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Neuropathic pain rats

Víctor M López-Álvarez, Stefano Cobianchi, Xavier Navarro
OBJECTIVES: We aimed to investigate if different protocols of electrical stimulation following nerve injury might improve neuropathic pain outcomes and modify associated plastic changes at the spinal cord level. MATERIALS AND METHODS: Adult rats were subjected to sciatic nerve transection and repair, and distributed in four groups: untreated (SNTR, n = 12), repeated acute electrical stimulation (rAES, 50 Hz, one hour, n = 12), chronic electrical stimulation (CES, 50 Hz, one hour, n = 12), and increasing-frequency chronic electrical stimulation (iCES, one hour, n = 12) delivered during two weeks following the lesion...
February 20, 2019: Neuromodulation: Journal of the International Neuromodulation Society
Laura Micheli, Carla Ghelardini, Elena Lucarini, Carmen Parisio, Elena Trallori, Lorenzo Cinci, Lorenzo Di Cesare Mannelli
OBJECTIVES: The creation of a new valid preclinical model of articular pain by the intra-articular (i.a.) injection of mucilages for the screening of new treatments against arthritis. METHODS: A single intra-articular injection (20 μl) of mucilages (from Althaea officinalis roots and Linum usitatissimun seeds) or vegetal components (Amorphophallus konjac gum powder and β-glucan, used as reference standard) were assessed in the rat. The pathology progression was monitored by behavioural measurements (paw pressure test, von Frey test, incapacitance test and beam balance test) and compared to that induced by the i...
February 19, 2019: Journal of Pharmacy and Pharmacology
Kimberly E Stephens, Weiqiang Zhou, Zhicheng Ji, Zhiyong Chen, Shaoqiu He, Hongkai Ji, Yun Guan, Sean D Taverna
BACKGROUND: Pain is a subjective experience derived from complex interactions among biological, environmental, and psychosocial pathways. Sex differences in pain sensitivity and chronic pain prevalence are well established. However, the molecular basis underlying these sex dimorphisms are poorly understood particularly with regard to the role of the peripheral nervous system. Here we sought to identify shared and distinct gene networks functioning in the peripheral nervous systems that may contribute to sex differences of pain in rats after nerve injury...
February 19, 2019: BMC Genomics
Yong-Da Liu, Zhi-Bin Wang, Guang Han, Li Jin, Ping Zhao
Background: Our previous study suggested that HBO treatment attenuated neuropathic pain by inhibiting mTOR to induce autophagy in SNL neuropathic pain model. The aim of this study was to evaluate the role of AKT/TSC2/mTOR pathway in SNL and autophagy and determine whether HBO treatment could relieve neuropathic pain via modulating AKT/TSC2/mTOR pathway. Materials and methods: Rats were randomly divided into sham, SNL, SNL + HBO treatment, SNL + vehicle, and SNL + AKT inhibitor groups...
2019: Journal of Pain Research
Wei Xiong, Mengxia Tan, Zhoujie Tong, Cancan Yin, Lingkun He, Lijuan Liu, Yulin Shen, Shu Guan, Huixiang Ge, Guodong Li, Shangdong Liang, Yun Gao
Trigeminal neuralgia (TN) is the most common neuropathic pain in the facial area, for which the effective therapy is unavailable. Long non-coding RNA (lncRNA) such as lncRNA uc.48+ is involved in diabetic neuropathic pain and may affect purinergic signaling in ganglia of diabetic rats. In this research, chronic constriction injury of the infraorbital nerve (CCI-ION) was applied to establish a rat model of TN. Five days after local injection of siRNA targeting the lncRNA uc.48+ in trigeminal ganglia (TGs), the upregulated uc...
February 14, 2019: Brain Research Bulletin
Kensuke Saeki, Shun-Ichi Yasuda, Masami Kato, Mayumi Kano, Yuki Domon, Naohisa Arakawa, Yutaka Kitano
Mirogabalin, a novel ligand for the α2 δ subunit of voltage-gated calcium channels, is under the development for the treatment of neuropathic pain. Mirogabalin specifically and potently binds to α2 δ subunits, and it shows analgesic effects in both peripheral and central neuropathic pain models in rats. To expand pharmacological findings on mirogabalin and provide additional information of its potential for chronic pain therapy, we examined the effects of mirogabalin in 2 experimental models of fibromyalgia, namely, the intermittent cold stress model (ICS model) and the unilateral intramuscular acidic saline injection model (Sluka model)...
