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https://read.qxmd.com/read/30772377/ubc9-regulates-cardiac-sodium-channel-na-v-1-5-ubiquitination-degradation-and-sodium-current-density
#1
Bo Tang, Yushuang Hu, Zhijie Wang, Chen Cheng, Pengyun Wang, Lina Liang, Hongbo Xiong, Chunyan Luo, Chengqi Xu, Qiuyun Chen, Qing Kenneth Wang
Voltage-gated sodium channel Nav 1.5 is critical for generation and conduction of cardiac action potentials. Mutations and expression level changes of Nav 1.5 are associated with cardiac arrhythmias and sudden death. The ubiquitin (Ub) conjugation machinery utilizes three enzyme activities, E1, E2, and E3, to regulate protein degradation. Previous studies from us and others showed that Nedd4-2 acts as an E3 ubiquitin-protein ligase involved in ubiquitination and degradation of Nav 1.5, however, more key regulators remain to be identified...
February 14, 2019: Journal of Molecular and Cellular Cardiology
https://read.qxmd.com/read/30771493/viral-genome-integration-of-canine-papillomavirus-16
#2
Jennifer Luff, Michelle Mader, Peter Rowland, Monica Britton, Joseph Fass, Hang Yuan
Papillomaviruses infect humans and animals, most often causing benign proliferations on skin or mucosal surfaces. Rarely, these infections persist and progress to cancer. In humans, this transformation most often occurs with high-risk papillomaviruses, where viral integration is a critical event in carcinogenesis. The first aim of this study was to sequence the viral genome of canine papillomavirus (CPV) 16 from a pigmented viral plaque that progressed to metastatic squamous cell carcinoma in a dog. The second aim was to characterize multiple viral genomic deletions and translocations as well as host integration sites...
February 13, 2019: Papillomavirus Research
https://read.qxmd.com/read/30770471/cholesterol-depletion-inhibits-na-k-atpase-activity-in-a-near-native-membrane-environment
#3
Alvaro Garcia, Bogdan Lev, Khondker R Hossain, Amy Gorman, Dil Diaz, Thi Hanh Nguyen Pham, Flemming Cornelius, Toby W Allen, Ronald J Clarke
Cholesterol's effects on Na+ ,K+ -ATPase reconstituted in phospholipid vesicles have been extensively studied. However, previous studies have reported both cholesterol-mediated stimulation and inhibition of Na+ ,K+ -ATPase activity. Here, using partial reaction kinetics determined via stopped-flow experiments, we studied cholesterol's effect on Na+ ,K+ -ATPase in a near-native environment in which purified membrane fragments were depleted of cholesterol with methyl-β-cyclodextrin (mβCD). The mβCD-treated Na+ ,K+ -ATPase had significantly reduced overall activity and exhibited decreased observed rate constants for ATP phosphorylation (ENa+ 3 → E2P, i...
February 15, 2019: Journal of Biological Chemistry
https://read.qxmd.com/read/30769050/the-protective-effects-of-the-antioxidant-n-acetylcysteine-nac-against-oxidative-stress-associated-apoptosis-evoked-by-the-organophosphorus-insecticide-malathion-in-normal-human-astrocytes
#4
Pochuen Sheih, Chung-Ren Jan, Wei-Zhe Liang
Malathion is one of the most widely used organophosphorus insecticides in agriculture. However, malathion may be involved in the etiology of human brain dysfunction. Induction of ROS has been proposed as a mechanism of malathion-induced poisoning cases, but there are few data regarding the effects of malathion on oxidative stress-associated neurotoxicity in human glial cells. The aim was to explore the mechanism underlying effects of malathion on neurotoxicity in Gibco® Human Astrocytes (GHA cells) and evaluate the protective effects of the antioxidant (N-acetylcysteine, NAC)...
February 12, 2019: Toxicology
https://read.qxmd.com/read/30769006/a-central-hydrophobic-e1-region-controls-the-ph-range-of-hepatitis-c-virus-membrane-fusion-and-susceptibility-to-fusion-inhibitors
#5
Dominic H Banda, Paula M Perin, Richard J P Brown, Daniel Todt, Wladimir Solodenko, Patrick Hoffmeyer, Kamlesh Kumar Sahu, Michael Houghton, Philip Meuleman, Rolf Müller, Andreas Kirschning, Thomas Pietschmann
BACKGROUND & AIMS: Hepatitis C virus infection causes chronic liver disease. Antivirals have been developed and cure infection. However, resistance can emerge and salvage therapies with alternative modes of action could be useful. Several licensed drugs have emerged as HCV entry inhibitors representing candidates for drug repurposing. We aimed to dissect their mode of action, identify improved derivatives and determine their viral targets. METHODS: HCV entry inhibition was tested for a panel of structurally related compounds, using chimeric viruses representing diverse genotypes, in addition to viruses containing previously determined resistance mutations...
