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Sukhwinder Singh Sohal, Mathew Suji Eapen, Vegi G M Naidu, Pawan Sharma
Heat-not-burn (HNB) devices can alter vital physiological functions in the lung. HNB devices may not be a safer option than cigarette smoking or eCig vaping; this does not support the recommendation of their use over other nicotine delivery products.
February 2019: ERJ Open Research
Cinzia De Marco, Alessandro Borgini, Ario Alberto Ruprecht, Chiara Veronese, Roberto Mazza, Martina Bertoldi, Andrea Tittarelli, Alessandra Scaburri, Anna Chiara Ogliari, Vincenzo Zagà, Paolo Contiero, Giovanna Tagliabue, Roberto Boffi
The spread of electronic cigarettes (e-cigs) and of the so-called heat-not-burn (HnB), also known as heated tobacco products, presented as a less harmful alternative to traditional cigarettes, required further in-depth studies to demonstrate the real benefits or possible risks linked to this type of habit among smokers and possible new smokers. There are numerous harmful substances produced by these devices, such as metals, organic compounds, and aldehydes. The presence of formaldehyde is particularly worrying: its indoor concentration is 2...
September 2018: Epidemiologia e Prevenzione
Prabhu Narayan Meena, Lalit Laxman Kharbikar, Rajeev Singh Rana, Subrata Satpathy, Arti Shanware, Palaiyur Nanjappan Sivalingam, Shweta Nandanwar
A loop-mediated isothermal amplification (LAMP) assay was optimized for the detection of Mesta yellow vein mosaic virus (MeYVMV) in diseased plants of mesta (Hibiscus sabdariffa L.& H. cannabinus L.). The LAMP assay was optimized using a set of six primers targeting the MeYVMV genome and could be completed in 30-60 min at 63 °C. The LAMP amplification results were visualized by adding 1 μl of hydroxy naphthol blue (HNB) dye in a 25 μl LAMP reaction mixture prior to amplification as well as by electrophoresis...
October 22, 2018: Journal of Virological Methods
Helena Sprafke, Rupert Palme, Paul Schmidt, Michael Erhard, Shana Bergmann
The welfare of two genetic lines of organic layer hen pullets-H&N Super Nick (HNS) and H&N Brown Nick (HNB)-was compared during two commercial transport variants of 15 flocks of mixed-reared birds. Birds were either transported overnight (with a break in travel), or were transported direct to the layer farm (without a break in travel). Samples of feces were collected non-invasively from 25 birds of each genetic line per flock for each transport variant before transportation to evaluate baseline values of glucocorticoid metabolites, and at 0 h, 3 h, 6 h, 10 h, 24 h, 34 h, 48 h, 58 h, and 72 h after the end of transportation, to measure transportation and translocation stress...
October 19, 2018: Animals: An Open Access Journal From MDPI
Daniel Kotz, Sabrina Kastaun
BACKGROUND: Electronic cigarettes (ECs) and heat-not-burn (HNB) devices are relatively new products that are increasingly used alternatively or complementary to ordinary tobacco products. OBJECTIVES: To analyse current data from the German Study on Tobacco Use (DEBRA) regarding the prevalence of EC and HNB device use and associated socioeconomic factors and smoking behaviour; compare reasons for EC use between adolescents and adults; describe the self-perceived risk of HNB devices...
November 2018: Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz
Elke Pieper, Nadja Mallock, Frank Henkler-Stephani, Andreas Luch
Increased tobacco control measures in recent years have directed the tobacco industry to develop alternative tobacco products, such as "heat not burn" (HnB) tobacco devices that are implied to be less hazardous than conventional cigarettes. There are extensive studies from manufacturers available, which show that the emissions of HnB tobacco devices have significantly lower levels of harmful substances compared to conventional cigarettes. In addition, manufacturers have published studies to investigate whether switching from a conventional tobacco cigarette to the HnB product reduces possible health risks...
November 2018: Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz
Satoru Munakata, Kanae Ishimori, Nobumasa Kitamura, Shinkichi Ishikawa, Yuichiro Takanami, Shigeaki Ito
The use of novel tobacco- and nicotine-containing vapor products that do not combust tobacco leaves is on the rise worldwide. The emissions of these products typically contain lower numbers and levels of potentially harmful chemicals compared with conventional cigarette smoke. These vapor products may therefore elicit fewer adverse biological effects. We compared the effects of emissions from different types of such products, i.e., our proprietary novel tobacco vapor product (NTV), a commercially available heat-not-burn tobacco product (HnB), and e-cigarette (E-CIG), and a combustible cigarette in a human bronchial epithelial cell line...
