keyword
https://read.qxmd.com/read/38518664/integrated-landscape-of-chicken-egg-chalaza-proteomics
#21
JOURNAL ARTICLE
Jing Pu, Jian Hu, Jing Xiao, Shugang Li, Beibei Wang, Jinqiu Wang, Fang Geng
Chicken egg chalaza (CLZ) is a natural colloidal structure in eggs that exists as an egg yolk stabilizer and is similar in composition to egg white. In this study, the proteome, phosphoproteome, and N-glycoproteome of CLZ were characterized in depth. We hydrolyzed the CLZ proteins and enriched the phosphopeptides and glycopeptides. We identified 45 phosphoproteins and 80 N-glycoproteins, containing 59 phosphosites and 203 N-glycosylation sites, respectively. Typically, the ovalbumin in CLZ was both phosphorylated and N-glycosylated, with 4 phosphosites and 4 N-glycosylation sites...
March 7, 2024: Poultry Science
https://read.qxmd.com/read/38518610/simultaneous-production-of-biofuel-and-removal-of-heavy-metals-using-marine-alga-turbinaria-turbinata-as-a-feedstock-in-neom-region-tabuk
#22
JOURNAL ARTICLE
Amenah S Alotaibi, Abrar M Alhumairi, Hanaa Ghabban, Asma Massad Alenzi, Ragaa A Hamouda
Depletion of fossil fuel and pollution by heavy metals are two major global issues. The cell wall of algae consists of polymers of polysaccharides such as cellulose, hemicellulose, alginate, starch, and many others that are readily hydrolyzed to monosaccharides and hence are amenable to fermentation into bioethanol. Moreover, algae contain lipids that may undergo trans-esterification to biodiesel, and can be absorbed by heavy metals. In this study, extraction of lipids from Turbinaria turbinata (common brown alga) from the beach of Sharma, NEOM, Tabuk, Saudi Arabia by different solvents hexane, methanol, and hexane: methanol (1:1), and trans-esterification was performed to obtain biodiesel and investigated by GC...
March 21, 2024: Ecotoxicology and Environmental Safety
https://read.qxmd.com/read/38518501/promoting-nitrogen-conversion-in-aerobic-biotransformation-of-swine-slurry-with-the-co-application-of-manganese-sulfate-and-biochar
#23
JOURNAL ARTICLE
Xuan Wu, Xinyu Zhao, Guorong Yi, Wanqiang Zhang, Runyu Gao, Daniel Kuok Ho Tang, Ran Xiao, Zengqiang Zhang, Yiqing Yao, Ronghua Li
This study aimed to explore the co-application of MnSO4 (Mn) and biochar (BC) in nitrogen conversion during the composting process. A 70-day aerobic composting was conducted using swine slurry, supplemented with different levels of Mn (0, 0.25%, and 0.5%) and 5% BC. The results demonstrated that the treatment with 0.5MnBC had the highest levels of NH4 + -N (3.07 g kg-1 ), TKN (29.90 g kg-1 ), and NO3 - -N (1.94 g kg-1 ) among all treatments. Additionally, the 0.5MnBC treatment demonstrated higher urease, protease, nitrate reductase, and nitrite reductase activities than the other treatments, with the peak values of 18...
March 21, 2024: Journal of Environmental Management
https://read.qxmd.com/read/38518488/performance-of-microbial-inoculation-and-tricalcium-phosphate-on-nitrogen-retention-and-conversion-core-microorganisms-and-enzyme-activity-during-kitchen-waste-composting
#24
JOURNAL ARTICLE
Taha Ahmed Mohamed, Zimin Wei, Mohamed Mohaseb, Wu Junqiu, Taha El Maghraby, Xiaomeng Chen, Yousif Abdelrahman Yousif Abdellah, Daichen Mu, Mohamed El Kholy, Chaonan Pan, Ayodeji Bello, Guangren Zheng, Ahmed Mohamed Ahmed, Marwa Ahmed, Yue Zhao
The substantial release of NH3 during composting leads to nitrogen (N) losses and poses environmental hazards. Additives can mitigate nitrogen loss by adsorbing NH3 /NH4 , adjusting pH, and enhancing nitrification, thereby improving compost quality. Herein, we assessed the effects of combining bacterial inoculants (BI) (1.5%) with tricalcium phosphate (CA) (2.5%) on N retention, organic N conversion, bacterial biomass, functional genes, network patterns, and enzyme activity during kitchen waste (KW) composting...