February 12, 2019: Journal of Hepatology
https://read.qxmd.com/read/30768223/clinical-features-and-multi-modality-imaging-of-isolated-retinal-astrocytic-hamartoma
#6
Andrew W Stacey, Maria Pefkianaki, Tomas Ilginis, Michael Michaelides, Phill Hykin, Andrew Webster, Anthony T Moore, Mandeep S Sagoo
BACKGROUND AND OBJECTIVE: To identify the clinical and imaging characteristics of isolated retinal astrocytic hamartomas (IRAH). PATIENTS AND METHODS: A case series of eight patients diagnosed with IRAH. RESULTS: The average age at diagnosis was 32 years (range: 9 years to 80 years). After a median follow-up time of 59 months, none of the lesions had demonstrated any change or growth. Fundus fluorescein angiogram identified hyperfluorescence in five of six imaged lesions...
February 1, 2019: Ophthalmic Surgery, Lasers & Imaging Retina
https://read.qxmd.com/read/30764934/study-on-the-in-vitro-and-in-vivo-antibacterial-activity-and-biocompatibility-of-novel-tin-ag-multilayers-immobilized-onto-biomedical-titanium
#7
Ming Ma, Rongxin Wan, Huanhuan Gong, Xiaofei Lv, Shanshan Chu, Dejun Li, Hanqing Gu, Cheng Peng
To determine the short- and long-term antibacterial properties of a novel biomedical titanium alloy to ensure excellent biocompatibility of the TiN/Ag multilayers loaded with different doses of Ag+ . First, nanosized TiN/Ag multilayers were accumulated onto titanium alloy (Ti-6Al-4V) substrates via multi-arc ion plating. Then, the multilayers were implanted with different doses of silver ions (1×1017 ions/cm², 1×1018 ions/cm², 5×1016 ions/cm², and 5×1017 ions/cm²). Both short- and long-term antibacterial properties against Streptococcus mutans and Staphylococcus aureus were assessed via unique methods...
July 1, 2019: Journal of Nanoscience and Nanotechnology
https://read.qxmd.com/read/30763810/multi-phase-distribution-and-risk-assessment-of-endocrine-disrupting-chemicals-in-the-surface-water-of-the-shaying-river-huai-river-basin-china
#8
Yu Huang, Xianchuan Xie, Li Jun Zhou, Xiaowen Ji, Bin Gao, Gui Zhou Xu, Aimin Li
Herein we investigated the multi-phase distribution and estrogenic effects of endocrine disrupting chemicals (EDCs) in suspended particulate matter (SPM), colloids, and soluble phases from the Shaying River to assess the composition of estrogenic compounds and associated estrogenic risk. The yeast two hybrid (YES) method, cross-flow ultrafiltration (CFUF), and LC-MS/MS were employed. Risk quotient (RQ) values ranged from 0.72 to 3.88, revealing that the Shaying River posed high estrogenic risk to aquatic organisms...
February 11, 2019: Ecotoxicology and Environmental Safety
https://read.qxmd.com/read/30763774/g1p-8-rotavirus-in-children-with-severe-diarrhea-in-the-post-vaccine-introduction-era-in-brazil-evidence-of-reassortments-and-structural-modifications-of-the-antigenic-vp7-and-vp4-regions
#9
F S Santos, E C Sousa Junior, S F S Guerra, P S Lobo, E T Penha Junior, A B F Lima, C B G Vinente, E H N Chagas, M C A Justino, A C Linhares, J Matthijnssens, L S Soares, J D P Mascarenhas
Worldwide rotaviruses A (RVA) are responsible for approximately 215,000 deaths annually among children aged <5 years. RVA G1P[8] remains associated with >50% of gastroenteritis cases in this age group. The aim of this study was to assess the genetic variability of G1P[8] strains detected in children with severe diarrhea in Belém, Pará, Brazil, during the post-rotavirus vaccine introduction era. Phylogenetic analysis clustered the VP4 and VP7 genes of 40 samples selected between 2009 and 2011 into lineages found to be different from the Rotarix® vaccine strain...
February 11, 2019: Infection, Genetics and Evolution
https://read.qxmd.com/read/30763773/whole-genome-characterisation-of-g11p-25-and-g9p-19-rotavirus-a-strains-from-adult-patients-with-diarrhoea-in-nepal
#10
Hanae Takatsuki, Chantal Ama Agbemabiese, Toyoko Nakagomi, Sher Bahadur Pun, Punita Gauchan, Hideki Muto, Hiroshi Masumoto, Ryuichiro Atarashi, Osamu Nakagomi, Basu Dev Pandey
Rotavirus A (RVA) causes acute diarrhoea in children and less frequently in adults. However, the knowledge about the genotype distribution of RVA strains circulating in adults is limited particularly in developing countries. This study aimed to characterise the RVA strains detected from adult patients with diarrhoea in Nepal. A total of 47 RVA positive stool samples from adult patients with diarrhoea in Kathmandu, Nepal during 2007-2008 were examined for the G and P genotypes by sequencing. Nearly half (49%) of the samples were genotyped as G9P[8] (n = 23), G1P[8], G2P[4] (n = 5 each), G12P[8] (n = 4), G12P[6] (n = 3), G1P[6] (n = 2), G3P[8] and G9P[6] (n = 1 each)...
