keyword
https://read.qxmd.com/read/36980938/genome-analysis-using-whole-exome-sequencing-of-non-syndromic-cleft-lip-and-or-palate-from-malagasy-trios-identifies-variants-associated-with-cilium-related-pathways-and-asian-genetic-ancestry
#21
JOURNAL ARTICLE
Zarko Manojlovic, Allyn Auslander, Yuxin Jin, Ryan J Schmidt, Yili Xu, Sharon Chang, Ruocen Song, Sue A Ingles, Alana Nunes, K C Vavra, Devin Feigelson, Sylvia Rakotoarison, Melissa DiBona, Kathy Magee, Operation Smile, Anjaramamy Ramamonjisoa, William Magee Iii
BACKGROUND: Orofacial clefts (OFCs) are common congenital disabilities that can occur as isolated non-syndromic events or as part of Mendelian syndromes. OFC risk factors vary due to differences in regional environmental exposures, genetic variants, and ethnicities. In recent years, significant progress has been made in understanding OFCs, due to advances in sequencing and genotyping technologies. Despite these advances, very little is known about the genetic interplay in the Malagasy population...
March 7, 2023: Genes
https://read.qxmd.com/read/36902838/identification-of-novel-risk-variants-of-non-syndromic-cleft-palate-by-targeted-gene-panel-sequencing
#22
JOURNAL ARTICLE
Justyna Dąbrowska, Barbara Biedziak, Agnieszka Bogdanowicz, Adrianna Mostowska
Non-syndromic cleft palate (ns-CP) has a genetically heterogeneous aetiology. Numerous studies have suggested a crucial role of rare coding variants in characterizing the unrevealed component of genetic variation in ns-CP called the "missing heritability". Therefore, this study aimed to detect low-frequency variants that are implicated in ns-CP aetiology in the Polish population. For this purpose, coding regions of 423 genes associated with orofacial cleft anomalies and/or involved with facial development were screened in 38 ns-CP patients using the next-generation sequencing technology...
March 4, 2023: Journal of Clinical Medicine
https://read.qxmd.com/read/36768749/gene-expression-profile-of-uterine-leiomyoma-from-women-exposed-to-different-air-pollution-levels-in-metropolitan-cities-of-sao-paulo-brazil
#23
JOURNAL ARTICLE
Laura Gonzalez Dos Anjos, Bruna Cristine de Almeida, Edmund Chada Baracat, Ayman Al-Hendy, Qiwei Yang, Katia Candido Carvalho
Leiomyomas (LMs) are the most frequent uterine benign tumors, representing the leading cause of hysterectomy indications worldwide. They are highly associated with women's reproductive complications, and endocrine disruptors may influence their etiology. In this sense, air pollution represents a relevant hormonal disruptor that acts on key signaling pathways, resulting in tumor development and infertility. Our goal was to evaluate submucosal LM samples from patients living in the metropolitan and Sao Paulo city regions, focusing on genes involved in tumor development and infertility features...
January 26, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/36156078/small-molecule-wnt-inhibitors-are-a-potential-novel-therapy-for-intestinal-fibrosis-in-crohns-disease
#24
JOURNAL ARTICLE
Amy Lewis, Saray Sánchez, Giulio Berti, Belen Pan-Castillo, Anke Nijhuis, Shameer Mehta, Liliane Eleid, Hannah Gordon, Radha Gadhok, Annamaria Minicozzi, Joanne Chin-Aleong, Roger Feakins, Robert Kypta, James Oliver Lindsay, Andrew Silver, Christopher Kimberley
Intestinal fibrosis and stricture formation is an aggressive complication of Crohns disease (CD), linked to increased morbidity and costs. This study investigates the contribution of Wnt signalling to intestinal fibrogenesis, considers potential crosstalk between Wnt and TGFβ signalling pathways and assesses the therapeutic potential of small-molecule Wnt inhibitors.β-catenin expression was explored by immunohistochemistry in FFPE tissue from patient-matched non-strictured (NSCD) and strictured (SCD) intestine (n=6 pairs)...
