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metabolomics AND obesity

Anna Halama, Myint Myint Aye, Soha R Dargham, Michal Kulinski, Karsten Suhre, Stephen L Atkin
Background: Plasma elevated levels of branched chain amino acids (BCAA) and aromatic amino acids (AAA) have been associated with obesity and insulin resistance, but their relationship to stimulated insulin resistance (IR) in PCOS and in response to exercise is unknown. Indeed, it is unknown whether the mechanism of IR in PCOS is mediated through changes in the metabolome. Methods: Twelve women with polycystic ovary syndrome (PCOS) and ten age and body mass index matched controls completed an 8 week supervised exercise program at 60% maximal oxygen consumption...
2019: Frontiers in Endocrinology
Jose F Garcia-Mazcorro, Romina Pedreschi, Jialing Yuan, Jorge R Kawas, Boon Chew, Scot E Dowd, Giuliana Noratto
Apples contain bioactive compounds with the potential to alleviate clinical signs associated with obesity, a phenomenon likely related to the composition and function of the gut microbiota. The aim of this study was to investigate the effect of apple supplementation on the fecal microbiota and gut metabolites of Dawley Sprague rats fed a high-fat (HF group) or a low-fat (LF group) diet. The fecal microbiota was examined using 16S marker sequencing targeting the V4 region in a MiSeq instrument (Illumina). With the exception of Blautia, which was higher in supplemented rats compared to controls within the LF group, significant differences in fecal microbiota between supplemented rats and controls were only found in the HF group...
2019: PloS One
Carla Luis, Fernanda Duarte, Isabel Faria, Ivana Jarak, Pedro F Oliveira, Marco Alves, Raquel Soares, Rúben Fernandes
AIMS: Obesity is a complex health disorder and a trigger to many diseases like Diabetes mellitus (DM) and breast cancer (BrCa), both leading causes of morbidity and mortality worldwide. Also evidence demonstrates that abnormal glucose metabolism termed 'the Warburg effect' in cancer cell is closely associated with malignant phenotypes and promote the aggressiveness of several types of cancer, including BrCa. In this study, we evaluated the breast cancer cell metabolism in normoglycemia, hyperglycemia and in an obesity condition in order to clarify the potential underlined mechanisms that link these disorders...
March 9, 2019: Life Sciences
Magali Palau-Rodriguez, Mar Garcia-Aloy, Antonio Miñarro, M Rosa Bernal-Lopez, Carl Brunius, Ricardo Gómez-Huelgas, Rikard Landberg, Francisco J Tinahones, Cristina Andres-Lacueva
BACKGROUND & AIMS: The benefits of weight loss in subjects with metabolically healthy obesity (MHO) are still a matter of controversy. We aimed to identify metabolic fingerprints and their associated pathways that discriminate women with MHO with high or low weight loss response after a lifestyle intervention, based on a hypocaloric Mediterranean diet (MedDiet) and physical activity. METHODS: A UPLC-Q-Exactive-MS/MS metabolomics workflow was applied to plasma samples from 27 women with MHO before and after 12 months of a hypocaloric weight loss intervention with a MedDiet and increased physical activity...
February 16, 2019: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
Eun-Ju Kim, Young-Shick Hong, Seung-Ho Seo, Seong-Eun Park, Chang-Su Na, Hong-Seok Son
Sasang constitutional medicine classifies human beings into four types based on their physical and psychological characteristics. Despite its potential value in achieving personalized medicine, the diagnosis of sasang constitution (SC) type is complex and subjective. In this study, gas chromatography-mass spectrometry and 1 H nuclear magnetic resonance-based metabolic analyses were conducted to find maker metabolites in serum and urine according to different SC types. Although some samples were overlapped on orthogonal projection to latent structure discriminant analysis score plots, serum samples showed separation between different SC types...