February 11, 2019: Infection, Genetics and Evolution
https://read.qxmd.com/read/30761410/the-novel-x-ray-visible-zein-based-non-adhesive-precipitating-liquid-embolic-hei-e1_2017-an-exploratory-study
#11
Dominik F Vollherbst, Thuy D Do, Manfred Jugold, Viktoria Eichwald, Stephan Macher-Göppinger, Philippe L Pereira, Martin Bendszus, Markus A Möhlenbruch, Götz M Richter, Hans U Kauczor, Christof M Sommer
PURPOSE: To investigate the novel zein-based non-adhesive precipitating liquid embolic HEIE1_2017 . MATERIALS AND METHODS: Zein-based liquid embolics are an own class of embolization material. In this study, HEIE1_2017 , a novel zein-based liquid embolic, was investigated. Visibility was assessed in vitro in CT and MRI phantoms, embolization characteristics were assessed in vivo in the kidneys of 12 pigs. Components of HEIE1_2017 were zein as occlusion material, ethanol as solvent, and iodized oil as radiopaque material...
February 13, 2019: Cardiovascular and Interventional Radiology
https://read.qxmd.com/read/30760153/is-antithrombotic-therapy-necessary-following-replantation-of-an-amputated-digit
#12
Takeo Matsusue
BACKGROUND: Antithrombotic therapy following replantation remains controversial, and the survival of replanted digits is affected by various other factors, such as the state of vascular damage and the surgeon's level of skill. The present study's aim is to obtain clinical evidence for postoperative antithrombotic therapy in replantation, with antithrombotic therapy being the only variable. METHODS: This was a single-center retrospective study of patients who underwent replantation of a completely amputated digit by the same surgeon...
March 2019: Journal of Hand Surgery Asian-Pacific Volume
https://read.qxmd.com/read/30759249/a-novel-interplay-between-irisin-and-pth-from-basic-studies-to-clinical-evidence-in-hyperparathyroidism
#13
Andrea Palermo, Lorenzo Sanesi, Graziana Colaianni, Gaia Tabacco, Anda Mihaela Naciu, Roberto Cesareo, Claudio Pedone, Diana Lelli, Giacomina Brunetti, Giorgio Mori, Silvia Colucci, Silvia Manfrini, Nicola Napoli, Maria Grano
Context: Irisin is a hormone like-molecule that is cleaved and secreted by an unknown protease from fibronectin type III domain-containing protein 5 (FNDC5) and it ameliorates bone status, muscle atrophy and influences energy homeostasis. PTH exerts several metabolic effects that may interact with irisin's ones. Objectives: To test the hypothesis that irisin and PTH mutually affect their biological action, we evaluated the FNDC5 mRNA and protein expression in myotubes treated with PTH (1-34) and PTH-r mRNA expression in osteoblasts treated with rec-irisin...
February 13, 2019: Journal of Clinical Endocrinology and Metabolism
https://read.qxmd.com/read/30759109/description-of-a-new-species-of-the-neotropical-cichlid-genus-gymnogeophagus-miranda-ribeiro-1918-teleostei-cichliformes-from-the-middle-paran%C3%A3-basin-misiones-argentina
#14
Felipe Alonso, Guillermo E Terán, Gastón Aguilera, Oldřich Říčan, Jorge Casciotta, Wilson Sebastián Serra, Adriana Almirón, Mauricio F Benítez, Ignacio García, Juan Marcos Mirande
Gymnogeophagus jaryi, new species, is described from Southern tributaries of the Middle Paraná basin in Misiones. It can be distinguished from all other members of the genus, except from G. australis and G. caaguazuensis, by the presence of a hyaline to grey anterior portion of the dorsal fin. Gymnogeophagus jaryi differs from G. caaguazuensis by a longer caudal peduncle, caudal fin not lyrate, central portion of scales on dorsal portion of trunk light iridescent blue and by white spots in soft portion of dorsal fin in adult males, and from G...
