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Robert P Gersch, Jeffrey C Raum, Catherine E Calvert, Ivona Percec
BACKGROUND: The efficacy of adipose-derived stem cells (ASCs) to improve wound healing has been extensively investigated. Unfortunately, there have been no consistent reports describing significant improvements in healing time or outcomes in large-scale clinical trials using human ASCs. Primarily, these studies examined undifferentiated ASCs as opposed to specific cells differentiated from ASCs. OBJECTIVE: Here, we examine the properties of fibroblasts differentiated from human ASCs (dFib cells) in comparison to those of primary dermal fibroblasts...
March 15, 2019: Aesthetic Surgery Journal
Hui Zheng, Lihong Qiu, Yingjun Su, Chenggang Yi
BACKGROUND: Nanofats could improve photoaging. Stromal vascular fraction (SVF) and adipose-derived stem cells (ADSCs) may play pivotal roles. However, SVFs and ADSCs in nanofats processed by conventional methods cannot be enriched. Some researchers have found that after centrifugation, the SVF/ADSC density increases from top to bottom. OBJECTIVES: We hypothesized that centrifugation can be used to obtain SVF/ADSC-concentrated nanofats that are superior to conventional nanofats in improving the photoaging of skin...
March 14, 2019: Aesthetic Surgery Journal
Philip R Cohen, Christopher P Erickson, Antoanella Calame
Painful tumors of the skin present as dermal or subcutaneous nodules. They can originate from several sources: adipose tissue, cartilage degeneration, deposition of bone or calcium, eccrine glands, fibrous tissue, infiltration of benign (endometrium) or malignant (metastatic neoplasm) tissue, muscle, nerve, or vascular structures. Although pathologic evaluation of the lesion is necessary to determine the diagnosis, it is possible to make a reasonable differential diagnosis based on knowledge of prior tumors that have appeared as tender lesions...
2019: Clinical, Cosmetic and Investigational Dermatology
Adrian Djalali-Cuevas, Sergio Garnica-Galvez, Andrea Rampin, Diana Gaspar, Ioannis Skoufos, Athina Tzora, Nikitas Prassinos, Nikolaos Diakakis, Dimitrios I Zeugolis
Tissue engineering by self-assembly allows for the fabrication of living tissue surrogates by taking advantage of the cell's inherent ability to produce and deposit tissue-specific extracellular matrix. However, the long culture periods required to build a tissue substitute in conducive to phenotypic drift in vitro microenvironments result in phenotype and function losses. Although several biophysical microenvironmental modulators (e.g., surface topography, substrate stiffness, mechanical stimulation) have been used to address these issues, slow extracellular matrix deposition remains a limiting factor in clinical translation and commercialization of such therapies...
2019: Methods in Molecular Biology
Xi Zhang, Lin Chen, Bo Xiao, Hengxin Liu, Yingjun Su
It remains unclear why obese persons displayed a slower wound healing rate than the normal. In this study, we found that has_circ_0075932, a single-exon circular RNA, was outstandingly expressed in human normal adipose tissue and overexpressed in burned skin of obese persons compared with that of non-obese persons. Circ_0075932 overexpression or silencing in dermal keratinocytes had no obvious effect on cell behaviors, unless dozens of times overexpression, since its basal expression level in keratinocytes is too low...
February 26, 2019: Biochemical and Biophysical Research Communications
Carina Nicu, Jonathan Hardman, Jennifer Pople, Ralf Paus
In murine skin dermal white adipose tissue (DWAT) undergoes fluctuations in size across the hair cycle, whereas changes in size, function and metabolism of dermal adipocytes (DAs) during the human scalp hair cycle remain unexplored. Transmission electron microscopy results suggest that, during anagen-catagen transition, human DAs co-opt the autophagy machinery to undergo lipophagy within their lipid droplets. Wholemount staining of hair follicles (HFs) and surrounding DWAT for the autophagy marker LC3B confirms the increased presence of LC3B+ lipid droplets adjacent to catagen HFs; moreover, DWAT around catagen HFs engages in greater glycerol release compared to DWAT surrounding anagen HFs...
February 18, 2019: Experimental Dermatology
Helena Debiazi Zomer, Gisele Kristina Dos Santos Varela, Priscilla Barros Delben, Diana Heck, Talita da Silva Jeremias, Andrea Gonçalves Trentin
Novel strategies combining cell therapy, tissue engineering and regenerative medicine have been developed to treat major skin wounds. Although mesenchymal stromal cells (MSCs) from different tissues have similar stem cell features, such as self-renewing mesodermal differentiation potential and expression of immunophenotypic markers, they also have distinct characteristics. Therefore, we aimed to characterize the application of MSCs derived from the dermis and adipose tissue (DSCs and ASCs, respectively) in cutaneous wound healing by in vitro approaches...
