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https://read.qxmd.com/read/30735840/new-red-shifted-fluorescent-biosensor-for-monitoring-intracellular-redox-changes
#1
Claudia Vanesa Piattoni, Florencia Sardi, Florencia Klein, Sergio Pantano, Mariela Bollati-Fogolin, Marcelo Comini
Maintenance of intracellular redox homeostasis is critical for cell survival, proliferation, differentiation, and signaling. In this regard, major changes in the intracellular redox milieu may lead to cell death whereas subtle increases in the level of certain oxidizing species may act as signals that regulate a plethora of cellular processes. Redox-sensitive variants of green fluorescent proteins (roGFP2 and rxYFP) were developed and proved useful to monitor intracellular redox changes in a non-invasive and online manner...
February 5, 2019: Free Radical Biology & Medicine
https://read.qxmd.com/read/30703768/the-effect-of-the-prethanol-extract-of-trifolium-pratense-leaves-on-interleukin-1%C3%AE-induced-cartilage-matrix-degradation-in-primary-rat-chondrocytes
#2
Seul Ah Lee, Sung-Min Moon, Seul Hee Han, Jae-Sung Kim, Do Kyung Kim, Chun Sung Kim
BACKGROUND: Osteoarthritis (OA) is a degenerative joint disease, characterized by cartilage degradation and inflammation. The proinflammatory cytokine, interleukin (IL)-1β, plays a crucial role in the pathogenesis of OA by inducing the release of other catabolic factors that contribute to cartilage degradation. Trifolium pratense L. (red clover) has been used as a medicinal plant in many countries and as a source of nutraceuticals to alleviate the symptoms of menopause. Ob-jectives: In this study, we aimed to evaluate the anticatabolic effect of 40% prethanol extract of T...
January 31, 2019: Cells, Tissues, Organs
https://read.qxmd.com/read/30681888/mgl2-is-a-hypothetical-methyltransferase-involved-in-exopolysaccharide-production-biofilm-formation-and-motility-in-rhizobium-leguminosarum-bv-trifolii
#3
Małgorzata Marczak, Kamil Żebracki, Piotr Koper, Anna Turska-Szewczuk, Andrzej Mazur, Jerzy Wydrych, Magdalena Wójcik, Anna Skorupska
In this study, functional characterization of the mgl2 gene located closely to the Pss-I exopolysaccharide biosynthesis region in Rhizobium leguminosarum bv. trifolii TA1 was described. The hypothetical protein encoded by the mgl2 gene was found to be similar to methyltransferases. Protein homology/template-based modeling facilitated prediction of the Mgl2 structure, which vastly resembled class I methyltransferases with a SAM-binding cleft. The Mgl2 protein was engaged in exopolysaccharide but not lipopolysaccharide synthesis...
January 25, 2019: Molecular Plant-microbe Interactions: MPMI
https://read.qxmd.com/read/30673406/effects-of-sublabeled-rates-of-dazomet-and-metam-sodium-applied-under-low-permeability-films-on-calonectria-microsclerotia-survival
#4
Dalia Aiello, Alessandro Vitale, Rafael F Alfenas, Acelino C Alfenas, Gabriella Cirvilleri, Giancarlo Polizzi
Infested soil is the primary inoculum source for Calonectria spp. for initiating disease in ornamental and forestry crops. The effects of dazomet and metam-sodium on survival of microsclerotia of 28 isolates belonging to 19 Calonectria spp. were evaluated in this study under nursery conditions. Two experiments with exotic Calonectria spp. in plastic containers in a greenhouse and three trials with endemic species in field plots were performed during different seasons. The containers and plots were artificially infested with Calonectria microsclerotia differentiated on carnation leaf tissues...
April 2018: Plant Disease
https://read.qxmd.com/read/30652129/genetically-encoded-fluorescent-indicators-for-imaging-intracellular-potassium-ion-concentration
#5
Yi Shen, Sheng-Yi Wu, Vladimir Rancic, Abhi Aggarwal, Yong Qian, Shin-Ichiro Miyashita, Klaus Ballanyi, Robert E Campbell, Min Dong
Potassium ion (K+ ) homeostasis and dynamics play critical roles in biological activities. Here we describe three genetically encoded K+ indicators. KIRIN1 (potassium (K) ion ratiometric indicator) and KIRIN1-GR are Förster resonance energy transfer (FRET)-based indicators with a bacterial K+ binding protein (Kbp) inserting between the fluorescent protein FRET pairs mCerulean3/cp173Venus and Clover/mRuby2, respectively. GINKO1 (green indicator of K+ for optical imaging) is a single fluorescent protein-based K+ indicator constructed by insertion of Kbp into enhanced green fluorescent protein (EGFP)...
