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p38 MAPK AP-1

Mingyeong Kang, See-Hyoung Park, Se Jung Park, Sae Woong Oh, Ju Ah Yoo, Kitae Kwon, Jangsoon Kim, Eunbi Yu, Jae Youl Cho, Jongsung Lee
BACKGROUND: Melanin plays a crucial role in protecting human skin against exposure to ultraviolet (UV) radiation. However, its overproduction induces hyperpigmentation disorders of the skin. PURPOSE: To investigate effects of phenylethyl resorcinol as one resorcinol derivative on melanogenesis and its mechanisms using B16F10 mouse melanoma cells and human epidermal melanocytes. METHODS: Effects of phenylethyl resorcinol on melanogenesis and its mechanism of action were examined using several in vitro assays (i...
February 26, 2019: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Qingyun Jia, Tengteng Wang, Xiaoyun Wang, Hao Xu, Yang Liu, Yongjun Wang, Qi Shi, Qianqian Liang
Astragalin, as a bioactive flavonoid with anti-inflammatory, antioxidant, and protective properties, provides a potential agent for rheumatoid arthritis (RA). In this study, its therapeutic efficacy and the underlying mechanisms were explored using DBA/1J mice with collagen-induced arthritis (CIA). It was demonstrated that astragalin could significantly attenuate inflammation of CIA mice. The effects were associated with decreased severity of arthritis (based on the arthritis index), joint swelling and reduced bone erosion and destruction...
2019: Frontiers in Pharmacology
Rashmi Nagesh, K M Kiran Kumar, M Naveen Kumar, Rajeshwari H Patil, S Chidananda Sharma
Areca nut chewing habits are associated with several oral manifestations like leukoplakia, submucous fibrosis and oral squamous cell carcinoma. Although numerous evidence on areca toxicity is known but the mechanistic pathway of disease causation is to be studied. Aqueous areca nut extract treated A549 cells showed reduced cell viability by 48 h with IC50 value of 0.50%. The toxic nature of areca nut induced the production of reactive oxygen species with decreased anti-oxidant glutathione S transferase levels lead to altered redox homeostasis...
February 2, 2019: Cytotechnology
Shigeki Sakai, Atsushi Nishida, Masashi Ohno, Osamu Inatomi, Shigeki Bamba, Mitsushige Sugimoto, Masahiro Kawahara, Akira Andoh
Astaxanthin is a xanthophyll carotenoid, which possesses strong scavenging effect on reactive oxygen species. In this study, we examined the effect of astaxanthin on dextran sulfate sodium (DSS)-induced colitis in mice. Experimental colitis was induced by the oral administration of 4% w/v DSS in tap water in C57BL/6J mice. Astaxanthin was mixed with a normal rodent diet (0.02 or 0.04%). Astaxanthin significantly ameliorated DSS-induced body weight loss and reduced the disease activity index. The ameliorating effects was observed in a dose-dependent manner...
January 2019: Journal of Clinical Biochemistry and Nutrition
Gan Luo, Brian Chi-Yan Cheng, Hui Zhao, Xiu-Qiong Fu, Ran Xie, Shuo-Feng Zhang, Si-Yuan Pan, Yi Zhang
Schisandra Fructus (SF) is a traditional Chinese herb used in the treatment of inflammatory disorders like hepatitis. One of the main anti-inflammatory components of SF is the lignans. However, the underlying anti-inflammatory mechanism of Schisandra Chinensis lignans (SCL) remains unclear. This study aims to investigate the effects of SCL on inflammatory mediators in lipopolysaccharide-stimulated RAW264.7 cells and explore the underlying mechanism. The production of nitric oxide (NO) was determined by Griess reaction...
December 14, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Xingmao Wu, Kaiqiang Ji, Haiyuan Wang, Yang Zhao, Jia Jia, Xiaopeng Gao, Bin Zang
OBJECTIVE: We aimed to investigate the association of macrophage inflammatory protein (MIP)-1α (CCL3) expression with the severity of acute pancreatitis (AP). METHODS: The patients with AP were selected and divided into mild AP (MAP), moderately severe AP (MSAP), and severe AP (SAP) groups according to the severity of AP. The pancreatic acinar cell line Ar42 j was treated with cerulein to induce in vitro cell AP model. The expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and the activation of the c-Jun N-terminal kinase (JNK)/p38 mitogen-activated protein kinase (MAPK) signaling pathway in stimulated or transfected Ar42 j cells were detected...
December 14, 2018: Journal of Cellular Biochemistry
Seung-Shick Shin, Myeong-Cheol Ko, Dae-Hwa Noh, Byungdoo Hwang, Yujin Park, Sung Lyea Park, Wun-Jae Kim, Sung-Kwon Moon
Hyper-proliferation and migration of vascular smooth muscle cells (VSMCs) are closely associated with atherosclerosis. Recently, the flavonol morin has been reported to exhibit potent anti-oxidant and anti-inflammatory activities. Therefore, we investigated molecular mechanisms of morin in VSMCs stimulated by PDGF. Morin effectively inhibited PDGF-stimulated proliferation of VSMCs through a G1 cell-cycle arrest, leading to down-regulation of CDK2, CDK4, cyclin D1, and cyclin E proteins. Interestingly, PDGF markedly down-regulated p27KIP1 protein expression; however, morin treatment restored the p27KIP1expression to the basal level...
