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Proteomic , serum marker, cancer detection, cancer metastasis

Eleni Zografos, Athanasios K Anagnostopoulos, Aggeliki Papadopoulou, Evangelia Legaki, Flora Zagouri, Evangelos Marinos, George T Tsangaris, Maria Gazouli
BACKGROUND: To date, the elucidation of serum protein alterations in male breast cancer (MBC) has not been extensively studied, due to the rarity of the disease. MATERIALS AND METHODS: In the present work, two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) were employed to detect differences in serum protein expression between patients with MBC and healthy controls. RESULTS: A panel of differentially expressed serum proteins was identified, including proteins involved in the regulation of the cell cycle [e...
March 2019: Cancer Genomics & Proteomics
Hailong Fu, Huan Yang, Xu Zhang, Bo Wang, Jiahui Mao, Xia Li, Mei Wang, Bin Zhang, Zixuan Sun, Hui Qian, Wenrong Xu
BACKGROUND: Exosomes are critically involved in cancer development and progression. The exosomal contents have been suggested as ideal cancer biomarkers. In this study, we investigated the expression of exosomal proteins in the serum of gastric cancer patients and their roles in gastric cancer. METHODS: The proteomic profile of exosomes from the serum of gastric cancer patients was detected by using LC-MS/MS. The expression of TRIM3 in exosomes from the serum of gastric cancer patients and healthy controls was assessed by ELISA and western blot...
July 21, 2018: Journal of Experimental & Clinical Cancer Research: CR
Zarina S Lalmahomed, Mirelle Ee Bröker, Nick A van Huizen, Robert R J Coebergh van den Braak, Lennard Jm Dekker, Dimitris Rizopoulos, Cornelis Verhoef, Ewout W Steyerberg, Theo M Luider, Jan Nm IJzermans
The clinical efficacy of carcinoembryonic antigen (CEA) as a marker of colorectal liver metastasis is limited, motivating a search for new biomarkers. Recently, urine proteomic analysis revealed AGPP(-OH)GEAGKP(-OH)GEQGVP(-OH)GDLGAP(-OH)GP (AGP), a promising peptide for this application. This study aimed to determine whether combining urine AGP testing with serum CEA analyses improves the sensitivity of detecting colorectal liver metastases. Urine samples from 100 patients with CRLM were collected prospectively and compared to three control groups: healthy kidney donors, patients who were relapse-free for 24 months after curative CRLM surgery, and primary colorectal cancer patients...
2016: American Journal of Cancer Research
Hui Zhang, Hao Li, Fei Guo, Da Zhang, Heying Yang, Jiaxiang Wang
OBJECTIVE: To screen and identify the serum specific protein markers of patients with gastric cancer by proteomics technology, and to provide more comprehensive serum protein fingerprint model for the early diagnosis of gastric cancer. METHODS: Preoperative and postoperative blood samples were collected from 60 gastric cancer patients. Mass spectrometry (SELDI-TOF-MS) technology was used to detect and screen serum specific proteins in gastric cancer patients(preoperative group, postoperative group, metastasis group), and the result was compared with normal control group...
March 2016: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
Bashar Zeidan, Thomas R Jackson, Samantha E T Larkin, Ramsey I Cutress, Gary R Coulton, Margaret Ashton-Key, Nick Murray, Graham Packham, Vassilis Gorgoulis, Spiros D Garbis, Paul A Townsend
Breast cancers are the most common cancer-affecting women; critically the identification of novel biomarkers for improving early detection, stratification and differentiation from benign tumours is important for the reduction of morbidity and mortality.To identify and functionally characterise potential biomarkers, we used mass spectrometry (MS) to analyse serum samples representing control, benign breast disease (BBD) and invasive breast cancer (IDC) patients. Complementary and multidimensional proteomic approaches were used to identify and validate novel serum markers...
August 28, 2015: Oncotarget
Betul Celik, Arzu Didem Yalcin, Gizem Esra Genc, Saadet Gumuslu
PURPOSE: The purpose of this study was to evaluate the soluble Apo-2L (sApo-2L) levels in the ascitic fluid and to study its potential in detecting malignant ascites and soluble CD200 (sCD200,sOX-2) levels so as to predict its clinical usage for detecting stage 4 metastatic endometrial, ovarian and breast cancer in serum samples. METHODS: Ascitic fluid from 53 and blood from 25 subjects without known malignancy on admission were collected. There were 14 breast cancer (BC), 17 ovarian cancer (OC) and 19 endometrial cancer (EC) patients diagnosed later on...