2019: Communications biology
https://read.qxmd.com/read/30579257/improved-solubility-and-increased-biological-activity-of-neosol-%C3%A2-rcl40-a-novel-red-clover-isoflavone-aglycones-extract-preparation
#6
Marco Cosentino, Franca Marino, Emanuela Rasini, Massimiliano Legnaro, Raffaella Bombelli, Alessandra Luini, Barbara Pacchetti
Red clover (Trifolium pratense L., Fabaceae; RCL), a perennial plant rich in isoflavones, is a natural alternative for menopausal symptoms, as well as antiaging and antioxidant. Isoflavone preparations usually contain aglycones and β-glycosides. Aglycones, the active moieties, are absorbed slowly and unevenly due to reduced water solubility and biotransformation from β-glycosides. NeoSol™ RCL40 is a novel RCL isoflavone aglycones preparation based on active solubilization technologies. In the present study, NeoSol™ RCL40 was shown to induce solubilization of isoflavones and to increase estrogenic and antioxidative effects in comparison to a standard RCL extract (RCLE)...
December 19, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://read.qxmd.com/read/30579256/the-effect-of-formononetin-on-the-proliferation-and-migration-of-human-umbilical-vein-endothelial-cells-and-its-mechanism
#7
Chunmei Liang, Anwei Zhou, Chengliang Sui, Zhaoquan Huang
Formononetin is one of the main active components of traditional Chinese medicine red clover (Trifolium pratense L). Prior studies have demonstrated that formononetin is a typical phytoestrogen and exhibits an estrogen-like effect that protects the cardiovascular system with minimal side effects. In this study, we further investigated the role of formononetin in human umbilical vein endothelial cells (HUVECs) and its potential molecular mechanisms. We founded that formononetin promotes proliferation and migration of HUVECs as assessed by the MTT and wound healing assays...
December 19, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://read.qxmd.com/read/30565534/nitrogen-and-fatty-acid-rumen-metabolism-in-cattle-offered-high-or-low-polyphenol-oxidase-red-clover-silage
#8
M R F Lee, R Fychan, J K S Tweed, N Gordon, V Theobald, R Yadav, A Marshall
Polyphenol oxidase (PPO) in red clover (RC) has been shown to reduce both lipolysis and proteolysis in silo and implicated (in vitro) in the rumen. However, all in vivo comparisons have compared RC with other forages, typically with lower levels of PPO, which brings in other confounding factors as to the cause for the greater protection of dietary nitrogen (N) and C18 polyunsaturated fatty acids (PUFA) on RC silage. This study compared two RC silages which when ensiled had contrasting PPO activities (RC+ and RC-) against a control of perennial ryegrass silage (PRG) to ascertain the effect of PPO activity on dietary N digestibility and PUFA biohydrogenation...
December 19, 2018: Animal: An International Journal of Animal Bioscience
https://read.qxmd.com/read/30556796/grazing-multispecies-swards-improves-ewe-and-lamb-performance
#9
C Grace, M B Lynch, H Sheridan, S Lott, R Fritch, T M Boland
A two-year (2015 and 2016) grazing study was established to compare ewe and lamb performance when grazed on a perennial ryegrass only sward compared to more diverse sward types. In that study four sward types were investigated: a perennial ryegrass (Lolium perenne) only sward receiving 163 kg nitrogen per hectare per year (N/ha/yr) (PRG); a perennial ryegrass and white clover (Trifolium repens) sward receiving 90 kg N/ha/yr (PRGWC); a six species sward (two grasses (perennial ryegrass and timothy (Phleum pratense)), two legumes (white and red clover (Trifolium pratense)) and two herbs (ribwort plantain (Plantago lanceolata) and chicory (Cichorium intybus)) receiving 90 kg N/ha/yr (6S); and a nine species sward containing cocksfoot (Dactylis glomerata), greater birdsfoot trefoil (Lotus pedunculatus) and yarrow (Achillea millefolium) in addition to the six species listed above, receiving 90 kg N/ha/yr (9S)...
December 17, 2018: Animal: An International Journal of Animal Bioscience
https://read.qxmd.com/read/30477428/analysis-of-transcripts-and-splice-isoforms-in-red-clover-trifolium-pratense-l-by-single-molecule-long-read-sequencing
#10
Yuehui Chao, Jianbo Yuan, Sifeng Li, Siqiao Jia, Liebao Han, Lixin Xu
BACKGROUND: Red clover (Trifolium pratense L.) is an important cool-season legume plant, which is the most widely planted forage legume after alfalfa. Although a draft genome sequence was published already, the sequences and completed structure of mRNA transcripts remain unclear, which limit further explore on red clover. RESULTS: In this study, the red clover transcriptome was sequenced using single-molecule long-read sequencing to identify full-length splice isoforms, and 29,730 novel isoforms from known genes and 2194 novel isoforms from novel genes were identified...
