keyword
https://read.qxmd.com/read/38330543/the-major-inducible-small-heat-shock-protein-hsp20-3-in-the-tardigrade-ramazzottius-varieornatus-forms-filament-like-structures-and-is-an-active-chaperone
#21
JOURNAL ARTICLE
Mohammad Al-Ansari, Taylor Fitzsimons, Wenbin Wei, Martin W Goldberg, Takekazu Kunieda, Roy A Quinlan
The tardigrade Ramazzottius varieornatus has remarkable resilience to a range of environmental stresses. In this study, we have characterised two members of the small heat shock protein (sHSP) family in R. varieornatus, HSP20-3 and HSP20-6. These are the most highly upregulated sHSPs in response to a 24 h heat shock at 35 0 C of adult tardigrades with HSP20-3 being one of the most highly upregulated gene in the whole transcriptome. Both R. varieornatus sHSPs and the human sHSP, CRYAB (HSPB5), were produced recombinantly for comparative structure-function studies...
December 5, 2023: Cell Stress & Chaperones
https://read.qxmd.com/read/38329705/exceptional-quantum-efficiency-powers-biomass-production-in-halotolerant-algae-picochlorum-sp
#22
JOURNAL ARTICLE
Colin Gates, Gennady Ananyev, Fatima Foflonker, Debashish Bhattacharya, G Charles Dismukes
The green algal genus Picochlorum is of biotechnological interest because of its robust response to multiple environmental stresses. We compared the metabolic performance of P. SE3 and P. oklahomense to diverse microbial phototrophs and observed exceptional performance of photosystem II (PSII) in light energy conversion in both Picochlorum species. The quantum yield (QY) for O2 evolution is the highest of any phototroph yet observed, 32% (20%) by P. SE3 (P. okl) when normalized to total PSII subunit PsbA (D1) protein, and 80% (75%) normalized per active PSII, respectively...
February 8, 2024: Photosynthesis Research
https://read.qxmd.com/read/38319409/heat-shock-protein-22-attenuates-nerve-injury-induced-neuropathic-pain-via-improving-mitochondrial-biogenesis-and-reducing-oxidative-stress-mediated-by-spinal-ampk-pgc-1%C3%AE-pathway-in-male-rats
#23
JOURNAL ARTICLE
Longqing Zhang, Lin Liu, Danyang Li, Jiayi Wu, Shaojie Gao, Fanhe Song, Yaqun Zhou, Daiqiang Liu, Wei Mei
Heat shock protein 22 (hsp22) plays a significant role in mitochondrial biogenesis and redox balance. Moreover, it's well accepted that the impairment of mitochondrial biogenesis and redox imbalance contributes to the progress of neuropathic pain. However, there is no available evidence indicating that hsp22 can ameliorate mechanical allodynia and thermal hyperalgesia, sustain mitochondrial biogenesis and redox balance in rats with neuropathic pain. In this study, pain behavioral test, western blotting, immunofluorescence staining, quantitative polymerase chain reaction, enzyme-linked immunosorbent assay, and Dihydroethidium staining are applied to confirm the role of hsp22 in a male rat model of spared nerve injury (SNI)...
February 6, 2024: Journal of Neuroimmune Pharmacology: the Official Journal of the Society on NeuroImmune Pharmacology
https://read.qxmd.com/read/38315026/a-high-throughput-target-based-screening-approach-for-the-identification-and-assessment-of-mycobacterium-tuberculosis-mycothione-reductase-inhibitors
#24
JOURNAL ARTICLE
Natalia Smiejkowska, Lauren Oorts, Kevin Van Calster, Linda De Vooght, Rob Geens, Henri-Philippe Mattelaer, Koen Augustyns, Sergei V Strelkov, Dirk Lamprecht, Koen Temmerman, Yann G-J Sterckx, Davie Cappoen, Paul Cos
The World Health Organization's goal to combat tuberculosis (TB) is hindered by the emergence of anti-microbial resistance, therefore necessitating the exploration of new drug targets. Multidrug regimens are indispensable in TB therapy as they provide synergetic bactericidal effects, shorten treatment duration, and reduce the risk of resistance development. The research within our European RespiriTB consortium explores Mycobacterium tuberculosis energy metabolism to identify new drug candidates that synergize with bedaquiline, with the aim of discovering more efficient combination drug regimens...