2019: Evidence-based Complementary and Alternative Medicine: ECAM
Tanya L Alderete, Ran Jin, Douglas I Walker, Damaskini Valvi, Zhanghua Chen, Dean P Jones, Cheng Peng, Frank D Gilliland, Kiros Berhane, David V Conti, Michael I Goran, Lida Chatzi
OBJECTIVE: To examine the prospective associations between exposure to perfluoroalkyl substances (PFASs) and longitudinal measurements of glucose metabolism in high-risk overweight and obese Hispanic children. METHODS: Forty overweight and obese Hispanic children (8-14 years) from urban Los Angeles underwent clinical measures and 2-hour oral glucose tolerance tests (OGTT) at baseline and a follow-up visit (range: 1-3 years after enrollment). Baseline plasma perfluorooctanoic acid (PFOA), perfluorooctane sulfonate (PFOS), perfluorohexane sulfonic acid (PFHxS), and the plasma metabolome were measured by liquid-chromatography with high-resolution mass spectrometry...
March 4, 2019: Environment International
Soravis Osataphan, Chiara Macchi, Garima Singhal, Jeremy Chimene-Weiss, Vicencia Sales, Chisayo Kozuka, Jonathan M Dreyfuss, Hui Pan, Yanin Tangcharoenpaisan, Jordan Morningstar, Robert Gerszten, Mary-Elizabeth Patti
Pharmacologic inhibition of the renal sodium/glucose cotransporter-2 induces glycosuria and reduces glycemia. Given that SGLT2 inhibitors (SGLT2i) reduce mortality and cardiovascular risk in type 2 diabetes, improved understanding of molecular mechanisms mediating these metabolic effects is required. Treatment of obese but nondiabetic mice with the SGLT2i canagliflozin (CANA) reduces adiposity, improves glucose tolerance despite reduced plasma insulin, increases plasma ketones, and improves plasma lipid profiles...
March 7, 2019: JCI Insight
Francois Brial, Aurélie Le Lay, Lyamine Hedjazi, Tsz Tsang, Jane F Fearnside, Georg W Otto, Fawaz Alzaid, Steven P Wilder, Nicolas Venteclef, Jean-Baptiste Cazier, Jeremy K Nicholson, Chris Day, Alastair D Burt, Ivo G Gut, Mark Lathrop, Marc-Emmanuel Dumas, Dominique Gauguier
Non-alcoholic fatty liver disease (NAFLD) is often associated with obesity and type 2 diabetes. To disentangle etiological relationships between these conditions and identify genetically-determined metabolites involved in NAFLD processes, we mapped 1 H nuclear magnetic resonance (NMR) metabolomic and disease-related phenotypes in a mouse F2 cross derived from strains showing resistance (BALB/c) and increased susceptibility (129S6) to these diseases. Quantitative trait locus (QTL) analysis based on single nucleotide polymorphism (SNP) genotypes identified diet responsive QTLs in F2 mice fed control or high fat diet (HFD)...
March 6, 2019: Scientific Reports
Diana Valesca Carvalho, Lorena Mara Alexandre Silva, Elenilson Godoy Alves Filho, Flávia Almeida Santos, Renan Pereira de Lima, Ana Flávia Seraine Custódio Viana, Paulo Iury Gomes Nunes, Said Gonçalves da Cruz Fonseca, Tiago Sousa de Melo, Daniel de Araújo Viana, Maria Izabel Gallão, Edy Sousa de Brito
Dietary fiber intake plays an important role in the prevention of obesity. This study aimed at investigating the effect of cashew fiber without low molecular weight compounds (CABwc) on obesity prevention and metabolomics in a murine model of diet-induced obesity. Mice were fed a chow diet (CD), a high-fat diet (HFD) or a high-fat diet supplemented with CABwc (10%) (HFD-CABwc) for 15 weeks. The body weight, abdominal fat, serum glucose levels, insulin and lipid profiles, satiety hormones such as leptin and ghrelin, digestive enzymes such as amylase and lipase, and inflammatory mediators such as TNF-α, IL-6, and adiponectin were measured, in addition to performing serum and hepatic tissue analyses...