November 26, 2018: BMC Plant Biology
https://read.qxmd.com/read/30468138/growth-performance-and-carcass-traits-of-steers-finished-on-three-different-systems-including-legume-grass-pasture-and-grain-diets
#11
A P B Fruet, F S Stefanello, F Trombetta, A N M De Souza, A G Rosado Júnior, C J Tonetto, J L C Flores, R B Scheibler, R M Bianchi, P S Pacheco, A De Mello, J L Nörnberg
Inclusion of legume in grass pastures optimizes protein values of the forage and promotes improved digestibility. Therefore, we hypothesized that finishing steers on a novel combination of legumes and grass pasture would produce carcasses with acceptable traits when compared to carcasses from steers finished in feedlot systems. In this study, we evaluated the effects of finishing steers on three systems including: grazing legume-grass pasture containing oats, ryegrass, white and red clover (PAST), grazing PAST plus supplementation with whole corn grain (14 g/kg BW (SUPP)), and on a feedlot-confined system with concentrate only (28 g/kg BW, consisting of 850 g/kg of whole corn grain and 150 g/kg of protein-mineral-vitamin supplement (GRAIN)) on growth performance of steers, carcass traits and digestive disorders...
November 23, 2018: Animal: An International Journal of Animal Bioscience
https://read.qxmd.com/read/30408053/temporal-changes-in-genetic-diversity-and-forage-yield-of-perennial-ryegrass-in-monoculture-and-in-combination-with-red-clover-in-swards
#12
Christophe Verwimp, Tom Ruttink, Hilde Muylle, Sabine Van Glabeke, Gerda Cnops, Paul Quataert, Olivier Honnay, Isabel Roldán-Ruiz
Agricultural grasslands are often cultivated as mixtures of grasses and legumes, and an extensive body of literature is available regarding interspecific interactions, and how these relate to yield and agronomic performance. However, knowledge of the impact of intraspecific diversity on grassland functioning is scarce. We investigated these effects during a 4-year field trial established with perennial ryegrass (Lolium perenne) and red clover (Trifolium pratense). We simulated different levels of intraspecific functional diversity by sowing single cultivars or by combining cultivars with contrasting growth habits, in monospecific or bispecific settings (i...
2018: PloS One
https://read.qxmd.com/read/30363087/bioconversion-of-5-deoxystrigol-stereoisomers-to-monohydroxylated-strigolactones-by-plants
#13
Kotomi Ueno, Hitomi Nakashima, Masaharu Mizutani, Hirosato Takikawa, Yukihiro Sugimoto
The bioconversion of 5-deoxystrigol (5DS) and 4-deoxyorobanchol (4DO), the simplest canonical strigolactones (SLs), into monohydroxylated SLs such as strigol, sorgomol and orobanchol was confirmed by administering of stable isotope-labeled substrates to hydroponically grown plants. Liquid chromatography-mass spectrometry analyses established that 5DS was stereoselectively converted into strigol and sorgomol by cotton ( Gossypium hirsutum ) and Chinese milk vetch ( Astragalus sinicus ), respectively. 4DO was converted into orobanchol by rice ( Oryza sativa )...
August 20, 2018: Journal of Pesticide Science
https://read.qxmd.com/read/30335760/effects-of-red-clover-isoflavones-on-tall-fescue-seed-fermentation-and-microbial-populations-in-vitro
#14
Emily A Melchior, Jason K Smith, Liesel G Schneider, J Travis Mulliniks, Gary E Bates, Zachary D McFarlane, Michael D Flythe, James L Klotz, Jack P Goodman, Huihua Ji, Phillip R Myer
Negative impacts of endophyte-infected Lolium arundinaceum (Darbyshire) (tall fescue) are responsible for over $2 billion in losses to livestock producers annually. While the influence of endophyte-infected tall fescue has been studied for decades, mitigation methods have not been clearly elucidated. Isoflavones found in Trifolium pratense (red clover) have been the subject of recent research regarding tall fescue toxicosis mitigation. Therefore, the aim of this study was to determine the effect of ergovaline and red clover isoflavones on rumen microbial populations, fiber degradation, and volatile fatty acids (VFA) in an in vitro system...
