keyword
https://read.qxmd.com/read/38184269/sperm-plasma-membrane-ion-transporters-and-male-fertility-potential-a-perspective-under-the-prism-of-cryopreservation
#21
REVIEW
Sevastiani Antonouli, Valentina Di Nisio, Christina Messini, Maria Samara, Andres Salumets, Alexandros Daponte, George Anifandis
Intracellular calcium homeostasis plays a crucial role in spermatozoa by regulating physiological functions associated with sperm quality and male fertility potential. Intracellular calcium fine balance in the sperm cytoplasm is strictly dependent on sperm surface channels including the CatSper channel. CatSpers' role is to ensure the influx of extracellular calcium, while intracellular pH alkalinization serves as a stimulus for the activation of several channels, including CatSper. Overall, the generation of intracellular calcium spikes through CatSper is essential for fertilization-related processes, such as sperm hyperactivation, acrosome reaction, egg chemotaxis, and zona pellucida penetration...
January 4, 2024: Cryobiology
https://read.qxmd.com/read/38163687/high-temperature-glycosylation-of-saccharides-to-modify-molecular-conformation-of-egg-white-protein-and-its-effect-on-the-stability-of-high-internal-phase-emulsions
#22
JOURNAL ARTICLE
Fei Lu, Yujie Chi, Yuan Chi
This paper investigates the freeze-thaw stability of oil-in-water emulsions stabilized by high-temperature wet heating glycosylation products. Glucose (Glu), D-fructose (Fru), xylose (Xyl), maltodextrin (MD), oligofructose (FO), and oligomeric isomaltulose (IMO) were chosen as sugar sources for the glycosylation reaction with egg white proteins (EWPs) at 120 °C to prepare the GEWPs. The study reveals that the type of sugar significantly influences the Maillard reactions with EWPs. The degree of glycosylation was highest in the Xyl group with the greatest reducing capacity and lowest in the MD, FO, and IMO groups...
January 2024: Food Research International
https://read.qxmd.com/read/38136998/comparative-transcriptome-analysis-of-galeruca-daurica-reveals-cold-tolerance-mechanisms
#23
JOURNAL ARTICLE
Hongling Zhang, Feilong Sun, Wenbing Zhang, Xia Gao, Lei Du, Xiaopeng Yun, Yanyan Li, Ling Li, Baoping Pang, Yao Tan
Galeruca daurica (Joannis) is a pest species with serious outbreaks in the Inner Mongolian grasslands in recent years, and its larvae and eggs are extremely cold-tolerant. To gain a deeper understanding of the molecular mechanism of its cold-tolerant stress response, we performed de novo transcriptome assembly of G. daurica via RNA-Seq and compared the differentially expressed genes (DEGs) of first- and second-instar larvae grown and developed indoors and outdoors, respectively. The results show that cold tolerance in G...
December 4, 2023: Genes
https://read.qxmd.com/read/38127680/amoxicillin-loaded-bionanocomposite-hydrogels-swelling-dehydration-and-in-vitro-drug-release-kinetics-and-mechanism
#24
JOURNAL ARTICLE
Mona Ghasemzadeh, Sahel Gozalzadeh, Mohammad Sirousazar, Farshad Kheiri
This study deals with preparing and characterizing polyvinyl alcohol/egg white/montmorillonite bionanocomposite hydrogels as antibacterial drug delivery systems. The cyclic freezing/thawing method was utilized to fabricate the hydrogels. To study the performance of the prepared hydrogels as drug delivery systems, amoxicillin, as a model antibiotic drug, was loaded into the hydrogels by mixing with the precursor polymer solution and gelation. From the diverse microstructural characterization techniques, i.e...
December 21, 2023: Journal of Biomaterials Science. Polymer Edition
https://read.qxmd.com/read/38082221/efficient-pathway-for-men-fertility-preservation-in-testicular-cancer-or-lymphoma-a-cross-sectional-study-of-national-2018-data
#25
JOURNAL ARTICLE
Ségolène Prades, Sarah-Lyne Jos, Jacqueline Saïas-Magnan, Louis Bujan, Florence Eustache, Oxana Blagosklonov, Eric Lechevallier, Florence Brugnon, Vanessa Loup-Cabaniols, Dorian Bosquet, Marie Prades, Bérengère Ducrocq, Céline Chalas, Sandrine Giscard-d'Estaing, Anne Mayeur, Isabelle Koscinsky, Françoise Schmitt, Aline Papaxanthos-Roche, Marius Teletin, Emmanuelle Thibault, Damien Beauvillard, Sophie Mirallie, Béatrice Delepine, Annie Benhaim, Pascale May-Panloup, Ségolène Veau, Cynthia Frapsauce, Patricia Fauque, Régis Costello, Nathalie Rives, Catherine Metzler-Guillemain, Jeanne Perrin
BACKGROUND: In 15-49 years-old men, the main cancers are testicular cancer (TC) and lymphomas (L): freezing of ejaculated sperm is primarily used for male fertility preservation (FP) before cancer treatment. Our objective was to analyze the French FP rate in 15-49 years-old men diagnosed with TC or L in 2018. We designed a national descriptive cross-sectional study of sperm banking rate in men with a diagnosis of TC, Hodgkin L (HL) or non-Hodgkin L (NHL). From the French National Cancer Institute (INCa) 2018 data, we extracted the estimated incidence of TC and L in metropolitan France...