March 21, 2024: Journal of Environmental Management
https://read.qxmd.com/read/38517887/a-model-driven-approach-to-upcycling-recalcitrant-feedstocks-in-pseudomonas-putida-by-decoupling-pha-production-from-nutrient-limitation
#25
JOURNAL ARTICLE
Maria-Tsampika Manoli, Álvaro Gargantilla-Becerra, Carlos Del Cerro Sánchez, Virginia Rivero-Buceta, M Auxiliadora Prieto, Juan Nogales
Bacterial polyhydroxyalkanoates (PHAs) have emerged as promising eco-friendly alternatives to petroleum-based plastics since they are synthesized from renewable resources and offer exceptional properties. However, their production is limited to the stationary growth phase under nutrient-limited conditions, requiring customized strategies and costly two-phase bioprocesses. In this study, we tackle these challenges by employing a model-driven approach to reroute carbon flux and remove regulatory constraints using synthetic biology...
March 21, 2024: Cell Reports
https://read.qxmd.com/read/38515830/effect-of-sulfuric-acid-hydrolysis-on-the-structure-and-pickering-emulsifying-capacity-of-acorn-starch
#26
JOURNAL ARTICLE
Changsheng Guo, Sheng Geng, Yuzhong Shi, Chao Yuan, Benguo Liu
The acid-hydrolyzed acorn starch samples (HAS-1, HAS-2, HAS-3, and HAS-4) were prepared from natural acorn starch (NAS) at sulfuric acid concentrations of 1, 2, 3, and 4 mol/L for 2 d. The particle characteristics and structures of HAS were investigated, and Pickering high internal phase emulsions (HIPEs) based on HAS were constructed and characterized. The results showed that with an increase in sulfuric acid concentration, the size, yield, amylose content, molecular weight, and amylopectin chain length of HAS gradually decreased...
June 30, 2024: Food chemistry: X
https://read.qxmd.com/read/38515717/an-impact-of-n-glycosylation-on-biochemical-properties-of-a-recombinant-%C3%AE-amylase-from-bacillus-licheniformis
#27
JOURNAL ARTICLE
Assel Kiribayeva, Dmitriy Silayev, Zhiger Akishev, Kairat Baltin, Saniya Aktayeva, Yerlan Ramankulov, Bekbolat Khassenov
Amylases are enzymes that are known to hydrolyze starch. High efficiency of amylolytic enzymes allows them to compete in the industry with the technology of chemical hydrolysis of starch. A Bacillus licheniformis strain with high amylolytic activity was isolated from soil and designated as T5. The gene encoding α-amylase from B. licheniformis T5 was successfully expressed in both Escherichia coli (rAmyT5-E) and Pichia pastoris (as rAmyT5-P). According to the study, the recombinant α-amylases rAmyT5-E and rAmyT5-P exhibited the highest activity at pH 6...
March 30, 2024: Heliyon
https://read.qxmd.com/read/38515623/application-of-pectin-hydrolyzing-bacteria-in-tobacco-to-improve-flue-cured-tobacco-quality
#28
JOURNAL ARTICLE
Shuning Weng, Meizhong Deng, Shanyi Chen, Renqiang Yang, Jingjing Li, Xianbo Zhao, Shunhua Ji, Lixiang Wu, Li Ni, Enren Zhang, Chaochao Wang, Lingfeng Qi, Kuanqi Liao, Yiqiang Chen, Wen Zhang
To study the relationship between the diversity of the surface microbial community and tobacco flavor, and to improve tobacco quality using microorganisms. The microbial community composition and diversity of 14 samples of flue-cured tobacco from tobacco-producing areas in Yunnan with varying grades were analyzed by high-throughput sequencing. PICRUSt was used for predicting microbial functions. A strain of Bacillus amyloliquefaciens W6-2 with the ability to degrade pectin was screened from the surface of flued-cured tobacco leaves from Yunnan reroasted tobacco leave...