February 15, 2019: Naunyn-Schmiedeberg's Archives of Pharmacology
Mei Yee Kwan, Anthony Choo, Taleen Hanania, Afshin Ghavami, Jose Beltran, John Shea, Amidi Barboza, Andrew Hu, Marcie Fowler, Venugopal Rao Neelagiri, Irving Sucholeiki
There is an unmet medical need for the development of non-addicting pain therapeutics with enhanced efficacy and tolerability. The current study examined the effects of AQU-118, an orally active inhibitor of metalloproteinase-2 (MMP-2) and MMP-9, in the spinal nerve ligation (SNL) rat model of neuropathic pain. Mechanical allodynia and the levels of various biomarkers were examined within the dorsal root ganglion (DRG) before and after oral dosing with AQU-118. The rats that received the SNL surgery exhibited significant mechanical allodynia as compared to sham controls...
February 14, 2019: International Journal of Molecular Sciences
Qin-Yi Chen, Chao-Yang Tan, Yang Wang, Ke-Tao Ma, Li Li, Jun-Qiang Si
Transmembrane member 16A (TMEM16A) is involved in many physiological functions, such as epithelial secretion, sensory conduction, nociception, control of neuronal excitability, and regulation of smooth muscle contraction, and may be important in peripheral pain transmission. To explore the role of TMEM16A in the persistent hyperalgesia that results from chronic constriction injury-induced neuropathic pain, a rat model of the condition was established by ligating the left sciatic nerve. A TMEM16A selective antagonist (10 μg T16Ainh-A01) was intrathecally injected at L5-6...
June 2019: Neural Regeneration Research
Wenjun Zhang, Yuguo Liu, Yi Sun, Zengxu Liu
OBJECTIVES: The aim of this study was to determine the role of microencapsulated olfactory ensheathing cell (MC-OEC) transplantation in rats with sciatic nerve injury-induced pain, and its relationship with P2 × 7 receptor expression in the L4-5 spinal cord segment. METHODS: Olfactory bulb tissues of healthy Sprague Dawley (SD) rats were collected to culture olfactory ensheathing cells using differential attachment methods. Forty-eight healthy SD rats were randomly assigned to four groups: the sham, chronic constriction injury (CCI), olfactory ensheathing cell (OEC), and MC-OEC groups...
February 9, 2019: Neuroscience Letters
Guadalupe García, Erika J Gutiérrez-Lara, David Centurión, Vinicio Granados-Soto, Janet Murbartián
Peripheral neuropathy is one of the main complications of diabetes. The pathogenesis of this affectation is not completely understood. Several studies refer to hyperglycemia as the principal cause of diabetic neuropathy. Nonetheless, there are changes in the expression of insulin receptor during the progress of diabetic neuropathy, suggesting that this disorder begins before high glucose blood levels are established. In this study, we investigated fructose-induced insulin resistance as a model of neuropathic pain...
February 8, 2019: Neuroscience
Xiao-Min Zhang, Ling-Zhi Wang, Bo He, Yu-Ke Xiang, Li-Xia Fan, Qin Wang, Liang Tao
Gap junctions (GJs) are novel molecular targets for pain therapeutics due to their pain-promoting function. INI-0602, a new GJ inhibitor, exerts a neuroprotective role, while its role in neuropathic pain is unclear. The objective was to investigate the analgesic role and mechanisms of INI-0602 in neuropathic pain induced by spared nerve injury (SNI), and whether INI-0602 attenuated pain-induced depression-like behaviors. Rats were randomly assigned to saline treatment groups (sham+NS and SNI+NS) or INI-0602 treatment groups (sham+INI-0602 and SNI+INI-0602)...
February 7, 2019: Neuroreport
Yangyi Li, Chengbo Chen, Shunyuan Li, Changcheng Jiang
The aim of this study was to investigate whether ginsenoside Rf can effectively relieve pain hypersensitivity in a neuropathic pain rat model. Neuropathic pain was induced in rats by chronic constriction injury (CCI) to the right sciatic nerve. Ginsenoside Rf was administered intraperitoneally after CCI surgery. The von Frey filament test and forced swimming test were performed to examine pain hypersensitivity and depression-like behavior in rats. Western blot was used to measure the levels of inflammatory cytokines in the dorsal root ganglion (DRG) and the spinal cord...
February 11, 2019: Phytotherapy Research: PTR
Zhao-Ling Chen, Jing-Yi Liu, Fang Wang, Xu Jing
Long noncoding RNAs (lncRNAs) are involved in multiple nervous system diseases, including neuropathic pain. Previous studies have demonstrated that lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) has been identified as a diagnostic biomarker in many diseases. Nevertheless, the function of MALAT1 in neuropathic pain progression is still unclear. Here, we established a chronic constriction injury (CCI) rat model. We found that MALAT1 was remarkably upregulated in CCI rats. In addition, neuropathic pain behaviors such as mechanical and thermal hyperalgesia were reduced by the inhibition of MALAT1...