December 12, 2023: Basic and Clinical Andrology
https://read.qxmd.com/read/38038214/reduction-of-cryopreservation-induced-structural-functional-and-molecular-damages-in-ram-sperm-by-hydrated-c-60-fullerene
#26
JOURNAL ARTICLE
İbrahim Halil Güngör, Gaffari Türk, Serap Dayan Cinkara, Tutku Can Acisu, Ahmet Tektemur, Deniz Yeni, Fatih Avdatek, Gözde Arkali, Recep Hakkı Koca, Şeyma Özer Kaya, Meltem Sagiroglu, Ebru Etem Önalan, Mustafa Sönmez, Seyfettin Gür, Abdurrauf Yüce
This study aimed to investigate the morphological, functional and molecular changes in frozen-thawed ram sperm using an extender containing different concentrations of hydrated carbon 60 fullerene (C60 HyFn), a nanotechnological product. Semen taken from each of the seven Akkaraman rams were pooled. Semen collection was done twice a week and it continued for 3 weeks. Each pooled semen sample was divided into six equal groups and diluted with tris + egg yolk extender including 0 (control), 200, 400, 800 nM, 1 and 5 μM concentrations of C60 HyFn at 37°C...
December 1, 2023: Reproduction in Domestic Animals
https://read.qxmd.com/read/38034211/exploring-the-landscape-of-social-egg-freezing-navigating-medical-advancements-ethical-dilemmas-and-societal-impacts
#27
REVIEW
Udit Lahoti, Sandhya Pajai, Tejas Shegekar, Anup Juganavar
This narrative review article comprehensively explores the multifaceted landscape of social egg freezing, delving into its medical, ethical, societal, psychological, legal, and cultural dimensions. Oocyte cryopreservation, a developing procedure, gives women the chance to match their life goals with fertility goals. Informed decision-making, morally sound guidance, and open communication are all stressed by ethical considerations. Family planning practices, workplace cultures, and gender equality all have an impact on societal dynamics...
October 2023: Curēus
https://read.qxmd.com/read/38032310/effect-of-membrane-stabilizers-on-semen-quality-and-sperm-membrane-protein-expression-during-cryopreservation-of-goat-semen
#28
JOURNAL ARTICLE
M Dutta, G Kadirvel, P Borah, S Sinha, K Ahmed, G Hazarika, R Sharma, H Choudhury, S Deori, M Das Gupta, R K Biswas, S Tamuly, P M Barua, J Hussain
BACKGROUND: Semen cryopreservation is a complex process during which there is alteration in the expression of sperm and seminal plasma proteins, molecular weight of protein or loss of membrane proteins during the process. In order to compensate for these changes, different membrane stabilizers are used in freezing semen extenders. However, there is scarcity of such studies during cryopreservation of goat semen. OBJECTIVE: To investigate the effect of membrane stabilizers on sperm membrane protein expression during cryopreservation of goat semen...
2023: Cryo Letters
https://read.qxmd.com/read/38025996/use-of-alternative-diluent-acp-lact%C3%A2-and-coconut-water-powder-acp-in-cryopreservation-of-goat-semen-and-fixed-time-artificial-insemination-ftai-in-half-breed-goats
#29
JOURNAL ARTICLE
Leonardo Alves Rodrigues Cabral, Wallisson Bruno de Morais Pacheco, Suane Silva Alves Dos Santos, Adriano da Silva Prado, Luis Alberto Linhares Rufino, Cristiane Clemente de Mello Salgueiro, Ney Rômulo de Oliveira Paula, Assis Rubens Montenegro, José Ferreira Nunes
The aim of the present study was to evaluate semen cryopreservation with ACP-Lact® diluent, which consists of coconut water powder (ACP) added to goat milk powder. After thawing, the samples were evaluated for sperm kinetics, membrane evaluation and in vivo insemination. For cryopreservation, a pool was made with the ejaculate of six goats, diluted in four equal aliquots for the respective treatments: T1 (ACP-Lact®); T2 (ACP-Lact® 50%); T3 (ACP + 2.5% egg yolk) and T4 (Tris + 2.5% egg yolk). After dilution of the treatments, the samples were placed in 0...