September 2018: General Physiology and Biophysics
Xi Duan, Tao Wu, Ting Liu, Hao Yang, Xiaojie Ding, Yan Chen, Yunzhu Mu
Exposure of ultraviolet (UV) radiation is the major factor from environment to cause erethema, inflammation, photoaging and skin carcinogenesis. Vicenin-2, a bioflavonoid which are isolated from several medicinal plants. The present study was demonstrated the role of vicenin-2 on UVB linked oxidative stress and photoaging signaling in human dermal fibroblasts (HDF). In this study, UVB-irradiation (100 mJ/cm2 ) significantly elevated the intracellular ROS levels, lipid peroxidation, DNA damage, depletion of antioxidants resulted in apoptotic HDF cells...
November 24, 2018: Journal of Photochemistry and Photobiology. B, Biology
Mohammed Youssef, Ahmed Ibrahim, Koichi Akashi, Md Shamim Hossain
The special lipids plasmalogens (Pls) were reported to be reduced in the neurodegenerative brains such as Alzheimer's disease where a marked increase of glial activation is often observed. We previously found that a reduction of brain Pls can enhance the glial activation in murine brains. However, the detailed role of Pls in the prevention of glial activation was mostly elusive. Here we report that the Pls, extracted from scallop (sPls), significantly inhibited the inducible form of nitric oxide synthase (NOS2) and the production of NO in LPS (lipopolysaccharide)-activated microglial cells...
January 15, 2019: Neuroscience
Xue Tian, Guogang Xie, Fengming Ding, Xin Zhou
AIM OF THE STUDY: Matrix metalloproteinases (MMPs) play a critical role in chronic obstructive pulmonary disease (COPD). This study investigated the role of mitogen-activated protein kinases (MAPKs) in MMP-9 secretion of BEAS-2B cells, a human bronchial epithelial cell line and U937 cells, a human myeloid leukaemia cell line, which could differentiate into macrophage, after LPS stimulation, and some details of involved signaling. MATERIALS AND METHODS: MTT assay was used to measure cell viability...
November 23, 2018: Experimental Lung Research
Dong Oh Kim, Jae-Eun Byun, Hyun-A Seong, Suk Ran Yoon, Inpyo Choi, Haiyoung Jung
Inflammation comprises an innate immune response, and is mainly induced by macrophages to protect the host from pathogens and mechanical injuries. The p38 mitogen-activated protein kinase (MAPK) pathway is a key regulator of inflammatory responses in macrophages. Here, we investigated the anti-inflammatory effects of thioredoxin-interacting protein-derived peptide (TN13) in macrophages in vitro and in vivo. Human immunodeficiency virus (HIV) trans-activator protein (TAT)-conjugated TN13 (TAT-TN13) was found to penetrate RAW 264...
December 9, 2018: Biochemical and Biophysical Research Communications
Congjie Bi, Qiuping Cai, Yangyang Shan, Fan Yang, Shiwei Sun, Xiuying Wu, Hongtao Liu
As one of the most popular anesthetics, sevoflurane is widely used in pediatric anesthesia. Unfortunately, an increasing number of studies have demonstrated that sevoflurane has potential neurotoxic effects on the developing brain and cognition, even in adolescence. Connexin 43 (Cx43) has been documented to contribute to cognitive dysfunction. The present study hypothesized that Cx43 may participate in sevoflurane-induced neuroinjury and investigated the underlying mechanisms in young Sprague Dawley (SD) rats...
December 2018: Biomedicine & Pharmacotherapy
Ming Wang, Shuhong An, Diyi Wang, Haizhen Ji, Xingjing Guo, Zhaojin Wang
A previous study showed that a downexpression of protease-activated receptor 4 (PAR4) is associated with the development of esophageal squamous cell carcinoma (ESCC). In this study, we explored the relationship between PAR4 activation and the expression of p16, and elucidated the underlying mechanisms in PAR4 inducing the tumor suppressor role in ESCC. ESCC cell lines (EC109 and TE-1) were treated with PAR4-activating peptide (PAR4-AP). Immunohistochemistry for DNA methyltransferase 1 (DNMT1) and histone deacetylase 2 (HDAC2) was performed in 26 cases of ESCC tissues...
2018: Journal of Immunology Research
Angélique Arcambal, Janice Taïlé, Philippe Rondeau, Wildriss Viranaïcken, Olivier Meilhac, Marie-Paule Gonthier
Type 2 diabetes is associated with major vascular dysfunctions, leading to clinical complications such as stroke. It is also known that hyperglycemia dysregulates blood-brain barrier homeostasis by altering cerebral endothelial cell function. Oxidative stress may play a critical role. The aim of this study was to evaluate the effect of hyperglycemia and insulin on the production of redox, inflammatory and vasoactive markers by cerebral endothelial cells. Murine bEnd.3 cerebral endothelial cells were exposed to hyperglycemia in the presence or not of insulin...