February 18, 2019: Journal of Tissue Engineering and Regenerative Medicine
Yasuhiko Kaita, Takehiko Tarui, Hideaki Yoshino, Takeaki Matsuda, Yoshihiro Yamaguchi, Takatoshi Nakagawa, Michio Asahi, Masaaki Ii
Introduction: The purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs. Methods: Full thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses...
June 2019: Regenerative Therapy
Atefeh Pesaraklou, Nasser Mahdavi-Shahri, Halimeh Hassanzadeh, Masoud Ghasemi, Mahboubeh Kazemi, Nasser Sanjar Mousavi, Maryam M Matin
Today advancements in nanotechnology have made extensive progress in tissue engineering. Application of cerium oxide nanoparticles (CeO2) has improved regenerative medicine due to their antioxidant properties. In this study, nanoparticles were used to increase the efficacy of skin substitutes. Human skin samples were decellularized using four methods and studied via histological stainings and DNA content analyses. Then CeO2 dispersing and its stability were investigated. The prepared acellular dermal matrices (ADMs) were immersed in CeO2 suspension and their effects were evaluated on growth of cultured human adipose derived-mesenchymal stem cells (hAd-MSCs) using MTT assay and histological methods...
February 12, 2019: Biomedical Materials
Lin-Gwei Wei, Hsin-I Chang, Yiwei Wang, Shan-Hui Hsu, Lien-Guo Dai, Keng-Yen Fu, Niann-Tzyy Dai
Background: A tissue-engineered skin substitute, based on gelatin ("G"), collagen ("C"), and poly(ε-caprolactone) (PCL; "P"), was developed. Method: G/C/P biocomposites were fabricated by impregnation of lyophilized gelatin/collagen (GC) mats with PCL solutions, followed by solvent evaporation. Two different GC:PCL ratios (1:8 and 1:20) were used. Results: Differential scanning calorimetry revealed that all G/C/P biocomposites had characteristic melting point of PCL at around 60 °C...
2019: PeerJ
Ji Suk Choi, Woo Lee Cho, Yeo Jin Choi, Jae Dong Kim, Hyun-A Park, Su Yeon Kim, Jae Hyung Park, Dong-Gyu Jo, Yong Woo Cho
Ultraviolet-B (UVB) irradiation causes imbalance between dermal matrix synthesis and degradation through aberrant upregulation of matrix metalloproteinases (MMPs), which leads to overall skin photoaging. We investigated the effects of extracellular vesicles (EVs) derived from human adipose-derived stem cells (HASCs) on photo-damaged human dermal fibroblasts (HDFs). EVs were isolated from conditioned media of HASCs with tangential flow filtration and characterized using transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), western blotting, micro RNA (miRNA) arrays, cytokine arrays and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)...
2019: Journal of Extracellular Vesicles
Zhu Zhu, Zhaoqi Yuan, Cheng Huang, Rui Jin, Di Sun, Jun Yang, Xusong Luo
Heterologous acellular dermal matrix (ADM) has good biocompatibility and sufficient strength for clinical use for the repair of defects, tissue filling, and resurfacing of deep wounds. However, ADM tissue has such a compact structure that it can easily result in delayed vascularization after implantation. Moreover, in spite of the low immunogenicity of heterologous ADM, it can still cause varying degrees of inflammation in the host. These two drawbacks limit the efficacy and scope of clinical applications for heterologous ADM...
January 30, 2019: Biomedical Materials
Jing Wang, Yunjun Liao, Jing Xia, Zijue Wang, Xiaopei Mo, Jingwei Feng, Yunfan He, Xihang Chen, Ye Li, Feng Lu, Junrong Cai
BACKGROUND: Hypertrophic scars cause cosmetic and functional problems for patients, and their treatment remains challenging. Mechanical micronization of adipose tissue can remove adipocytes and concentrate functional cells. Stromal vascular fraction (SVF)-gel is obtained by a series of simple mechanical processes, including shifting between syringes and centrifugation. This study aimed to assess the therapeutic effect of SVF-gel on hypertrophic scars. METHODS: A model of hypertrophic scars was established in rabbit ears...
