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Fuhou Li, Zitong Ding, Wencan Ke, Dongmei Xu, Ping Zhang, Jie Bai, Shah Mudassar, Ishaq Muhammad, Xusheng Guo
The effects of Acremonium cellulase and L. plantarum A1 with ferulic acid esterase activity on corn stalk silage fermentation characteristics, carbohydrate composition and enzymatic saccharification were studied at 25 and 40 °C, respectively. Corn stalk was ensiled without additive (C), Acremonium cellulase (AC), L. plantarum A1 (Lp) and AC + Lp for 60 days. Pretreatment with Lp or AC + Lp promoted the better silage fermentation and the degradation of lignocellulose as indicated by high lactic acid and low pH and lignocellulose content compared to control silages at 25 °C...
March 6, 2019: Bioresource Technology
Susu Zhang, Zhenshang Xu, Ting Wang, Jian Kong
Endoglucanase, an important component of cellulases, is used as additives in ensiling of forage crops. However, its detailed role is unclear in ensilages. In the present study, two endoglucanases Cel5 and Cel9 produced by strain Paenibacillus panacisoli SDMCC050309, previously isolated from ensiled corn stover, were identified in the cultures by microcrystalline cellulose absorption coupled with zymogram analysis. After heterologously expressed in Escherichia coli DE3 and purified, these two proteins were biochemically characterized...
March 7, 2019: Journal of Biotechnology
Ying-Chao Zhang, Dong-Xia Li, Xue-Kai Wang, Yan-Li Lin, Qing Zhang, Xiao-Yang Chen, Fu-Yu Yang
The objective of this study was to examine the fermentation quality and aerobic stability of mulberry (Morus alba L.) silage prepared with lactic acid bacteria (LAB) and propionic acid (PA). The selected LAB strains Lactobacillus (L.) plantarum LC365281 (L1) and L. brevis LC365282 (L2), and commercial inoculant strains L. plantarum Gaofuji (GF) and L. buchneri Fresh (FR), and PA were used as additives for silage preparation. Silage treatments were designed as control, L1, L2, GF, FR, PA, PA + L1, PA + L2, PA + GF, or PA + FR...
February 17, 2019: Animal Science Journal
Leandro Janke, Bernadette Kathleen McCabe, Peter Harris, Andrew Hill, Seonmi Lee, Sören Weinrich, Serhiy Marchuk, Craig Baillie
Ensiling of sugarcane trash (SCT) and sugarcane stalks (SCS) was studied to assess the effects of molasses (MOL) and lactic acid bacteria (LAB) inoculant on methane potential. The experiment was run for 70 days and monitoring parameters were analyzed at days 0, 5, 15 and 70. Biochemical methane potential (BMP) tests performed with fresh and ensiled material at day 70 showed an increase in methane potential by 24.0%, 23.4%, 1.7% and 71.1% for SCSctr , SCTctr , SCTmol and SCTmol + lab , respectively. Such improved performance is explained by the formation of organic acids (mostly acetate and lactate) which were able to decrease the pH of the silages from 5...
January 31, 2019: Bioresource Technology
G Terler, L Gruber, W Knaus
The feed value of whole crop maize silage (WCMS) depends on nutrient composition, ruminal degradability and whole tract digestibility. However, as the ruminal degradation rate is involved in physical regulation of feed intake, ruminal degradability of WCMS may also affect feed intake and milk production of dairy cows. Thus, the aim of this study was to examine relationships between nutrient composition, ruminal degradability, and whole tract digestibility of WCMS and feed intake and milk production of dairy cows...
February 12, 2019: Animal: An International Journal of Animal Bioscience
Viet Ha Vu, Xiyang Li, Mengyuan Wang, Rongmei Liu, Guojian Zhang, Wei Liu, Baixue Xia, Qun Sun
Objective: This study aimed to gain deeper insights into the dynamic changes in spoilage fungi with fermentation and the influence of traditional additives on silage quality. Methods: Elephant grass (Pennisetum purpureum) was prepared without any additive (control), and with the addition of 0.5 % salt, and 0.5 % salt-0.2 % sugar mixture. The fungal community was then determined using a classic culturing method and high-throughput sequencing at 0, 5, 15, and 60 days after ensiling...
February 7, 2019: Asian-Australasian Journal of Animal Sciences
Rasiel Restelatto, Charles O Novinski, Lucelia M Pereira, Eduardo P A Silva, Denise Volpi, Maity Zopollatto, Patrick Schmidt, Antonio P Faciola
The objective of this study was to evaluate the effects of Lactobacillus inoculants on fermentation, losses, and aerobic stability of a total mixed ration (TMR) silage. A TMR, formulated to meet the requirements of dairy cows producing 25 kg of milk/d, was applied with the following treatments prior to ensiling: 1) Control (CON), 2) Lactobacillus buchneri (10 5 cfu/g of fresh forage; LB), and 3) Lactobacillus plantarum (10 5 cfu/g of fresh forage; LP). TMR silages were ensiled for 15 and 60 d in silos equipped with an apparatus for determination of gravimetric DM, gas, and effluent losses...
