keyword
https://read.qxmd.com/read/38473722/immunological-landscapes-in-lung-transplantation-insights-from-t-cell-profiling-in-bal-and-pbmc
#1
JOURNAL ARTICLE
Tharushi Ayanthika de Silva, Simon Apte, Joanne Voisey, Kirsten Spann, Maxine Tan, Daniel Chambers, Brendan O'Sullivan
Lung transplant recipients frequently encounter immune-related complications, including chronic lung allograft dysfunction (CLAD). Monitoring immune cells within the lung microenvironment is pivotal for optimizing post-transplant outcomes. This study examined the proportion of T cell subsets in paired bronchoalveolar lavage (BAL) and peripheral PBMC comparing healthy ( n = 4) and lung transplantation patients ( n = 6, no CLAD and n = 14 CLAD) using 14-color flow cytometry. CD4+ T cell proportions were reduced in CD3 cells in both PBMC and BAL, and positive correlations were discerned between T cell populations in peripheral PBMC and BAL, suggesting the prospect of employing less invasive PBMC sampling as a means of monitoring lung T cells...
February 20, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38455264/optimization-of-anti-cd19-car-t%C3%A2-cell-production-for-treatment-of-patients-with-chronic-lymphocytic-leukemia
#2
JOURNAL ARTICLE
Christina Amatya, Katherine A Weissler, Vicki Fellowes, Norris Lam, Lauren C Cutmore, Danielle A Natrakul, Steven L Highfill, James N Kochenderfer
T cells expressing anti-CD19 chimeric antigen receptors (CARs) have activity against chronic lymphocytic leukemia (CLL), but complete response rates range from 18% to 29%, so improvement is needed. Peripheral blood mononuclear cells (PBMCs) of CLL patients often contain high levels of CLL cells that can interfere with CAR T cell production, and T cells from CLL patients are prone to exhaustion and other functional defects. We previously developed an anti-CD19 CAR designated Hu19-CD828Z. Hu19-CD828Z has a binding domain derived from a fully human antibody and a CD28 costimulatory domain...
March 14, 2024: Molecular Therapy. Methods & Clinical Development
https://read.qxmd.com/read/38441327/immune-modulation-by-nutritional-intervention-in-malnourished-children-identifying-the-phenotypic-distribution-and-functional-responses-of-peripheral-blood-mononuclear-cells
#3
JOURNAL ARTICLE
Zannatun Noor, Md Mehedi Hasan, Md Amran Gazi, Farzana Hossaini, Nur Muhammad Shahedul Haque, Parag Palit, Shah Mohammad Fahim, Subhasish Das, Mustafa Mahfuz, Chelsea Marie, William A Petri, Rashidul Haque, Tahmeed Ahmed
Malnourished children are susceptible to an increased risk of mortality owing to impaired immune functions. However, the underlying mechanism of altered immune functions and its interaction with malnutrition is poorly understood. This study investigates the immune function and evaluates the effect of a particular nutritional intervention on the immune cells of undernourished children. Stunted (LAZ <-2) and at-risk of being stunted (length-for-age Z-scores, LAZ <-1 to -2) children aged between 12 and 18 months were enrolled and were provided with the daily nutritional intervention of one egg and 150 mL cow's milk for 90 days...
September 2023: Scandinavian Journal of Immunology
https://read.qxmd.com/read/38422979/malt1-inhibition-suppresses-antigen-specific-t-cell-responses
#4
JOURNAL ARTICLE
Iliana K Kerzeli, Aikaterini Nasi, Erika Fletcher, Aikaterini Chourlia, Anders Kallin, Niklas Finnberg, Karolina Ersmark, Maria Lampinen, Mark Albertella, Fredrik Öberg, Sara M Mangsbo
The aim of this study was to assess the potential use of a selective small molecule MALT1 inhibitor in solid tumor treatment as an immunotherapy targeting regulatory T-cells (Tregs). In vitro, MALT1 inhibition suppressed the proteolytic cleavage of the MALT1-substrate HOIL1 and blocked IL-2 secretion in Jurkat cells. It selectively suppressed the proliferation of PBMC-derived Tregs, with no effect on conventional CD4+ T-cells. In vivo, however, no evident anti-tumor effect was achieved by MALT1 inhibition monotherapy or in combination with anti-CTLA4 in the MB49 cancer model...
