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recombinant oxidative stress

Tyler G McCaslin, Cynthia V Pagba, San-Hui Chi, Hyea J Hwang, James C Gumbart, Joseph W Perry, Cristina Olivieri, Fernando Porcelli, Gianluigi Veglia, Zhanjun Guo, Miranda McDaniel, Bridgette A Barry
Tyrosine-tryptophan (YW) dyads are ubiquitous structural motifs in enzymes and play roles in proton-coupled electron transfer (PCET) and, possibly, protection from oxidative stress. Here, we describe the function of YW dyads in de novo designed 18-mer, β hairpins. In Peptide M, a YW dyad is formed between W14 and Y5. A UV hypochromic effect and an excitonic Cotton signal are observed, in addition to singlet, excited state (W*) and fluorescence emission spectral shifts. In a second Peptide, Peptide MW, a Y5-W13 dyad is formed diagonally across the strand and distorts the backbone...
March 19, 2019: Journal of Physical Chemistry. B
Shinichi Oide, Yuya Tanaka, Akira Watanabe, Masayuki Inui
The cell wall integrity and stress response component (WSC) domain was first described in the Wsc-family protein of the yeast Saccharomyces cerevisiae, and later found in diverse eukaryotic organisms. Due solely to their presence in the Wsc-family proteins working as a plasma membrane sensor for surface stress and in a fungal β-1,3-exoglucanse, WSC domains have been presumed to possess carbohydrate-binding property without any experimental evidence. Aiming at elucidation of function(s) of WSC domains, we characterized a WSC domain-containing alcohol oxidase from the rice blast pathogen Pyricularia oryzae (PoAlcOX)...
June 2019: Enzyme and Microbial Technology
Rubén Torres, Ester Serrano, Kristina Tramm, Juan C Alonso
Bacillus subtilis radA is epistatic to disA and recA genes in response to methyl methane sulfonate- and 4-nitroquinoline-1-oxide-induced DNA damage. We show that ΔradA cells were sensitive to mitomycin C- and H2 O2 -induced damage and impaired in natural chromosomal transformation, whereas cells lacking DisA were not. RadA/Sms mutants in the conserved H1 (K104A and K104R) or KNRFG (K255A and K255R) motifs fail to rescue the sensitivity of ΔradA in response to the four different DNA damaging agents. A RadA/Sms H1 or KNRFG mutation impairs both chromosomal and plasmid transformation, but the latter defect was suppressed by inactivating RecA...
March 4, 2019: DNA Repair
B Nandi, S Talluri, S Kumar, C Yenumula, J S Gold, R Prabhala, N C Munshi, M A Shammas
A variety of factors, whether extracellular (mutagens/carcinogens and viruses in the environment, chronic inflammation and radiation associated with the environment and/or electronic devices/machines) and/or intracellular (oxidative metabolites of food, oxidative stress due to inflammation, acid production, replication stress, DNA replication/repair errors, and certain hormones, cytokines, growth factors), pose a constant threat to the genomic integrity of a living cell. However, in the normal cellular environment multiple biological pathways including DNA repair, cell cycle, apoptosis and the immune system work in a precise, regulated (tightly controlled), timely and concerted manner to ensure genomic integrity, stability and proper functioning of a cell...
April 2019: Journal of Translational Science
Sung-Ho Park, Gi Bae Kim, Hyun Uk Kim, Si Jae Park, Jong-Il Choi
During microbial production of target product, accumulation of by-products and target product itself may be toxic to host strain. Thus, development of abiotic stress tolerant strains are essential to achieve high productivity of target product with sustained metabolism. Expression of DR1558 from Deinococcus radiodurans, a response regulator in two-component signal transduction system, was reported to increase the tolerance against oxidative stress in Escherichia coli. In this study, the effect of overexpression of DR1558 was examined on poly‑3‑hydroxybutyrate (PHB) production in recombinant E...
March 6, 2019: International Journal of Biological Macromolecules
Vijay K Sharma, Suryatej Akavaram, Robert G Schaut, Darrell O Bayles
BACKGROUND: Escherichia coli O157:H7 (O157) has been linked to numerous foodborne disease outbreaks. The ability to rapidly sequence and analyze genomes is important for understanding epidemiology, virulence, survival, and evolution of outbreak strains. In the current study, we performed comparative genomics to determine structural and functional features of the genome of a foodborne O157 isolate NADC 6564 and infer its evolutionary relationship to other O157 strains. RESULTS: The chromosome of NADC 6564 contained 5466 kb compared to reference strains Sakai (5498 kb) and EDL933 (5547 kb) and shared 41 of its 43 Linear Conserved Blocks (LCB) with the reference strains...