September 26, 2022: Clinical Science (1979-)
https://read.qxmd.com/read/36017689/sclerostin-is-involved-in-osteogenic-transdifferentiation-of-vascular-smooth-muscle-cells-in-chronic-kidney-disease-associated-vascular-calcification-with-non-canonical-wnt-signaling
#25
JOURNAL ARTICLE
Qiong Xiao, Yun Tang, Haojun Luo, Sipei Chen, Rong Chen, Zhe Yan, Lei Pu, Li Wang, Guisen Li, Yi Li
Vascular calcification is prominent in patients with chronic kidney disease (CKD) and is a strong predictor of cardiovascular mortality in the CKD population. However, the mechanism underlying CKD-associated vascular calcification remains unclear. To identify potential therapeutic targets, a 5/6 nephrectomy rat model was established by feeding of a high-phosphorous diet as the CKD group and compared with sham group rats at 4 and 16 weeks. Sequencing analyses of the rat aorta revealed 643 upregulated and 1023 downregulated genes at 4 weeks, as well as 899 upregulated and 1185 downregulated genes at 16 weeks in the CKD group compared to the sham group...
December 2022: Renal Failure
https://read.qxmd.com/read/35987514/phosphate-promotes-osteogenic-differentiation-through-non-canonical-wnt-signaling-pathway-in-human-mesenchymal-stem-cells
#26
JOURNAL ARTICLE
Shumin Rui, Takuo Kubota, Yasuhisa Ohata, Kenichi Yamamoto, Makoto Fujiwara, Shinji Takeyari, Keiichi Ozono
BACKGROUND: Phosphate is indispensable in osteogenesis and mineralization. However, mechanisms by which phosphate enhances osteogenic differentiation are not fully understood. In this study, we studied the effect of phosphate on osteogenic differentiation as well as signaling pathways induced by phosphate in the process. METHOD: Induced human bone marrow-derived mesenchymal stem cells differentiation into osteoblasts by the change of media containing β-glycerophosphate (GP), 1 mM inorganic phosphate, or 3 mM inorganic phosphate (Pi)...
August 18, 2022: Bone
https://read.qxmd.com/read/35770636/-silk-fibroin-collagen-composite-hydrogels-with-different-matrix-stiffness-influence-the-growth-and-phenotype-of-human-mammary-epithelial-cells
#27
JOURNAL ARTICLE
Yi-Ning Zhang, Hui Jiao, Li-Na Guo, Xin-Yu Ma, Gao-Cheng Gai, Jin-Tao Zhang, Wen-Qing Yang, Xiu-Li Wang
Extracellular matrix (ECM) stiffness is closely related to the physiological and pathological states of breast tissue. The current study was aimed to investigate the effect of silk fibroin/collagen composite hydrogels with adjustable matrix stiffness on the growth and phenotype of normal breast epithelial cells. In this study, the enzymatic reaction of horseradish peroxidase (HRP) with hydrogen peroxide (H2 O2 ) was used to change the degree of cross-linking of the silk fibroin solution. The rotational rheometer was used to characterize the composite hydrogel's biomechanical properties...
June 25, 2022: Sheng Li Xue Bao: [Acta Physiologica Sinica]
https://read.qxmd.com/read/35673567/the-role-of-jagged1-as-a-dynamic-switch-of-cancer-cell-plasticity-in-pdac-assembloids
#28
JOURNAL ARTICLE
Jae-Il Choi, John Hoon Rim, Sung Ill Jang, Joon Seong Park, Hak Park, Jae Hee Cho, Jong-Baeck Lim
Background: Pancreatic ductal adenocarcinoma (PDAC), which commonly relapses due to chemotherapy resistance, has a poor 5-year survival rate (< 10%). The ability of PDAC to dynamically switch between cancer-initiating cell (CIC) and non-CIC states, which is influenced by both internal and external events, has been suggested as a reason for the low drug efficacy. However, cancer cell plasticity using patient-derived PDAC organoids remains poorly understood. Methods: First, we successfully differentiated CICs, which were the main components of PDAC organoids, toward epithelial ductal carcinomas...
