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D Bharathi, R Hari Krishna, B Siddlingeshwar, Darshan Devang Divakar, Abdulaziz Abdullah Alkheraif
In spite copper oxide being one of the essential micronutrient, copper oxide in its nano size is found to be toxic in nature; this instigates for the detection of copper oxides in trace levels. In the present study, we demonstrate simple cost effective detection method for CuO/Cu2 O using carbon quantum dots (CQD) by fluorescence quenching technique. CuO/Cu2 O nanoparticles are synthesised by mere variation of fuel ratio by solution combustion technique. The resulting oxides are characterized by various analytical and spectroscopic techniques...
February 4, 2019: Journal of Hazardous Materials
Zuzana Gajdosechova, Zoltan Mester
The need to assess the human and environmental risks of nanoparticles (NPs) has prompted an adaptation of existing techniques and the development of new ones. Nanoparticle analysis poses a great challenge as the analytical information has to consider both physical (e.g. size and shape) and chemical (e.g. elemental composition) state of the analyte. Furthermore, one has to contemplate the transformation of NPs during the sample preparation and provide sufficient information about the new species derived from such alteration...
February 14, 2019: Analytical and Bioanalytical Chemistry
Rafiye Çiftçiler, Salih Aksu, Neslihan Dikmenoğlu Falkmarken, İbrahim Celalettin Haznedaroğlu
Background/aim: Ankaferd hemostat (ABS; Ankaferd blood stopper, İstanbul, Turkey) is a prohemostatic agent affecting erythrocytes. The hemostatic action of ABS depends upon fibrinogen gamma chain, prothrombin, and red blood cells. The aim of this study was to assess the effects of ABS on erythrocyte aggregation via hemorheological analyses. Materials and methods: To measure erythrocyte aggregation, blood samples were obtained from healthy, nonsmoker volunteers who had not taken any medication in the previous 10 days...
February 11, 2019: Turkish Journal of Medical Sciences
Miriam Luhnen, Barbara Prediger, Edmund A M Neugebauer, Tim Mathes
INTRODUCTION: The number of systematic reviews of health economic evaluations (SR-HEs) is increasing. We aimed at providing a detailed overview of the characteristics and applied methods in recently published SR-HEs. METHODS: We searched MEDLINE (03/2017) for SR-HEs published since 2015 using validated search filters. We included studies that performed a systematic review of full economic evaluations and searched at least one electronic database. We extracted data in a standardized, beforehand piloted form that was deduced from the items of the PRISMA and CHEERS checklists...
February 13, 2019: Research Synthesis Methods
M Okamoto, Y Takahashi, S Komichi, M Ali, M Watanabe, M Hayashi
AIM: To evaluate the dentinogenetic effects of tissue inhibitor of metalloprotease (TIMP1) on human pulp cells in vitro and rat pulp tissue in vivo. METHODOLOGY: The effect of TIMP1 on pulp cell functions related to hard tissue formation as part of the wound healing process (i.e. biocompatibility, proliferation, differentiation and mineralized nodule formation) was evaluated in vitro and using a direct pulp capping experimental animal model in vivo. The effects of different-sized cavity preparations on hard tissue formation induced by ProRoot MTA at 2 weeks were evaluated using micro-computed tomography (micro-CT)...
February 13, 2019: International Endodontic Journal
Nicolas Riesen, Mark Lockrey, Kate Badek, Hans Riesen
There is great interest in the use of highly-efficient all-inorganic halide perovskites CsnPbBr2+n for optoelectronic applications. There however remains considerable debate as to the origins of the green luminescence in the zero-dimensional phase of the perovskite Cs4PbBr6, with theories suggesting it originates either from defects in the Cs4PbBr6 lattice or CsPbBr3 impurities/inclusions. The confusion has arisen due to the two phases being miscible and typically co-existing. Moreover, low impurity levels of CsPbBr3 in Cs4PbBr6 are difficult to detect by XRD measurements, yet have much stronger photoluminescence than bulk CsPbBr3 that exhibits quenching, further contributing to the confusion as to the origins of the green photoluminescence...
February 14, 2019: Nanoscale
Hiroshi Takahashi, Ryota Watanabe, Kenichi Nishimura, Taro Moriwaki
A recent study has shown that the addition of ceramide to adhesive materials of medical sheets or tapes improves or maintains skin barrier functions despite the irritation and damage caused by the repeated removal of the sheet or tape. This fact may imply that ceramide molecules are released from the adhesive material and penetrate the skin. In this study, we investigated whether ceramide molecules are released from a UV-curable acrylic adhesive resin (acResin® ) sheet containing ceramide molecules attached to a cultured skin sample by estimating the local ceramide concentration from the data obtained by synchrotron Fourier transform infrared micro-spectroscopy analysis...
