Jiapeng Ye, Zhen Hua, Jirimutu Xiao, Yang Shao, Shaoshuo Li, Heng Yin, Mao Wu, Yi Rong, Bowen Hong, Yang Guo, Yong Ma, Jianwei Wang
BACKGROUND: A series of previous investigations have revealed that p-Smad3 plays a facilitative role in the differentiation and maturation of osteoblasts, while also regulating the expression of certain intercellular communication factors. However, the effects of p-Smad3 in osteoblasts before and after maturation on the proliferation, migration, differentiation, apoptosis and other cellular behaviors of osteoclasts have not been reported. METHODS: MC3T3-E1 cells were cultured in osteogenic induction medium for varying durations, After that, the corresponding conditioned medium was collected and the osteoclast lineage cells were treated...
April 17, 2024: Molecular Biology Reports