November 2018: Regulatory Toxicology and Pharmacology: RTP
Erikas Simonavicius, Ann McNeill, Lion Shahab, Leonie S Brose
OBJECTIVE: To review peer-reviewed evidence on heat-not-burn tobacco products (HnB), their secondhand emissions and use by humans; to identify differences between independent and industry-funded studies. DATA SOURCES: Medline, Embase, PsycINFO, ProQuest, Scopus and Web of Science databases were searched up to 6 November 2017 for studies on HnB published after December 2009; reference lists were screened and other researchers contacted, yielding 637 records. STUDY SELECTION: Thirty-one publications on HnB secondhand emissions (n=16) or use by humans (n=15) were selected by two reviewers with excellent agreement (k=0...
September 4, 2018: Tobacco Control
Vyas Kumar Rathaur, Monika Pathania, Charu Pannu, Anand Jain, Minakshi Dhar, Nitish Pathania, Rahul Goel
Introduction: There is paucity of studies on infant feeding practices from the rural areas of garhwal Himalayas of the state of uttarakhand. The present study was designed to assess the infant feeding practices in Garhwal region. Infant feeding practices have significant implications on a child's health. Early nutritional status especially during the first year of life has been shown to have a significant effect on child health and development. Optimal infant feeding practices are crucial for nutritional status, growth, development, health, and ultimately the survival of infants and young children...
January 2018: Journal of Family Medicine and Primary Care
Nuket Kartal Temel, Kadriye Sertakan, Ramazan Gürkan
In this study, ultrasound-assisted cloud point extraction (UA-CPE) method was developed for the determination of Ni(II) and Co(II) in milk-based products. After extraction and preconcentration, the Ni(II) and Co(II) contents of samples were determined by flame atomic absorption spectrometry (FAAS). After their complexation with hydroxy naphthol blue (HNB) in the presence of cationic surfactant, CTAB at pH 4.0, the Ni(II) and Co(II) were taken within the micellar phase of nonionic surfactant, TX-114. The micellar phase containing the analytes were diluted to a volume of 0...
December 2018: Biological Trace Element Research
Madhvi Chahar, Anup Anvikar, Rajnikant Dixit, Neena Valecha
Loop mediated isothermal amplification (LAMP) assay is sensitive, prompt, high throughput and field deployable technique for nucleic acid amplification under isothermal conditions. In this study, we have developed and optimized four different visualization methods of loop-mediated isothermal amplification (LAMP) assay to detect Pfcrt K76T mutants of P. falciparum and compared their important features for one-pot in-field applications. Even though all the four tested LAMP methods could successfully detect K76T mutants of P...
July 2018: Experimental Parasitology
C Manjunatha, Sapna Sharma, Deepika Kulshreshtha, Sangeeta Gupta, Kartar Singh, Subhash C Bhardwaj, Rashmi Aggarwal
Leaf rust of wheat caused by Puccinia triticina has significant impact on wheat production worldwide. Effective and quick detection methodologies are required to mitigate yield loss and time constraints associated with monitoring and management of leaf rust of wheat. In the present study, detection of P. triticina has been simplified by developing a rapid, reliable, efficient and visual colorimetric method i.e., loop mediated isothermal amplification of DNA (LAMP). Based on in silico analysis of P. triticina genome, PTS68, a simple sequence repeat was found highly specific to leaf rust fungus...
2018: PloS One
Q Zhong, K Li, D Chen, H Wang, Q Lin, W Liu
Objective Condyloma acuminatum (CA) is a common, viral, sexually transmitted disease worldwide. Human papillomavirus (HPV) genotyping has important clinical implications for the treatment of CA. We developed a loop-mediated isothermal amplification (LAMP) method for the detection of HPV. Methods We collected 294 cervical scrape samples, including 30 HPV-6-positive, 30 HPV-11-positive, 22 HPV-16-positive, 20 HPV-42-positve, 30 HPV-43-positive, 20 HPV-44-positive and 142 HPV-negative samples. Tissues from 40 patients with a pathological diagnosis of CA were paraffin-embedded and analyzed by LAMP and Luminex...
July 2018: British Journal of Biomedical Science
Xiangyu Li, Yanbo Luo, Xingyi Jiang, Hongfei Zhang, Fengpeng Zhu, Shaodong Hu, Hongwei Hou, Qingyuan Hu, Yongqiang Pang
Introduction: Tobacco Heating System 2.2 (THS 2.2, marketed as iQOS) is a heat-not-burn (HNB) tobacco product that has been successfully introduced to global markets. Despite its expanding market, few independent and systematic researches into THS 2.2 have been carried out to date. Methods: We tested a comprehensive list of total particulate matter (TPM), water, tar, nicotine, propylene glycol, glycerin, carbon monoxide, volatile organic compounds, aromatic amines, hydrogen cyanide, ammonia, N-nitrosamines, phenol, and polycyclic aromatic hydrocarbon under both ISO and HCI regimes...