January 2015: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
Ming Guan, Xiaofan Chen, Yingyu Ma, Lihua Tang, Lei Guan, Xuefeng Ren, Bo Yu, Wei Zhang, Bing Su
Metastatic melanoma, the primary cause of skin cancer-related death, warrants new diagnostic and therapeutic approaches that target the regulatory machinery at molecular level. The heterogeneity and complexity of melanoma result in the difficulty to find biomarkers and targets for early detection and treatment. Here, we investigated metastasis-associated proteins by comparing the proteomic profiles of primary cutaneous melanomas to their matched lymph node metastases, which minimizes heterogeneity among samples from different patients...
April 2015: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
Sujatha Bhat, Vasudevan Bhaskaran Kartha, Lavanya Rai, Santhosh Chidangil
Cervical cancer, the second most common cancer in women, progresses silently over long periods before producing any clinical manifestation. Research in early detection of this condition using proteomic techniques is of very recent origin. We used high-performance liquid chromatography combined with laser-induced fluorescence method in our lab to record the protein profiles of tissue homogenate, cell lysate and serum samples of normal and different stages of malignant conditions of the cervix. Information on protein markers in the protein profiles was derived using various data processing methods including curve resolution...
January 2015: Journal of Chromatographic Science
J Shi, J Liu, L Liao, Y Guo, H Wang, W Hu, T Hu
AIM: Detection of novel tumor biomarker will aid in diagnosis of early-stage small cell lung cancer (SCLC). The purpose of this study was to identify novel tumor biomarker in serum from patients with SCLC using a proteomics-based approach. METHODS: Sera were analyzed before the initiation of chemotherapy. Serum proteins of SCLC patients and healthy controls were collected and separated by 2-D fluorescence differential gel electrophoresis (2-D DIGE). Positive spots were analyzed by LC-MS/MS...
April 2014: Minerva Medica
Sho Minami, Ryo Nagashio, Junpei Ueda, Kazumasa Matsumoto, Naoki Goshima, Manabu Hattori, Kazuo Hachimura, Masatsugu Iwamura, Yuichi Sato
Secreted proteins play essential roles in the process of tumorigenesis, and the analysis of tumor-secreted proteins has been suggested as a promising strategy for identifying cancer biomarkers. In this study, we performed proteomic analysis to identify proteins secreted from bladder cancer cell lines that are recognized by autoantibodies in sera from patients with bladder cancer. In addition,autoantibodies against the identified proteins were validated using a dot-blot array with sera from patients with bladder cancer and normal controls...
2014: Biomedical Research
Xia Zou, Bo Feng, Taotao Dong, Guoquan Yan, Binbin Tan, Hao Shen, Ao Huang, Xiu Zhang, Menghui Zhang, Pengyuan Yang, Minhua Zheng, Yan Zhang
UNLABELLED: Colorectal cancer (CRC) is one of the most prevalent cancers worldwide. The discovery of non-invasive biomarker candidates for diagnosis and prognosis is important for the management of CRC. In this study, we performed proteomic profiling of serum from patients with different stages of CRC using a 2D-LC-MS/MS based approach combined with the APEX quantitative method. 917 proteins were identified and 93 were differentially expressed in normal and three patient groups (stages I, II and III)...
December 6, 2013: Journal of Proteomics
Qing-Lian Wan, Xiang-Sheng Hou, Guang Zhao
Esophageal cancer (EC) is one of the most common malignant tumors, and the incidence of esophageal squamous cell carcinoma (ESCC) is highest in China. Early diagnosis and effective monitoring are keys to comprehensive treatment and discovering tumor metastases and recurrence in time. The aim of this study was to confirm serum peptidome pattern utility for diagnosis of ESCC, and assessment of operation success, postoperative chemotherapy results, tumor metastasis and recurrence. Serum samples were collected from 61 patients treated with surgery and chemotherapy and 20 healthy individuals...
2013: Asian Pacific Journal of Cancer Prevention: APJCP
Changchuan Pan, Yalan Tao, Ming Zhao, Wang Li, Zilin Huang, Jing Gao, Yanhen Wu, Jingrui Yu, Peihong Wu, Yunfei Xia, Jin Lu
Metastasis is the main cause of cancer-related mortality; patients with liver metastases (LM) have the worst prognosis among patients with nasopharyngeal carcinoma (NPC). However, at present, few biomarkers for detecting organ-specific metastasis have been identified. Proteomics, an ultra-sensitive analytical technique, can detect molecular changes before organ-specific metastasis occurs. Analysis with matrix-assisted, laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS), combined with magnetic chemical affinity beads is a new technique for evaluating protein separation...