2022: Theranostics
https://read.qxmd.com/read/35534526/non-canonical-wnt-signaling-participates-in-jagged1-induced-osteo-odontogenic-differentiation-in-human-dental-pulp-stem-cells
#29
JOURNAL ARTICLE
Chatvadee Kornsuthisopon, Ajjima Chansaenroj, Jeeranan Manokawinchoke, Kevin A Tompkins, Nopadon Pirarat, Thanaphum Osathanon
Osteoblast differentiation requires the interaction of various cell signaling pathways to modulate cell responses. Notch and Wnt signaling are among the crucial pathways that control numerous biological processes, including osteo/odontogenic differentiation. The aim of the present study was to examine the involvement of Wnt signaling in the Jagged1-induced osteo/odontogenic differentiation in human dental pulp stem cells (hDPSCs). The Wnt-related gene expression was analyzed from publicly available data of Jagged1-treated human dental pulp cells...
May 9, 2022: Scientific Reports
https://read.qxmd.com/read/35527292/neuroprotective-effect-of-baicalein-against-oxaliplatin-induced-peripheral-neuropathy-impact-on-oxidative-stress-neuro-inflammation-and-wnt-%C3%AE-catenin-signaling
#30
JOURNAL ARTICLE
Simrandeep Jugait, Aparna Areti, Karthika Nellaiappan, Priyanka Narwani, Priya Saha, Ravichandiran Velayutham, Ashutosh Kumar
Oxaliplatin, an effective anti-cancer agent used in the treatment of colorectal cancer, is associated with severe dose-limiting side effects like peripheral neuropathy, which currently remains a major unmet clinical need. This study was designed to investigate the possible neuroprotective potential of a bioflavonoid, baicalein in an experimental model of oxaliplatin-induced peripheral neuropathy. Rats were administered with a dose of 4 mg/kg oxaliplatin i.p. twice per week for four weeks, and were evaluated for behavioral and functional nerve parameters, followed by biochemical, immunohistochemical and western blot analysis...
July 2022: Molecular Neurobiology
https://read.qxmd.com/read/35447159/wnt5b-plays-a-negative-role-in-antibacterial-response-in-pacific-white-shrimp-penaeus-vannamei
#31
JOURNAL ARTICLE
Linwei Yang, Zi-Ang Wang, Hongliang Zuo, Zhixun Guo, Shaoping Weng, Jianguo He, Xiaopeng Xu
The Wnt family genes are essentially implicated in development and growth in animals. Accumulating clues have pointed to the importance of Wnts in invertebrate immunity, but the underlying mechanisms are still unclear to date. The Wnt5b has been known to promote white spot syndrome virus (WSSV) infection in shrimp but its role in antibacterial response remains unclear. In the current study, we focused on the involvement of Wnt5b in Vibrio parahaemolyticus infection in Pacific white shrimp Penaeus vannamei. We demonstrated that the expression of Wnt5b was regulated by the IMD-Relish and JAK-STAT pathways but not the Dorsal pathway and was suppressed upon bacterial infection...
April 18, 2022: Developmental and Comparative Immunology
https://read.qxmd.com/read/35142609/the-e3-ubiquitin-ligase-mindbomb1-controls-planar-cell-polarity-dependent-convergent-extension-movements-during-zebrafish-gastrulation
#32
JOURNAL ARTICLE
Vishnu Muraleedharan Saraswathy, Akshai Janardhana Kurup, Priyanka Sharma, Sophie Polès, Morgane Poulain, Maximilian Fürthauer
Vertebrate Delta/Notch signaling involves multiple ligands, receptors and transcription factors. Delta endocytosis - a critical event for Notch activation - is however essentially controlled by the E3 Ubiquitin ligase Mindbomb1 (Mib1). Mib1 inactivation is therefore often used to inhibit Notch signaling. However, recent findings indicate that Mib1 function extends beyond the Notch pathway. We report a novel Notch-independent role of Mib1 in zebrafish gastrulation. mib1 null mutants and morphants display impaired Convergence Extension (CE) movements...