2019: PloS One
https://read.qxmd.com/read/30754651/zoledronic-acid-modulation-of-trpv1-channel-currents-in-osteoblast-cell-line-and-native-rat-and-mouse-bone-marrow-derived-osteoblasts-cell-proliferation-and-mineralization-effect
#15
Rosa Scala, Fatima Maqoud, Mariacristina Angelelli, Ramon Latorre, Maria Grazia Perrone, Antonio Scilimati, Domenico Tricarico
Bisphosphonates (BPs) reduce bone pain and fractures by balancing the osteoblast/osteoclast ratio. The behavior of ion channels in the presence of BPs is not known. To investigate this, the effect of zoledronic acid BP (ZOL) (3 × 10-8 to 5 × 10-4 M) treatment, on ion channels, cell proliferation, and mineralization, has been investigated on preosteoclast-like cells, RAW264.7, preosteoblast-like cells MC3T3-E1, and rat/mouse native bone marrow-derived osteoblasts. In whole-cell patch clamp on cell line- and bone marrow-derived osteoblasts, ZOL potentiated outward currents...
February 11, 2019: Cancers
https://read.qxmd.com/read/30747974/pre-aponeurotic-fat-prolapse-following-levator-resection-for-congenital-ptosis
#16
Andrea A Tooley, Elizabeth A Bradley, Brian G Mohney
The authors describe three children who developed excess overhanging upper eyelid tissue following unilateral levator resection for congenital ptosis. This was thought to be pre-aponeurotic fat herniation. A second procedure, a variation of sulcoplasty, was performed at a mean 4.3 months later and all three children showed improved sulcus and crease symmetry. [J Pediatr Ophthalmol Strabismus. 2019;56:e1-e4.].
February 8, 2019: Journal of Pediatric Ophthalmology and Strabismus
https://read.qxmd.com/read/30745919/the-effect-of-office-bleaching-on-the-color-and-bond-strength-of-resin-restorations
#17
Homayoon Alaghehmand, Marzieh Rohaninasab, Ali Bijani
Background: Bleaching may affect the bond strength of existing composite fillings and may weaken it. Hence, the aim of this study was to find the best method of in-office bleaching with the least effect on microshear bond strength (MSBS) of existing composite resin fillings to tooth structure. Materials and Methods: In this in vitro study, Class V cavities were prepared on buccal surface of 50 extracted third human sound molars. The cavities in 25 teeth had enamel axial walls, Group E, which were divided into five subgroups of E1 through E5 and in 25 teeth had dentin axial walls, Group D, which were divided into five subgroups of D1 through D5...
January 2019: Dental Research Journal
https://read.qxmd.com/read/30745522/the-precursor-osteoblast-like-cell-mc3t3-e1-cell-line-enhances-sodium-calcium-exchanger-1-ncx1-gene-expression-by-stretch-stimuli-prior-to-osteoblast-differentiation
#18
Katsuhiko Warita, Ryo Aoki, Naoki Kitamura, Izumi Shibuya, Yoshinao Z Hosaka
This study evaluated the expression of genes involved in the concentration of Ca2+ in precursor osteoblast-like cell, MC3T3-E1 subjected to stretching stimuli. Transient receptor potential vanilloid 4 (Trpv4) gene expression, the factor that is activated by stretch stimulation and enables inflow of Ca2+ from the extracellular space, was not affected as a result of stretch stimulation; conversely, the expression of sodium-calcium exchanger 1 (Ncx1) gene involved in outflow of intracellular Ca2+ increased, depending on stimulation intensity...
February 8, 2019: Journal of Veterinary Medical Science
https://read.qxmd.com/read/30743068/nano-silver-hydroxyapatite-based-antibacterial-3d-scaffolds-of-gelatin-alginate-poly-vinyl-alcohol-for-bone-tissue-engineering-applications
#19
Rajesh Kumar Saini, Laxami Prasad Bagri, A K Bajpai
In the present study macroporous three dimensional spongy scaffolds composed of gelatin, alginate, and poly (vinyl alcohol) were prepared by cryogelation technique and silver hydroxyapatite was reinforced into the 3 D matrix. The polymer nanocomposite materials were characterized by analytical techniques such as Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and x-ray diffraction (XRD) analysis. The nanocomposite scaffolds were studied for their porous nature, water sorption capacity, and mechanical behavior...
January 31, 2019: Colloids and Surfaces. B, Biointerfaces
https://read.qxmd.com/read/30742978/development-of-metformin-chain-extended-polyurethane-elastomers-as-bone-regenerative-films
#20
Betul Suyumbike Yagci, Sedat Odabas, Eda Ayse Aksoy
In this study, novel elastomeric biodegradable bone regenerative films were developed from metformin (Met) and polyurethane (PU). Metformin was selected due to its osteogenic properties and proper chemical structure to react with PU prepolymer. Metformin was integrated into PU macromolecular structure as chain extender after the synthesis of PU prepolymer via condensation polymerization of polycaprolactone diol and hexamethylene diisocyanate. Chemical, thermal, viscoelastic properties of PU-Met films where characterized and discussed in terms of structure-property relationships...
February 8, 2019: European Journal of Pharmaceutical Sciences
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