2024: Frontiers in Bioengineering and Biotechnology
https://read.qxmd.com/read/38515401/backflow-reduction-in-local-injection-therapy-with-gelatin-formulations
#29
JOURNAL ARTICLE
Kazuki Kotani, Francois Marie Ngako Kadji, Yoshinobu Mandai, Yosuke Hiraoka
The local injection of therapeutic drugs, including cells, oncolytic viruses and nucleic acids, into different organs is an administrative route used to achieve high drug exposure at the site of action. However, after local injection, material backflow and side effect reactions can occur. Hence, this study was carried out to investigate the effect of gelatin on backflow reduction in local injection. Gelatin particles (GPs) and hydrolyzed gelatin (HG) were injected into tissue models, including versatile training tissue (VTT), versatile training tissue tumor-in type (VTT-T), and broiler chicken muscles (BCM), using needle gauges between 23 G and 33 G...
December 2024: Drug Delivery
https://read.qxmd.com/read/38515244/genomic-basis-of-the-distinct-biosynthesis-of-%C3%AE-glucogallin-a%C3%A2-biochemical-marker-for-hydrolyzable-tannin-production-in%C3%A2-three-oak-species
#30
JOURNAL ARTICLE
Qinsong Yang, Jinjin Li, Yan Wang, Zefu Wang, Ziqi Pei, Nathaniel R Street, Rishikesh P Bhalerao, Zhaowei Yu, Yuhao Gao, Junbei Ni, Yang Jiao, Minghui Sun, Xiong Yang, Yixin Chen, Puyuan Liu, Jiaxi Wang, Yong Liu, Guolei Li
Hydrolyzable tannins (HTs), predominant polyphenols in oaks, are widely used in grape wine aging, feed additives, and human healthcare. However, the limited availability of a high-quality reference genome of oaks greatly hampered the recognition of the mechanism of HT biosynthesis. Here, high-quality reference genomes of three Asian oak species (Quercus variabilis, Quercus aliena, and Quercus dentata) that have different HT contents were generated. Multi-omics studies were carried out to identify key genes regulating HT biosynthesis...
March 21, 2024: New Phytologist
https://read.qxmd.com/read/38513474/a-dual-core-3d-dna-nanomachine-based-on-dnazyme-positive-feedback-loop-for-highly-sensitive-microrna-imaging-in-living-cells
#31
JOURNAL ARTICLE
Jinshen Li, Shixiu Xiao, Xin Wang, Xiaomei Mu, Shulin Zhao, Jianniao Tian
A double 3D DNA walker nanomachine by DNAzyme self-driven positive feedback loop amplification for the detection of miRNA was constructed. This method uses two gold nanoparticles as the reaction core, and because of the spatial confinement effect the local concentration of the reactants increase the collision efficiency was greatly improved. Meanwhile, the introduction of positive feedback loop promotes the conversion efficiency. In presence of miRNA-21, a large amount of DNAzyme was released and hydrolyze the reporter probe, resulting the recovery of fluorescence signal...
March 20, 2024: Talanta
https://read.qxmd.com/read/38513464/a-thermostable-xylanase-hydrolyzes-several-polysaccharides-from-bacillus-altitudinis-jyy-02-showing-promise-for-industrial-applications
#32
JOURNAL ARTICLE
Hongzheng Tai, Qunqun Guo, Jiamin Zhao, Yandong Liu, Hao Yu, Yili Liu, Yifan Qu, Guicai Du, Ronggui Li
Polysaccharides have attracted immense attention as the largest source of bioactive compounds. Its bioavailability and bioactivity can be improved by utilizing degradation enzymes to reduce their molecular weight and viscosity. In this study, a 654 bp gene encoding xylanase was screened from the genome of Bacillus altitudinis JYY-02 and overexpressed in Escherichia coli Rosetta (DE3). The recombinant xylanase with a molecular weight of 27.98 kDa was purified (11.7-fold) using Ni-NTA affinity chromatography, with a 43...
