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urinary biomarkers for diabetic nephropathy

Etty Kruzel-Davila, Jasmin Divers, Gregory B Russell, Zipi Kra-Oz, Moran Szwarcwort Cohen, Carl D Langefeld, Lijun Ma, Douglas S Lyles, Pamela J Hicks, Karl L Skorecki, Barry I Freedman
Purpose: African Americans who shed JC polyoma virus (JCV) in their urine have reduced rates of non-diabetic chronic kidney disease (CKD). The present study assessed associations between urinary JCV and urine BK polyomavirus (BKV) with CKD in African Americans with diabetes mellitus. Methods: African Americans with diabetic kidney disease and controls lacking nephropathy from the Family Investigation of Nephropathy and Diabetes Consortium (FIND) and African American-Diabetes Heart Study (AA-DHS) had urine tested for JCV and BKV using quantitative polymerase chain reaction...
January 30, 2019: Journal of Clinical Endocrinology and Metabolism
Hongdi Cao, Jining Wu, Jing Luo, Xiaolan Chen, Junwei Yang, Li Fang
BACKGROUND: Mitochondrial dysfunction and chronic sterile inflammation are common features of type 2 diabetes. Therefore, we aimed to investigate whether mitochondrial DNA (mtDNA) could be a biomarker implicated in the progression of type 2 diabetes and diabetic nephropathy and explore the underlying mechanism. METHOD: We developed a method for relative quantification of mtDNA content in clinical practice. qRT-PCR was used to measure the mtDNA content both in vivo in CD-1 mice with diabetes induction by streptozotocin and in vitro in murine endothelial cells and conditionally immortalized mouse podocytes...
January 24, 2019: Diabetes/metabolism Research and Reviews
Taqwaa Sueud, Najah R Hadi, Raed Abdulameer, Dina A Jamil, Hayder A Al-Aubaidy
AIMS: Diabetic nephropathy (DN) is a serious microvascular complication of a longstanding hyperglycemia. This study aims to evaluate whether urinary neutrophil gelatinase-associated lipocalin (NGAL) and urinary Interleukin-18 possess a better diagnostic value than albumin creatinine ratio in assessing the severity of nephropathy in patients with type 2 diabetes mellitus (T2DM). MATERIAL & METHODS: Ninety participants diagnosed with T2DM were recruited and they were divided into three study groups according to their albumin/creatinine ratio (ACR): (Normoalbuminuria group, Microalbuminuria group, and Macroalbuminuria group)...
January 2019: Diabetes & Metabolic Syndrome
Elisa Bellei, Emanuela Monari, Stefania Bergamini, Luigi Alberto Pini, Tomasi Aldo, Tomris Ozben
Objective: Urinary proteomics is primarily applied to the study of renal and urogenital tract disorders. Here are reported two distinct successful examples of this approach for the discovery of early urinary biomarkers of kidney-related dysfunctions: diabetic nephropathy (DN), a well-known complication of diabetes frequently leading to dialysis, and drug-induced nephrotoxicity, a possible condition caused by medication-overuse headache (MOH). Early detection of kidney disorders based on selective biomarkers could permit to diagnose patients at the initial stage of the disease, where the therapy may be suspended or prevent disease advancement...
December 2018: EJIFCC
Wen-Ling Liao, Chiz-Tzung Chang, Ching-Chu Chen, Wen-Jane Lee, Shih-Yi Lin, Hsin-Yi Liao, Chia-Ming Wu, Ya-Wen Chang, Chao-Jung Chen, Fuu-Jen Tsai
Diabetic nephropathy (DN) is a major complication in diabetic patients. Microalbuminuria testing is used to identify renal disease; however, its predictive value is questionable. We aimed to identify urinary biomarkers to early diagnosis nephropathy before identifiable alternations in kidney function or urine albumin excretion occurs. Proteomic approaches were used to identify potential urinary biomarkers and enzyme-linked immunosorbent assay was performed to verify the results. The data identified haptoglobin (HPT) and α-1-microglobulin/bikunin precursor (AMBP) as two biomarkers with the highest ability to distinguish between healthy individuals and patients with nephropathy, and between diabetic patients with and without DN...