February 13, 2019: Journal of Oleo Science
Helena Čelešnik, Uroš Potočnik
We report two restriction enzyme-based approaches for generating clean locus-specific unmethylated controls for methylation-sensitive high-resolution melting (MS-HRM) analyses. These unmethylated standards are derived from DNA treated with the demethylating agent 5-aza-2-deoxycytidine (5-Aza-dc). By using them, we overcome a limitation of 5-Aza-dc treatment - incomplete demethylation at various genomic regions. When 5-Aza-dc-treated DNA is used directly as unmethylated MS-HRM standard, partially demethylated DNA can give false methylation results...
February 14, 2019: BioTechniques
Emily W Sun, Alyce M Martin, David A Wattchow, Dayan deFontgalland, Philippa Rabbitt, Paul Hollington, Richard L Young, Damien J Keating
Context: The anti-diabetic drug Metformin causes weight loss, but the underlying mechanisms are unclear. Recent clinical studies show that metformin increases plasma levels of the anorectic gut hormone, PYY, but whether this is through a direct effect on the gut is unknown. Objective: We hypothesised that exposure of human gut mucosal tissue to metformin would acutely trigger PYY secretion. Design, Setting, Participants, and Interventions: Mucosal tissue was prepared from 46 human colonic and 9 ileal samples obtained after surgical resection and ex vivo secretion assays performed...
February 12, 2019: Journal of Clinical Endocrinology and Metabolism
Gizem Ert Uuml Rk Bergdahl, Tilde Andersson, Maria Allhorn, Sofie Yngman, Rainer Timm, Rolf Lood
In this study, a surface plasmon resonance biosensor was developed for the detection and quantification of a secreted bacterial factor (RoxP) from skin. Molecular imprinting method was used for the preparation of sensor chips and five different monomer-cross linker compositions were evaluated for sensitivity, selectivity, affinity and kinetic measurements. The most promising MIP was characterized by using scanning electron microscopy, atomic force microscopy, and cyclic voltammetry. Limit of detection (LOD) value was calculated as 0...
February 13, 2019: ACS Sensors
Kantima Thongjued, Wilaiwan Chotigeat, Sara Bumrungsri, Phuvadol Thanakiatkrai, Thitika Kitpipit
Insect DNA barcoding is a species identification technique used in biodiversity assessment and ecological studies. However, DNA extraction can result in the loss of up to 70% of DNA. Recent research has reported that direct PCR can overcome this issue. However, the success rates could still be improved, and tissues used for direct PCR could not be reused for further genetic studies. Here, we developed a direct PCR workflow that incorporates a 2-min sample preparation in PBS-buffer step for fast and effective universal insect species identification...
February 13, 2019: Molecular Ecology Resources
Pengcheng Qian, Yingnan Qin, Yanlong Lyu, Yunfei Li, Lei Wang, Shuo Wang, Yaqing Liu
Highly sensitive detection of H2 O2 and glucose is critical for fundamental research and disease diagnosis. A ratiometric sensor can simultaneously afford two readout signals that provide an internally normalized response to change, thereby reducing false results and improving detection accuracy. A novel ratiometric fluorescent nanosensor for ultrasensitive detection of hydrogen peroxide and glucose was constructed on the basis of the peroxidase-like properties of a hierarchical cobalt/carbon nanotube hybrid nanocomplex (Co-CNT)...
February 13, 2019: Analytical and Bioanalytical Chemistry
Allan Henrique Depieri Cataneo, Diogo Kuczera, Ana Luiza Pamplona Mosimann, Emanuele Guimarães Silva, Álvaro Gil Araújo Ferreira, João Trindade Marques, Pryscilla Fanini Wowk, Claudia Nunes Duarte Dos Santos, Juliano Bordignon
BACKGROUND: The Zika virus (ZIKV) epidemics that affected South America in 2016 raised several research questions and prompted an increase in studies in the field. The transient and low viraemia observed in the course of ZIKV infection is a challenge for viral isolation from patient serum, which leads to many laboratories around the world sharing viral strains for their studies. C6/36 cells derived from Aedes albopictus larvae are commonly used for arbovirus isolation from clinical samples and for the preparation of viral stocks...
February 7, 2019: Memórias do Instituto Oswaldo Cruz
Asghar Amanpour, Oscar Zannou, Hasim Kelebek, Serkan Selli
Infusion-induced changes in the aroma, key odorants, and their odor activity values of Iranian endemic herbal (Gol-Gavzaban) tea obtained from shade-dried violet-blue petals of borage (Echium amoenum) were studied for the first time. Two hot teas and one cold tea were investigated and coded as 4MN (4 minutes/98°C), 16MN (16 minutes/98°C) and 24HR (24 hours/ambient temperature), respectively. Aromatic extracts of the tea samples were isolated by liquid-liquid extraction method and analyzed by gas chromatography-mass spectrometry-olfactometry (GC-MS-O) for the first time...