March 15, 2024: Carbohydrate Research
https://read.qxmd.com/read/38511880/modulation-of-phospholipase-a-2-membrane-activity-by-anti-inflammatory-drugs
#33
JOURNAL ARTICLE
Angela M Jaramillo-Granada, Jinhui Li, Aneliza Flores Villarreal, Omar Lozano, J C Ruiz-Suárez, Viviana Monje-Galvan, Francisco J Sierra-Valdez
The phospholipase A2 (PLA2 ) superfamily consists of lipolytic enzymes that hydrolyze specific cell membrane phospholipids and have long been considered a central hub of biosynthetic pathways, where their lipid metabolites exert a variety of physiological roles. A misregulated PLA2 activity is associated with mainly inflammatory-derived pathologies and thus has shown relevant therapeutic potential. Many natural and synthetic anti-inflammatory drugs (AIDs) have been proposed as direct modulators of PLA2 activity...
March 21, 2024: Langmuir: the ACS Journal of Surfaces and Colloids
https://read.qxmd.com/read/38508743/controlled-release-of-lysozyme-using-polyvinyl-alcohol-based-polymeric-nanofibers-generated-by-electrospinning
#34
JOURNAL ARTICLE
Riho Ogawa, Kouji Hara, Ayaka Kobayashi, Nobuyoshi Yoshimura, Yutaka Taniguchi, Eriko Yamazoe, Takaaki Ito, Kohei Tahara
Polymeric nanofibers generated via electrospinning offer a promising platform for drug delivery systems. This study examines the application of electrospun polyvinyl alcohol (PVA) nanofibers for controlled lysozyme (LZM) delivery. By using various PVA grades, such as the degree of polymerization/hydrolysis, this study investigates their influence on nanofiber morphology and drug-release characteristics. LZM-loaded PVA monolithic nanofibers having 50% drug content exhibit efficient entrapment, wherein rapid dissolution is achieved within 30 min...
2024: Chemical & Pharmaceutical Bulletin
https://read.qxmd.com/read/38508567/trends-in-the-development-and-current-perspective-of-thermostable-bacterial-hemicellulases-with-their-industrial-endeavors-a-review
#35
REVIEW
Fatima Akram, Taseer Fatima, Ramesha Ibrar, Ifrah Shabbir, Fatima Iftikhar Shah, Ikram Ul Haq
Hemicellulases are enzymes that hydrolyze hemicelluloses, common polysaccharides in nature. Thermophilic hemicellulases, derived from microbial strains, are extensively studied as natural biofuel sources due to the complex structure of hemicelluloses. Recent research aims to elucidate the catalytic principles, mechanisms and specificity of hemicellulases through investigations into their high-temperature stability and structural features, which have applications in biotechnology and industry. This review article targets to serve as a comprehensive resource, highlighting the significant progress in the field and emphasizing the vital role of thermophilic hemicellulases in eco-friendly catalysis...
March 18, 2024: International Journal of Biological Macromolecules
https://read.qxmd.com/read/38507999/evaluation-of-antioxidant-antidiabetic-and-antiobesity-potential-of-phenylpropanoids-pps-structure-activity-relationship-and-insight-into-action-mechanisms-against-dual-digestive-enzymes-by-comprehensive-technologies
#36
JOURNAL ARTICLE
Jiao Li, Chao-Feng Qin, Nai-Dong Chen
Phenylpropanoids (PPs), a group of natural compounds characterized by one or more C6-C3 units, have exhibited considerable potential in addressing metabolic disease. However, the comprehensive investigation on the relationship of compound structures and involved activity, along with the action mechanisms on the drug target is absent. This study aimed to evaluate the antioxidant and inhibitory activities of 16 PPs against two digestive enzymes, including α-glucosidase and pancreatic lipase, explore the structure-activity relationships and elucidate the mechanisms underlying enzyme inhibition...