November 26, 2018: Journal of Clinical Medicine
Cinzia Magagnotti, Gianpaolo Zerbini, Isabella Fermo, Rose Mary Carletti, Riccardo Bonfanti, Fabiana Vallone, Annapaola Andolfo
Despite research progresses, the chance to accurately predict the risk for diabetic nephropathy (DN) is still poor. So far, the first evidence of DN is micro-albuminuria, which is detected only 10-20 years after the onset of diabetes. Our goal is to develop new predictive tools of nephropathy starting from urine, which can be easily obtained using noninvasive procedures and it is directly related to kidney. Since it is reasonable to suppose that, in predisposed patients, the mechanisms leading to nephropathy start acting since the diabetes onset, urine from children with recent diagnosis of type 1 diabetes was subjected to proteomic analysis in comparison to age-matched controls...
October 24, 2018: Journal of Proteomics
Chia-Wen Kuo, Hsiao-Ling Chen, Min-Yu Tu, Chuan-Mu Chen
Extracellular superoxide dismutase (SOD3), one member of the antioxidant defense system and a superoxide scavenger, has been noted to be downregulated in the kidneys of diabetic mice and is characterized by a heparin-binding domain that can anchor the protein to the endothelium and extracellular matrix. The association of the serum and urinary SOD3 levels with diabetic nephropathy in different stages has never been evaluated. It remains unclear how urinary SOD3 changes in different renal diseases. We recruited 98 Taiwanese patients with type 2 diabetes and 10 patients with early chronic kidney disease (CKD) into this study...
October 10, 2018: American Journal of Physiology. Renal Physiology
Hideharu Abe, Akiko Sakurai, Hiroyuki Ono, Sanae Hayashi, Sakiya Yoshimoto, Arisa Ochi, Sayo Ueda, Kenji Nishimura, Eriko Shibata, Masanori Tamaki, Fumi Kishi, Seiji Kishi, Taichi Murakami, Kojiro Nagai, Toshio Doi
Diabetic nephropathy (DN) is the major cause of end-stage renal failure and is associated with increased morbidity and mortality as compared to other causes of renal disease. Albuminuria is often the first clinical indicator of the presence of DN. However, albuminuria or proteinuria is a common symptom in patients with various renal disorders. Therefore, specific biomarkers for the diagnosis of DN are required. A primary hallmark of DN is the progressive damage and death of glomerular podocytes, resulting in the leaking of proteins into the urine...
2018: Journal of Medical Investigation: JMI
Chao-Jung Chen, Wen-Ling Liao, Chiz-Tzung Chang, Yu-Ning Lin, Fuu-Jen Tsai
Diabetic nephropathy (DN) is one of the most common complications of diabetes mellitus (DM). To discover early stage biomarkers of DN, untargeted liquid chromatography-mass spectrometry-based metabolomic analysis was performed in urine samples from healthy subjects and patients with micro- or macroalbuminuria due to nondiabetic disease (macro), type 2 DM without microalbuminuria (T2DM), and type 2 DM with microalbuminuria (T2DM+micro). Levels of four metabolites were significantly different among groups, and they were quantified in a larger group of 267 urine samples...
November 2, 2018: Journal of Proteome Research
Jing-Min Zheng, Zuan-Hong Jiang, De-Jun Chen, Sa-Sa Wang, Wen-Jin Zhao, Li-Juan Li
AIMS/INTRODUCTION: The aim of the present study was to obtain a full view of the changes of urinary complement activation products in the development of diabetic nephropathy and explore their possible significance in the disease process. MATERIALS AND METHODS: A total of 62 patients at different stages of diabetic nephropathy, 20 diabetes patients without nephropathy and 20 healthy persons were enrolled. Urinary complement activation products, including C3a, C5a and C5b-9, were measured, and their associations with the progression of the disease were analyzed...