March 8, 2019: BMC Genomics
Zafar Iqbal, Grant Morahan, Mahreen Arooj, Alexandre N Sobolev, Shahid Hameed
A novel class of spiroimidazolidine-2',4'-diones substituted with aryl sulfonyl group at different positions was designed and synthesized. The target compounds were evaluated for their potential to release insulin from MIN6 cell line derived from in-vivo immortalized insulin-secreting pancreatic cells. The MIN6 cells represent an important model of beta cells, which as passage numbers increases, lose the first phase but retain partial second phase glucose stimulated insulin secretion (GSIS), similar to patients in early type 2 diabetes onset...
February 15, 2019: European Journal of Medicinal Chemistry
Zhenxian Zhang, Hiroki Hamada, Phillip M Gerk
Monoamine oxidases (MAOs) regulate local levels of neurotransmitters such as dopamine, norepinephrine, and serotonin and thus have been targeted by drugs for the treatment of certain CNS disorders. However, recent studies have shown that these enzymes are upregulated with age in nervous and cardiac tissues and may be involved in degeneration of these tissues, since their metabolic mechanism releases hydrogen peroxide leading to oxidative stress. Thus, targeting these enzymes may be a potential anti-aging strategy...
2019: BioMed Research International
Zhimin Huang, Qing Li, Yanggang Yuan, Chengning Zhang, Lin Wu, Xi Liu, Wei Cao, Honglei Guo, Suyan Duan, Xueqiang Xu, Bo Zhang, Changying Xing
AIMS: Acute kidney injury (AKI) can limit the clinical use of cisplatin in cancer treatment. The drivers of cisplatin-induced AKI include oxidative stress, mitochondrial dysfunction and apoptosis. Previous studies showed renalase protected cultured human renal proximal tubular cell (HK-2) against cisplatin induced necrosis, and renalase-knockout mice subjected to cisplatin showed exacerbated kidney injury. Therefore, it is necessary to determine the exact mechanisms of renalase in cisplatin-induced nephrotoxicity...
February 21, 2019: Life Sciences
Zahra Abbasi-Malati, Fatemeh Amiri, Mahshid Mohammadipour, Mehryar Habibi Roudkenar
The mammalian expression system plays a key central role in the production of therapeutic recombinant proteins. Conspicuously, any improvements in the expression system which lead to a higher expression level would have an impact especially in bio-pharmaceutical industries. In the current study, to take steps toward the improvement of expression of recombinant protein, first, we established a stable HEK293 cell line to overexpress a well-known cytoprotective and antioxidant gene, Nrf2. Next, we transiently expressed human recombinant coagulation factor VII, as an example of human recombinant protein, in the engineered-HEK293 cell line...
February 21, 2019: Molecular Biotechnology
Jamie G Hijmans, Kelly A Stockelman, Ma'ayan V Levy, Lillian M Brewster, Tyler Daniel Bammert, Jared J Greiner, Elizabeth Connick, Christopher A DeSouza
The aims of this study were two-fold. The first was to determine if human immunodeficiency virus (HIV)-1 gp120 and Tat stimulate the release of endothelial microvesicles (EMVs). The second was to determine whether viral protein induced EMVs are deleterious to endothelial cell function: inducing endothelial cell inflammation, oxidative stress and senescence, and increasing apoptotic susceptibility. Human aortic endothelial cells (HAECs) were treated with recombinant HIV-1 proteins Bal gp120 (R5), Lav gp120 (X4) or Tat...
February 21, 2019: Journal of Applied Physiology
Freda E-C Jen, Evgeny A Semchenko, Christopher J Day, Kate L Seib, Michael P Jennings
Control of the sexually transmitted infection gonorrhea is a major public health challenge, due to the recent emergence of multidrug resistant strains of Neisseria gonorrhoeae , and there is an urgent need for novel therapies or a vaccine to prevent gonococcal disease. In this study, we evaluated the methionine sulfoxide reductase (MsrA/B) of N. gonorrhoeae as a potential vaccine candidate, in terms of its expression, sequence conservation, localization, immunogenicity, and the functional activity of antibodies raised to it...
2019: Frontiers in Immunology
D Merrill Dane, Cuneyt Yilmaz, Dipendra Gyawali, Roshni Iyer, Jyothi U Menon, Kytai T Nguyen, Priya Ravikumar, Aaron S Estrera, Connie C W Hsia
Paracrine erythropoietin (EPO) signaling in the lung recruits endothelial progenitor cells, promotes cell maturation and angiogenesis , and is upregulated during canine post-pneumonectomy (PNX) compensatory lung growth. To determine whether inhalational delivery of exogenous EPO augments endogenous post-PNX lung growth, adult canines underwent right PNX and received, via a permanent tracheal stoma, weekly nebulization of recombinant human EPO-containing nanoparticles or empty nanoparticles (control) for 16 weeks...
February 20, 2019: American Journal of Physiology. Lung Cellular and Molecular Physiology
Fengmei Li, Yanjiao Liang, Jinling Cai, Yanjing Shi, Liyan Ma, Yongzhong Lu
Thioredoxin system plays an important role in antioxidative stress, thioredoxin 2 (Trx2) being one of the most important components in the thioredoxin system. The full-length cDNA sequence of thioredoxin 2 from Euphausia superba (EsTrx2) is 1276 bp and contain a 5' untranslated region (UTR) of 94 bp, a 3' UTR of 741 bp and an open reading frame (ORF) of 441 bp, encoding a putative protein of 146 amino acids. Multiple sequence alignments have indicated that EsTrx2 possesses a conserved (-Cys-Gly-Pro-Cys-) CGPC redox-active site...