2023: Animal Reproduction
https://read.qxmd.com/read/38024487/social-egg-freezing-for-single-women-in-china-legal-and-ethical-controversies
#30
JOURNAL ARTICLE
Yue Zhao, Huixian Fu
Social egg freezing is an emerging topic in China, where single women currently face restrictions in accessing this service at medical facilities. Administrative regulations prohibit single women from accessing all forms of assisted reproductive technologies. Both the first lawsuit filed by a single woman seeking to freeze her eggs and a legislative proposal aiming to protect single women's rights to access assisted reproductive technologies were denied. This has sparked controversy and discussion about the reproductive rights of single women and the legitimacy of their access to medical and technical assistance...
2023: Risk Management and Healthcare Policy
https://read.qxmd.com/read/38018440/influence-of-nano-formulations-of-clove-bud-syzygium-aromaticum-extract-on-freezing-ability-antioxidant-capacity-caspase-3-activity-acrosome-reaction-and-fertility-of-frozen-rabbit-semen
#31
JOURNAL ARTICLE
Wael A Khalil, Amr M I Atta, Ibrahim T El-Ratel, Sameh A Abdelnour, Abdel-Khalek E Abdel-Khalek, Sara F Fouda
The current study aimed to investigate the impact of nano-formulations of clove bud ethanolic extract (CBENF) in the extender on sperm characteristics, antioxidant capacity, oxidative biomarkers, enzymatic activity, apoptosis and fertility of post-thawed rabbit semen. Twelve mature male rabbits semen samples were pooled and cryopreserved in a Tris-egg yolk-based extender containing varying concentrations of CBENF (0, 25, 50, 75 and 100 μg/mL). After the equilibration and freezing-thawing process, CBENF (100 μg /mL) significantly enhanced progressive motility, viability and membrane integrity...
November 29, 2023: Reproduction in Domestic Animals
https://read.qxmd.com/read/38012943/social-egg-freezing-as-ambivalent-materialities-of-aging
#32
JOURNAL ARTICLE
Tannistha Samanta
This commentary explores how the material-nonmaterial transactions around reproduction among women raise paradoxical questions of reproductive autonomy and commercialization of reproduction. Drawing from medical anthropological studies on human reproduction, the technology around social egg freezing has been conceived to proffer ambivalent possibilities of hope, despair, and repair as mature women recalibrate their reproductive identities, especially in pronatalist contexts. Building on the material-discursive critique of the 'material turn', I ask if social egg freezing offers an empowering biological reprieve for women who have 'chosen' a non-normative (i...
December 2023: Journal of Aging Studies
https://read.qxmd.com/read/38005959/effects-of-high-biotin-sample-interference-on-antibody-concentrations-in-sandwich-immunoassays
#33
JOURNAL ARTICLE
Geraldo Balieiro Neto, Jair Rodini Engracia Filho, Fabio Enrique Lemos Budino, Acyr Wanderley de Paula Freitas, Weber Vilas Boas Soares
The use of antimicrobial growth promoters (AGPs) is banned because of problems associated with drug residues in animal products and increased bacterial resistance. The immunization of chickens with specific antigens is a promising strategy for generating specific antibodies that can target a wide range of antibiotic-resistant bacteria and can be used as an alternative to antibiotics. Immunoglobulin Y (IgY) antibodies in a polyclonal antibody (pAb) format, when administered orally, modulate the ruminal microbiome and maintain animal health and performance; however, there are concerns pertaining to protein impurities and biotin concentrations in the samples...
October 24, 2023: Vaccines
https://read.qxmd.com/read/37992605/effect-of-the-substitution-of-butter-by-double-cross-linked-egg-yolk-granules-sodium-alginate-emulsion-gel-on-properties-of-baking-dough-during-frozen-storage
#34
JOURNAL ARTICLE
Hong Zhang, Afeng Wei, Sijie Zhou, Huajiang Zhang, Ning Xia, Jing Wang, Yanqiu Ma, Min Fan
In this study, double cross-linked egg yolk granules (EYGs)/sodium alginate (SA) emulsion gel was constructed and used as butter substitute. The water binding capacity, rheology properties and microstructure of EYGs/SA emulsion gel showed that the network structure tended to be complete when the concentration of SA reached 1% (m/v). SA stabilized the EYGs/SA droplets and enhanced the spatial network structure of emulsion gel. After substitution for butter, the network structure of EYGs/SA emulsion gel with more water bounded and the polyhydroxy structure of SA molecules endowed dough with more water retention capacity...