February 13, 2019: Journal of Agricultural and Food Chemistry
Linping Wang, Yue Meng, Chunxiu Zhang, Hongbo Xiao, Yunlong Li, Yueming Tan, Qingji Xie
The photoelectrochemistry (PEC) performance of TiO2 is somewhat limited by its wide band gap and low quantum efficiency, and the innovation of its composite materials provides a promising solution for an improved performance. Herein, a composite of an Au nanorod@TiO2 core-shell nanostructure (AuNR@TiO2) and a melanin-like L-DOPA polymer (PD) is designed and prepared, where the outer-layer PD tethered by the TiO2-hydroxyl complexation and the AuNR core can intensify the long-wavelength light harvesting, and the AuNR@TiO2 core-shell structure can strengthen the hot-electron transfer to TiO2...
February 13, 2019: ACS Applied Materials & Interfaces
Xiaojie Ni, Lei Lei, Yu Yu, Junjie Xie, Ming Li, Songwang Yang, Mei Wang, Jiandang Liu, Hongjun Zhang, Bangjiao Ye
Pristine and Br-doped H2 N = CHNH2 Pb(I1-x Br x )3 (FAPb(I1-x Br x )3 , Br content x = 0, 0.05, 0.15, 0.2, 0.3, and 0.4) films were prepared. The effect of Br-doping on phase stability, defect density, and performance of FAPb(I1-x Br x )3 was investigated by x-ray diffraction (XRD), scanning electron microscopy, ultraviolet-visible-near infrared absorbance spectroscopy, x-ray photoemission spectroscopy (XPS), Kelvin probe force microscopy (KPFM), positron annihilation spectroscopy, and current density-voltage (J-V) characteristics...
February 13, 2019: Nanotechnology
Masato Ueshima, Hirofumi Sakanakura
In the preparation of thin sections for microscopy, embedding and polishing processes in particular can change the composition and morphologies of samples. Soils and ashes are very fragile and solvent-susceptible, and appropriate sample preparation procedures have not been well-established. To improve the existing preparation methods and make them easier and faster, we embedded freeze-dried blocks, polished, and then examined these thin-section samples using polarization microscopy, laser microscopy, and field emission scanning electron microscopy with energy-dispersive X-ray spectrometry, and electron backscattered diffraction (EBSD)...
February 13, 2019: Microscopy and Microanalysis
Clifford S Todd, William A Heeschen, Peter Y Eastman, Ellen C Keene
Silver nanowire (AgNW) diameters are typically characterized by manual measurement from high magnification electron microscope images. Measurement is monotonous and has potential ergonomic hazards. Because of this, statistics regarding wire diameter distribution can be poor, costly, and low-throughput. In addition, manual measurements are of unknown uncertainty and operator bias. In this paper we report an improved microscopy method for diameter and yield measurement of nanowires in terms of speed/automation and reduction of analyst variability...
February 13, 2019: Microscopy and Microanalysis
Jiong Chen, Wei Feng, Fei Zhan
Human lipocalin 6 (hLCN6) is an epididymis-specific secretory protein. It binds to sperm and plays important role in sperm maturation. To explore the feasibility for isolating spermatozoa from mixed cells using anti-hLCN6 monoclonal antibody-conjugated immunomagnetic beads (anti-hLCN6 IMBs) and establish a new method for the separation of sperms from mixed stains, 2 sets of 30 cases of cell mixture suspensions and stains containing different proportions of sperm and epithelial cells were prepared. Biotin-labeled anti-hLCN6 monoclonal antibody (mAb) was incubated with the cell mixtures, and the spermatozoa were then isolated with avidin-coated IMBs...
January 25, 2019: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
N Akhter, Z Sultana, A Bhattacharjee
Genitourinary fistula is a psychophysiosocial distress that results in prolonged labour. Gradually, it has become a public health issue globally with advancing of its incidence day by day. A handsome number of mothers are also suffering in Bangladesh from genitourinary fistula. This study was done to observe the patterns of presentations of genitourinary fistula and their fetal outcome. One hundred sixteen (116) consecutive patients were enrolled in the study by purposive sampling as a diagnosed case of genitourinary fistula presented in Department of Obstetrics & Gynecology of Dhaka Medical College Hospital, Dhaka, Bangladesh from January 2017 to September 2017...
January 2019: Mymensingh Medical Journal: MMJ
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