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c-Jun keratinocyte

Xiaoxue Jiang, Huan He, Zhehui Xie, Huimin Wen, Xiaoran Li, Xin Li, Jun Ma, Kazuo Umezawa, Yuyang Zhang
BACKGROUND: Dehydroxymethylepoxyquinomicin (DHMEQ) is a specific and potent inhibitor of nuclear factor-kappa B (NF-κB) and has been shown to possess promising potential as an anti-inflammation including anti-atopic dermatitis (AD)-like skin lesions. OBJECTIVE: To further evaluate the activity of DHMEQ in vivo modified AD-like lesion model in BALB/c mice and in vitro AD-like lesion cell model in human keratinocytes. MATERIALS AND METHODS: In this study, in vivo modified AD-like lesion model in BALB/c mice was chronically induced by the repetitive and alternative application of 2,4-dinitrochlorobenzene (DNCB) and oxazolone (OX) on ears, and stratum corneum of the ear skin was additionally stripped off with surgical tapes before each challenge with DNCB/OX...
February 6, 2019: Immunopharmacology and Immunotoxicology
Pei-Wen Wang, Yu-Chen Cheng, Yu-Chiang Hung, Chih-Hung Lee, Jia-You Fang, Wen-Tai Li, Yun-Ru Wu, Tai-Long Pan
Extensive exposure to UVB (280-320 nm) is the major risk responsible for various skin injuries. Numerous reports have shown that natural products could demonstrate photochemopreventive efficacy against UVB damage. We investigated the preventive effects and associated molecular mechanisms of red raspberry extract upon UVB-caused damage in human epidermal keratinocytes and a nude mouse model. The protein profiles and immunohistological study on a nude mouse skin indicated that red raspberry extract could prevent UVB-caused cell death and protect the skin against UVB-exposed injury manifested by wrinkling, scaling, tanning, and water loss as well as epidermal thickening...
2019: Oxidative Medicine and Cellular Longevity
Hui Wang, Shujing Wei
This study investigated the effects of tanshinol (TAN) on lipopolysaccharide (LPS)-induced human keratinocytes inflammatory injury and underlying potential molecular mechanisms. Viability and apoptosis of HaCaT cells were assessed using MTT assay and Annexin V-FITC/PI staining, respectively. Quantitative reverse transcription-polymerase chain reaction was performed to measure the expression of microRNA-122 (miR-122) in HaCaT cells. Cell transfection was conducted to up-regulate the expression of miR-122. Western blotting was used to detect the protein expression levels of key factors involved in cell apoptosis, inflammatory response, c-Jun N-terminal kinase (JNK), and nuclear factor kappa B (NF-κB) pathways...
January 10, 2019: Phytotherapy Research: PTR
Yin Chu, Po-Yuan Wu, Chien-Wen Chen, Jia-Ling Lyu, Yi-Jung Liu, Kuo-Ching Wen, Chien-Yih Lin, Yueh-Hsiung Kuo, Hsiu-Mei Chiang
The skin provides an effective barrier against physical, chemical, and microbial invasion; however, overexposure to ultraviolet (UV) radiation causes excessive cellular oxidative stress, which leads to skin damage, DNA damage, mutations, and skin cancer. This study investigated the protective effects of N -phenethyl caffeamide (K36) from UVA damage on human epidermal keratinocytes. We found that K36 reduced UVA-induced intracellular reactive oxygen species (ROS) production and induced the expression of the intrinsic antioxidant enzyme heme oxygenase-1 (HO-1) by increasing the translocation of nuclear factor erythroid 2⁻related factor 2 (Nrf2)...
January 4, 2019: International Journal of Molecular Sciences
Kyu-Shik Lee, Min-Gu Lee, Yun-Jeong Woo, Kyung-Soo Nam
Ultraviolet light (UV) is a major inducer of skin cancer. Therefore, recovery and removal of UV-damaged skin cells are important in the prevention of skin carcinogenesis. Here, we investigated the effect of deep sea water (DSW) in HaCaT keratinocyte exposed by UVB (λ = 290∼320 nm). The result showed that UVB-induced cell death was reinforced by DSW treatment in a hardness-dependent manner. Furthermore, the increase of cell death by DSW was associated with the down-regulation of survivin and RAD51 expressions induced by UVB...
