, 2024: Toxicology and Applied Pharmacology
https://read.qxmd.com/read/38507415/protocol-for-analyzing-energy-metabolic-pathway-dependency-in-human-liver-cancer-cell-lines
#15
JOURNAL ARTICLE
Sk Ramiz Islam, Sebabrata Maity, Oishee Chakrabarti, Soumen Kanti Manna
Cellular energy metabolism analysis is complex, expensive, and indirect. We present a protocol to analyze relative contribution of metabolic pathways to ATP production by directly measuring ATP levels. We describe steps for cell counting and seeding in 96-well plate, treating with metformin, and systematic inhibition with metabolic inhibitors. We then detail procedures for a viability and ATP assay and calculating energy metabolism dependency. This high-throughput and accessible protocol works with any cell line and allows for flexible perturbation studies...
March 18, 2024: STAR protocols
https://read.qxmd.com/read/38505093/can-the-adenosine-triphosphate-atp-bioluminescence-assay-be-used-as-an-indicator-for-hospital-cleaning-a-pilot-study
#16
JOURNAL ARTICLE
Valerie Niephaus, Nina Parohl, Sabine Heiligtag, Henning Reuter, Reiner Hackler, Walter Popp
BACKGROUND: In hospital cleaning, there is currently no standard for uniform monitoring of surface cleaning, either in Germany or internationally. One possibility for monitoring is the use of so-called objective methods for checking cleaning performance (e.g. fluorescence or adenosine triphosphate (ATP) method). AIM: The aim of the study was to monitor and evaluate the implementation of the adenosine triphosphate (ATP) bioluminescence assay as a cleaning indicator in everyday hospital cleaning, in order to verify its utility and effectiveness...
2024: GMS Hygiene and Infection Control
https://read.qxmd.com/read/38504298/evaluation-of-the-efficacy-of-a-simulation-model-used-in-oral-and-maxillofacial-surgery-education
#17
JOURNAL ARTICLE
Aysegul Erten Taysi, Nuri Mert Taysi, Soner Sismanoglu
BACKGROUND: The traditional teaching methods of dental education are gradually being replaced with futuristic education methods based on the usage of educational tools such as mannequin-based simulation models and virtual reality. However, the effectiveness of mannequin-based simulation models as a learning method in the field of oral surgery remains unclear. This study aims to investigate the efficacy of training on a tooth extraction model (TEM) in view of undergraduate dental students' experience and perception of their education...
March 19, 2024: BMC Medical Education
https://read.qxmd.com/read/38503156/rhodiola-crenulata-alleviates-hypobaric-hypoxia-induced-brain-injury-by-maintaining-bbb-integrity-and-balancing-energy-metabolism-dysfunction
#18
JOURNAL ARTICLE
Ya Hou, Fuhan Fan, Na Xie, Yi Zhang, Xiaobo Wang, Xianli Meng
BACKGROUND/PURPOSE: Rhodiola crenulata (Hook. f. et Thoms.) H. Ohba (R. crenulate), a famous and characteristic Tibetan medicine, has been demonstrated to exert an outstanding brain protection role in the treatment of high-altitude hypoxia disease. However, the metabolic effects of R. crenulate on high-altitude hypoxic brain injury (HHBI) are still incompletely understood. Herein, the anti-hypoxic effect and associated mechanisms of R. crenulate were explored through both in vivo and in vitro experiments...
March 11, 2024: Phytomedicine
https://read.qxmd.com/read/38501806/real-time-measurements-of-atp-dynamics-via-ateams-in-plasmodium-falciparum-reveal-drug-class-specific-response-patterns
#19
JOURNAL ARTICLE
Eric Springer, Kim C Heimsch, Stefan Rahlfs, Katja Becker, Jude M Przyborski
Malaria tropica , caused by the parasite Plasmodium falciparum ( P. falciparum ), remains one of the greatest public health burdens for humankind. Due to its pivotal role in parasite survival, the energy metabolism of P. falciparum is an interesting target for drug design. To this end, analysis of the central metabolite adenosine triphosphate (ATP) is of great interest. So far, only cell-disruptive or intensiometric ATP assays have been available in this system, with various drawbacks for mechanistic interpretation and partly inconsistent results...
March 19, 2024: Antimicrobial Agents and Chemotherapy
https://read.qxmd.com/read/38501691/assessment-of-aspirin-and-clopidogrel-resistance-in-patients-undergoing-cardiovascular-surgery-a-single-center-cross-sectional-study
#20
JOURNAL ARTICLE
Abdullah Özer, Hüseyin Demirtaş, Sercan Tak, Başak Koçak, Eda Nur Yiğiter, Gürsel Levent Oktar, Zühre Kaya
OBJECTIVES: We aimed to investigate the antiplatelet drug resistance utilizing light transmission-lumiaggregometry (LT-LA) and platelet function analyzer-100 (PFA-100) in patients undergoing cardiovascular surgery. MATERIALS AND METHODS: The study included 60 patients diagnosed with stable coronary artery disease and peripheral vascular diseases that required surgery. The participants were divided into three groups: patients receiving Aspirin (ASA) (n=21), patients receiving Clopidogrel (CLO) (n=19), and patients receiving dual therapy (ASA+CLO) (n=20)...
March 19, 2024: Turkish Journal of Haematology: Official Journal of Turkish Society of Haematology
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