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Burn mice

Lei Zhao, Kai Wang, Weichao Li, Thanapop Soteyome, Hang Xiao, Zhuoyan Hu
The objective of this study was to evaluate the ability of a polyphenolic extract from longan seeds as a wound-healing material for deep second-degree burn wounds. The total phenol content of the extract reached 45.28 ± 1.22 mg GAE per g DW when 70% ethanol was used as the extraction solvent, and then AB-8 resin was used for purification. Results from the antioxidant assay in vitro (DPPH˙ and hydroxyl radical scavenging activity) and antimicrobial activity assay (ZOI, MIC and MBC) showed that the fractions eluted by 30% ethanol tend to have greater antioxidant ability, as well as higher bactericidal and antibacterial activities, than the other elution fractions...
February 15, 2019: Food & Function
Yasuhiko Kaita, Takehiko Tarui, Hideaki Yoshino, Takeaki Matsuda, Yoshihiro Yamaguchi, Takatoshi Nakagawa, Michio Asahi, Masaaki Ii
Introduction: The purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs. Methods: Full thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses...
June 2019: Regenerative Therapy
Blaine Phillips, Justyna Szostak, Bjoern Titz, Walter K Schlage, Emmanuel Guedj, Patrice Leroy, Gregory Vuillaume, Florian Martin, Ansgar Buettner, Ashraf Elamin, Alain Sewer, Nicolas Sierro, Mohamed Amin Choukrallah, Thomas Schneider, Nikolai V Ivanov, Charles Teng, Ching Keong Tung, Wei Ting Lim, Ying Shan Yeo, Patrick Vanscheeuwijck, Manuel C Peitsch, Julia Hoeng
Smoking is one of the major modifiable risk factors in the development and progression of chronic obstructive pulmonary disease (COPD) and cardiovascular disease (CVD). Modified-risk tobacco products (MRTP) are being developed to provide substitute products for smokers who are unable or unwilling to quit, to lessen the smoking-related health risks. In this study, the ApoE-/- mouse model was used to investigate the impact of cigarette smoke (CS) from the reference cigarette 3R4F, or aerosol from two potential MRTPs based on the heat-not-burn principle, carbon-heated tobacco product 1...
February 11, 2019: Food and Chemical Toxicology
Harumasa Nakazawa, Kazuhiro Ikeda, Shohei Shinozaki, Shingo Yasuhara, Yong-Ming Yu, J A Jeevendra Martyn, Ronald G Tompkins, Tomoko Yorozu, Satoshi Inoue, Masao Kaneki
Mitochondrial dysfunction is associated with metabolic alterations in various disease states, including major trauma (e.g., burn injury). Metabolic derangements, including muscle insulin resistance and hyperlactatemia, are a clinically significant complication of major trauma. Coenzyme Q10 (CoQ10) is an essential cofactor for mitochondrial electron transport, and its reduced form acts as a lipophilic antioxidant. Here, we report that burn injury induces impaired muscle insulin signaling, hyperlactatemia, mitochondrial dysfunction (as indicated by suppressed mitochondrial oxygen consumption rates), morphological alterations of the mitochondria (e...
February 2019: FEBS Open Bio
Shuang Long, Guojian Wang, Mingqiang Shen, Na Zhao, Huimin Wan, Yang Xu, Song Wang, Cheng Wang, Jining Gao, Yuhui Hao, Aiping Wang, Rong Li, Xinze Ran, Yongping Su, Junping Wang, Tao Wang
Exposure to ionizing radiation combined with traumatic tissue injury is an important life-threatening condition found in the civilian populations after nuclear and radiological events. The significance feature of radiation combined injury (RCI) is the severe combined effect, which makes the injury more complicated. At present, there are limited measures available to treat RCI. Here we show that a chimeric protein dTMP-GH, fusing human growth hormone (hGH) with a tandem dimer of thrombopoietin mimetic peptide (dTMP), could be an effective therapy agent for RCI in a mice model...
