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https://read.qxmd.com/read/30769112/enhancing-parathyroid-gland-visualization-using-a-near-infrared-fluorescence-based-overlay-imaging-system
#1
Melanie A McWade, Giju Thomas, John Q Nguyen, Melinda E Sanders, Carmen C Solórzano, Anita Mahadevan-Jansen
BACKGROUND: Misidentifying parathyroid glands (PGs) during thyroidectomies or parathyroidectomies, could significantly increase post-operative morbidity. Imaging systems based on near-infrared autofluorescence (NIRAF) detection can localize PGs with high accuracy. These devices however depict NIRAF images on remote display monitors, where images lack spatial context and comparability with actual surgical field-of-view (FOV). In this study, we designed an Overlay Tissue Imaging System (OTIS) that detects tissue NIRAF and back-projects the collected signal as a visible image directly onto the surgical FOV instead of a display monitor, and tested its ability for enhancing parathyroid visualization...
February 12, 2019: Journal of the American College of Surgeons
https://read.qxmd.com/read/30768222/clinicopathologic-correlations-of-retinal-membranes-associated-with-intravitreal-stem-cell-injections
#2
Rehan M Hussain, Sander R Dubovy, Ajay E Kuriyan, Xiao Yi Zhou, Harry W Flynn, Thomas A Albini
The histologic findings of a subretinal band and epiretinal membrane (ERM) excised from two patients who developed retinal detachments (RDs) after non-U.S. Food and Drug Administration-regulated intravitreal "stem cell" injections are reported. Both membranes were composed of fibrocellular tissue that stained positively with Smooth Muscle Actin and Masson's trichrome, consistent with collagenous and smooth muscle composition. CD34 immunostain (for hematopoietic cells) was negative for the subretinal band and minimally positive for the ERM...
February 1, 2019: Ophthalmic Surgery, Lasers & Imaging Retina
https://read.qxmd.com/read/30768121/buccal-fat-pad-excision-hydrodissection-technique
#3
Luis C Valencia, Giovanny F Pérez, Jordan Kaplan, Ricardo Fernández-Riera
BACKGROUND: Buccal fat pad excision is a procedure in which the fat pad is extracted in order to achieve a more youthful appearance. OBJECTIVES: To describe an alternative technique that utilizes hydrodissection as a means to extract the buccal fat pad. METHODS: This is a controlled, prospective, randomized clinical study with two groups. Group A (n=27) underwent buccal fat pad excision with hydrodissection, and 15 ml of a vasoconstricting anesthetic solution was injected into the buccal fat pad...
February 15, 2019: Aesthetic Surgery Journal
https://read.qxmd.com/read/30767634/cryopreserved-amniotic-membrane-and-umbilical-cord-for-a-radiation-induced-wound-with-exposed-dura-a-case-report
#4
David Fernandez
An 87-year old male received surgical excision of scalp melanoma and subsequent radiotherapy due to metastasis to the skull. A radiation-induced wound developed with osteoradionecrosis that required necrotic bone excision resulting in a 7.5x8.5cm wound over the exposed dura, which remained non-healing despite many attempts by local wound care management. Due to the refractory nature of the wound, strips of cryopreserved umbilical cord (cUC) allograft were applied over the exposed dura resulting in significant vascular granulation tissue formation in the central wound bed within four weeks...
February 1, 2019: Journal of Wound Care
https://read.qxmd.com/read/30767440/integration-of-a-raman-spectroscopy-system-to-a-robotic-assisted-surgical-system-for-real-time-tissue-characterization-during-radical-prostatectomy-procedures
#5
Michael Pinto, Kevin C Zorn, Jean-Philippe Tremblay, Joannie Desroches, Frédérick Dallaire, Kelly Aubertin, Eric Marple, Chris Kent, Frederic Leblond, Dominique Trudel, Frederic Lesage
Surgical excision of the whole prostate through a radical prostatectomy procedure is part of the standard of care for prostate cancer. Positive surgical margins (cancer cells having spread into surrounding nonresected tissue) occur in as many as 1 in 5 cases and strongly correlate with disease recurrence and the requirement of adjuvant treatment. Margin assessment is currently only performed by pathologists hours to days following surgery and the integration of a real-time surgical readout would benefit current prostatectomy procedures...