October 23, 2018: Free Radical Biology & Medicine
Trong Thuan Ung, Thi Thinh Nguyen, Sen Lian, Shinan Li, Yong Xia, Nam Ho Kim, Young Do Jung
Interleukin-6 (IL-6), a pleiotropic cytokine, plays a key role in endothelial injury and atherosclerosis. In this study, we investigated the effects of nicotine, a major psychoactive compound in cigarette smoke, on IL-6 expression and EA.hy926 endothelial cell invasion. Nicotine stimulated IL-6 expression via the activator protein 1 (AP-1) transcription factor. Pharmacological inhibition and mutagenesis studies indicated that p38 mitogen-activated protein kinase (MAPK) mediated the IL-6-induced upregulation of nicotine in EA...
October 14, 2018: Journal of Cellular Biochemistry
I-Ta Lee, Chih-Chung Lin, Chien-Chung Yang, Li-Der Hsiao, Ming-Yen Wu, Chuen-Mao Yang
Staphylococcus aureus ( S. aureus ) is a very common Gram-positive bacterium. It is widely distributed in air, soil, and water. S. aureus often causes septicemia and pneumonia in patients. In addition, it is considered to play a key role in mediating cell adhesion molecules upregulation. Resveratrol is a natural antioxidant with diverse biological effects, including the modulation of immune function, anti-inflammation, and cancer chemoprevention. In this study, we proved that S. aureus -upregulated vascular cell adhesion molecule-1 (VCAM-1) expression in human lung epithelial cells (HPAEpiCs) was inhibited by resveratrol...
October 7, 2018: International Journal of Molecular Sciences
Dong Yeong Min, Euitaek Jung, Juhwan Kim, Young Han Lee, Soon Young Shin
Leptin, an adipokine regulating energy metabolism, appears to be associated with breast cancer progression. Insulin-like growth factor-1 (IGF-1) mediates the pathogenesis of breast cancer. The regulation of IGF-1 expression by leptin in breast cancer cells is unclear. Here, we found that leptin upregulates IGF-1 expression at the transcriptional level in breast cancer cells. Activating protein-1 (AP-1)-binding element within the proximal region of IGF-1 was necessary for leptin-induced IGF-1 promoter activation...
October 8, 2018: BMB Reports
Yujia Bai, Yunyao Jiang, Tingwu Liu, Fu Li, Jianmei Zhang, Yanyan Luo, Liang Zhang, Guilong Yan, Zuoshan Feng, Xueqin Li, Xinfeng Wang, Weicheng Hu
ETHNOPHARMACOLOGICAL RELEVANCE: A multi-herb Chinese medicinal formula consisting of a variety of medicinal and edible materials has long been consumed as a hot drink and immune enhancer for its efficiency to increase disease resistance in Xinjiang, China. However, no fundamental data has been collected associated with traditional consumption. The present work was designed to evaluate the immunostimulatory role of Xinjiang herbal tea (XMT-WE) in RAW 264.7 macrophages and cyclophosphamide (CTX)-induced immunosuppression mice model...
September 27, 2018: Journal of Ethnopharmacology
Li Zhao, Tao Zhang, Hao Geng, Zhi-Qi Liu, Zhao-Feng Liang, Zhi-Qiang Zhang, Jie Min, De-Xin Yu, Cai-Yun Zhong
Bladder cancer is the most common malignancy of the urinary tract. Long-term exposure to benzidine is one of the major causes of bladder cancer. However, the mechanism of benzidine-induced bladder cancer is not yet sufficiently characterized. Dysregulated cell proliferation serves a critical role in cancer initiation and development; whether benzidine promotes cell proliferation, and the role of MAPKs in this process, have not previously been investigated. The present study aimed to investigate the benzidine-induced modulation of intracellular mitogen-activated protein kinases (MAPKs) and activator protein-1 (AP-1) signaling cascades on cell proliferation in SV-40 immortalized human uroepithelial cells (SV-HUC-1)...
October 2018: Oncology Letters
Yao-Xin Gao, Hao-Heng Yu, Chuan He, Ming Li, Dan-Dan Guo, Jun-Jiang Lian, Hai-Jie Yang, Mian Wang, Lei Wang, Zhi-Wei Feng, Bin-Feng Cheng
Background and purpose: Fengshi Gutong capsule (FSGTC), a traditional herbal formula, has been used clinically in China for the treatment of arthritis. However, the mechanism underlying the therapeutic effects of FSGTC on osteoarthritis (OA) has not been elucidated. The present study investigated the function and mechanisms of FSGTC in rat OA model and interleukin (IL)-1β-stimulated synovial cells. Materials and methods: Rat OA model was established by intra-articular injection containing 4% papain. IL-1β-induced SW982 cells were used as an OA cell model...
2018: Frontiers in Pharmacology
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