February 13, 2019: Radiation Research
Samantha Teng, Lipi Chakravorty, Naomi Fleury, Shelley Gorman
OBJECTIVE: Obesity often emerges in middle age, increasing risk for metabolic disorders. Our previous preclinical experiments identified that chronic exposure to non-burning ultraviolet radiation, like that achieved through sun exposure, prevented weight gain and signs of metabolic dysfunction in young adult mice fed a high fat diet. Our objective was to perform a pilot study to estimate the effect size of ongoing exposure to sub-erythemal (non-burning, low dose) UVB (1 kJ/m2 ) radiation on measures of adiposity, food intake and physical activity in 'mature' adult C57Bl/6J male mice fed a high fat diet for 12 weeks...
February 11, 2019: BMC Research Notes
Kyung Eun Han, Mi Hye Park, Kyoung Hye Kong, Euno Choi, Kyu-Ryong Choi, Roo Min Jun
PURPOSE: To compare the therapeutic effects of human derivatives in a mouse alkali burn model. METHODS: The right eyes of mice were injured using NaOH. After alkali injury, one of the following agents was topically administered for 7 days: human amniotic membrane (hAM) suspension, human umbilical cord serum (hUCS), and human peripheral blood serum (hPBS), or saline. The epithelial defect areas on days 1, 2, and 3, degrees of opacity on days 2, 3, and 7, and corneal neovascularization (NV) areas on day 7 were evaluated...
February 10, 2019: Cutaneous and Ocular Toxicology
Ana M Jaramillo, Lucia Piccotti, Walter V Velasco, Anna Sofia Huerta Delgado, Zoulikha Azzegagh, Felicity S Chung, Usman I Nazeer, Junaid Farooq, Joshua M Brenner, Jan Parker-Thornburg, Brenton L Scott, Christopher M Evans, Roberto Adachi, Alan R Burns, Silvia M Kreda, Michael J Tuvim, Burton F Dickey
Airway mucin secretion is necessary for ciliary clearance of inhaled particles and pathogens, but can be detrimental in pathologies such as asthma and cystic fibrosis. Exocytosis in mammals requires a Munc18 scaffolding protein, and airway secretory cells express all three Munc18 isoforms. Using conditional airway epithelial deletant mice, we found that Munc18a has the major role in baseline mucin secretion, Munc18b has the major role in stimulated mucin secretion, and Munc18c does not function in mucin secretion...
February 5, 2019: JCI Insight
Md Mamun Al-Amin, Robert K P Sullivan, Nyoman D Kurniawan, Thomas H J Burne
Converging evidence from human and animal studies support an association between vitamin D deficiency and cognitive impairment. Previous studies have shown that hippocampal volume is reduced in adults with vitamin D deficiency as well as in a range of disorders, such as schizophrenia. The aim of the current study was to examine the effect of adult vitamin D (AVD) deficiency on hippocampal-dependent spatial learning, and hippocampal volume and connectivity in healthy adult mice. Ten-week-old male BALB/c mice were fed a control (vitamin D 1500 IU/kg) or vitamin D-depleted (vitamin D 0 IU/kg) diet for a minimum of 10 weeks...
February 2, 2019: Brain Structure & Function
Li Ma, Yinhui Zhou, Mohammed A S Khan, Shingo Yasuhara, J A Jeevendra Martyn
INTRODUCTION: Burn injury (BI) leads to both systemic and neuro-inflammation and is associated with muscle wasting and weakness, which increase morbidity and mortality. Disuse atrophy is concomitantly present in BI patients. Most studies have focused on muscle with little attention to role of central nervous system (CNS) in the neuromuscular changes. We tested the hypothesis that BI-induced muscle wasting stems from CNS microglia activation and cytokines and chemokine release, that is associated with spinal ventral horn motor neuron degeneration...