February 2019: Journal of Biomedical Optics
https://read.qxmd.com/read/30767327/extended-surgical-safety-margins-and-ulceration-are-associated-with-an-improved-prognosis-in-pleomorphic-dermal-sarcomas-pds
#6
O D Persa, C Loquai, M Wobser, M Baltaci, S Dengler, A Kreuter, A Volz, M Laimer, M Emberger, M Doerler, C Mauch, D Helbig
BACKGROUND: Pleomorphic dermal sarcomas (PDS) are frequent UV-induced sarcomas of the skin of intermediate grade malignant potential. Despite the fact that PDS have a noteworthy potential to recur (up to 28%) as well as to metastasize (up to 20%), there are no specific clinical guidelines with respect to follow-up these patients. Moreover, little is known about clinical, histological or molecular prognostic factors in PDS. OBJECTIVE: The aim of the present study was to identify risk factors to predict relapse in a large multicenter sample cohort of PDS which could aid to optimize personalized treatment recommendations regarding surgical safety margins and adjuvant radiotherapy...
February 14, 2019: Journal of the European Academy of Dermatology and Venereology: JEADV
https://read.qxmd.com/read/30767257/lymphatic-supermicrosurgery-for-the-treatment-of-recurrent-lymphocele-and-severe-lymphorrhea
#7
Guido Giacalone, Takumi Yamamoto, Akitatsu Hayashi, Florence Belva, Mieke Gysen, Nobuko Hayashi, Nana Yamamoto, Isao Koshima
BACKGROUND: Lymphocele and lymphorrhea are frequent complications after lymph node excision. Recurrent lymphoceles and intractable lymphorrhea are particularly difficult to treat conservatively. We describe the outcomes of four patients with recurrent lymphocele and nine patients with persistent lymphorrhea that were treated by supermicrosurgery. METHODS: Four patients with recurrent lymphoceles with a size between 7 and 21 cm and located in the groin (n = 1) or upper leg (n = 3), were referred for surgical treatment between 2013 and 2017 after unsuccessful conservative therapy...
February 14, 2019: Microsurgery
https://read.qxmd.com/read/30766897/sufficient-therapeutic-effect-of-cryopreserved-frozen-adipose-derived-regenerative-cells-on-burn-wounds
#8
Yasuhiko Kaita, Takehiko Tarui, Hideaki Yoshino, Takeaki Matsuda, Yoshihiro Yamaguchi, Takatoshi Nakagawa, Michio Asahi, Masaaki Ii
Introduction: The purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs. Methods: Full thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses...
June 2019: Regenerative Therapy
https://read.qxmd.com/read/30766763/an-uncommon-pairing-of-common-tumors-case-report-of-ductal-carcinoma-in-situ-within-fibroadenoma
#9
Ashley Marumoto, Susan Steinemann, Nancy Furumoto, Stacey Woodruff
Fibroadenomas are common benign tumors of the female breast. In the appropriate clinical setting, they are often managed expectantly without excision. Rarely, cancer may arise within a fibroadenoma, and this diagnosis mandates prompt treatment for malignancy. We present the case of a 70-year-old Samoan woman with ductal carcinoma in situ (DCIS) arising within a fibroadenoma. Health care practitioners should be aware of the possibility, particularly in older women, of finding carcinoma within a fibroadenoma, which informs the rationale for prompt surgical evaluation and follow up of all breast masses...
February 2019: Hawai'i Journal of Medicine & Public Health: a Journal of Asia Pacific Medicine & Public Health
https://read.qxmd.com/read/30766738/novel-role-of-dehydrated-human-amnion-chorion-membrane-in-healing-of-denuded-ureter-in-robot-assisted-excision-of-endometriosis
#10
Osamah Hasan, Grant S Johnson, James J Siegert, Francisco Garcini, Thai T Nguyen
Ureteral injury is an uncommon but potentially morbid complication following any open or endoscopic pelvic procedure. Gynecologic surgeries alone make up 50 percent of nonurologic ureteral injuries leading to prolonged hospital stays, secondary interventions, and potential loss of renal function. The use of AmnioFix ® a processed dehydrated, immunologically privileged cellular amniotic membrane allograft has been well established in urologic and gynecologic procedures. These allografts contain human extracellular matrix components, growth factors, and cytokines that mediate inflammation and facilitate would healing...