February 24, 2024: Cellular Immunology
https://read.qxmd.com/read/38413280/neutralizing-antibody-titers-against-d8-genotype-and-persistence-of-measles-humoral-and-cell-mediated-immunity-eight-years-after-the-first-dose-of-measles-mumps-and-rubella-vaccine-in-brazilian-children
#5
JOURNAL ARTICLE
Letícia Kegele Lignani, Raquel de Vasconcellos Carvalhaes de Oliveira, Eliane Matos Dos Santos, Luiz Antonio Bastos Camacho, Janaína Reis Xavier, Gloria Regina da Silva E Sá, Marilda Mendonça Siqueira, Andréa Marques Vieira da Silva, Juliana Gil Melgaço, Nathalia Dos Santos Alves, Maria de Lourdes de Sousa Maia, Enirtes Caetano Prates Melo
OBJECTIVE: Assess the level of measles vaccine-induced neutralizing antibodies against the D8 genotype and the persistence of humoral and cell-mediated immunity in children who received their first dose of the measles, mumps, and rubella vaccine eight years previously. METHODS: Measles-specific IgG and neutralizing antibodies were determined in serum using ELISA and plaque reduction neutralization test, respectively. Cellular response was evaluated from peripheral blood mononuclear cells (PBMC)...
February 26, 2024: Vaccine
https://read.qxmd.com/read/38410517/cd4-th1-and-th17-responses-and-multifunctional-cd8-t-lymphocytes-associated-with-cure-or-disease-worsening-in-human-visceral-leishmaniasis
#6
JOURNAL ARTICLE
Mariana Nobre Farias de Franca, Lorranny Santana Rodrigues, Aline Silva Barreto, Geydson Silveira da Cruz, José Carlos Aragão-Santos, Angela Maria da Silva, Amélia Ribeiro de Jesus, Clarisa B Palatnik-de-Sousa, Roque Pacheco de Almeida, Cristiane Bani Corrêa
INTRODUCTION: In VL, a proinflammatory phenotype is typically associated with enhanced phagocytosis and a Th1 mediated immune response resulting in infection control. In contrast, an anti-inflammatory phenotype, associated with a predominant regulatory response, typically enables intracellular multiplication of Leishmania parasites and disease progression. METHODS: To investigate the impact of chemotherapy on Th2 and Th17 immune responses in patients with visceral leishmaniasis (VL), we assessed all combinations of intracellular expression of IFN-γ, IL-10, IL-4 and IL-17 in the CD4+ and CD8+ T cell populations of peripheral blood mononuclear cell (PBMC) samples from patients, after antigenic stimulation with Leishmania lysate, throughout treatment and follow-up...
2024: Frontiers in Immunology
https://read.qxmd.com/read/38384475/comparative-transcriptome-analysis-of-t-lymphocyte-subpopulations-and-identification-of-critical-regulators-defining-porcine-thymocyte-identity
#7
JOURNAL ARTICLE
Pingping Han, Wei Zhang, Daoyuan Wang, Yalan Wu, Xinyun Li, Shuhong Zhao, Mengjin Zhu
INTRODUCTION: The development and migration of T cells in the thymus and peripheral tissues are crucial for maintaining adaptive immunity in mammals. However, the regulatory mechanisms underlying T cell development and thymocyte identity formation in pigs remain largely underexplored. METHOD: Here, by integrating bulk and single-cell RNA-sequencing data, we investigated regulatory signatures of porcine thymus and lymph node T cells. RESULTS: The comparison of T cell subpopulations derived from porcine thymus and lymph nodes revealed that their transcriptomic differences were influenced more by tissue origin than by T cell phenotypes, and that lymph node cells exhibited greater transcriptional diversity than thymocytes...
