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Egg freezing

Gabriel Levin, Amihai Rottenstreich
It is a shared belief that women considering fertility preservation should be advised of the exponential decline in their ovarian reserve coupled with a diminished response to ovarian stimulation as they age. We read the review by Alteri et al. with great interest. The authors stretch out the current knowledge on fertility preservation for non-medical indications in women willing to postpone motherhood. While the authors are to be congratulated for their comprehensive overview, a distinct point regarding the optimal timing to freeze oocyte should be clarified...
March 2, 2019: Acta Obstetricia et Gynecologica Scandinavica
Jacob C Eaton, Pamela Rothpletz-Puglia, Margaret R Dreker, Lora Iannotti, Chessa Lutter, Joyceline Kaganda, Pura Rayco-Solon
BACKGROUND: Adequate nutrients early in life promote cognitive development and are critical for proper growth and functioning. The effect of individual nutrients consumed through food is often not the same as consuming the same nutrients in supplementary form due to 'food synergy', the biological and chemical interrelations that occur between nutrients. Animal-source foods, such as eggs, meat, fish, and dairy, are energy dense and contain multiple micronutrients and essential fatty acids with high bioavailability...
February 19, 2019: Cochrane Database of Systematic Reviews
Alessandra Alteri, Valerio Pisaturo, Daniela Nogueira, Arianna D'Angelo
The aim of this review is to provide current knowledge on fertility preservation for nonmedical reasons in women willing to postpone childbearing. The topic is highly debatable starting from the disagreement about its terminology, the number of eggs necessary to predict chances of success, and the safety and socio/ethical point of view. Cost analysis and discrepancies among countries recommendations and regulations are described to confirm the controversies and unsolved issues around this very interesting topic...
February 13, 2019: Acta Obstetricia et Gynecologica Scandinavica
E Maris, A Ferrieres-Hoa, A Gala, A Coffy, E Vintejoux, N Ranisavljevic, S Hamamah
OBJECTIVE: It is already known that children born after slow frozen embryo replacement have a significantly higher birth weight compared to children born after fresh embryo transfer. Similar data have been reported related to frozen embryo transfer using an open vitrification system. However, few data relative to birth weight using a complete embryo closed vitrification system has been reported. The purpose of this study was to know if frozen embryo in closed vitrification system is associated with a higher birth weight compared to fresh embryo replacement...
February 8, 2019: Gynecologie, Obstetrique, Fertilite & Senologie
Iulia Bucur, Sarah Gabriël, Inge Van Damme, Pierre Dorny, Maria Vang Johansen
This study aimed to assess in vitro the transmission potential of T. saginata eggs stored in different media at various temperatures, and to optimize recovery, hatching and activation methods. A total of 42 freshwater and 42 silt samples were spiked with 100 μl of a T. saginata egg suspension, of which half were stored at 5 °C and the other half at 20 °C. Additionally, 5 silt and 35 tap water control samples were included. Eggs were obtained from gravid proglottids passed by a human carrier following treatment...
February 2019: Veterinary Parasitology
Navid Esfandiari, Julia Litzky, Joshua Sayler, Pavel Zagadailov, Karen George, Leslie DeMars
BACKGROUND: Little is known about resident attitudes toward elective egg freezing (EF) or how educational exposure to EF affects residents' views and ability to counsel patients. This study aimed to evaluate US OB/GYN residents' views on elective EF, decisions regarding family planning, and whether education on EF affects these views and self-reported comfort discussing EF with patients. METHODS: A 32 question survey was emailed to program directors at all US residency programs for distribution to residents...
January 29, 2019: Reproductive Biology and Endocrinology: RB&E
Walid S El-Nattat, Reda I El-Sheshtawy
Strawberry fruit is rich in natural antioxidants supplemented as a preservative in semen extenders. There is little literature regarding its application in cattle bulls. So, the aim of the present study was to explore the impact of strawberry juice beside LDL derived from egg yolk on buffalo spermatozoa during cooling and cryopreservation. Collected semen was pooled to be divided in aliquots. Semen was diluted in tris-Citrate-Fructose-yolk low-density lipoprotein (TLCF) (control, 0% strawberry juice (SB)) and in six different concentrations (1-6%) of tris-LDL-strawberry diluent (TLSB) to ensure 60 million motile spermatozoa/mL...