February 10, 2022: ELife
https://read.qxmd.com/read/35008901/brachygnathia-inferior-in-cloned-dogs-is-possibly-correlated-with-variants-of-wnt-signaling-pathway-initiators
#33
JOURNAL ARTICLE
Yong-Ho Choe, Tai-Young Hur, Sung-Lim Lee, Seunghoon Lee, Dajeong Lim, Bong-Hwan Choi, Haeyun Jeong, Jin-Gu No, Sun A Ock
Abnormalities in animals cloned via somatic cell nuclear transfer (SCNT) have been reported. In this study, to produce bomb-sniffing dogs, we successfully cloned four healthy dogs through SCNT using the same donor genome from the skin of a male German shepherd old dog. Veterinary diagnosis (X-ray/3D-CT imaging) revealed that two cloned dogs showed normal phenotypes, whereas the others showed abnormal shortening of the mandible (brachygnathia inferior) at 1 month after birth, even though they were cloned under the same conditions except for the oocyte source...
January 1, 2022: International Journal of Molecular Sciences
https://read.qxmd.com/read/34906330/estrogen-receptor-alpha-and-nfatc1-bind-to-a-bone-mineral-density-associated-snp-to-repress-wnt5b-in-osteoblasts
#34
JOURNAL ARTICLE
Sarocha Suthon, Jianjian Lin, Rachel S Perkins, John R Crockarell, Gustavo A Miranda-Carboni, Susan A Krum
Genetic factors and estrogen deficiency contribute to the development of osteoporosis. The single-nucleotide polymorphism (SNP) rs2887571 is predicted from genome-wide association studies (GWASs) to associate with osteoporosis but has had an unknown mechanism. Analysis of osteoblasts from 110 different individuals who underwent joint replacement revealed that the genotype of rs2887571 correlates with WNT5B expression. Analysis of our ChIP-sequencing data revealed that SNP rs2887571 overlaps with an estrogen receptor alpha (ERα) binding site...
December 2, 2021: American Journal of Human Genetics
https://read.qxmd.com/read/34791636/inhibition-of-the-bromodomain-and-extra-terminal-family-of-epigenetic-regulators-as-a-promising-therapeutic-approach-for-gastric-cancer
#35
JOURNAL ARTICLE
Sun Kyoung Kang, Hyun Joo Bae, Woo Sun Kwon, Tae Soo Kim, Kyoo Hyun Kim, Sejung Park, Seo Young Yu, Jihyun Hwang, Juin Park, Hyun Cheol Chung, Sun Young Rha
PURPOSE: Epigenetic dysregulation is a common characteristic of cancers, including gastric cancer (GC), and contributes to cancer development and progression. Although the efficacy of BET (an epigenetic regulator) inhibition has been demonstrated in various cancer types, predictive genetic markers of its efficacy in GC are currently lacking. Therefore, we aimed to identify markers that predict the response of BET inhibition in GC and, suggest an effective treatment regimen through combined therapy...
December 2021: Cellular Oncology (Dordrecht)
https://read.qxmd.com/read/34635443/wnt5b-in-cellular-signaling-pathways
#36
REVIEW
Rachel S Perkins, Sarocha Suthon, Gustavo A Miranda-Carboni, Susan A Krum
The Wnt signaling ligand WNT5B is implicated in various developmental pathways, both in normal and pathological physiology. Most of the research on WNT5B has been associated with expression analysis and disease states, leaving the signaling pathways underexplored. Here, we review the current understandings of WNT5B's regulation of signal transduction, from receptors to downstream mediators and transcription factors. We also describe its roles in β-catenin-dependent and β-catenin-independent (Planar Cell Polarity and Wnt/Ca2+ ) Wnt signaling...
May 2022: Seminars in Cell & Developmental Biology
https://read.qxmd.com/read/34591076/glypican-4-mediates-wnt-transport-between-germ-layers-via-signaling-filopodia
#37
JOURNAL ARTICLE
Bo Hu, Juan J Rodriguez, Anurag Kakkerla Balaraju, Yuanyuan Gao, Nhan T Nguyen, Heston Steen, Saeb Suhaib, Songhai Chen, Fang Lin
Glypicans influence signaling pathways by regulating morphogen trafficking and reception. However, the underlying mechanisms in vertebrates are poorly understood. In zebrafish, Glypican 4 (Gpc4) is required for convergence and extension (C&E) of both the mesoderm and endoderm. Here, we show that transgenic expression of GFP-Gpc4 in the endoderm of gpc4 mutants rescued C&E defects in all germ layers. The rescue of mesoderm was likely mediated by Wnt5b and Wnt11f2 and depended on signaling filopodia rather than on cleavage of the Gpc4 GPI anchor...
