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Colitis rats

Atsarina Larasati Anindya, Risa Dwi Oktaviani, Benita Rachel Praevina, Sophi Damayanti, Neng Fisheri Kurniati, Catur Riani, Heni Rachmawati
We have successfully conjugated mesalamine (5-aminosalicylic acid, 5-ASA) with xylan, a biopolymer isolated from pineapple stem waste, to form xylan-5-ASA conjugate. The biopolymer was used to provide colon-targeting properties for 5-ASA, a golden standard anti-inflammatory agent commonly used for ulcerative colitis treatment. A series of data from FTIR spectroscopy, UV-Vis spectrophotometry, and HPLC confirmed the xylan-5-ASA conjugate formation. To ensure successful colon targeting properties, in vitro and in vivo drug release studies after oral administration of xylan-5-ASA conjugate to Wistar rats were performed...
February 13, 2019: AAPS PharmSciTech
Lei Zhu, PeiQing Gu, Hong Shen
BACKGROUND: Berberine hydrochloride is one the effective compound among Rhizoma Coptidis, Cortex Phellodendri, and other plants. There are several clinical functions of berberine hydrochloride including anti-inflammation, antitumor and immunoregulatory. However, the anti-inflammatory of berberine hydrochloride in ulcerative colitis is barely understood. In this study, we aimed to explore the effects of berberine hydrochloride on dextran sulfate sodium (DSS)-induced rats model of ulcerative colitis...
February 8, 2019: International Immunopharmacology
Emmeline Salameh, Mathieu Meleine, Guillaume Gourcerol, Jean-Claude do Rego, Jean-Luc do Rego, Romain Legrand, Jonathan Breton, Moutaz Aziz, Charlène Guérin, Moïse Coeffier, Guillaume Savoye, Rachel Marion-Letellier
BACKGROUND: Inflammatory bowel diseases (IBD) are characterized by flares repetition and remission periods leading to chronic post-inflammatory sequels. Among them, one third of IBD patients are suffering from persisting functional symptoms or psychological comorbidities during remission. Our study aimed to assess functional and behavioral sequels of chronic colitis in rats with quiescent intestinal inflammation. METHODS: Chronic colitis was induced by a weekly intrarectal injection of TNBS for 3 weeks in 30 rats while control rats (n=24) received the vehicle...
February 8, 2019: American Journal of Physiology. Gastrointestinal and Liver Physiology
Go Yasuda, Hiromu Ito, Hiromi Kurokawa, Masahiko Terasaki, Hideo Suzuki, Yuji Mizokami, Hirofumi Matsui
The Chinese herbal medicine Qing Dai has been traditionally used for the treatment of various inflammatory diseases. We previously reported that reactive oxygen species play an important role in bisphosphonate-induced gastrointestinal injuries and that Qing Dai improved ulcerative colitis by scavenging reactive oxygen species. In this study, we investigated whether Qing Dai prevented bisphosphonate-induced gastric cellular injuries. Risedronate (a bisphosphonate) was added to rat gastric mucosal cells. Risedronate-induced cellular injury, cellular lipid peroxidation, mitochondrial membrane potential, and reactive oxygen species production in rat gastric mucosal cells were examined via viable cell counting, specific fluorescent indicators, and electron spin resonance...
January 2019: Journal of Clinical Biochemistry and Nutrition
Yuzhen Hou, Hui Wang, Fan Zhang, Fengyuan Sun, Meng Xin, Mengshuang Li, Jun Li, Xianggen Wu
Purpose: Rebaudioside A (RA) has nanocarrier characteristics that allow it to self-assemble into micelles in aqueous solutions. The purpose of this study was to determine if a self-nanomicellizing solid dispersion based on RA could be utilized as an oral nano-drug delivery system. Materials and methods: Curcumin (Cur) served as a model hydrophobic drug, and a Cur-loaded self-nanomicellizing solid dispersion based on RA (RA-Cur) was formulated. The properties of RA-Cur in the solid state and in aqueous solution were characterized...