December 6, 2018: Shock
Laura D Gamble, Stefania Purgato, Jayne Murray, Lin Xiao, Denise M T Yu, Kimberley M Hanssen, Federico M Giorgi, Daniel R Carter, Andrew J Gifford, Emanuele Valli, Giorgio Milazzo, Alvin Kamili, Chelsea Mayoh, Bing Liu, Georgina Eden, Sara Sarraf, Sophie Allan, Simone Di Giacomo, Claudia L Flemming, Amanda J Russell, Belamy B Cheung, Andre Oberthuer, Wendy B London, Matthias Fischer, Toby N Trahair, Jamie I Fletcher, Glenn M Marshall, David S Ziegler, Michael D Hogarty, Mark R Burns, Giovanni Perini, Murray D Norris, Michelle Haber
Amplification of the MYCN oncogene is associated with an aggressive phenotype and poor outcome in childhood neuroblastoma. Polyamines are highly regulated essential cations that are frequently elevated in cancer cells, and the rate-limiting enzyme in polyamine synthesis, ornithine decarboxylase 1 (ODC1), is a direct transcriptional target of MYCN. Treatment of neuroblastoma cells with the ODC1 inhibitor difluoromethylornithine (DFMO), although a promising therapeutic strategy, is only partially effective at impeding neuroblastoma cell growth due to activation of compensatory mechanisms resulting in increased polyamine uptake from the surrounding microenvironment...
January 30, 2019: Science Translational Medicine
Eduardo I Cardenas, Ricardo Gonzalez, Keegan Breaux, Qi Da, Berenice A Gutierrez, Marco A Ramos, Rodolfo A Cardenas, Alan R Burns, Rolando E Rumbaut, Roberto Adachi
Platelet degranulation, a form of regulated exocytosis, is crucial for hemostasis and thrombosis. Exocytosis in platelets is mediated by SNARE proteins, and in most mammalian cells this process is controlled by Munc18 (mammalian homolog of C. elegans uncoordinated gene 18) proteins. Platelets express all Munc18 paralogs (Munc18‑1, ‑2, and ‑3), but their roles in platelet secretion and function have not been fully characterized. Using Munc18‑1, ‑2, and ‑3 conditional knockout mice, here we deleted expression of these proteins in platelets and assessed granule exocytosis...
January 29, 2019: Journal of Biological Chemistry
Zabeen Lateef, Gabriella Stuart, Nicola Jones, Andrew Mercer, Stephen Fleming, Lyn Wise
Many burn interventions aim to target the inflammatory response as a means of enhancing healing or limiting hypertrophic scarring. Murine models of human burns have been developed, but the inflammatory response to injury in these models has not been well defined. The aim of this study was to profile inflammatory cell populations and gene expression relative to healing and scarring in a murine model of thermal burns. Cutaneous injuries were created on the dorsal region of C57Bl/6 mice using a heated metal rod...
January 28, 2019: International Journal of Molecular Sciences
Rebecca L Jones, Stephanie A Lang, Jessica A Kendziorski, Alexis D Greene, Katherine A Burns
BACKGROUND: Endometriosis is a gynecological disease affecting 1 in 10 women of reproductive age. Endometriosis incidence has risen; however, whether this rise is due to disease awareness or environmental contamination is not known. OBJECTIVE: The objective of this study was to determine if bisphenol A (BPA) or bisphenol AF (BPAF) potentiate the development of endometriosis and if hormonal status alters how toxicant exposure affects disease. METHODS: A mouse model of endometriosis, where minced uterine tissue is injected into the peritoneal cavity of a host mouse, was used to examine the effects of BPA and BPAF on endometriosis lesion development in ovariectomized and hormonally intact mice...
December 2018: Environmental Health Perspectives
Elizabeth G Wheatley, Brenda J Curtis, Holly J Hulsebus, Devin M Boe, Kevin Najarro, Diana Ir, Charles E Robertson, Mashkoor A Choudhry, Daniel N Frank, Elizabeth J Kovacs
Maintenance of the commensal bacteria that comprise the gut microbiome is essential to both gut and systemic health. Traumatic injury, such as burn, elicits a number of changes in the gut, including a shift in the composition of the microbiome (dysbiosis), increased gut leakiness, and bacterial translocation into the lymphatic system and bloodstream. These effects are believed to contribute to devastating secondary complications following burn, including pneumonia, acute respiratory distress syndrome, multi-organ failure, and septic shock...