February 15, 2019: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Yinan Zhou, Xin Li, Xueying Yuan, Lirong Hao
Peritoneal dialysis (PD) is often used to treat patients with end stage renal disease, and its long-term complications include excessive inflammation and oxidative stress. Allograft inflammatory factor 1 (AIF-1), as a cytoplasmic protein, is originally identified from infiltrating macrophages, and it was associated with inflammation in the cells other than macrophages, such as endothelial cells and vascular smooth muscle cells. To clarify the molecular mechanisms of AIF-1-modulated pathological changes in the peritoneum during PD, we first detected the AIF-1 expression in peritoneal tissues from PD mice...
February 13, 2019: Cell Biology International
Robert M Hoffman, Kenji Miki, Waddah Al-Refaie, Mingxu Xu, Yuying Tan
Recombinant methioninase (rMETase) derived from Pseudomonas putida targets the elevated methionine (MET) requirement of cancer cells (methionine dependence) and has shown efficacy against a variety of cancer types in mouse models. To enhance the efficacy of rMETase, we constructed the pLGFP-METSN retrovirus encoding the P. putida methioninase (METase) gene fused with the green fluorescent protein (GFP) gene. pLGFP-METSN or control vector pLGFPSN was introduced into the human lung cancer cell line H460. The retrovirus-mediated METase gene transfer decreased the intracellular MET level of the cancer cells and consequently enhanced the efficacy of treatment with the rMETase protein...
2019: Methods in Molecular Biology
Devis Pascut, Caecilia Hapsari Ceriapuri Sukowati, Giulia Antoniali, Giovanna Mangiapane, Silvia Burra, Luca Giovanni Mascaretti, Matteo Rossano Buonocore, Lory Saveria Crocè, Claudio Tiribelli, Gianluca Tell
Late diagnosis for Hepatocellular Carcinoma (HCC) remains one of the leading causes for the high mortality rate. The apurinic/apyrimidinic endonuclease 1 (APE1), an essential member of the base excision DNA repair (BER) pathway, contributes to cell response to oxidative stress and has other non-repair activities. In this study, we evaluate the role of serum APE1 (sAPE1) as a new diagnostic biomarker and we investigate the biological role for extracellular APE1 in HCC. sAPE1 level was quantified in 99 HCC patients, 50 non-HCC cirrhotic and 100 healthy controls...
January 8, 2019: Oncotarget
Ting Tang, Hehe Sun, Yongbao Li, Peiru Chen, Fengsong Liu
Rhodanese homology domains (RHODs) are the structural modules of ubiquitous tertiary that occur in three major evolutionary phyla. Despite the versatile and important physiological functions of RHODs containing proteins, little is known about their invertebrate counterparts. A novel HSP67B2-like single-domain rhodanese homologue, MdRDH1 from Musca domestica, whose expression can be induced by bacterial infection or oxidative stress. Silencing MdRDH1 through RNAi causes important accumulations of reactive oxygen species (ROS) and malondialdehyde (MDA), and increases mortality in the larvae treated with bacterial invasion...
February 1, 2019: Molecular Immunology
Bin Liu, Yumei Rong, Dan Sun, Wuwei Li, Hong Chen, Bo Cao, Taoyuan Wang
Specific study about the inhibitory effect of costunolide (CN) and relevant mechanism is of great significance for the treatment of pulmonary fibrosis. Here, the pharmacological activity of costunolide on the treatment of pulmonary fibrosis was investigated in vivo and in vitro. The in vivo mice study, mice were received intratracheal injection of bleomycin (BLM, 5 mg/kg) on 0 day to obtain BLM-induced pulmonary fibrosis firstly. From 2 day to 21 day, mice were orally administered with different dose of CN (low dose(CNL): 10 mg/kg, high dose(CNH): 20 mg/kg) and pirfenidone (PFD)(positive control, 50 mg/kg)...
March 5, 2019: Biochemical and Biophysical Research Communications
Zhihua Si, Jinzhi Liu, Ke Hu, Yan Lin, Jie Liu, Aihua Wang
Recombinant tissue plasminogen activator (rt-PA) is the first-line drug for revascularization in acute cerebral infarction (ACI) treatment. In this study, an improved rat embolic middle cerebral artery occlusion model for ischaemic stroke was used and the rats were killed on the first, third and seventh day after model establishment. Increases in infarct volume were significantly less in the thrombolytic group than in the conventional group at every time-point. The microvascular density (MVD) in the thrombolytic group was significantly higher than that in the conventional group at every time-point, especially on the seventh day...
January 29, 2019: Journal of Cellular and Molecular Medicine
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