2024: Frontiers in Immunology
https://read.qxmd.com/read/38370801/sars-cov-2-infection-elucidates-unique-features-of-pregnancy-specific-immunity
#8
Dong Sun Oh, Eunha Kim, Guangqing Lu, Rachelly Normand, Lydia L Shook, Amanda Lyall, Olyvia Jasset, Stepan Demidkin, Emily Gilbert, Joon Kim, Babatunde Akinwunmi, Jessica Tantivit, Alice Tirard, Benjamin Y Arnold, Kamil Slowikowski, Marcia B Goldberg, Michael R Filbin, Nir Hacohen, Long H Nguyen, Andrew T Chan, Xu G Yu, Jonathan Z Li, Lael Yonker, Alessio Fasano, Roy H Perlis, Ofer Pasternak, Kathryn J Gray, Gloria B Choi, David A Drew, Pritha Sen, Alexandra-Chloé Villani, Andrea G Edlow, Jun R Huh
Pregnancy is a risk factor for increased severity of SARS-CoV-2 and other respiratory infections. The mechanisms underlying this risk have not been well-established, partly due to a limited understanding of how pregnancy shapes immune responses. To gain insight into the role of pregnancy in modulating immune responses at steady state and upon perturbation, we collected peripheral blood mononuclear cells (PBMC), plasma, and stool from 226 women, including 152 pregnant individuals (n = 96 with SARS-CoV-2 infection and n = 56 healthy controls) and 74 non-pregnant women (n = 55 with SARS-CoV-2 and n = 19 healthy controls)...
February 7, 2024: medRxiv
https://read.qxmd.com/read/38369169/screening-for-immune-biomarkers-associated-with-infection-or-protection-against-ehrlichia-ruminantium-by-rna-sequencing-analysis
#9
JOURNAL ARTICLE
A Pretorius, T Nefefe, N Thema, J Liebenberg, H Steyn, M van Kleef
Heartwater is one of the most economically important tick-borne fatal diseases of livestock. The disease is caused by the bacteria Ehrlichia ruminantium transmitted by Amblyomma ticks. Although there is evidence that interferon-gamma controls E. ruminantium growth and that cellular immune responses are protective, an effective recombinant vaccine for this disease is lacking. Analyses of markers associated with infection as well as protection will lead to a better understanding of the E. ruminantium immune response and corresponding pathways induced in sheep peripheral blood mononuclear cells (PBMC) will assist in development of such a vaccine...
February 16, 2024: Microbial Pathogenesis
https://read.qxmd.com/read/38348038/radiofrequency-ablation-plays-double-role-in-immunosuppression-and-activation-of-pbmcs-in-recurrent-hepatocellular-carcinoma
#10
JOURNAL ARTICLE
Yang Zhao, Tongwang Yang, Yabo Ouyang, Wei Rao, Kai Liu, Jiasheng Zheng, Fudong Lv, Ying Shi, Feng Wang, Dongjie Liu, Luxin Qiao, Zhenying Xia, Yushi Zhang, Dexi Chen, Wenjing Wang
BACKGROUND: Radiofrequency ablation (RFA) is the primary curative treatment for hepatocellular carcinoma (HCC) patients who are not eligible for surgery. However, the effects of RFA on the global tumor immune response remain unclear. METHOD: In this study, we examined the phenotypic and functional changes in peripheral blood mononuclear cells (PBMCs) from recurrent HCC patients who had undergone two RFA treatments using mass cytometry and high-throughput mRNA assays...
2024: Frontiers in Immunology
https://read.qxmd.com/read/38279483/immunomodulatory-effect-and-molecular-mechanism-of-deoxyribonucleic-acid-receptor-toll-like-receptor-9-signaling-pathway-on-newborn-babies-with-acute-lung-injury
#11
JOURNAL ARTICLE
Lei Dou, Yannan Chai, Huiju Shi
This work was to demonstrate the immunomodulatory effect of toll-like receptor 9 (TLR9) signaling on newborn babies with acute lung injury (ALI) and the mechanism of TLR9 in vivo, so as to provide experimental basis for clinical treatment of newborn babies with ALI. Firstly, the expression of TLR9 in peripheral blood mononuclear cell (PBMC) was compared among ALI and healthy newborn babies. Then, PBMCs of newborn babies with ALI were extracted and divided into three groups. They were added with non-methylated cytosine purine-guanine dinucleotide sequence oligodeoxyribonucleotide (CpG ODN), ODN without non-methylated CpG, and blank nutrient solution, respectively, so as to determine the proliferation changes of PBMC...
December 31, 2023: Cellular and Molecular Biology
https://read.qxmd.com/read/38272735/zika-virus-precursor-membrane-peptides-induce-immune-response-in-peripheral-blood-mononuclear-cells
#12
JOURNAL ARTICLE
Yogita Gupta, Manoj Baranwal, Bhupendra Chudasama
Zika virus is a re-merging flavivirus allied to serious mental health conditions in the fetuses. There is currently no preventives or treatment available for Zika infection. In this work, we have extended the in silico analysis by performing the molecular docking of previous reported three conserved Zika virus precursor membrane (prM) peptides (MP1, MP2 and MP3) with HLA complex (pHLA) and T cell receptors (TCR) and also evaluated the peptide specific immune response in human peripheral blood mononuclear cells (PBMC)...