2019: Case Reports in Obstetrics and Gynecology
https://read.qxmd.com/read/30765656/-a-case-report-of-breast-cancer-followed-up-as-intraductal-papilloma
#11
Mina Waraya, Keiko Hayashi, Madoka Inukai, Kouichi Yamamoto, Takeyoshi Habiro, Sayuri Oshida, Yoshimasa Kosaka, Norihiko Sengoku, Masahiko Watanabe
The patient was a 50-year-old woman. She had been diagnosed with bilateral breast tumors at another hospital 5 years previously and was followed up every 2 months. Ultrasonography showed hypoechoic masses in her breasts. The largest tumor in the right breast was 15mm in diameter and located in region A, while that in the left breast was 8mm in diameter and located in region B. Magnetic resonance imaging(MRI)showed multiple bilateral breast tumors. The largest tumor was 12mm in diameter and was suggestive of breast cancer...
January 2019: Gan to Kagaku Ryoho. Cancer & Chemotherapy
https://read.qxmd.com/read/30765448/spontaneous-cholecystocutaneous-fistula-empirically-treated-for-a-missed-diagnosis-managed-by-laparoscopy
#12
Manjunath Maruti Pol, Surabhi Vyas, Priyanka Singh, Yashwant Singh Rathore
A 70-year-old woman was referred to our hospital from primary health centre with complaints of pain in the abdomen, swelling and discharging sinus in the right hypochondrium since 2 years. She had received anti-tubercular treatment for 18 months as the wedge biopsy of the sinus tract suggested granulomatous lesion. As the symptoms did not subside she was referred to our hospital. Her blood investigation reports at our hospital were normal. Ultrasound of the abdomen suggested cholelithiasis with normal common bile duct...
February 13, 2019: BMJ Case Reports
https://read.qxmd.com/read/30764754/the-epigenetic-regulation-of-hsmar1-a-human-dna-transposon
#13
Sylvaine Renault, Murielle Genty, Alison Gabori, Catherine Boisneau, Charles Esnault, Thomas Dugé de Bernonville, Corinne Augé-Gouillou
BACKGROUND: Both classes of transposable elements (DNA and RNA) are tightly regulated at the transcriptional level leading to the inactivation of transposition via epigenetic mechanisms. Due to the high copies number of these elements, the hypothesis has emerged that their regulation can coordinate a regulatory network of genes. Herein, we investigated whether transposition regulation of HsMar1, a human DNA transposon, differs in presence or absence of endogenous HsMar1 copies. In the case where HsMar1 transposition is regulated, the number of repetitive DNA sequences issued by HsMar1 and distributed in the human genome makes HsMar1 a good candidate to regulate neighboring gene expression by epigenetic mechanisms...
February 14, 2019: BMC Genetics
https://read.qxmd.com/read/30764716/ovarian-reserve-reduction-with-surgery-is-not-correlated-with-the-amount-of-ovarian-tissue-inadvertently-excised-at-laparoscopic-surgery-for-endometriomas
#14
Ludovico Muzii, Chiara Di Tucci, Mara Di Feliciantonio, Giulia Galati, Irene Pecorella, Antonio Radicioni, Antonella Anzuini, Maria Grazia Piccioni, Felice Patacchiola, Pierluigi Benedetti Panici
The aim of the present study was to evaluate the effect of laparoscopic cystectomy on ovarian reserve by means of anti-Müllerian hormone (AMH) serial measurements and to compare AMH values with the number of inadvertently removed follicles in histological specimens. Fifty-two women were enrolled: 34 patients with endometriomas (group 1) and 18 patients with other benign ovarian cysts (group 2). All patients underwent laparoscopic cystectomy performed by a single experienced surgeon. The AMH was measured before, and 1, 3, and 6 months after cystectomy in group 1, and before and 6 months after surgery in group 2...
February 14, 2019: Reproductive Sciences
https://read.qxmd.com/read/30764712/perioperative-intervention-by-%C3%AE-blockade-and-nf-%C3%AE%C2%BAb-suppression-reduces-the-recurrence-risk-of-endometriosis-in-mice-due-to-incomplete-excision
#15
Qiqi Long, Hanxi Zheng, Xishi Liu, Sun-Wei Guo
Despite the demonstrated efficacy of surgical treatment of endometriosis, recurrence after surgery still remains a formidable challenge. Surgery, especially when performed repeatedly, decreases ovarian reserve. Clearly, control of recurrence is an unmet medical need. So far nearly all efforts to control recurrence have been devoted to the identification of risk factors, biomarkers, and postoperative medication. One area that has been completely overlooked is the possibility of perioperative intervention. In this study, we tested the hypothesis that perioperative use of a nonspecific β-blocker and/or a nuclear factor-κB (NF-κB) inhibitor can retard the growth of residual endometriotic lesions that are left intact in the primary surgery...