January 28, 2019: Tropical Animal Health and Production
Ana Borovecki, Pamela Tozzo, Nicoletta Cerri, Luciana Caenazzo
In recent years, a social trend toward delaying childbearing has been observed in women of reproductive age. A novel technomedical innovation was commercialized for non-medical reasons to healthy, ostensibly fertile women, who wished to postpone motherhood for various reasons such as educational or career demands, or because they had not yet found a partner. As a consequence, these women may be affected by age-related infertility when they decide to conceive, and fertility preservation techniques can be obtained through the so-called social egg freezing ...
December 20, 2018: Journal of Public Health Research
Cristina Álvarez, Victoria Luño, Noelia González, Lydia Gil
Sperm quality in donkeys (Equus asinus) after freezing thawing is still considered lower than that from other animals, including horses. The aim of this study was to test a new freezing extender supplemented with jenny colostrum on donkey sperm. After thawing, we evaluated sperm motility by means of computer-assisted analysis, viability by SYBR-14 and propidium iodide (PI), membrane functional integrity by HOS-test and acrosome integrity by isothiocyanate conjugated with peanut agglutinin (FITC-PNA) and PI...
January 24, 2019: Cryobiology
Svenja M Ackermann, Dirk W Lachenmeier, Thomas Kuballa, Birk Schütz, Manfred Spraul, Mirko Bunzel
Both the German and European organic food markets are growing fast, and there is also a rising demand for organic chicken eggs. Consumers are willing to pay higher prices for organic eggs produced in an animal appropriate environment considering animal welfare. Strict labelling requirements do not prevent chicken eggs from being a subject of food fraud. Conventionally produced (barn/free-range) eggs can easily be mislabeled as organic eggs. Especially since the demand for organically produced chicken eggs is likely to exceed supply in the future, mislabeling appears to be a realistic scenario...
January 24, 2019: Magnetic Resonance in Chemistry: MRC
Doug Crump, Kim L Williams, Suzanne Chiu, Luke Periard, Robert J Letcher
Double-crested cormorants (DCCO) are piscivorous birds that breed in variably contaminated colonies across the Laurentian Great Lakes of North America. Collection and preparation of environmentally relevant extracts from eggs that contain variable concentrations of organohalogen contaminants (OHCs) represents a minimally invasive approach to characterize potential effects of exposure using in vitro bioassays. In this study, a rapid, efficient lipid freeze-filtration extraction method was used to prepare extracts from DCCO eggs collected from five breeding colonies that had variable OHC burdens...
January 18, 2019: Environmental Toxicology and Chemistry
Osamu Ishihara, Seung Chik Jwa, Akira Kuwahara, Tomonori Ishikawa, Koji Kugu, Rintaro Sawa, Kouji Banno, Minoru Irahara, Hidekazu Saito
Purpose: The Japan Society of Obstetrics and Gynecology started an online cycle-based assisted reproductive technology (ART) registry system in 2007. This report presents the characteristics and treatment outcomes of ART registered for the cycles practiced during 2016. Methods: Cycle-specific information for all ART cycles implemented in participating ART facilities were collected. A descriptive analysis was conducted for the registry database of 2016. Results: In total, 447 790 treatment cycles and 54 110 neonates (one in 18...
January 2019: Reproductive Medicine and Biology
Zeynep B Gürtin, Trina Shah, Jinjun Wang, Kamal Ahuja
RESEARCH QUESTION: What can we learn from 5 years of egg-freezing practice in the UK? What are the different categories of egg freezing, and what are the social and demographic characteristics of patients, and their decisions regarding subsequent storage or thawing? DESIGN: A retrospective analysis of clinical and laboratory data of all 514 cycles of 'own' egg freezing conducted at the London Women's Clinic in the 5-year period from the start of 2012 to the end of 2016...
December 11, 2018: Reproductive Biomedicine Online
Alexis K Masbou, Jenna B Friedenthal, David H McCulloh, Caroline McCaffrey, M Elizabeth Fino, James A Grifo, Frederick Licciardi
Two of the many milestone developments in the field of assisted reproduction have been oocyte donation and preimplantation genetic testing for aneuploidy (PGT-A). Because it has been demonstrated that even young women produce a meaningful proportion of aneuploid embryos, screening out such abnormalities could potentially increase the efficacy of donor egg (DE) cycles. In this retrospective cohort study, we investigated the effect of PGT-A on DE cycle outcomes, including implantation rate (IR), spontaneous abortion rate (SABR), and ongoing pregnancy/live birth rate...