January 24, 2024: Human Immunology
https://read.qxmd.com/read/38253675/non-fatal-outcomes-of-covid-19-disease-in-pediatric-organ-transplantation-associates-with-down-regulation-of-senescence-pathways
#13
JOURNAL ARTICLE
Kumar Subramanian, Rency Varghese, Molly Pochedly, Vinona Muralidaran, Nada Yazigi, Stuart Kaufman, Khalid Khan, Bernadette Vitola, Alexander Kroemer, Thomas Fishbein, Habtom Ressom, Udeme D Ekong
This is a cross-sectional study examining kinetics and durability of immune response in children with solid organ transplants (SOTs) who had COVID-19 disease between November 2020 through June 2022, who were followed for 60-days at a single transplant center. Blood was collected between 1-14 (acute infection), and 15-60 days of a positive PCR (convalescence). SOT children with peripheral blood mononuclear cells (PBMC) cryopreserved before 2019 were non-infected controls (ctrls). PBMCs stimulated with 15-mer peptides from spike protein and anti-CD49d/anti-CD28...
January 22, 2024: Scientific Reports
https://read.qxmd.com/read/38250827/assessment-of-human-sars-cov-2-specific-t-cell-responses-elicited-in-vitro-by-new-computationally-designed-mrna-immunogens-covarna
#14
JOURNAL ARTICLE
Ignasi Esteban, Carmen Pastor-Quiñones, Lorena Usero, Elena Aurrecoechea, Lorenzo Franceschini, Arthur Esprit, Josep Lluís Gelpí, Francisco Martínez-Jiménez, Núria López-Bigas, Karine Breckpot, Kris Thielemans, Lorna Leal, Carmen Elena Gómez, Marta Sisteré-Oró, Andreas Meyerhans, Mariano Esteban, María José Alonso, Felipe García, Montserrat Plana
The COVID-19 pandemic has brought significant changes and advances in the field of vaccination, including the implementation and widespread use of encapsidated mRNA vaccines in general healthcare practice. Here, we present two new mRNAs expressing antigenic parts of the SARS-CoV-2 spike protein and provide data supporting their functionality. The first mRNA, called RBD-mRNA, encodes a trimeric form of the virus spike protein receptor binding domain (RBD). The other mRNA, termed T-mRNA, codes for the relevant HLA I and II spike epitopes...
December 22, 2023: Vaccines
https://read.qxmd.com/read/38167452/a-novel-pdpn-antagonist-peptide-cy12-rp2-inhibits-melanoma-growth-via-wnt-%C3%AE-catenin-and-modulates-the-immune-cells
#15
JOURNAL ARTICLE
Chunyan Feng, Albert Yu, Zhongfu Wang, Kun Wang, Jiawei Chen, Yaojiong Wu, Ting Deng, Huaqing Chen, Yibo Hou, Shaohua Ma, Xiaoyong Dai, Laiqiang Huang
BACKGROUND: Podoplanin (PDPN) is a highly conserved, mucin-type protein specific to the lymphatic system. Overexpression of PDPN is associated with the progression of various solid tumors, and plays an important roles in the tumor microenvironment by regulating the immune system. However, the role of PDPN-mediated signal activation in the progression of melanoma is still unknown. METHODS: PDPN expression was first analyzed in 112 human melanoma tissue microarrays and melanoma cell lines...
January 2, 2024: Journal of Experimental & Clinical Cancer Research: CR
https://read.qxmd.com/read/38161282/upregulation-of-probdnf-p75ntr-signaling-in-immune-cells-and-its-correlation-with-inflammatory-markers-in-patients-with-major-depression
#16
JOURNAL ARTICLE
Chun-Rui Yang, Rui Liang, Yuan Liu, Fan-Jie Meng, Fiona Zhou, Xiao-Yang Zhang, Li Ning, Zhi-Qiang Wang, Shuang Liu, Xin-Fu Zhou
ProBDNF is the precursor protein of brain-derived neurotrophic factor (BDNF) expressed in the central nervous system and peripheral tissues. Previous studies showed that the blood levels of both proBDNF and p75 neurotrophic receptors (p75NTR) in major depressive disorder (MDD) were increased, but which blood cell types express proBDNF and its receptors is not known. Furthermore, the relationship between proBDNF/p75NTR and inflammatory cytokines in peripheral blood of MDD is unclear. Peripheral blood mononuclear cells (PBMCs) and serum were obtained from depressive patients (n = 32) and normal donors (n = 20)...