June 2012: Experimental and Therapeutic Medicine
Jia Fan, Xiaoyong Deng, James W Gallagher, Haiyu Huang, Yi Huang, Jianguo Wen, Mauro Ferrari, Haifa Shen, Ye Hu
Breast cancer accounted for 15 per cent of total cancer deaths in female patients in 2010. Although significant progress has been made in treating early-stage breast cancer patients, there is still no effective therapy targeting late-stage metastatic breast cancers except for the conventional chemotherapy interventions. Until effective therapy for later-stage cancers emerges, the identification of biomarkers for the early detection of tumour metastasis continues to hold the key to successful management of breast cancer therapy...
May 28, 2012: Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
Peter Findeisen, Michael Neumaier
Clinical proteomic profiling by mass spectrometry (MS) aims at uncovering specific alterations within mass profiles of clinical specimens that are of diagnostic value for the detection and classification of various diseases including cancer. However, despite substantial progress in the field, the clinical proteomic profiling approaches have not matured into routine diagnostic applications so far. Their limitations are mainly related to high-abundance proteins and their complex processing by a multitude of endogenous proteases thus making rigorous standardization difficult...
January 2012: Proteomics. Clinical Applications
Liang Wang, Dan Su, Huai-Jun Yan, Jian-Hua Xu, Zhi-Guo Zheng, Yan-Jun Hu, Xiao-Dan Pan, Xiao-Wen Ding, Chun Chen, Bin Chen, Wei-Min Mao, Xu-Li Meng
The aim of this study was to detect the pretreatment serum protein profiles of breast cancer patients by mass spectrometry (MS) to screen candidate tumor biomarkers, which will supply a simple, accurate, and minimally invasive method to predict the axillary lymph node metastasis of breast cancer. We used magnetic bead-based weak cation-exchange chromatography followed by matrix-assisted laser desorption and ionization time-of-flight MS to detect proteins in the sera of 54 cases of axillary node-negative breast cancer, 47 cases of axillary node-positive breast cancer, and 101 healthy controls...
November 2011: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
Chien-Wei Tseng, Jyh-Chin Yang, Chiung-Nien Chen, Hsuan-Cheng Huang, Kai-Neng Chuang, Chen-Ching Lin, Hong-Shiee Lai, Po-Huang Lee, King-Jen Chang, Hsueh-Fen Juan
Gastric cancer is the second most common cause of cancer deaths worldwide and due to its poor prognosis, it is important that specific biomarkers are identified to enable its early detection. Through 2-D gel electrophoresis and MALDI-TOF-TOF-based proteomics approaches, we found that 14-3-3β, which was one of the proteins that were differentially expressed by 5-fluorouracil-treated gastric cancer SC-M1 cells, was upregulated in gastric cancer cells. 14-3-3β levels in tissues and serum were further validated in gastric cancer patients and controls...
June 2011: Proteomics
Chibo Liu, Chunqin Pan, Jianmin Shen, Haibao Wang, Liang Yong
The aim of present study is to study the serum protein fingerprint of patients with colorectal cancer (CRC) and to screen protein molecules that are closely related to colorectal cancer during the onset and progression of the disease with Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Serum samples from 144 patients with CRC and 120 healthy volunteers were adopted in present study. Weak cation exchange (WCX) magnetic beads and PBSII-C protein chips reader (Ciphergen Biosystems Ins...
2011: International Journal of Medical Sciences
L Zhao, Y Liu, X Sun, K Peng, Y Ding
Colorectal cancer (CRC) is the second leading cause of cancer death in the human population worldwide. In China, CRC ranks fifth among cancer deaths, with a continuous increase in the incidence. Most CRC patients have lymph node metastasis (LNM) when first diagnosed. Two-dimensional (2D) serum proteome analysis may be useful in discovering new biomarkers that may aid in the diagnosis and therapy of CRC patients. To determine the tumour metastasis-specific antigen markers of CRC metastasis, sera from 32 patients with non-LNM CRC and 40 patients with LNM CRC were selected for screening using 2D serum proteome analysis combined with mass spectrometry (MS)...
February 2011: Journal of Comparative Pathology
Yifeng He, Xin Wu, Xiaohui Liu, Guoquan Yan, Congjian Xu
Peritoneal implantation is the most common metastatic pattern of epithelial ovarian cancer, and the five-year survival rate of patients is dramatically decreased when large-scale peritoneal metastasis occurs. This study aimed to determine serum proteins that could be used to detect early peritoneal metastasis of ovarian cancer. The secreted (or shed) proteins of the ovarian cancer cell line SKOV-3 were analyzed using LC-MS/MS, and 97 proteins were identified in the SKOV-3 culture supernatant. After the SKOV-3 cells were xenografted into the peritoneal cavities of nude mice, 3 of the 97 proteins were detected in animal sera...
December 3, 2010: Journal of Proteome Research
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