2019: International Journal of Nanomedicine
Hui Wu, Heng Fan, Zhexing Shou, Meng Xu, Qianyun Chen, Changzheng Ai, Yalan Dong, Yujin Liu, Zhen Nan, Yifan Wang, Ting Yu, Xingxing Liu
Accumulating evidence indicates that microRNA-146a (miR-146a), a well-known anti-inflammatory miRNA, acts as a negative feedback regulator of the innate immune response, but its role in modulation of inflammatory bowel disease (IBD) remains unclear and the issue related to the stability of exogenous miR-146a in blood is up in the air. In this study, extracellular vesicles (EVs) from cultured medium of bone-marrow mesenchymal stem cells (BMSCs) transfected with recombinant lentiviruses can serve as a stable delivery system and overexpress miR-146a, which significantly inhibited TNF receptor-associated factor 6 (TRAF6) and IL-1 receptor-associated kinase 1 (IRAK1) expression in TNBS-induced colitis of rats...
January 14, 2019: International Immunopharmacology
Yejin Yang, Wooseong Kim, Dayoon Kim, Seongkeun Jeong, Jin-Wook Yoo, Yunjin Jung
Background: We examined whether metoclopramide (MCP), a modulator of dopamine and serotonin receptors, alleviated colitis and had synergistic effects when coadministered with 5-aminosalicylic acid (5-ASA) in an experimental model of colitis. Methods: MCP azo-linked to 5-ASA (5-[4-chloro-2-{2-(diethylamino)ethylcarbamoyl}- 1-methoxyphenyl]azosalicylic acid, MCP-azo-ASA) was synthesized, where 5-ASA was used as a colon-targeting carrier and an anti-colitic agent, and the ability of MCP-azo-ASA to target the colon in vitro and in vivo was evaluated...
2019: Drug Design, Development and Therapy
Alba Rodríguez-Nogales, Francesca Algieri, Teresa Vezza, José Garrido-Mesa, José Alberto Molina-Tijeras, María Elena Rodríguez-Cabezas, María Pilar Utrilla, Ivo Pischel, Julio Gálvez
Pyruvate is a normal constituent of the body that participates in carbohydrate metabolism and functions as a scavenger of free radicals. Calcium pyruvate monohydrate (CPM) is a more stable derivative that has proved its anti-inflammatory effect in experimental colitis, among other disorders, and that could also be considered a source of calcium. Thus, it would be useful for the treatment of diseases with an inflammatory component and a high prevalence of osteoporosis like the irritable bowel syndrome (IBS)...
January 10, 2019: Nutrients
Gehan El-Akabawy, Neveen M El-Sherif
Ulcerative colitis is a common intestinal inflammatory disease characterized by upregulation of pro-inflammatory cytokines and oxidative stress. Zeaxanthin is a nutritional carotenoid that belongs to the xanthophyll family of pigments. It exerts potent anti-inflammatory and antioxidative effects. The present study aimed to evaluate the effect of zeaxanthin on acetic acid-induced ulcerative colitis in rats. Rats were randomly categorized into five groups: control, zeaxanthin, acetic acid, acetic acid + zeaxanthin, and acetic acid + prednisolone groups...
January 4, 2019: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Sameh Saber, Rania M Khalil, Walied S Abdo, Doaa Nassif, Eman El-Ahwany
Alteration in the expression pattern of Nrf-2 and NFκB has been reported in ulcerative colitis (UC) in which functional crosstalk between these two critical pathways has been suggested. The ameliorative potential of the AT1R blocker olmesartan (OLM) on oxidative stress and inflammatory cytokines has received considerable attention in recent years. Acetic acid (AA)-induced UC demonstrates close resemblance to human UC regarding histopathological features and cytokine profile and is associated with local intense immune response, oxidative stress and release of inflammatory cytokines...