December 24, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Julie H Wu, Deepika Narayanan, Rebecca A Simonette, Peter L Rady, Stephen K Tyring
A number of pruritic skin conditions arising in immunocompromised patients are associated with viral infection. Recently, human polyomavirus 7 (HPyV7) has been implicated in the pathogenesis of eruptive pruritic parakeratotic and dyskeratotic dermatoses with distinct "peacock plumage" histology. While expression of HPyV7 viral protein, namely small tumor (sT) antigen, is prominent within lesional tissue, the functional role of HPyV7 in cutaneous pathobiology is not yet known. In this study, we demonstrate a novel role for HPyV7 sT antigen in pathways important for the maintenance of keratinocyte structure and function...
December 10, 2018: International Journal of Dermatology
Byung-Cheol Lee, Jisun Song, Arim Lee, Daeho Cho, Tae Sung Kim
Visfatin, a member of the adipokine family, plays an important role in many metabolic and stress responses. The mechanisms underlying the direct therapeutic effects of visfatin on wound healing have not been reported yet. In this study, we examined the effects of visfatin on wound healing in vitro and in vivo. Visfatin enhanced the proliferation and migration of human dermal fibroblasts (HDFs) and keratinocytes the expression of wound healing-related vascular endothelial growth factor (VEGF) in vitro and in vivo...
November 19, 2018: International Journal of Molecular Sciences
Rebecca Duit, Tim J Hawkins, Arto Määttä
Thioglycolate is a potent depilatory agent. In addition, it has been proposed to be useful as a penetration enhancer for transepidermal drug delivery. However, the effects on hair structure and stress responses it elicits in epidermal keratinocytes have not been fully characterized. We have used label-free confocal and fluorescence lifetime imaging supported by electron microscopy to demonstrate how thioglycolate damages hair cuticle cells by generating breakages along the endocuticle and leading to swelling of cortex cells...
November 11, 2018: Experimental Dermatology
Xiao Jiang, Fan Wu, Yuan Xu, Jian-Xin Yan, Yin-Di Wu, Sheng-Hong Li, Xuan Liao, Jun-Xian Liang, Ze-Hua Li, Hong-Wei Liu
BACKGROUND: Recent evidence suggests that angiotensin II (Ang II) plays a role in cutaneous wound healing. Mesenchymal stem cells (MSCs) are known as a rich source of cells that re-establish healed skin. However, the potential impact of Ang II on MSC differentiation into keratinocytes is still unknown. OBJECTIVE: The present study was conducted to explore the effect of Ang II on differentiation of bone marrow-derived MSCs (BM-MSCs) into keratinocytes. METHODS: BM-MSCs were isolated from rat bone marrow and cultured...
November 9, 2018: Experimental Dermatology
Jeffrey B Cheng, Andrew J Sedgewick, Alex I Finnegan, Paymann Harirchian, Jerry Lee, Sunjong Kwon, Marlys S Fassett, Justin Golovato, Matthew Gray, Ruby Ghadially, Wilson Liao, Bethany E Perez White, Theodora M Mauro, Thaddeus Mully, Esther A Kim, Hani Sbitany, Isaac M Neuhaus, Roy C Grekin, Siegrid S Yu, Joe W Gray, Elizabeth Purdom, Ralf Paus, Charles J Vaske, Stephen C Benz, Jun S Song, Raymond J Cho
Perturbations in the transcriptional programs specifying epidermal differentiation cause diverse skin pathologies ranging from impaired barrier function to inflammatory skin disease. However, the global scope and organization of this complex cellular program remain undefined. Here we report single-cell RNA sequencing profiles of 92,889 human epidermal cells from 9 normal and 3 inflamed skin samples. Transcriptomics-derived keratinocyte subpopulations reflect classic epidermal strata but also sharply compartmentalize epithelial functions such as cell-cell communication, inflammation, and WNT pathway modulation...
