Charlie J Childs, Emily M Holloway, Caden W Sweet, Yu-Hwai Tsai, Angeline Wu, Abigail Vallie, Madeline K Eiken, Meghan M Capeling, Rachel K Zwick, Brisa Palikuqi, Coralie Trentesaux, Joshua H Wu, Oscar Pellon-Cardenas, Charles J Zhang, Ian A Glass, Claudia Loebel, Qianhui Yu, J Gray Camp, Jonathan Z Sexton, Ophir D Klein, Michael P Verzi, Jason R Spence
Epithelial organoids derived from intestinal tissue, called 'enteroids', recapitulate many aspects of the organ in vitro, and can be used for biological discovery, personalized medicine, and drug development. Here, we interrogated the cell signaling environment within the developing human intestine to identify niche cues that may be important for epithelial development and homeostasis. We identify an EGF family member, EPIREGULIN (EREG), which is robustly expressed in the developing human crypt. Enteroids generated from the developing human intestine grown in standard culture conditions, which contain EGF, are dominated by stem and progenitor cells, feature little differentiation and no spatial organization...
February 23, 2023: JCI Insight