February 5, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38307305/usp36-mediated-parp1-deubiquitination-in-doxorubicin-induced-cardiomyopathy
#25
JOURNAL ARTICLE
Dongchen Wang, Zihao Jiang, Junyan Kan, Xiaomin Jiang, Chang Pan, Shijie You, Ruirui Chang, Juan Zhang, Hongfeng Yang, Linlin Zhu, Yue Gu
Doxorubicin (Dox) is a potent antineoplastic agent, but its use is curtailed by severe cardiotoxicity, known as Dox-induced cardiomyopathy (DIC). The molecular mechanism underlying this cardiotoxicity remains unclear. Our current study investigates the role of Ubiquitin-Specific Protease 36 (USP36), a nucleolar deubiquitinating enzyme (DUB), in the progression of DIC and its mechanism. We found increased USP36 expression in neonatal rat cardiomyocytes and H9C2 cells exposed to Dox. Silencing USP36 significantly mitigated Dox-induced oxidative stress injury and apoptosis in vitro...
January 31, 2024: Cellular Signalling
https://read.qxmd.com/read/38303105/multiomics-analyses-reveal-the-dual-role-of-flavonoids-in-pigmentation-and-abiotic-stress-tolerance-of-soybean-seeds
#26
JOURNAL ARTICLE
Ling Jiang, Xiaofeng Yang, Xiewang Gao, Hui Yang, Shumei Ma, Shan Huang, Jianyu Zhu, Hong Zhou, Xiaohong Li, Xiaoyan Gu, Hongming Zhou, Zeya Liang, Antong Yang, Yong Huang, Mu Xiao
The color of the seed coat has great diversity and is regarded as a biomarker of metabolic variations. Here we isolated a soybean variant (BLK) from a population of recombinant inbred lines with a black seed coat, while its sibling plants have yellow seed coats (YL). The BLK and YL plants showed no obvious differences in vegetative growth and seed weight. However, the BLK seeds had higher anthocyanins and flavonoids level and showed tolerance to various abiotic stresses including herbicide, oxidation, salt, and alkalinity during germination...
February 1, 2024: Journal of Agricultural and Food Chemistry
https://read.qxmd.com/read/38301812/grass-carp-peroxiredoxin-5-and-6-mediated-autophagy-inhibit-grass-carp-reovirus-replication-and-mitigate-oxidative-stress
#27
JOURNAL ARTICLE
Qian Wang, Xinyu Liang, Hanyue Wang, Cheng Yang, Yongming Li, Lanjie Liao, Zuoyan Zhu, Yaping Wang, Libo He
Peroxiredoxins (Prxs) are a family of antioxidant enzymes crucial for shielding cells against oxidative damage from reactive oxygen species (ROS). In this study, we cloned and analyzed two grass carp peroxiredoxin genes, CiPrx5 and CiPrx6. These genes exhibited ubiquitous expression across all sampled tissues, with their expression levels significantly modulated upon exposure to grass carp reovirus (GCRV). CiPrx5 was localized in the mitochondria, while CiPrx6 was uniformly distributed in the whole cells. Transfection or transformation of CiPrx5 and CiPrx6 into fish cells or E...
January 30, 2024: Fish & Shellfish Immunology
https://read.qxmd.com/read/38299351/statement-of-retraction-the-mechanism-of-%C3%AE-2-macroglobulin-against-oxidative-stress-and-promoting-cell-proliferation-in-intervertebral-disc-degeneration
#28
(no author information available yet)
No abstract text is available yet for this article.