October 23, 2018: Cell Reports
Ganlan Bian, Caiyong Yu, Ling Liu, Chao Fang, Kun Chen, Pan Ren, Qian Zhang, Fangfang Liu, Kun Zhang, Qian Xue, Jie Xiang, Hongmin Guo, Jun Song, Yu Zhao, Wutian Wu, Sookja K Chung, Ruixia Sun, Gong Ju, Jian Wang
In many mammals, the eyelids migrate over the eye and fuse during embryogenesis to protect the cornea from damage during birth and early life. Loss-of-function mutations affecting the epidermal growth factor receptor (EGFR) signaling pathway cause an eyes-open-at-birth (EOB) phenotype in rodents. We identified an insertional mutation in Spinster homolog 2 ( Spns2 ) in a strain of transgenic rats exhibiting the EOB phenotype. Spns2, a sphingosine 1-phosphate (S1P) transporter that releases S1P from cells, was enriched at the tip of developing eyelids in wild-type rat embryos...
October 23, 2018: Science Signaling
Hana Petra Decean, Ioana Carmen Brie, Corina Bianca Tatomir, Maria Perde-Schrepler, Eva Fischer-Fodor, Piroska Virag
Ultraviolet B radiation (UVB) activates mitogen-activated protein kinases (MAPK): p38, extracellular signal regulated (ERK), and c-Jun N-terminal (JNK) kinases in human skin cells. Human keratinocytes (KC) exposed to UVB secrete several cytokines (CK), among which the growth differentiation factor-15 (GDF-15) is augmented in inflammatory and aging processes and the granulocyte macrophage-colony stimulating factor (GM-CSF) is involved in cell proliferation, differentiation, and survival, and both CK have implications in skin carcinogenesis...
2018: Journal of Environmental Pathology, Toxicology and Oncology
C M Kim, S H Jeong, H Lee, H J Ryu, S W Son
BACKGROUND: Silver nanoparticles (Ag-NPs) can prevent bacterial infection and improve cutaneous wound healing owing to their antimicrobial activity. However, the mechanism of their antimicrobial activity is poorly understood. AIM: To determine the mechanistic relationship between Ag-NP treatment and expression of psoriasin. METHODS: Human epidermal keratinocytes, neonatal (HEKn) were used. Psoriasin mRNA expression was measured by reverse transcription PCR and real-time PCR...
September 24, 2018: Clinical and Experimental Dermatology
Min Zhang, Yuehou Zhang, Jun Ding, Xiaohong Li, Chengyu Zang, Siyuan Yin, Jiaxu Ma, Yibing Wang, Yongqian Cao
CD271, a receptor of nerve growth factor (NGF), affects the biological properties of epidermal stem cells (eSCs) which are essential for skin wound closure. Tropomyosin-receptor kinase A (TrkA), another receptor of NGF, combined with CD271 has been involved with nervous system and skin keratinocytes. However, the exact role of TrkA combined with CD271 in eSCs during skin wound closure is still unclear. This study aimed to reveal the role of TrkA in the promoting wounding-healing effect of CD271 on eSCs. We obtained CD271-vo (over-expression of CD271) eSCs by lentiviral infection...
September 12, 2018: Archives of Dermatological Research
Yihua Cai, Feng Xue, Chen Quan, Minye Qu, Na Liu, Yuan Zhang, Chris Fleming, Xiaoling Hu, Huang-Ge Zhang, Ralph Weichselbaum, Yang-Xin Fu, David Tieri, Eric C Rouchka, Jie Zheng, Jun Yan
IL-1 signaling pathway has been shown to play a critical role in the pathogenesis of chronic, autoinflammatory skin diseases such as psoriasis. However, the exact cellular and molecular mechanisms have not been fully understood. Here, we show that IL-1β is significantly elevated in psoriatic lesional skin and imiquimod (IMQ)-treated mouse skin. In addition, IL-1R signaling appears to correlate with psoriasis disease progression and treatment response. IL-1 signaling in both dermal γδ T cells and other cells such as keratinocytes is essential to an IMQ-induced skin inflammation...