January 18, 2019: Shock
Min-Ah Koo, Seung Hee Hong, Mi Hee Lee, Byeong-Ju Kwon, Gyeung Mi Seon, Min Sung Kim, Dohyun Kim, Ki Chang Nam, Jong-Chul Park
Extensive skin loss caused by burns or diabetic ulcers may lead to major disability or even death. Therefore, cell-based therapies that enhance skin regeneration are clinically needed. Previous approaches have been applied the injections of cell suspensions and the implantation of biodegradable three-dimensional scaffolds seeded cells. However, these treatments have limits due to poor localization of the injected cells and insufficient delivery of oxygen and nutrients to cells. Recently, cell sheet-based tissue engineering has been developed to transplant cell sheets, which are cell-dense tissues without scaffolds...
January 16, 2019: Acta Biomaterialia
Christoph Wallner, Julika Huber, Marius Drysch, Sonja V Schmidt, Johannes Maximilian Wagner, Mehran Dadras, Stephanie Dittfeld, Mustafa Becerikli, Henriette Jaurich, Marcus Lehnhardt, Björn Behr
Tumors, traumata, burn injuries or surgeries can lead to critical-sized bony defects which need to be reconstructed. Mesenchymal stem cells (MSCs) have the ability to differentiate into multiple cell lineages and thus present a promising alternative for use in tissue engineering and reconstruction. However there is an ongoing debate whether all MSCs are equivalent in their differentiation and proliferation ability. The goal of this study was to asses osteogenic and adipogenic characteristic changes of adipose-derived stem cells (ASCs) and bone marrow-derived stem cells (BMSCs) upon Myostatin inhibition with Follistatin...
January 18, 2019: Stem Cells and Development
Akbar Ahmad, Nadiya Druzhyna, Csaba Szabo
The gaseous transmitter hydrogen sulfide (H2S) has been implicated in various forms of critical illness. Here we have compared the outcome of scald burn injury in wild-type mice and in mice deficient in 3-mercaptopyruvate sulfurtransferase (3-MST), a mammalian H2S-generating enzyme. Outcome variables included indices of organ injury, clinical chemistry parameters and plasma levels of inflammatory mediators. Plasma levels of H2S significantly increased in response to burn in wild-type mice, but remained unchanged in 3-MST-/- mice...
January 12, 2019: Journal of Burn Care & Research: Official Publication of the American Burn Association
Avijit Ray, Mohamed I Khalil, Kirthi L Pulakanti, Robert T Burns, Cody J Gurski, Sreemanti Basu, Demin Wang, Sridhar Rao, Bonnie N Dittel
A number of different B cell subsets have been shown to exhibit regulatory activity using a variety of mechanisms to attenuate inflammatory diseases. Here we show, using anti-CD20-mediated partial B cell depletion in mice, that a population of mature B cells distinguishable by IgDlow/- expression maintains tolerance by, at least in part, promoting CD4+ Foxp3+ regulatory T cell homeostatic expansion via glucocorticoid-induced tumor necrosis factor receptor ligand, or GITRL. Cell surface phenotyping, transcriptome analysis and developmental study data show that B cells expressing IgD at a low level (BDL ) are a novel population of mature B cells that emerge in the spleen from the transitional-2 stage paralleling the differentiation of follicular B cells...
January 14, 2019: Nature Communications
Junnan Chen, Linying Lai, Kui Ma, Xiao Xu, Zhenya Huang, Guiwen Zhou, Yunchao Zhou, Liming Liang, Minliang Chen
BACKGROUND: Chyle fat transplantation has shown positive effects on preexisting human hypertrophic scars (HSs) in a nude mouse HS graft model. METHODS: Hypertrophic scar fragments were obtained from 5 surgically treated burn patients and implanted into the backs of nude mice in 3 groups: group A, control; group B, triamcinolone; and group C, chyle fat. The specimens were implanted after the corresponding intralesional injection in each group, and the mice were observed for 4 weeks...
January 9, 2019: Annals of Plastic Surgery
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