March 6, 2019: Food & Function
Martina Cermakova, Helena Pelantová, Barbora Neprasova, Blanka Sediva, Lenka Maletinska, Jaroslav Kunes, Petra Tomasova, Blanka Zelezna, Marek Kuzma
In this study, the combination of metabolomics and standard biochemical and biometric parameters was used to describe the metabolic effects of diet-induced obesity and its treatment with the novel anti-obesity compound palm11 -PrRP31 (palmitoylated prolactin-releasing peptide) in spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto rats (WKY). The results showed that SHR on a high-fat (HF) diet were normoglycemic with obesity and hypertension, while WKY on the HF diet were normotensive and obese with prediabetes...
February 27, 2019: Journal of Proteome Research
Yifeng Zeng, Yixuan Lin, Lin Li, Yuan Li, Xiaojun Zhang, Mengxia Wang, Yongxiong Chen, Liang Luo, Biyu Lu, Zhiyong Xie, Qiongfeng Liao
Gut microbiota-host co-metabolites play an essential role in maintaining homeostasis, and their concentration changes are closely related to a variety of diseases. Developing a targeted metabolomics analytical platform for these co-metabolites will help to elucidate the relationship between intestinal flora and host. Here we present a simple and sensitive liquid chromatography-tandem mass spectrometry method for the analysis of nine gut microbiota-host co-metabolites in rat serum, urine and feces. The compounds were separated on a reversed-phase C18 column using gradient elution with a solvent system consisting of methanol and water (containing 0...
March 15, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Saradha Baskaran, Ashok Agarwal, Kristian Leisegang, Peter Natesan Pushparaj, Manesh Kumar Panner Selvam, Ralf Henkel
PURPOSE: Male infertility is emerging as a major, escalating global health problem that imposes the need to investigate research trends in male infertility. The purpose of this study is to analyze male infertility research trends in the past 20 years using the bibliometric database from Scopus. MATERIALS AND METHODS: In order to perform an in-depth bibliometric analysis, we propose a 'Funnel Model', which includes several layers representing different sub-areas of male infertility research...
February 20, 2019: World Journal of Men's Health
Yu Gu, Can Liu, Ningning Zheng, Wei Jia, Weidong Zhang, Houkai Li
Obesity is characterized with high heterogeneity due to genetic abnormality, energy imbalance and/or gut dysbiosis. Obesity-prone (OP) and -resistant (OR) phenotypes are frequently observed in rodents even under high-fat diet (HFD). However, the underlying mechanisms are largely unknown. Male C57BL/6J mice were fed with chow or HFD for 8 weeks. OP and OR mice were defined based on body weight gain, and an integrated serum metabolic and gut microbial profiling was performed by gas chromatography-mass spectroscopy-based metabolomic and pyrosequencing of 16S rDNA of cecum contents...
February 22, 2019: Journal of Proteome Research
Xinxin Ke, Alesia Walker, Sven-Bastiaan Haange, Ilias Lagkouvardos, Yuwen Liu, Philippe Schmitt-Kopplin, Martin von Bergen, Nico Jehmlich, Xin He, Thomas Clavel, Peter C K Cheung
OBJECTIVE: The gut microbiota is an important influencing factor of metabolic health. Although dietary interventions with probiotics, prebiotics, and synbiotics can be effective means to regulate obesity and associated comorbidities, the underlying shifts in gut microbial communities, especially at the functional level, have not been characterized in great details. In this study, we sought to investigate the effects of synbiotics on the regulation of gut microbiota and the alleviation of high-fat diet (HFD)-induced metabolic disorders in mice...
February 5, 2019: Molecular Metabolism
M Federici
INTRODUCTION: The gut microbiome is emerging as an important player in the field of metabolic disorders. MATERIALS AND METHODS: Currently, several studies are ongoing to determine whether the effect of gut microbiome on obesity, type 2 diabetes, non-alcoholic fatty liver disease, and other metabolic diseases is determined by singular species or rather by a functional role of bacterial metabolism at higher taxonomical level. Deciphering if a single or more species are responsible for metabolic traits or rather microbial metabolic pathways are responsible for effects on host metabolism may help to identify appropriate dietary interventions to support microbial functions according to the prevalent host disease...