November 17, 2023: Food Chemistry
https://read.qxmd.com/read/37971663/automated-micro-solid-phase-extraction-clean-up-and-gas-chromatography-tandem-mass-spectrometry-analysis-of-pesticides-in-foods-extracted-with-ethyl-acetate
#35
JOURNAL ARTICLE
Andreas Schürmann, Claudio Crüzer, Veronika Duss, Robin Kämpf, Thomi Preiswerk, Hans-Joachim Huebschmann
Generic extraction methods for the multi-compound pesticide analysis of food have found their solid place in laboratories. Ethyl acetate and acetonitrile extraction methods have been developed as fast and easy to handle standard multi-compound methods, both feature benefits and limitations. The direct injection to gas chromatography can be impaired by a high burden of coextracted matrix, resulting in deterioration of the chromatographic system and matrix effects, requiring frequent maintenance. Therefore, common clean-up methods, such as dispersive solid-phase extraction, freeze-out of fats, or gel permeation chromatography, have been applied in clean-up...
November 16, 2023: Analytical and Bioanalytical Chemistry
https://read.qxmd.com/read/37971560/the-limits-of-stress-tolerance-for-zooplankton-resting-stages-in-freshwater-ponds
#36
JOURNAL ARTICLE
Joana L Santos, Dieter Ebert
In seasonal environments, many organisms evolve strategies such as diapause to survive stressful periods. Understanding the link between habitat stability and diapause strategy can help predict a population's survival in a changing world. Indeed, resting stages may be an important way freshwater organisms can survive periods of drought or freezing, and as the frequency and extent of drought or freezing vary strongly among habitats and are predicted to change with climate change, it raises questions about how organisms cope with, and survive, environmental stress...
November 16, 2023: Oecologia
https://read.qxmd.com/read/37959232/oocyte-cryopreservation-in-women-with-ovarian-endometriosis
#37
REVIEW
Judith-Marie Mifsud, Livia Pellegrini, Mauro Cozzolino
Ovarian endometriosis is a gynecological condition that is closely associated with infertility-from its pathogenesis to treatment modalities, this condition presents a challenge both for patients and clinicians alike when seeking conception, due to low AMH levels, peritoneal inflammation, and the inadvertent removal of healthy ovarian parenchyma at surgery. In fact, around half of endometriosis patients seeking fertility require tertiary-level assisted reproduction techniques to achieve a live birth. Oocyte cryopreservation, a procedure initially designed for oncology patients, has emerged over recent years as a very promising treatment strategy for patients who have been diagnosed with ovarian endometriosis in order to preserve their fertility and obtain a live birth at a later stage in their lives...
October 26, 2023: Journal of Clinical Medicine
https://read.qxmd.com/read/37954402/non-medical-oocyte-cryopreservation-at-a-single-center-in-japan-8%C3%A2-years-of-experience
#38
JOURNAL ARTICLE
Koki Yoshinaga, Tomoko Hashimoto, Yuriko Fukuoka, Noriyuki Okuyama, Koichi Kyono
PURPOSE: Increasing numbers of women are opting to undergo non-medical oocyte cryopreservation (NMOC). In this report, we present experience at our clinic and discuss NMOC in Japan. METHODS: We followed the progress of 403 women who underwent NMOC at our clinic between 2014 and 2021, totaling 592 reproductive cycles. RESULTS: In total, 61 women underwent oocyte warming and fertility treatment. Of these, 13 women gave birth to 14 children. The median age at first oocyte cryopreservation was 38...
2023: Reproductive Medicine and Biology
https://read.qxmd.com/read/37953253/patients-and-providers-perspectives-on-the-decision-to-undergo-non-urgent-egg-freezing-a-needs-assessment
#39
JOURNAL ARTICLE
E Shirin Dason, Leah Drost, Ellen M Greenblatt, Adena Scheer, Jinglan Han, Tanya Doshi, Claire A Jones
BACKGROUND: Previous research has demonstrated that patients have difficulty with the decision to undergo non-urgent egg freezing (EF). This study aimed to investigate the decisional difficulties and possible decisional support mechanisms for patients considering EF, and for their providers. METHODS: This qualitative study involved a needs assessment via individual interviews. Participants included patients considering EF at one academic fertility clinic and providers from across Canada who counsel patients considering EF...
November 13, 2023: BMC Women's Health
https://read.qxmd.com/read/37914489/planned-oocyte-cryopreservation-a-review-of-current-evidence-on-outcomes-safety-and-risks
#40
REVIEW
Bonnie B Song, Molly M Quinn
Although oocyte cryopreservation was initially used as a fertility preservation strategy for medical indications, it is now is increasingly used to circumvent age-related infertility. Outcomes following planned oocyte vitrification, also known as elective egg freezing, are limited. Current studies show higher success rates for individuals undergoing fertility preservation treatment under age 35. Additionally, while freezing 20 oocytes is optimal to achieve pregnancy, freezing at least 8-10 oocytes is recommended...
December 2023: Obstetrics and Gynecology Clinics of North America
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