2018: PloS One
https://read.qxmd.com/read/30326221/formononetin-and-biochanin-a-protects-against-ritonavir-induced-hepatotoxicity-via-modulation-of-nf%C3%AE%C2%BAb-pakt-signaling-molecules
#15
Alauddin, Swati Chaturvedi, Mohd Yaseen Malik, Lubna Azmi, Ila Shukla, Zaiba Naseem, ChandanaVenkateswara Rao, Naresh Kumar Agarwal
AIMS: Ritonavir (RIT) is a human immune deficiency virus (HIV) protease inhibitor (PI) active against HIV-1 and HIV-2. Among various adverse effects of PIs, hepatotoxicity is a very common adverse reaction of RIT which is concentration dependent. Red clover isoflavones are found to possess anti-inflammatory, antioxidant and anti-apoptosis activity. Furthermore, recent studies have demonstrated that these isoflavones can be used to alleviate the side-effects of drugs. Hence, the present study was inquested to ascertain the effect of Formononetin (FMN) and Biochanin A (BCA) on RIT induced hepatotoxicity...
October 13, 2018: Life Sciences
https://read.qxmd.com/read/30283417/using-omic-approaches-to-compare-temporal-bacterial-colonization-of-lolium-perenne-lotus-corniculatus-and-trifolium-pratense-in-the-rumen
#16
Christopher L Elliott, Joan E Edwards, Toby J Wilkinson, Gordon G Allison, Kayleigh McCaffrey, Mark B Scott, Pauline Rees-Stevens, Alison H Kingston-Smith, Sharon A Huws
Understanding rumen plant-microbe interactions is central for development of novel methodologies allowing improvements in ruminant nutrient use efficiency. This study investigated rumen bacterial colonization of fresh plant material and changes in plant chemistry over a period of 24 h period using three different fresh forages: Lolium perenne (perennial ryegrass; PRG), Lotus corniculatus (bird's foot trefoil; BFT) and Trifolium pratense (red clover; RC). We show using 16S rRNA gene ion torrent sequencing that plant epiphytic populations present pre-incubation (0 h) were substantially different to those attached post incubations in the presence of rumen fluid on all forages...
2018: Frontiers in Microbiology
https://read.qxmd.com/read/30272805/cadmium-uptake-by-ryegrass-and-ryegrass-clover-mixtures-under-different-liming-rates
#17
Ebrahim Benyas, Jennifer Owens, Salome Seyedalikhani, Brett Robinson
Cadmium accumulates in soils that receive repeated applications of Cd-rich superphosphate fertilizers. There is evidence that adding clovers to a crop solubilizes soil Cd, increasing the bioavailability of Cd. This can lead to high plant Cd concentrations. This research aimed to test whether liming-induced increases in pH in mixed crops of clovers and ryegrasses reduced forage Cd concentrations. A greenhouse pot trial applied lime at three rates (0, 1, and 2% of soil dry weight) to eight different plant treatments-four as monocultures (perennial ryegrass [ L...
September 2018: Journal of Environmental Quality
https://read.qxmd.com/read/30269660/effects-of-red-clover-on-perimenopausal-and-postmenopausal-women-s-blood-lipid-profile-a-meta-analysis
#18
 Luís, F Domingues, L Pereira
The study aimed to perform a systematic review with meta-analysis of randomized controlled trials (RCTs) to access the effects of red clover isoflavones on the blood lipid profile of both perimenopausal and postmenopausal women. PubMed, Web of Science, Scopus, SciELO, and Cochrane Library were searched for the terms 'red clover', 'Trifolium pratense', and 'randomized controlled trial' during November 2017. Summary measures were reported as weighted mean differences (WMD) with 95% confidence interval (CI). Fixed or random effects models were used for meta-analyses, according to heterogeneity...
October 2018: Climacteric: the Journal of the International Menopause Society
https://read.qxmd.com/read/30261641/binding-of-red-clover-isoflavones-to-actin-as-a-potential-mechanism-of-anti-metastatic-activity-restricting-the-migration-of-cancer-cells
#19
Grażyna Budryn, Joanna Grzelczyk, Horacio Pérez-Sánchez
Actin functions are crucial for the ability of the cell to execute dynamic cytoskeleton reorganization and movement. Nutraceuticals that form complexes with actin and reduce its polymerization can be used in cancer therapy to prevent cell migration and metastasis of tumors. The aim of this study was to evaluate the ability of isoflavones to form complexes with actin. Docking simulation and isothermal titration calorimetry were used for this purpose. The formation of complexes by hydrogen bonds, hydrophobic and π-π interactions was demonstrated...
September 26, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://read.qxmd.com/read/30250677/annual-flower-strips-support-pollinators-and-potentially-enhance-red-clover-seed-yield
#20
Maj Rundlöf, Ola Lundin, Riccardo Bommarco
Ecological intensification provides opportunity to increase agricultural productivity while minimizing negative environmental impacts, by supporting ecosystem services such as crop pollination and biological pest control. For this we need to develop targeted management solutions that provide critical resources to service-providing organisms at the right time and place. We tested whether annual strips of early flowering phacelia Phacelia tanacetifolia support pollinators and natural enemies of seed weevils Protapion spp...
August 2018: Ecology and Evolution
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