February 3, 2019: Journal of Animal Science
Mette Lübeck, Peter Stephensen Lübeck
Lactic acid bacteria (LAB) have extensive industrial applications as producers of lactic acid, as probiotics, as biocontrol agents and as biopreservatives. LAB play a large role in food fermentation and in silage processes where crops such as grass, legumes, cereals or corn are fermented into high-moisture feed that is storable and can be used to feed cattle, sheep or other ruminants. LAB also have great applications within green biorefineries, with simultaneous production of protein-rich feed for monogastric animals, silage or feed pellets for ruminants and production of lactic acid or specific amino acids...
January 30, 2019: FEMS Microbiology Letters
Shanwei Xu, Jin Li Yang, Meng Qi, Brenda Smiley, William Rutherford, Yuxi Wang, Tim A McAllister
The objective of this study was to assess the impact of Saccharomyces cerevisiae in combination with Lactobacillus buchneri on the fermentation characteristics, aerobic stability, nutritive value and microbial communities of corn silage. Whole crop corn (39% DM) was either uninoculated (Control) or inoculated with S. cerevisiae and L. buchneri at the following concentrations: S. cerevisiae 104 CFU g-1 fresh forage (S4), S. cerevisiae 105 CFU g-1 (S5), S. cerevisiae 104 CFU g-1 + L. buchneri 105 CFU g-1 (S4L5) and S...
January 31, 2019: Journal of Animal Science
S C Valadares Filho, L D Silva, O G Pereira, J Ps Roseira, M Cn Agarussi, V P Silva, T C Silva, E S Leandro, R A Paula, S A Santos, K G Ribeiro
BACKGROUND: Sugarcane silage has been increasing as a feed in the tropics by dairy farmers. However, sugarcane normally had high yeast population that leads to intense alcoholic fermentation and excessive dry-matter (DM) loss during ensilage and after air exposure, as well. OBJECTIVE: This study aimed to investigate the changes in pH, DM, water-soluble carbohydrates (WSC) and fermentation end product concentrations that occur in sugarcane silage with or without inoculation with L...
January 27, 2019: Recent Patents on Food, Nutrition & Agriculture
M Teresa Pacheco, Mar Villamiel, Rodrigo Moreno, F Javier Moreno
In this work, the efficient extraction of pectin from sugar beet by-products (pressed, ensiled and dried pulp), by using an acid method or a commercial cellulose, is accomplished. The extraction method had an impact on the pectin monomeric composition, mainly in xylose, arabinose, and galacturonic acid content, as determined by GC-FID. FTIR and SEC analyses allowed the determination of similar degrees of methoxylation and molecular weights, respectively, in the extracted pectins. The acid extraction of pectin in the ensiled by-product led to the highest yield (19%) with a galacturonic acid content of 46%, whereas the application of the enzymatic extraction method resulted in a lower yield (13%) but higher galacturonic acid content (72%)...
January 22, 2019: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Y Yang, G Ferreira, B A Corl, B T Campbell
The objective of this study was to evaluate the effects of supplementing xylanase on production performance, nutrient digestibility, and milk fatty acid profile in high-producing dairy cows consuming corn silage- or sorghum silage-based diets. Conventional corn (80,000 seeds/ha) and brown midrib forage sorghum (250,000 seeds/ha) were planted, harvested [34 and 32% of dry matter (DM), respectively], and ensiled for more than 10 mo. Four primiparous and 20 multiparous Holstein cows were randomly assigned to 1 of 4 diets in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments and 19-d periods...
January 10, 2019: Journal of Dairy Science
Daniel L Grooms, Steven R Bolin, Jessica L Plastow, Ailam Lim, Joseph Hattey, Phillip T Durst, Steven R Rust, Michael S Allen, Daniel D Buskirk, Richard W Smith
OBJECTIVE To determine whether Mycobacterium bovis remains viable in ensiled forages. SAMPLE Alfalfa, mixed mostly grass, and corn silages. PROCEDURES For each of 10 sampling days, six 250-g replicate samples of each feedstuff were created and placed in a film pouch that could be vacuum sealed to simulate the ensiling process. Within each set of replicate samples, 4 were inoculated with 10 mL of mycobacterial liquid culture medium containing viable M bovis and 2 were inoculated with 10 mL of sterile mycobacterial liquid culture medium (controls) on day 0...
January 2019: American Journal of Veterinary Research
Lili Yang, Xianjun Yuan, Junfeng Li, Zhihao Dong, Tao Shao
To reveal the mechanism of the survival and adaption of inoculated Lactobacillus plantarum during ensiling. Alfalfa was ensiled directly (A1), after γ-ray irradiation (A0), and after inoculation of the sterile (A0L) or fresh alfalfa (A1L) with Lactobacillus plantarum. The A0L had the higher lactic acid content and lower pH than that in A1L from 3 days of ensiling. Pediococcus was the dominant microbes in A1 silage, followed by Enterococcus and Lactobacillus, while Lactobacillus in A1L outnumbered all other genera at 3 d...