August 15, 2018: Journal of Investigative Dermatology
In Wook Kim, Hyo-Soon Jeong, Nyoun Soo Kwon, Kwang Jin Baek, Hye-Young Yun, Dong-Seok Kim
Leucine-rich repeat LGI family member 3 (LGI3), a member of the LGI family, is a secreted protein that is expressed not only in the brain and adipose tissues, but also in various skin cells. We previously reported that LGI3 was secreted after exposure to ultraviolet B and promoted the migration of HaCaT human keratinocytes. In the present study, we investigated whether LGI3 influences the differentiation of keratinocytes. The results show that the expression of involucrin, a keratinocyte differentiation marker, was reduced in tissue from LGI3-knockout mice...
August 9, 2018: Experimental Dermatology
Chun-Hsun Huang, Li-Chun Chang, Sindy Hu, Chien-Yu Hsiao, Shu-Ju Wu
Spilanthol has been reported to possess antioxidant, anti‑inflammatory, antimicrobial and antinociceptive properties. At present, the literature has reported the beneficial role of spilanthol on tumor necrosis factor‑α (TNF‑α)‑stimulated HaCaT cells. The present study investigated the effects of spilanthol on the expression of TNF‑α‑induced intercellular adhesion molecule 1 (ICAM‑1) and cyclooxygenase (COX)‑2 in the human keratinocyte cell line HaCaT. Cells were pretreated with various concentrations of spilanthol (10‑150 µM) followed by TNF‑α to induce inflammation...
September 2018: Molecular Medicine Reports
Gyoseon Goya M Ryeom, Weon Jeong Bang, Young Bu Kim, Go Eun Lee
Tannins are a type of polyphenols found in several fruits such as grapes and berries, and nuts such as aronias and acorns. Both hydrolyzable tannins and condensed tannins are referred to as tannins. Among the hydrolyzable tannins, gallotannin has a strong antioxidative property and is known to protect the skin by inhibiting the precursors of elastolytic enzymes. However, its mechanism of protection against ultraviolet B (UVB) damage in human fibroblasts and keratinocytes has not yet been elucidated. In this study, we investigate the antioxidant and antiaging effect of gallotannin on UVB-irradiated human cells by studying its effect on extracellular signal-regulated kinases/c-Jun N-terminal kinases (EKRs/JNKs) signaling related to cell growth and differentiation/stress apoptosis...
August 2018: Journal of Medicinal Food
Tsong-Min Chang, Ting-Ya Yang, Yu-Lin Niu, Huey-Chun Huang
Dictamni dasycarpus is a type of Chinese medicine made from the root bark of D. dasycarpus . It has been reported to show a wide spectrum of biological and pharmacological effects, for example, it has been used widely for the treatment of rheumatism, nettle rash, itching, jaundice, chronic hepatitis and skin diseases. In the current study, D. dasycarpus extract was investigated for its antioxidant and anti-inflammatory effects, as well as its capability to alleviate oxazolone-induced skin damage in mice. The possible anti-inflammatory mechanism of D...
June 15, 2018: Antioxidants (Basel, Switzerland)
Jun Kurai, Masanari Watanabe, Hiroyuki Sano, Kyoko Iwata, Degejirihu Hantan, Eiji Shimizu
Ambient particulate matter (PM) can increase airway inflammation and induce bronchoconstriction in asthma. This study aimed to investigate the effect of tiotropium bromide, a long-acting muscarinic antagonist, on airway inflammation and bronchoconstriction induced by ambient PM in a mouse model of asthma. We compared the effect of tiotropium bromide to that of fluticasone propionate and formoterol fumarate. BALB/c mice were sensitized to ovalbumin (OVA) via the airways and then administered tiotropium bromide, fluticasone propionate, or formoterol fumarate...
June 6, 2018: International Journal of Environmental Research and Public Health
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