December 6, 2021: Journal of Cell Biology
https://read.qxmd.com/read/34572445/master-regulators-of-epithelial-mesenchymal-transition-and-wnt-signaling-pathways-in-juvenile-nasopharyngeal-angiofibromas
#38
JOURNAL ARTICLE
Naiade Calanca, Sara Martoreli Silveira Binato, Sabrina Daniela da Silva, Helena Paula Brentani, Luiz Ubirajara Sennes, Clóvis Antonio Lopes Pinto, Maria Aparecida Custódio Domingues, Carlos Eduardo Fonseca-Alves, Claudia Aparecida Rainho, Silvia Regina Rogatto
Juvenile nasopharyngeal angiofibroma (JNA) is a rare fibrovascular benign tumor showing an invasive growth pattern and affecting mainly male adolescents. We investigated the role of epithelial-mesenchymal transition (EMT) and WNT signaling pathways in JNA. Gene expression profiles using nine JNA paired with four inferior nasal turbinate samples were interrogated using a customized 2.3K microarray platform containing genes mainly involved in EMT and WNT/PI3K pathways. The expression of selected genes ( BCL2 , CAV1 , CD74 , COL4A2 , FZD7 , ING1 , LAMB1 , and RAC2 ) and proteins (BCL2, CAV1, CD74, FZD7, RAF1, WNT5A, and WNT5B) was investigated by RT-qPCR (28 cases) and immunohistochemistry (40 cases), respectively...
September 18, 2021: Biomedicines
https://read.qxmd.com/read/34536574/exosome-mediated-circ_0001846-participates-in-il-1%C3%AE-induced-chondrocyte-cell-damage-by-mir-149-5p-dependent-regulation-of-wnt5b
#39
JOURNAL ARTICLE
Chunhui Zhu, Kai Shen, Weibo Zhou, Hao Wu, Yaojun Lu
AIMS: Osteoarthritis (OA) is the leading cause of physical disability in middle-aged and elderly people globally. Previous studies have revealed that circular RNA (circRNA) is involved in the pathogenesis of OA. In this study, we studied the role of circ_0001846 in interleukin-1β (IL-1β)-induced OA progression. METHODS: Twenty-one patients with OA and 17 volunteers were recruited for the collection of articular cartilage tissues. The expression of circ_0001846, microRNA-149-5p (miR-149-5p) and Wingless-type MMTV integration site family, member 5B (WNT5B) was detected by quantitative real-time polymerase chain reaction...
September 15, 2021: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://read.qxmd.com/read/34368954/wnt5b-promotes-vascular-smooth-muscle-cell-dedifferentiation-via-mitochondrial-dynamics-regulation-in-chronic-thromboembolic-pulmonary-hypertension
#40
JOURNAL ARTICLE
Feng Wang, Yanan Zhen, Chaozeng Si, Cheng Wang, Lin Pan, Yang Chen, Xiaopeng Liu, Jie Kong, Qiangqiang Nie, Mingsheng Sun, Yongxin Han, Zhidong Ye, Peng Liu, Jianyan Wen
Chronic thromboembolic pulmonary hypertension (CTEPH) is characterized by proliferative vascular remodeling. Abnormal vascular smooth muscle cell (VSMC) phenotype switching is crucial to this process, highlighting the need for VSMC metabolic changes to cover cellular energy demand in CTEPH. We report that elevated Wnt family member 5B (WNT5B) expression is associated with vascular remodeling and promotes VSMC phenotype switching via mitochondrial dynamics regulation in CTEPH. Using primary culture of pulmonary artery smooth muscle cells, we show that high WNT5B expression activates VSMC proliferation and migration and results in mitochondrial fission via noncanonical Wnt signaling in CTEPH...
January 2022: Journal of Cellular Physiology
keyword
keyword
167020
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.