September 21, 2018: Journal of Diabetes Investigation
Jani K Haukka, Niina Sandholm, Carol Forsblom, Jeffrey E Cobb, Per-Henrik Groop, Ele Ferrannini
Elevated urinary albumin excretion (microalbuminuria) is an early marker of diabetic nephropathy, but there is an unmet need for better biomarkers that capture the individuals at risk with higher accuracy and earlier than the current markers do. We performed an untargeted metabolomic study to assess baseline differences between individuals with type 1 diabetes who either developed microalbuminuria or remained normoalbuminuric. A total of 102 individuals progressed to microalbuminuria during a median follow-up of 3...
September 14, 2018: Scientific Reports
P Prabu, S Rome, C Sathishkumar, C Gastebois, E Meugnier, V Mohan, M Balasubramanyam
AIMS: MicroRNAs (miRNAs) from extracellular vesicles (EVs) have been proposed as promising biomarkers for a number of diseases. In this study, their potential as urine-based biomarkers of diabetic nephropathy (DN) was assessed. METHODS: MiRNAs were profiled in urinary EVs from 160 fasting subjects with normal glucose tolerance (NGT) and in T2DM patients with either microalbumininuria (MIC) or macroalbuminuria (MAC). RESULTS: A total of 73 miRNAs detected in urinary EVs (NGT) were predicted to target important functions for kidney homoeostasis, thereby validating their use as indicators of kidney dysfunction...
August 27, 2018: Diabetes & Metabolism
Lili Meng, Guiqin Li, Xiaoqin Liu, Juan Jiang, Min Zhu, Yuanbo Sun
BACKGROUND: The current study aims to evaluate the expression pattern changes of miR-199-3p in diabetic nephropathy (DN) patients and explore whether it could be used as a potential biomarker. METHODS: Real time PCR analysis was performed to examine the level of miR-199-3p in normoalbuminuria group (diabetes mellitus [DM]), microalbuminuria group (DNE), macroalbuminuria group (DNC), and healthy controls. ROC analysis was carried out to determine whether urine miR-199-3p could be used as a potential biomarker...
July 1, 2018: Clinical Laboratory
Yong Chul Kim, Jung Nam An, Jin Hyuk Kim, Young-Wook Choi, Sohee Oh, Sang Ho Kwon, Mi-Young Lee, Junghun Lee, Jae-Gyun Jeong, Chun Soo Lim, Yon Su Kim, Seung Hee Yang, Jung Pyo Lee
Hepatocyte growth factor and its receptor cMet activate biological pathways necessary for repair and regeneration following kidney injury. Here, we evaluated the clinical role of urinary cMet as a prognostic biomarker in diabetic nephropathy (DN). A total of 218 patients with DN were enrolled in this study. We examined the association of urine cMet levels and long-term outcomes in patients with DN. The levels of urinary cMet were higher in patients with decreased renal function than in patients with relatively preserved renal function (5...
August 24, 2018: Scientific Reports
Nicole Endlich, Tim Lange, Jana Kuhn, Paul Klemm, Ahmed M Kotb, Florian Siegerist, Frances Kindt, Maja T Lindenmeyer, Clemens D Cohen, Andreas W Kuss, Neetika Nath, Rainer Rettig, Uwe Lendeckel, Uwe Zimmermann, Kerstin Amann, Sylvia Stracke, Karlhans Endlich
Podocyte loss and changes to the complex morphology are major causes of chronic kidney disease (CKD). As the incidence is continuously increasing over the last decades without sufficient treatment, it is important to find predicting biomarkers. Therefore, we measured urinary mRNA levels of podocyte genes NPHS1, NPHS2, PODXL and BDNF, KIM-1, CTSL by qRT-PCR of 120 CKD patients. We showed a strong correlation between BDNF and the kidney injury marker KIM-1, which were also correlated with NPHS1, suggesting podocytes as a contributing source...