February 20, 2019: Journal of Endocrinological Investigation
Karl Walter Bock
Diverse physiologic functions of AHR, a transcription factor discovered in studies of dioxin toxicity, are currently elucidated in many laboratories including chemical and microbial defense, immunity and myelopoiesis. Accumulating evidence suggests that AHR may also be involved in obesity and TCDD-mediated lethality in sensitive species. Underlying mechanisms include NAD+ - and sirtuin-mediated deregulation of lipid, glucose and NAD+ homeostasis. Progress in NAD metabolome research suggests large consumption of NAD+ by NAD glycohydrolases (NADases) and NAD-dependent sirtuins...
February 16, 2019: Biochemical Pharmacology
Michael W O'Reilly, Connar Sj Westgate, Catherine Hornby, Hannah Botfield, Angela E Taylor, Keira Markey, James L Mitchell, William J Scotton, Susan P Mollan, Andreas Yiangou, Carl Jenkinson, Lorna C Gilligan, Mark Sherlock, James Gibney, Jeremy W Tomlinson, Gareth G Lavery, David J Hodson, Wiebke Arlt, Alexandra J Sinclair
Idiopathic intracranial hypertension (IIH) is a condition of unknown etiology, characterized by elevated intracranial pressure frequently manifesting with chronic headaches and visual loss. Similar to polycystic ovary syndrome (PCOS), IIH predominantly affects obese women of reproductive age. In this study, we comprehensively examined the systemic and cerebrospinal fluid (CSF) androgen metabolome in women with IIH in comparison to sex-, body mass index- and age-matched control groups with either simple obesity and PCOS, i...
February 12, 2019: JCI Insight
Melanie Cree-Green, Anne-Marie Carreau, Haseeb Rahat, Yesenia Garcia-Reyes, Bryan C Bergman, Laura Pyle, Kristen J Nadeau
Polycystic Ovarian Syndrome (PCOS) is associated with insulin resistance (IR) and altered muscle mitochondrial oxidative phosphorylation. IR in adults with obesity and diabetes is associated with changes in amino acid, free fatty acid (FFA) and mitochondrial acylcarnitine (AC) metabolism. We sought to determine if these metabolites are associated with IR and/or androgens in youth-onset PCOS. We enrolled obese girls with PCOS [n = 15, 14.5 ± 1.6 years, BMI %ile 98.5 ± 1.0] and without PCOS [n = 6, 13.2 ± 1...
February 12, 2019: American Journal of Physiology. Endocrinology and Metabolism
I Alexandru Bobulescu, Sun K Park, L H Richie Xu, Francisco Blanco, John Poindexter, Beverley Adams-Huet, Taylor L Davidson, Khashayar Sakhaee, Naim M Maalouf, Orson W Moe
BACKGROUND AND OBJECTIVES: Idiopathic uric acid nephrolithiasis, which is closely associated with obesity and the metabolic syndrome, is increasing in prevalence. Unduly acidic urine pH, the quintessential pathophysiologic feature of this disease, is in part explained by inadequate excretion of the principal urinary buffer ammonium. The role of net acid excretion in the pathogenesis of uric acid nephrolithiasis is incompletely understood. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: We compared acid-base parameters of patients with idiopathic uric acid nephrolithiasis with matched control subjects under controlled diets in an inpatient metabolic unit...
February 11, 2019: Clinical Journal of the American Society of Nephrology: CJASN
Chunxue Yang, Chi-Ming Wong, Juntong Wei, Arthur C K Chung, Zongwei Cai
Adipocyte differentiation is closely associated with obesity and obesity-induced metabolic disorders. Epidemiological studies have demonstrated the association of obesity with environmental pollutants, such as polybrominated diphenyl ethers (PBDEs), common flame retardants in various consumer products. However, their obesogenic effects and mechanism are underexplored. We employed non-targeted metabolomics studies based on liquid chromatography-high resolution mass spectrometry to determine how 2,2',4,4'-tetra-brominated biphenyl ether (BDE 47), one of the main congeners of PBDEs detected in human tissue, promotes adipocyte differentiation of mouse preadipocyte 3 T3-L1 cells...
December 10, 2018: Science of the Total Environment
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