January 24, 2019: Stem Cell Research & Therapy
Sonja Lackner, Sabrina Mörkl, Wolfram Müller, Alfred Fürhapter-Rieger, Andreas Oberascher, Michael Lehofer, Claudia Bieberger, Willibald Wonisch, Omid Amouzadeh-Ghadikolai, Maximilian Moser, Harald Mangge, Sieglinde Zelzer, Sandra Johanna Holasek
BACKGROUND & AIMS: Anorexia nervosa (AN) is a severe psychosomatic disease that seriously affects nutritional status. Therapeutic approaches primarily aim for rapid weight restoration by high caloric diets and activity restriction. This often promotes abdominal body fat gain, which potentially negatively influences the patient's compliance and increases the risk of relapse. This study focused on the evaluation of body weight and subcutaneous adipose tissue (SAT) in AN patients by novel approaches...
January 10, 2019: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
Jana Dragúňová, Peter Kabát, Valéria Cucorová, Marianna Hajská, Ján Koller
Amniotic membrane is a biological material widely used in plastic and reconstructive surgery and in ophthalmology. Due to its excellent biocompatibility and strength we tried to use it as a scaffold for the in vitro cultivation of different cell types, especially keratinocytes and limbal stem cells. It was possible to cultivate limbal stem cells and keratinocytes without using 3T3 mouse fibroblast feeder cells on deep frozen amniotic membranes. The amniotic membrane can also be used as a carrier for suspensions of different types of cells, allowing a substantial reduction of the cultivation time needed to prepare cell cultures for clinical application to burn patients...
January 10, 2019: Cell and Tissue Banking
Mariya Miteva, David Castillo, Samar Sabiq
BACKGROUND: Frontal fibrosing alopecia (FFA) is an irreversible scarring alopecia, and its incidence has reached epidemic size. Immune privilege collapse of the bulge and epithelial mesenchymal transition play a role in the pathogenesis. We have noted adipose tissue in the dermis in several specimens from FFA. OBJECTIVE: Our primary objective was to verify the presence of adipose tissue at the isthmus level in biopsies from FFA. Additional objectives included the presence of deep inflammation and position of the sweat coils...
January 3, 2019: American Journal of Dermatopathology
S J Cao, L F Wang, T Ba, X Fu, F Li, C G Hao
Objective: To explore the allogeneic mouse adipose-derived mesenchymal stem cell (ADSC)-microporous sheep acellular dermal matrix (ADM) on healing of wound with full-thickness skin defect in mouse and the related mechanism. Methods: One Kunming mouse was sacrificed by cervical dislocation to collect adipose tissue from inguinal region. Mouse ADSCs were isolated from the adipose tissue and cultured in vitro. Cells of the third passage were identified by cell adipogenic and osteogenic differentiation. The expressions of CD73, CD90, CD105, and CD34 were analyzed by flow cytometry...
December 20, 2018: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
Ilja L Kruglikov, Zhuzhen Zhang, Philipp E Scherer
Hair follicles (HFs) strongly interact with adipocytes within the dermal white adipose tissue (dWAT), suggesting a strong physiological dependence on the content of immature and mature adipocytes in this layer. This content is regulated by the proliferation and differentiation of adipocyte precursors, as well as by dedifferentiation of mature existing adipocytes. Spatially, long-range interactions between HFs and dWAT involve the exchange of extracellular vesicles which are differentially released by precursors, preadipocytes, and mature adipocytes...
December 14, 2018: Trends in Endocrinology and Metabolism: TEM
Wei-Ze Syu, Shyi-Gen Chen, James Yi-Hsin Chan, Chih-Hsin Wang, Niann-Tzyy Dai, Shih-Ming Huang
INTRODUCTION: Reconstruction of segmental peripheral nerve gap is still challenging when the autografts are unavailable owing to limited availability of donor site and functional recovery. The creation of artificial conduits composed of biological or synthetic materials is still developing. Acellular dermal matrix (ADM) has been widely studied and its extension and plasticity properties may become suitable nerve conduits under different forms of nerve gaps. Adipose-derived stem cells (ADSCs) have the potential to differentiate into various cell types of different germ layers including neural stem cells (NSCs)...
January 2019: Annals of Plastic Surgery
Marek Kucharzewski, Ewa Rojczyk, Katarzyna Wilemska-Kucharzewska, Renata Wilk, Jacek Hudecki, Marek J Los
The latest findings indicate the huge therapeutic potential of stem cells in regenerative medicine, including the healing of chronic wounds. Main stem cell types involved in wound healing process are: epidermal and dermal stem cells, mesenchymal stem cells (MSCs), endothelial progenitor cells (EPCs) and hematopoietic stem cells (HSCs). In the therapy of chronic wounds, they can be administrated either topically or using different matrix like hydrogels, scaffolds, dermal substitutes and extracellular matrix (ECM) derivatives...
January 15, 2019: European Journal of Pharmacology
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