December 21, 2018: Bioresource Technology
Francesco Ferrero, Simona Prencipe, Davide Spadaro, Maria Lodovica Gullino, Laura Cavallarin, Serenella Piano, Ernesto Tabacco, Giorgio Borreani
The growth of Aspergillus flavus and the production of aflatoxins (AF) during the aerobic deterioration of corn silage represent a problem for animal and human health. This experiment was conducted to evaluate whether the presence of A. flavus and AF production originate from the field or additional AF are produced during the fermentation phase or during aerobic deterioration of corn silage. The trial was carried out in northern Italy on corn at a dry matter (DM) level of 34%. The fresh herbage was either not treated (C) or treated with a Lactobacillus buchneri (LB) NCIMB 40788 [(at 3 × 105 cfu/g of fresh matter (FM)], Lactobacills hilgardii (LH) CNCM I-4785 (at 3 × 105 cfu/g of FM), or their combination (LB+LH; at 1...
December 24, 2018: Journal of Dairy Science
Anuraga Jayanegara, Tekad U P Sujarnoko, Muhammad Ridla, Makoto Kondo, Michael Kreuzer
Protein degradation during ensiling is a major problem. Tannins are known to prevent or decelerate protein degradation in the rumen and may be able to do so in silages as well. Therefore, the present evaluation aimed to analyse the influence of tannins on silage quality. This was done by integrating from all suitable experiments found in literature on the topic in a meta-analysis approach. A total of 122 datasets originating from 28 experiments obtained from 16 published articles and one own unpublished experiment were included in the database...
December 26, 2018: Journal of Animal Physiology and Animal Nutrition
M R F Lee, R Fychan, J K S Tweed, N Gordon, V Theobald, R Yadav, A Marshall
Polyphenol oxidase (PPO) in red clover (RC) has been shown to reduce both lipolysis and proteolysis in silo and implicated (in vitro) in the rumen. However, all in vivo comparisons have compared RC with other forages, typically with lower levels of PPO, which brings in other confounding factors as to the cause for the greater protection of dietary nitrogen (N) and C18 polyunsaturated fatty acids (PUFA) on RC silage. This study compared two RC silages which when ensiled had contrasting PPO activities (RC+ and RC-) against a control of perennial ryegrass silage (PRG) to ascertain the effect of PPO activity on dietary N digestibility and PUFA biohydrogenation...
December 19, 2018: Animal: An International Journal of Animal Bioscience
Seong Shin Lee, Hyuk Jun Lee, Dimas Hand Vidya Paradhipta, Young Ho Joo, Sang Bum Kim, Dong Hyeon Kim, Sam Churl Kim
Objectve: This study was conducted to estimate the temperature and microbial changes of corn silages during aerobic exposure. Methods: Kwangpyeongok (KW) and Pioneer 1543 (PI) corn hybrids were harvested at 29.7% of dry matter and chopped to 3-5 cm lengths. Homo (Lactobacillus plantarum; LP) or hetero (L. buchneri; LB) fermentative inoculants at 1.2 × 105 cfu/g of fresh forage was applied to the chopped corn forage which was then ensiled in quadruplicate with a 2 x 2 (hybrid × inoculant) treatment arrangement for 100 days...
November 27, 2018: Asian-Australasian Journal of Animal Sciences
Jie Zhao, Zhihao Dong, Junfeng Li, Lei Chen, Yunfeng Bai, Yushan Jia, Tao Shao
Objectve: This study was to evaluate the fermentation dynamics, structural and nonstructural carbohydrate composition and in vitro gas production of rice straw ensiled with lactic acid bacteria (LAB) and molasses. Methods: Fresh rice straw was ensiled in 1-L laboratory silos with no additive control (C), Lactobacillus plantarum (L), molasses (M) and molasses + L. plantarum (ML) for 6, 15, 30 and 60 days. After storage, the silages were subjected to microbial and chemical analyses as well as the further in vitro fermentation trial...
November 27, 2018: Asian-Australasian Journal of Animal Sciences
Lin Sun, Zhijun Wang, Ge Gentu, Yushan Jia, Meiling Hou, Yimin Cai
Objectve: To effectively use corn stover as animal feed, we studied the changes in microbial population and chemical composition of three cultivar corn stover during field exposure and their effects on silage fermentation and in vitro digestibility. Methods: The corn stover silages were prepared from stovers exposed in the field for 0, 7, 15, 30, 60, 90, and 180 d. The microbial composition, chemical composition, fermentation quality, and in vitro digestibility were analyzed...
November 27, 2018: Asian-Australasian Journal of Animal Sciences
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