Daichi M Sakamoto, Iori Tamura, Bo Yi, Sho Hasegawa, Yutaro Saito, Naoki Yamada, Yoichi Takakusagi, Shimpei I Kubota, Minoru Kobayashi, Hiroshi Harada, Kenjiro Hanaoka, Masayasu Taki, Masaomi Nangaku, Kazuki Tainaka, Shinsuke Sando
Elucidation of biological phenomena requires imaging of microenvironments in vivo . Although the seamless visualization of in vivo hypoxia from the level of whole-body to single-cell has great potential to discover unknown phenomena in biological and medical fields, no methodology for achieving it has been established thus far. Here, we report the whole-body and whole-organ imaging of hypoxia, an important microenvironment, at single-cell resolution using activatable covalent fluorescent probes compatible with tissue clearing...
February 1, 2024: ACS Nano