December 20, 2018: Reproductive Sciences
Niloofar Ziarati, Tohid Rezaei Topraggaleh, Pegah Rahimizadeh, Leila Montazeri, Saman Maroufizadeh, Mohammad Ali Sadighi Gilani, Abdolhossein Shahverdi
Application of an appropriate freezing carrier is crucial for improving post-thaw recovery of oligozoospermic samples. In this study, our purpose is developing a user-friendly, easy handling and close micro-quantity (MQ) straw along with different freezing media, for cryopreservation of oligozoospermic samples. Twenty oligozoospermic semen samples were collected and mixed with glycerol egg yolk citrate (GEYC) or Spermfreeze® (SPF) medium. The mixture was loaded into MQ straws, sealed and stored in liquid nitrogen (LN) vapor...
December 8, 2018: Cryobiology
Vinay Gunnala, Jessica Fields, Mohamad Irani, Debra D'Angelo, Kangpu Xu, Glenn Schattman, Zev Rosenwaks
OBJECTIVE: To investigate whether BRCA carriers with and without malignancy have decreased ovarian reserve at baseline compared with BRCA noncarriers. DESIGN: Retrospective cohort study. SETTING: Academic medical center. PATIENT(S): Seven-hundred and ninety-five oocyte cryopreservation patients, comprising BRCA carriers with and without malignancy (n = 57) and BRCA noncarriers (n = 738). INTERVENTION(S): Fertility preservation with oocyte cryopreservation...
December 5, 2018: Fertility and Sterility
Chunrong Lv, Guoquan Wu, Qionghua Hong, Guobo Quan
The cryotolerance of farm animal spermatozoa varies according to their specific features, such as size, shape, and lipid composition. Thus, it is impossible to develop a standardized freezing procedure for different kinds of livestock species. The establishment of an efficient semen cryopreservation procedure will facilitate long-term conservation of small ruminant genetic resources and extension of artificial insemination in daily production. Different from sheep, goat seminal plasma contains a phospholipase, which can affect spermatozoa viability through interaction with milk or egg yolk...
November 30, 2018: Biopreservation and Biobanking
Francesca Filippi, Fabio Martinelli, Alessio Paffoni, Marco Reschini, Francesco Raspagliesi, Edgardo Somigliana
PURPOSE: To evaluate the capacity of random antral follicle count (AFC), i.e., AFC recorded at any time during the menstrual cycle, to predict the number of retrieved mature oocytes in women with malignancies undergoing random start ovarian hyperstimulation METHODS: A consecutive series of 72 women with malignancies who underwent ovarian hyperstimulation aimed at egg freezing between July 2014 and December 2016 was retrospectively reviewed. A standardized random start protocol was used for all women...
November 26, 2018: Journal of Assisted Reproduction and Genetics
Anna Katharina Wöll, Juliane Schütz, Jana Zabel, Jürgen Hubbuch
Knowledge of protein behavior/stability during freeze/thaw (FT) operations is essential for storage and production processes in the biopharmaceutical industry. FT stress involves freeze concentration, cold denaturation, and ice crystals formation which can result in protein aggregation. Therefore, it is important to understand the ongoing FT processes, and the influence of different solution parameters. In order to evaluate the ongoing processes during FT (up to -80°C), phase diagrams with lysozyme from chicken egg white and sodium chloride were generated...
November 17, 2018: International Journal of Pharmaceutics
L Fraser, Ł Zasiadczyk, J Strzeżek, R Strzeżek, K Karpiesiuk
Lipoproteins, isolated from ostrich egg yolk (LPFo), provide excellent protection for boar spermatozoa against cryo-induced damage. The present study was performed to investigate the effects of LPFo on the freezability and fertilizing capacity of frozen-thawed (FT) boar semen after post-cervical artificial inseminations (post-CAIs). Semen, collected from 7 Polish Large White (PLW) and 4 Polish Landrace (PLR), was frozen in an extender containing LPFo. Post-CAIs were performed in 38 multiparous sows, using a catheter-cannula kit...
June 2018: Polish Journal of Veterinary Sciences
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