December 2024: Bioengineered
https://read.qxmd.com/read/38299026/altered-gene-expression-linked-to-germline-dysfunction-following-exposure-to-deet
#29
JOURNAL ARTICLE
Nara Shin, Laura I Lascarez-Lagunas, Ayana L Henderson, Marina Martínez-García, Rajendiran Karthikraj, Victor Barrera, Shannan Ho Sui, Kurunthachalam Kannan, Mónica P Colaiácovo
N,N-diethyl- meta -toluamide (DEET) is a commonly used synthetic insect repellent. Although the neurological effects of DEET have been widely investigated, its effects on the germline are less understood. Here, we show that exposure of the nematode Caenorhabditis elegans , which is highly predictive of mammalian reprotoxicity, resulting in internal DEET levels within the range detected in human biological samples, causes activation of p53/CEP-1-dependent germ cell apoptosis, altered meiotic recombination, chromosome abnormalities, and missegregation...
January 19, 2024: IScience
https://read.qxmd.com/read/38286267/discovery-of-astragaloside-iv-against-high-glucose-induced-apoptosis-in-retinal-ganglion-cells-bioinformatics-and-in-vitro-studies
#30
JOURNAL ARTICLE
Jun-Qi Li, Ya-Hui Shi, Min-Xu, Cai-Xing Shi, Teng-Wang, Ting-Hua Wang, Zhong-Fu Zuo, Xue-Zheng Liu
OBJECTIVE: To examine the therapeutic mechanism of astragaloside IV (AS-IV) in the management of retinal ganglion cell (RGC) injury induced by high glucose (HG), a comprehensive approach involving the integration of network pharmacology and conducting in vitro and in vivo experiments was utilized. METHODS: A rat model of diabetic retinopathy (DR) injury was created by administering streptozotocin through intraperitoneal injection. Additionally, a model of RGC injury induced by HG was established using a glucose concentration of 0...
January 27, 2024: Gene
https://read.qxmd.com/read/38280333/s100a4-reprofiles-lipid-metabolism-in-mast-cells-via-rage-and-ppar-%C3%AE-signaling-pathway
#31
JOURNAL ARTICLE
Mo Shihui, Yan Shirong, Li Jing, He Jingjing, Wu Tongqian, Tao Tian, Wang Chenyu, Yu Fang
S100A4 is implicated in metabolic reprogramming across various cell types and is known to propel the progression of numerous diseases including allergies. Nonetheless, the influence of S100A4 on mast cell metabolic reprogramming during allergic disorders remains unexplored. Utilizing a mast cell line (C57), cells were treated with recombinant mouse S100A4 protein, with or without a PPAR-γ agonist (ROSI) or a RAGE inhibitor (FPS-ZM1). Subsequent assessments were conducted for mast cell activation and lipid metabolism...
January 26, 2024: International Immunopharmacology
https://read.qxmd.com/read/38278996/cortistatin-prevents-glucocorticoid-associated-osteonecrosis-of-the-femoral-head-via-the-ghsr1a-akt-pathway
#32
JOURNAL ARTICLE
Yuan Gao, Yunhao You, Pengfei Zhang, Yang Yu, Zhaoning Xu, Hui Wei, Zhicheng Liu, Ruixuan Yu, Gaoxin Jin, Hao Wang, Shuai Zhang, Yuhua Li, Weiwei Li
Long-term use of glucocorticoids (GCs) is known to be a predominant cause of osteonecrosis of the femoral head (ONFH). Moreover, GCs can mediate apoptosis of various cell types by exaggerating oxidative stress. We have previously found that Cortistatin (CST) antagonizes oxidative stress and improves cell apoptosis in several conditions. In this study, we detected that the CST expression levels were diminished in patients with ONFH compared with femoral neck fracture (FNF). In addition, a GC-induced rat ONFH model was established, which impaired bone quality in the femoral head...