December 27, 2018: Toxicology and Applied Pharmacology
Panagiotis Papatheodorou, Shuo Song, Diana López-Ureña, Alexander Witte, Felícia Marques, Gerhard Stefan Ost, Björn Schorch, Esteban Chaves-Olarte, Klaus Aktories
Clostridium difficile is associated with antibiotic-associated diarrhea and pseudomembranous colitis in humans. Its 2 major toxins, toxins A and B, enter host cells and inactivate GTPases of the Ras homologue/rat sarcoma family by glucosylation. Pore formation of the toxins in the endosomal membrane enables the translocation of their glucosyltransferase domain into the cytosol, and membrane cholesterol is crucial for this process. Here, we asked whether the activity of the sterol regulatory element-binding protein 2 (SREBP-2) pathway, which regulates the cholesterol content in membranes, affects the susceptibility of target cells toward toxins A and B...
December 28, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Ying Cai, Wen Liu, Yixuan Lin, Shaobao Zhang, Baorong Zou, Dan Xiao, Lei Lin, Yuping Zhong, Haihui Zheng, Qiongfeng Liao, Zhiyong Xie
BACKGROUND AND AIM: Inflammatory bowel disease (IBD) results from a dysregulated immune response to intestinal microbial flora in individuals with genetic predisposition(s). This study aimed to determine the effects of compound polysaccharides (CP) containing yam polysaccharide and inulin on the rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to explain the mechanism in terms of gut microbiota composition and function. METHODS: Male SD rats were divided into three groups: the control group, the model group and the CP group...
December 27, 2018: Journal of Gastroenterology and Hepatology
Alice Prigent, Jacques Gonzales, Tony Durand, Catherine Le Berre-Scoul, Malvyne Rolli-Derkinderen, Michel Neunlist, Pascal Derkinderen
The protein alpha-synuclein whose expression is strongly implicated in Parkinson's disease (PD) is not only expressed in the central nervous system (CNS) but also in the enteric nervous system (ENS). The growing body of evidence suggesting that gastrointestinal inflammation is involved in the development of PD led us to investigate the effects of inflammation on alpha-synuclein expression in primary culture of rat ENS and in mice with dextran sulfate sodium (DSS)-induced colitis. Using western blot and qPCR, we found that both LPS and a combination of TNF-α and IL1-β (TNF-α/IL1-β) decreased the expression levels of alpha-synuclein in primary culture of rat ENS, an effect that was prevented in the presence of the p38 inhibitors SB203580 and BIRB 796...
December 27, 2018: Journal of Neurochemistry
O B Ibitoye, T O Ajiboye
Menadione (Vitamin K3 ) is an over-the-counter (OTC) drug used in the treatment of abdominal cramps, colitis, diarrhea, hay fever, hemorrahage, hypoprothrombinemia, and joint pains. In this study, we evaluated the protective influence of protocatechuic acid on menadione-induced hepatotoxicity in rats. Rats were randomized into five groups (A-E) of five rats each. Control rats orally received 1% dimethyl sulfoxide (DMSO) in distilled water (the vehicle for protocatechuic administration) for 7 days. In addition, control rats intraperioneally received olive oil (vehicle for menadione administration) on the 7th day...
December 26, 2018: Drug and Chemical Toxicology
Aliakbar Yousefi-Ahmadipour, Amir Rashidian, Mohammad Reza Mirzaei, Alireza Farsinejad, Fatemeh PourMohammadi-Nejad, Mahmoud Ghazi-Khansari, Jafar Ai, Sadegh Shirian, Amir Allahverdi, Jamileh Saremi, Somayeh Ebrahimi-Barough
Adipose derived mesenchymal stem cells (ASCs) transplantation is a novel immunomodulatory therapeutic tool to ameliorate the symptom of inflammatory bowel disease (IBD). The objective of this study was to investigate the therapeutic effects of combined sufasalazine and ASCs therapy in a rat model of IBD. After induction of colitis in rats, ASCs were cultured and intraperitoneally injected (3 × 106 cells/kg) into the rats on Days 1 and 5 after inducing colitis, in conjunction with daily oral administration of low dose of sulfasalazine (30 mg/kg)...