February 14, 2019: Reproductive Sciences
https://read.qxmd.com/read/30763888/deployment-of-dna-polymerases-beta-and-lambda-in-single-nucleotide-and-multinucleotide-pathways-of-mammalian-base-excision-dna-repair
#16
Upasna Thapar, Bruce Demple
There exist two major base excision DNA repair (BER) pathways, namely single-nucleotide or "short-patch" (SP-BER), and "long-patch" BER (LP-BER). Both pathways appear to be involved in the repair of small base lesions such as uracil, abasic sites and oxidized bases. In addition to DNA polymerase β (Polβ) as the main BER enzyme for repair synthesis, there is evidence for a minor role for DNA polymerase lambda (Polλ) in BER. In this study we explore the potential contribution of Polλ to both SP- and LP-BER in cell-free extracts...
February 4, 2019: DNA Repair
https://read.qxmd.com/read/30763374/public-finances-and-tobacco-taxation-with-product-variety-theory-and-application-to-senegal-and-nigeria
#17
Théophile T Azomahou, Racky Baldé, Abdoulaye Diagne, Pape Yona Mané, Ibrahima Sory Kaba
This study endeavors to answer two questions: which category of excise taxes is more appropriate for Senegal and Nigeria and which consequences an increase of the tobacco taxes would have on the price, the demand and the tax revenues in each one of the two countries? To answer these questions, we adopt a double approach: first, a theoretical model of taxation with variety; and second, a simulation model to answer the second question. The results of the theoretical model indicate that, in the context of excise taxation, the number of products variety-or that of cigarette brands-directly affects both the degree of market concentration and the marginal effects of specific and ad valorem excise taxes on the price of tobacco...
2019: PloS One
https://read.qxmd.com/read/30762924/spironolactone-induced-xpb-degradation-depends-on-cdk7-kinase-and-scf-fbxl-18-e3-ligase
#18
Masanobu Ueda, Kenkyo Matsuura, Hidehiko Kawai, Mitsuo Wakasugi, Tsukasa Matsunaga
The multi-subunit complex transcription factor IIH (TFIIH) has dual functions in transcriptional initiation and nucleotide excision repair (NER). TFIIH is comprised of two subcomplexes, the core subcomplex (7 subunits) including XPB and XPD helicases and the cyclin-dependent kinase (CDK)-activating kinase (CAK) subcomplex (3 subunits) containing CDK7 kinase. Recently, it has been reported that spironolactone, an anti-aldosterone drug, inhibits cellular NER by inducing proteasomal degradation of XPB and potentiates the cytotoxicity of platinum-based drugs in cancer cells, suggesting possible drug repositioning...
February 14, 2019: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://read.qxmd.com/read/30762545/svd-based-separation-of-stable-and-inertial-cavitation-signals-applied-to-passive-cavitation-mapping-during-hifu
#19
Parag V Chitnis, Caleb H Farny, Ronald A Roy
Detection of inertial and stable cavitation is important for guiding high-intensity focused ultrasound (HIFU). Acoustic transducers can passively detect broadband noise from inertial cavitation and the scattering of HIFU harmonics from stable cavitation bubbles. Conventional approaches to cavitation noise diagnostics typically involve computing the Fourier transform of the time domain noise signal, applying a custom comb-filter to isolate the frequency components of interest, followed by an Inverse-Fourier transform...
February 13, 2019: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
https://read.qxmd.com/read/30762430/sleeve-shaped-neurothekeoma-of-the-ulnar-nerve-a-unique-case-of-a-still-unclear-pathological-entity
#20
Ignazio G Vetrano, Vincenzo Levi, Bianca Pollo, Luisa Chiapparini, Giuseppe Messina, Vittoria Nazzi
BACKGROUND: Neurothekeomas are slow-growing, well-circumscribed benign neoplasms. They usually involve cutaneous or subcutaneous tissues. Although originally described as myxomas deriving from nerve sheath cells, their exact histological classification is still uncertain. Peripheral nerve localization is rarely reported. Here, we describe a unique case of sleeve-shaped neurothekeoma of the ulnar nerve, which was incidentally discovered during a cubital tunnel release surgery. METHODS: A 57-year-old man was admitted at our institution with clinical, ultrasonographic, and electromyographic findings highly suggestive of cubital tunnel syndrome...
February 14, 2019: Hand: Official Journal of the American Association for Hand Surgery
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