Florian Perrin, Lauren C Anderson, Shane P C Mitchell, Priyanka Sinha, Yuliia Turchyna, Masato Maesako, Mei C Q Houser, Can Zhang, Steven L Wagner, Rudolph E Tanzi, Oksana Berezovska
The recently discovered interaction between presenilin 1 (PS1), a catalytic subunit of γ-secretase responsible for the generation of amyloid-β(Aβ) peptides, and GLT-1, the major glutamate transporter in the brain (EAAT2 in the human) may provide a mechanistic link between two important pathological aspects of Alzheimer's disease (AD): abnormal Aβoccurrence and neuronal network hyperactivity. In the current study, we employed a FRET-based approach, fluorescence lifetime imaging microscopy (FLIM), to characterize the PS1/GLT-1 interaction in its native environment in the brain tissue of sporadic AD (sAD) patients...
November 7, 2023: Research Square