November 2018: Journal of Cellular and Molecular Medicine
Takeshi Osonoi, Maki Gouda, Mamiko Kubo, Kenji Arakawa, Toshio Hashimoto, Masanori Abe
BACKGROUND: Albuminuria characterizes the progression of kidney injury. The effect of canagliflozin on the excretion of microalbumin was assessed for investigating its renoprotective potential in Japanese patients with type 2 diabetes mellitus (T2DM). PATIENTS AND METHODS: Twenty Japanese patients with T2DM and microalbuminuria were enrolled and administered with 100 mg of canagliflozin once a day for 12 weeks. These subjects were admitted to the clinic at the start and end of the treatment period for 24-h urine collection...
October 2018: Diabetes Technology & Therapeutics
Felix S Seibert, Maximilian Sitz, Jürgen Passfall, Martin Haesner, Peter Laschinski, Martin Buhl, Frederic Bauer, Nina Babel, Nikolaos Pagonas, Timm H Westhoff
BACKGROUND/AIMS: Urinary biomarkers like neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1) do not only allow an early diagnosis of acute kidney injury, but also provide prognostic information in this setting. The present prospective study investigates, whether the urinary biomarkers NGAL, KIM-1 and calprotectin have prognostic information in chronic kidney disease (CKD) as well. METHODS: Urinary calprotectin, NGAL and KIM-1 concentrations were assessed in a study population of 143 patients with stable CKD comprising diabetic and hypertensive nephropathy, glomerulonephritis/vasculitis, and autosomal dominant polycystic kidney disease...
2018: Kidney & Blood Pressure Research
So Young Kim, Tae Dong Jeong, Woochang Lee, Sail Chun, Sung Sunwoo, Soon Bae Kim, Won Ki Min
BACKGROUND: An increase in neutrophil gelatinase-associated lipocalin (NGAL) indicates tubular injury. Diabetic nephropathy causes typical changes in the kidney, characterized by glomerulosclerosis and eventual tubular damage. We validated the usefulness of plasma NGAL (pNGAL) as a biomarker of tubular damage in patients with diabetic nephropathy. METHODS: We included 376 patients with diabetes mellitus (260 patients with chronic renal insufficiency who had not received hemodialysis and 116 hemodialyzed due to diabetic nephropathy) and 24 healthy controls...
November 2018: Annals of Laboratory Medicine
Xiaoqing Ni, Yunjuan Gu, Haoyong Yu, Shenqi Wang, Ying Chen, Xinlei Wang, Xinlu Yuan, Weiping Jia
Serum fatty acid-binding protein 4 (FABP4) has been linked to renal dysfunction. This study evaluated the association between serum FABP4 and the radioisotope glomerular filtration rate (rGFR) in type 2 diabetic patients (T2DM) with early diabetic nephropathy. Twenty healthy controls and 172 patients with T2DM were enrolled. Serum FABP4 and renal impairment biomarkers including urinary albumin-to-creatinine ratio (UACR), serum retinal-binding protein 4 (RBP4), urinary cystatin C-to-creatinine ratio (CysC/Cr), and neutrophil gelatinase-associated lipocalin-to-creatinine ratio (NGAL/Cr) were measured...
2018: BioMed Research International
Aleksandra Klisic, Nebojsa Kavaric, Ana Ninic
Background: Since the increase in some tubular damage biomarkers can be observed at the early stage of diabetic nephropathy, even in the absence of albuminuria, we aimed to investigate if urinary albumin is superior than tubular damage marker, such as serum retinol-binding protein 4 (RBP4), in predicting renal function decline (defined as estimated glomerular filtration rate [eGFR] <60 mL/min/1.73 m2 ) in the cohort of patients with type 2 diabetes mellitus (T2D). Materials and Methods: A total of 106 sedentary T2D patients (mean [± standard deviation] age 64...
2018: Journal of Research in Medical Sciences: the Official Journal of Isfahan University of Medical Sciences
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