January 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38141722/tcrseq-reveals-selected-donor-reactive-cd8-t-cell-clones-resist-anti-thymocyte-globulin-depletion-after-kidney-transplantation
#17
JOURNAL ARTICLE
Mehek Ningoo, Pamela Cruz-Encarnación, Calla Khilnani, Peter S Heeger, Miguel Fribourg
High frequencies of donor-reactive memory T cells in the periphery of transplant candidates prior to transplantation are linked to the development of post-transplant acute rejection episodes and to reduced allograft function. Rabbit anti-thymocyte globulin (rATG) effectively depletes naïve CD4+ and CD8+ T cells for > 6 months post-transplant, but rATG's effects on human donor-reactive T cells have not been carefully determined. To address this, we performed T cell receptor beta chain sequencing (TCRseq) on PBMC aliquots collected pre-transplant and serially post-transplant in seven kidney transplant recipients who received rATG as induction therapy...
December 21, 2023: American Journal of Transplantation
https://read.qxmd.com/read/38124744/immunogenicity-of-pe18-pe31-and-ppe26-proteins-from-mycobacterium-tuberculosis-in-humans-and-mice
#18
JOURNAL ARTICLE
María García-Bengoa, Emil Joseph Vergara, Andy C Tran, Lorenzo Bossi, Andrea M Cooper, John E Pearl, Tufária Mussá, Maren von Köckritz-Blickwede, Mahavir Singh, Rajko Reljic
INTRODUCTION: The large family of PE and PPE proteins accounts for as much as 10% of the genome of Mycobacterium tuberculosis . In this study, we explored the immunogenicity of three proteins from this family, PE18, PE31, and PPE26, in humans and mice. METHODS: The investigation involved analyzing the immunoreactivity of the selected proteins using sera from TB patients, IGRA-positive household contacts, and IGRA-negative BCG vaccinated healthy donors from the TB endemic country Mozambique...
2023: Frontiers in Immunology
https://read.qxmd.com/read/38111075/in-vivo-study-of-newly-developed-albumin-conjugated-urate-oxidase-for-gout-treatment
#19
JOURNAL ARTICLE
Jeonghaeng Cho, Byungseop Yang, Jae Hun Lee, Hyunwoo Kim, Hyeongseok Kim, Eun Byeol Go, Dong-Ho Bak, Su Jin Park, Inchan Kwon, Jong-Il Choi, Kyunghee Lee
BACKGROUND: Exogenously providing engineered Uox with enhanced half-life is one of the important urate-lowering treatments for gout. The potential of PAT101, a recombinant human albumin (rHA)-conjugated variant, was evaluated and compared as a novel gout treatment through various in vivo studies with PAT101 and competing drugs. METHODS: PAT101 was produced by site-specific conjugation of rHA and Aspergillus flavus Uox (AfUox-rHA) through clickable non-natural amino acid (frTet) and Inverse electron demand Diels-Alder (IEDDA) reaction...
December 18, 2023: Arthritis Research & Therapy
https://read.qxmd.com/read/38097459/identification-of-t-cell-and-linear-b-cell-epitopes-on-african-horse-sickness-virus-serotype-4-proteins-vp1-1-vp2-vp4-vp7-and-ns3
#20
JOURNAL ARTICLE
Erika Faber, Antoinette van Schalkwyk, Selaelo Ivy Tshilwane, Mirinda Van Kleef, Alri Pretorius
The viral proteins VP1-1, VP2, VP4, VP7 and NS3, of African horse sickness virus serotype 4 (AHSV4), have previously been identified to contain CD8+ T cell epitopes. In this study, overlapping peptides spanning the entire sequences of these AHSV4 proteins were synthesized and used to map epitopes. Peripheral blood mononuclear cells (PBMC) isolated from five horses immunized with an attenuated AHSV4 were stimulated in vitro with the synthesized peptides. Various memory immune assays were used to identify the individual peptides that contain CD8+ T cell epitopes, CD4+ T cell epitopes and linear B cell epitopes...
December 13, 2023: Vaccine
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