January 26, 2024: Communications Biology
https://read.qxmd.com/read/38277724/upregulation-of-trpc1-protects-against-high-glucose-induced-huvecs-dysfunction-by-inhibiting-oxidative-stress
#33
JOURNAL ARTICLE
Mengting Qu, Lailing Du
-To explore the effect of TRPC1 on endothelial cell function damage under a high glucose environment and its downstream molecular mechanism, and provide new theory and strategy for improving diabetic endothelial cell function and promoting vascular injury repair. In vitro, we use high glucose to treat human umbilical vein endothelial cells (HUVECs) and upregulated TRPC1 with adenovirus infection. HUVECs were split into 4 groups: (i) NG Group: Treated with normal glucose; (ii) HG Group: Treated with high glucose; (iii) HG + adGFP Group: High glucose + the control adenovirus (adGFP); (iv) HG + adTRPC1 Group: High glucose + recombinant adenovirus encoding TRPC1...
January 21, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38275638/antioxidant-defence-in-labeo-rohita-to-biotic-and-abiotic-stress-insight-from-mrna-expression-molecular-characterization-and-recombinant-protein-based-elisa-of-catalase-glutathione-peroxidase-cuzn-superoxide-dismutase-and-glutathione-s-transferase
#34
JOURNAL ARTICLE
Sonali Parida, Pramoda Kumar Sahoo
Fish possess numerous enzymatic antioxidant systems as part of their innate immunity. These systems have been poorly studied in Labeo rohita (rohu). The present study characterized and investigated the role of antioxidant genes in the defence mechanisms against two types of stressors, including infection and ammonia stress. Four key genes associated with antioxidant activity-catalase, glutathione peroxidase, glutathione S-transferase, and CuZn superoxide dismutase were successfully cloned and sequenced. These genes were found to be expressed in different tissues and developmental stages of rohu...
December 21, 2023: Antioxidants (Basel, Switzerland)
https://read.qxmd.com/read/38275172/effect-and-mechanism-of-recombinant-human-fibroblast-growth-factor-18-on-osteoporosis-in-ovx-mice
#35
JOURNAL ARTICLE
P Y Lu, M Huang, M H Shao, J X Hu, C Y Ding, Y J Feng, M Zhang, H P Lin, H S Tian
OBJECTIVES: This study aimed to investigate the effect and the mechanism of recombinant human fibroblast growth factor 18 (rhFGF18) on postmenopausal osteoporosis. METHODS: The effect of rhFGF18 on the proliferation and apoptosis of osteoblasts and the mechanism underlying such an effect was evaluated using an oxidative stress model of the MC3T3-E1 cell line. Furthermore, ovariectomy was performed on ICR mice to imitate estrogen-deficiency postmenopausal osteoporosis...
January 26, 2024: Climacteric: the Journal of the International Menopause Society
https://read.qxmd.com/read/38251374/paracrine-signaling-mediated-by-the-cytosolic-tryparedoxin-peroxidase-of-trypanosoma-cruzi
#36
JOURNAL ARTICLE
María Laura Chiribao, Florencia Díaz-Viraqué, María Gabriela Libisch, Carlos Batthyány, Narcisa Cunha, Wanderley De Souza, Adriana Parodi-Talice, Carlos Robello
Peroxiredoxins are abundant and ubiquitous proteins that participate in different cellular functions, such as oxidant detoxification, protein folding, and intracellular signaling. Under different cellular conditions, peroxiredoxins can be secreted by different parasites, promoting the induction of immune responses in hosts. In this work, we demonstrated that the cytosolic tryparedoxin peroxidase of Trypanosoma cruzi (cTXNPx) is secreted by epimastigotes and trypomastigotes associated with extracellular vesicles and also as a vesicle-free protein...