January 1, 2019: Nicotine & Tobacco Research: Official Journal of the Society for Research on Nicotine and Tobacco
Vinoth Ganesan, Sunghyun Lim, Jinkwon Kim
The development of hydrogen evolution catalysts based on nonprecious metals is essential for the practical application of water-splitting devices. Herein, the synthesis of Co9 S8 -MoS2 hierarchical nanoboxes (HNBs) as efficient catalysts for the hydrogen evolution reaction (HER) is reported. The surface of the hollow cubic structure was organized by CoMoS4 nanosheets formed through the reaction of MoS4 2- and Co2+ released from the cobalt zeolite imidazole framework (ZIF-67) templates under reflux in a mixture of water/ethanol...
February 16, 2018: Chemistry, An Asian Journal
Takahiro Tabuchi, Silvano Gallus, Tomohiro Shinozaki, Tomoki Nakaya, Naoki Kunugita, Brian Colwell
OBJECTIVES: A heat-not-burn (HNB) tobacco product, IQOS, was first launched in Japan and Italy as test markets and is currently in commerce in 30 countries. Using two data sources, we examined interest in HNB tobacco (IQOS, Ploom and glo), its prevalence, predictors of its use and symptoms from exposure to secondhand HNB tobacco aerosol in Japan, where HNB tobacco has been sold since 2014. METHODS: Population interest in HNB tobacco was explored using Google search query data...
July 2018: Tobacco Control
Diego Paim Carvalho Garcia, Clarissa Santos, Cristiana Buzelin Nunes, Marcelo Araújo Buzelin, Andy Petroianu, Luiza Ohasi de Figueiredo, Andreia Souto da Motta, Cristiane de Barros Gaspar, Luiz Ronaldo Alberti
PURPOSE: To compare the effectiveness of light-weight polypropylene mesh coated with polymerized and purified bovine type I collagen (Surgidry HNB) in the treatment of abdominal wall defect and the degree of adhesion formation. METHODS: Two types of polypropylene mesh were implanted after creation of defect measuring 6.0cm X 5.5cm in the anterior abdominal wall of 32 male New Zealand breed rabbits, divided in two groups (n = 32): (1) light-weigh macroporous polypropylene, (2) type I polymerized and purified bovine collagen coated light-weigh macroporous polypropylene...
November 2017: Acta Cirúrgica Brasileira
Caroline Ajonina, Christopher Buzie, Julia Möller, Ralf Otterpohl
To increase current knowledge on the epidemiology of protozoan parasites in wastewater treatment plants (WWTP), the occurrence of Entamoeba histolytica and Toxoplasma gondii in raw and treated wastewater was investigated. Samples were collected from WWTP twice a month over a period of 8 months. Determination of protozoa was performed using polymerase chain reaction (PCR) and light microscopy. After concentration and purification of wastewater samples, DNA extraction was conducted followed by PCR amplification of small subunit ribosomal RNA (SSU rRNA) gene sequences of E...
2018: Journal of Toxicology and Environmental Health. Part A
Dongxin Liu, Bing Zhao, Xichao Ou, Huiwen Zheng, Aijing Ma, Wencong He, Shengfen Wang, Yang Zhou, Hui Xia, Yang Zheng, Ping Hou, Guangxue He, Yanlin Zhao
Mycobacterium tuberculosis (MTB) is the causative agent of pulmonary tuberculosis. Rapid and accurate diagnosis is crucial to tuberculosis control and prevention. A series of diagnostic methods has been available for MTB detection; however, new rapid, simple and affordable methods are needed. In this study, a multiple cross displacement amplification (MCDA)-based assay was developed to detect the IS6110 gene of the M. tuberculosis complex. Hydroxy naphthol blue (HNB), a colorimetric indicator, was used to detect amplification products...
February 2018: Journal of Microbiological Methods
X R Hu, D J Dai, H D Wang, C Q Zhang
Botrytis cinerea, a typical "high-risk" pathogenic fungus that rapidly develops resistance to fungicides, affects more than 1,000 species of 586 plant genera native to most continents and causes great economic losses. Therefore, a rapid and sensitive assay of fungicide resistance development in B. cinerea populations is crucial for scientific management. In this study, we established a Loop-mediated isothermal amplification (LAMP) system for the monitoring and evaluation of the risk of development of B...
October 24, 2017: Scientific Reports
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