December 23, 2018: Journal of Cellular Physiology
Souvik Mohanta, Sachin Kumar Singh, Bimlesh Kumar, Monica Gulati, Rajesh Kumar, Ankit Kumar Yadav, Sheetu Wadhwa, Jivan Jyoti, Sananda Som, Kamal Dua, Narendra Kumar Pandey
Modified Apple Polysaccharide (MAP) has been reported to cure colorectal diseases by up-regulating apoptosis and down regulating metastasis. In the present study, mesalamine (MES) and MAP mini tablets have been prepared and co-administered with probiotics to provide site specific release of drug. Probiotics along with MAP, which acts as a prebiotic would replenish the colonic microflora that have been compromised due to colorectal pathology. MES mini tablets were prepared keeping guar gum in the core and coating them with Eudragit S100 and guar gum...
December 17, 2018: International Journal of Biological Macromolecules
Carolina Pellegrini, Matteo Fornai, Rocchina Colucci, Laura Benvenuti, Vanessa D'Antongiovann, Gianfranco Natale, Federica Fulceri, Marta Giorgis, Elisabetta Marini, Simone Gastaldi, Massimo Bertinaria, Corrado Blandizzi, Luca Antonioli
Nucleotide-binding oligomerization domain leucine rich repeat and pyrin domain-containing protein 3 (NLRP3) inflammasome is pivotal in maintaining intestinal homeostasis and sustaining enteric immune responses in the setting of inflammatory bowel diseases. Drugs acting as NLRP3 blockers could represent innovative strategies for treatment of bowel inflammation. This study was performed in rats with dinitrobenzenesulfonic acid (DNBS)-induced colitis, to investigate how the direct blockade of NLRP3 inflammasome with an irreversible inhibitor (INF39) compares with Ac-YVAD-cmk (YVAD, caspase-1 inhibitor) and anakinra (IL-1β receptor antagonist), acting downstream on NLRP3 signaling...
2018: Frontiers in Pharmacology
Jipan Liu, Wei Sun, Xuerong Zhao, Bingmao Li, Chengjun Wang
Objective To investigate the effect of sanshensancao mixture on the histologic injury of colonic mucosa and the level of IL-12P70, TNF-α and IFN-γ in rats with ulcerative colitis(UC). Methods The rats were randomly divided into control group, model group and sanshensancao mixture group, with 10 rats in each group. The control group was fed with conventional diet, the sanshensancao mixture group and model group were established UC model by sodium trinitrobenzene sulfonate(TNBS)-ethanol induction method. Then 4...
October 2018: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Vijay L Kumar, Abhimanu Pandey, Sneh Verma, Prasenjit Das
Calotropis procera, a latex producing plant is known to possess medicinal properties including its beneficial effect in gastrointestinal disorders. The anti-inflammatory effect of its latex in various experimental models is noteworthy and in light of this the present study was carried out with an objective to evaluate its efficacy in ulcerative colitis, an inflammatory condition of the colon. Colitis was induced in rats by acetic acid and the rats were divided into four groups where one group served as experimental control and the other groups were treated with two doses of methanol extract of dried latex of C...
January 2019: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Ryo Kutsuna, Tohru Miyoshi-Akiyama, Koji Mori, Masahiro Hayashi, Junko Tomida, Yuji Morita, Kaori Tanaka, Yoshiaki Kawamura
Taxonomic studies of strain PAGU 1678T , an obligately anaerobic, Gram-stain positive, spore-forming bacterium isolated from Biobreeding rat feces, were performed. This strain has been demonstrated that the ability to exacerbate pathosis in a mouse model of dextran sulfate sodium-induced ulcerative colitis. Phylogenetic analysis based on the 16S rRNA gene showed high homology with Paraclostridium bifermentans. To clear the accurate taxonomic position of strain PAGU 1678T , a comparative taxonomic study using P...
December 14, 2018: Microbiology and Immunology
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