January 10, 2024: Pathogens
https://read.qxmd.com/read/38247525/c2cd4b-evokes-oxidative-stress-and-vascular-dysfunction-via-a-pi3k-akt-pkc%C3%AE-signaling-pathway
#37
JOURNAL ARTICLE
Paola Di Pietro, Angela Carmelita Abate, Valeria Prete, Antonio Damato, Eleonora Venturini, Maria Rosaria Rusciano, Carmine Izzo, Valeria Visco, Michele Ciccarelli, Carmine Vecchione, Albino Carrizzo
High glucose-induced endothelial dysfunction is an important pathological feature of diabetic vasculopathy. While genome-wide studies have identified an association between type 2 diabetes mellitus (T2DM) and increased expression of a C2 calcium-dependent domain containing 4B (C2CD4B), no study has yet explored the possible direct effect of C2CD4B on vascular function. Vascular reactivity studies were conducted using a pressure myograph, and nitric oxide and oxidative stress were assessed through difluorofluorescein diacetate and dihydroethidium, respectively...
January 14, 2024: Antioxidants (Basel, Switzerland)
https://read.qxmd.com/read/38247452/a-zero-valent-sulfur-transporter-helps-podophyllotoxin-uptake-into-bacterial-cells-in-the-presence-of-ctab
#38
JOURNAL ARTICLE
Honglei Liu, Huiyuan Yu, Rui Gao, Fulin Ge, Rui Zhao, Xia Lu, Tianqi Wang, Huaiwei Liu, Chunyu Yang, Yongzhen Xia, Luying Xun
Podophyllotoxin (PTOX) is naturally produced by the plant Podophyllum species. Some of its derivatives are anticancer drugs, which are produced mainly by using chemical semi-synthesis methods. Recombinant bacteria have great potential in large-scale production of the derivatives of PTOX. In addition to introducing the correct enzymes, the transportation of PTOX into the cells is an important factor, which limits its modification in the bacteria. Here, we improved the cellular uptake of PTOX into Escherichia coli with the help of the zero-valent sulfur transporter YedE1E2 in the presence of cetyltrimethylammonium bromide (CTAB)...
December 22, 2023: Antioxidants (Basel, Switzerland)
https://read.qxmd.com/read/38242850/localized-oxidation-embellishing-strategy-enables-high-performance-perovskite-solar-cells
#39
JOURNAL ARTICLE
Minchao Liu, Yiyang Wang, Chenxing Lu, Can Zhu, Zhe Liu, Jinyuan Zhang, Meng Yuan, Yishun Feng, Xin Jiang, Siguang Li, Lei Meng, Yongfang Li
Perovskite solar cell (pero-SC) has attracted extensive studies as a promising photovoltaic technology, wherein the electron extraction and transfer exhibit pivotal effect to the device performance. The planar SnO2 electron transport layer (ETL) has contributed the recent record power conversion efficiency (PCE) of the pero-SCs, yet still suffers from surface defects of SnO2 nanoparticles which brings energy loss and phase instability. Herein, we report a localized oxidation embellishing (LOE) strategy by applying (NH4)2CrO4 on the SnO2 ETL...
January 19, 2024: Angewandte Chemie
https://read.qxmd.com/read/38242372/protective-effects-of-metrnl-overexpression-against-pathological-cardiac-remodeling
#40
JOURNAL ARTICLE
Huang Cao, Yiming Liao, Junmou Hong
At present, meteorin-like protein (METRNL) has been proven to be widely expressed in the myocardium and participates in the pathogenic process of various cardiovascular diseases. However, the effects of METRNL on pathological cardiac hypertrophy is still unknown. In the present study, we used a mouse model of transverse aortic constriction (TAC) surgery to mimic pathological cardiac hypertrophy and gene delivery system to overexpress METRNL in vivo. The results showed that METRNL overexpression improved TAC-induced pathological cardiac hypertrophy in mice and neonatal cardiomyocytes...
January 17, 2024: Gene
keyword
keyword
166335
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.