February 10, 2019: Journal of Cellular Physiology
Jun Shen, Bei-Bei Huo, Xu-Yun Hua, Mou-Xiong Zheng, Ye-Chen Lu, Jia-Jia Wu, Chun-Lei Shan, Jian-Guang Xu
The present study aimed to investigate cerebral metabolic changes in a neuropathic pain model following deafferentation. A total of 24 Sprague-Dawley rats were included for modeling of right brachial plexus avulsion (BPA) through the posterior approach. As nerve injury would cause central sensitization and facilitate pain sensitivity in other parts of the body, thermal withdrawal latency (TWL) of the intact forepaw was assessed to investigate the level of pain perception following BPA-induced neuropathic pain...
February 6, 2019: Brain Research
Jose Antonio Lopez Garcia
In this issue, you will find a paper by Vasović et al., entitled "Trigeminal neuropathic pain development and maintenance in rats are suppressed by a positive modulator of α6GABAA receptors" (D. Vasović et al., 2019). Loss of GABA-mediated inhibition within pain circuits is one of the mechanisms commonly invoked to explain allodynia and hyperalgesia following injury. Intrathecal administration of picrotoxin produces pain like signs. Positive allosteric modulators of GABAA receptors like benzodiazepines are known to produce analgesia although their main effects are sedative, hypnotic and anxiolytic and therefore their use as analgesics is curtailed (Schliessbach et al...
February 8, 2019: European Journal of Pain: EJP
Jose Eduardo Hernandez-Reyes, Ana Belen Salinas-Abarca, Guadalupe C Vidal-Cantu, Guadalupe Raya-Tafolla, David Elias-Viñas, Vinicio Granados-Soto, Rodolfo Delgado-Lezama
Diabetic neuropathy is an incapacitating complication in diabetic patients. The cellular and molecular mechanisms involved in this pathology are poorly understood. Previous studies have suggested that the loss of spinal GABAergic inhibition participate in painful diabetic neuropathy. However, the role of extrasynaptic α5 subunit-containing GABAA (α5GABAA) receptors in this process is not known. The purpose of this study was to investigate the role of α5GABAA receptors in diabetes-induced tactile allodynia, loss of rate dependent depression (RDD) of the Hoffmann reflex (HR) and modulation of primary afferent excitability...
January 31, 2019: Pain
Xijie Zhou, Bin Zhao, Keshav Poonit, Weidong Weng, Chenglun Yao, Chao Sun, Hede Yan
OBJECTIVETraumatic neuromas represent a prevalent source of neuropathic pain. As of yet, there has been no single treatment method that can guarantee permanent relief of symptoms. Although nerve-capping techniques have shown promise, their exact mechanisms remain elusive. The authors' aim was to examine the role of the RhoA/ROCK signaling pathway in the prevention of neuroma formation after neurectomy utilizing a nerve-capping technique.METHODSAn aligned nanofiber tube was fabricated to cap the sciatic nerve in Sprague Dawley rats...
February 1, 2019: Journal of Neurosurgery
Bahardokht Tolou-Dabbaghian, Ladan Delphi, Ameneh Rezayof
The present study investigated the role of the amygdala NMDA receptors/NOS pathway in morphine-induced anti-allodynia. Concurrently with the bilateral cannulation of the central amygdala (CeA), chronic constriction of the sciatic nerve was performed on male Wistar rats. Morphine (3-5 mg/kg) was intraperitoneally administered to induce anti-allodynia. When D-AP5, a selective NMDA receptor antagonist, (0.05-0.1 µg/rat) or L-NAME, the NO synthase inhibitor, (0.1-0.5 µg/rat) were microinjected into the CeA, the higher doses potentiated an ineffective dose of morphine (3 mg/kg)...
January 29, 2019: Journal of Pain: Official Journal of the American Pain Society
Chun Yang, Xi Fang, Gaofeng Zhan, Niannian Huang, Shan Li, Jiangjiang Bi, Riyue Jiang, Ling Yang, Liying Miao, Bin Zhu, Ailin Luo, Kenji Hashimoto
Patients with chronic neuropathic pain frequently suffer from symptoms of anhedonia, which is a core symptom of depression. Accumulating studies suggest that gut microbiota may play a role in depression via gut-microbiota-brain axis. However, it is unknown whether gut microbiota plays a role in neuropathic pain-associated anhedonia. Here, we used a rat model of spared nerve injury (SNI). Hierarchical cluster analysis of sucrose preference test (SPT) results was used to classify the SNI rats with or without anhedonia-like phenotype...
January 31, 2019: Translational Psychiatry
Peipei Wu, Yong Chen
Chemotherapy-induced neuropathic pain (CINP) is a common and debilitating side effect of cancer treatment. Evodiamine, a major effective compound isolated from Evodia rutaecarpa, has been associated with anti-inflammatory and anti-nociceptive effects, an important therapeutic strategy for the treatment of neuropathic pain. However, the effects of evodiamine on CINP remain unknown. Thus, this study aims to investigate the pharmacological potential of evodiamine in attenuating paclitaxel-induced peripheral neuropathy...
January 30, 2019: Human Cell
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