March 16, 2024: Bioorganic Chemistry
https://read.qxmd.com/read/38507580/chimeric-nod2-mincle-agonists-as-vaccine-adjuvants
#37
JOURNAL ARTICLE
Emma M Dangerfield, Shigenari Ishizuka, Kristel Kodar, Sho Yamasaki, Mattie S M Timmer, Bridget L Stocker
There is a need for improved vaccine adjuvants to augment vaccine efficacy. One way to address this is by targeting multiple immune cell pathogen recognition receptors (PRRs) using chimeric pathogen-associated molecular patterns (PAMPs). Conjugation of the PAMPs will ensure codelivery of the immunostimulatory molecules to the same cell, enhancing adjuvant activity. The macrophage inducible C-type lectin (Mincle) is a promising PRR for adjuvant development; however, no effective chimeric Mincle adjuvants have been prepared...
March 20, 2024: Journal of Medicinal Chemistry
https://read.qxmd.com/read/38503170/structure-of-a-phosphodiesterase-from-streptomyces-sanglieri-with-a-novel-c-terminal-domain
#38
JOURNAL ARTICLE
Kazutaka Murayama, Toshiaki Hosaka, Mikako Shirouzu, Daisuke Sugimori
A glycerophosphoethanolamine ethanolaminephosphodiesterase (GPE-EP) from Streptomyces sanglieri hydrolyzes glycerophosphoethanolamine to phosphoethanolamine and glycerol. The structure of GPE-EP was determined by the molecular replacement method using a search model generated with AlphaFold2. This structure includes the entire length of the mature protein and it is composed of an N-terminal domain and a novel C-terminal domain connected to a flexible linker. The N-terminal domain is the catalytic domain containing calcium ions at the catalytic site...
March 13, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38502529/conditions-and-mechanism-of-formation-of-the-maillard-reaction-pigment-furpenthiazinate-in-a-model-system-and-in-some-acid-hydrolyzates-of-foods-and-its-biological-properties
#39
JOURNAL ARTICLE
Kyoko Noda, Marin Kishimoto, Yuko Shimamura, Masatsune Murata
Furpenthiazinate is a yellow pigment formed by the Maillard reaction between cysteine and furfural under strongly acidic conditions. Here, we describe the conditions and mechanism of pigment formation in a model system and in an acid hydrolyzate of food and analyze its biological properties. A reaction solution containing 32 mM cysteine and 128 mM furfural or 64 mM cysteine and 256 mM furfural in the presence of 2-6 M hydrochloric acid that was heated to 110 °C for 1-2 h yielded approximately 3 mM furpenthiazinate...
March 19, 2024: Journal of Agricultural and Food Chemistry
https://read.qxmd.com/read/38502458/molecular-cloning-and-functional-analysis-of-secretory-phospholipase-a-2-from-apostichopus-japonicus
#40
JOURNAL ARTICLE
Cheng Li, Lili Yang, Zhongyun Zhang, Ying Liu, Xu Li, Kai Yang, Ming Chen
Secretory phospholipase A2 (sPLA2 ) plays important roles in phospholipid metabolism, skin barrier maintenance, immune response and other processes in organisms. sPLA2 of sea cucumber A. japonicus (AjPLA2 ) has not yet been reported. This study successfully amplified the AjPLA2 sequence. The total cDNA of AjPLA2 is 931 bp, including a 480 bp ORF that encodes 159 amino acids. The AjPLA2 protein includes a 16-aa signal peptide, a 5-aa precursor peptide and a 138-aa mature peptide. Homologous alignment showed that AjPLA2 and the sPLA2 s from starfish have the typical domains of the Group IB sPLA2 ...
March 19, 2024: Biochemical Genetics
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