Julia Sánchez-Ceinos, Rocío Guzmán-Ruiz, Oriol Alberto Rangel-Zuñiga, Jaime López-Alcalá, Elena Moreno-Caño, Mercedes Del Río-Moreno, Juan Luis Romero-Cabrera, Pablo Pérez-Martínez, Elsa Maymo-Masip, Joan Vendrell, Sonia Fernández-Veledo, José Manuel Fernández-Real, Jurga Laurencikiene, Mikael Rydén, Antonio Membrives, Raul M Luque, José López-Miranda, María M Malagón
Preadipocytes are crucial for healthy adipose tissue expansion. Preadipocyte differentiation is altered in obese individuals, which has been proposed to contribute to obesity-associated metabolic disturbances. Here, we aimed at identifying the pathogenic processes underlying impaired adipocyte differentiation in obese individuals with insulin resistance (IR)/type 2 diabetes (T2D). We report that down-regulation of a key member of the major spliceosome, PRFP8 /PRP8, as observed in IR/T2D preadipocytes from subcutaneous (SC) fat, prevented adipogenesis by altering both the expression and splicing patterns of adipogenic transcription factors and lipid droplet-related proteins, while adipocyte differentiation was restored upon recovery of PRFP8 /PRP8 normal levels...
September 21, 2021: ELife