Bin Xu, Hui Chen, Jingjing Zhang, Yanghai Cong, Li Ning, Limin Chen, Yushi Zhang, Yong Zhang, Zhanchun Song, Yuan Meng, Lianqi He, Wei-Li Liao, Ying Lu, Fengyi Zhao
INTRODUCTION: Gastric cancer is a highly heterogeneous malignant tumor of the digestive system. Anti-HER2 treatment can inhibit downstream signaling pathways and improve clinical treatment and outcomes in patients with HER2 protein overexpression. Currently, two standard methods for evaluating HER2 expression status are immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). However, these low-throughput assays often produce discordant or equivocal results. METHODS: In this study, we presented a new HER2 protein detection method based on mass spectrometry selected reaction monitoring (MS-SRM) and validated the method...
2023: Frontiers in Oncology