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Mitsuo Sakamoto, Nao Ikeyama, Takumi Murakami, Hiroshi Mori, Masahiro Yuki, Moriya Ohkuma
Previous analyses based on 16S rRNA and hsp60 genes indicated that Parolsenella catena and Libanicoccus massiliensis were closely related to each other and formed a monophyletic cluster independent of the related Olsenella species. To clarify the relationship of these two species, we determined the genome sequence of P. catena JCM 31932T and compared it with that already sequenced for L. massiliensis Marseille-P3237T . Phylogenetic trees based on the concatenated 37 single-copy ribosomal proteins or RpoB robustly supported the relationship observed in the previous studies...
February 14, 2019: International Journal of Systematic and Evolutionary Microbiology
Weronika Gonciarz, Agnieszka Matusiak, Karolina Rudnicka, Tomasz Rechciński, Maciej Chałubiński, Elżbieta Czkwianianc, Marlena Broncel, Adrian Gajewski, Magdalena Chmiela
Helicobacter pylori (Hp) may initiate autoimmunity as a result of molecular mimicry. The aim of this study was to compare the level of IgG antibodies to a specific epitope (P1 peptide) of human heat shock protein (Hsp)60 homologous to Hp Hsp60 (HspB) in the sera of healthy donors (HD), patients with Hp-related gastritis or coronary heart disease (CHD), uninfected or with Hp infection confirmed by rapid urease test, histological examination (dyspeptic patients) the 13 C urea breath test (13 C UBT), and anti-Hp antibodies (healthy donors, CHD patients)...
March 2019: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
Zlata Gvozdenov, Janhavi Kolhe, Brian C Freeman
Maintenance of a healthy and functional proteome in all cellular compartments is critical to cell and organismal homeostasis. Yet, our understanding of the proteostasis process within the nucleus is limited. Here, we discuss the identified roles of the major molecular chaperones Hsp90, Hsp70, and Hsp60 with client proteins working in diverse DNA-associated pathways. The unique challenges facing proteins in the nucleus are considered as well as the conserved features of the molecular chaperone system in facilitating DNA-linked processes...
February 11, 2019: Cold Spring Harbor Perspectives in Biology
Tianqi Li, Haoran Yu, Yangyang Song, Ruili Zhang, Ming Ge
Several studies have been conducted on liver damage caused by cadmium, but few on the protective effects of Ganoderma triterpenoids against liver damage due to cadmium. This experiment was designed to evaluate the protective effects of Ganoderma triterpenoids on the liver damage induced by cadmium in chickens. Eighty healthy seven-day-old Hyline male egg-laying chickens were randomly divided into four groups with 20 chickens in each group. All the experiments were carried out in triplicate. The control group (K group) was fed a basal diet, the Cadmium group (Cd group) was fed a basal diet with 140 mg/kg of CdCl2 , the Ganoderma triterpenoids treatment group (Cd + GT group) was fed with a full-fodder diet containing 140 mg/kg of CdCl2 and 0...
March 2019: Journal of Trace Elements in Medicine and Biology
Morihiko Nakamura, Kaori Notsu, Mai Nakagawa
Monoclonal nonspecific suppressor factor β (MNSFβ) is a ubiquitously expressed ubiquitin-like protein known to be involved in various biological functions. Previous studies have demonstrated that MNSFβ covalently modify its target proteins including Bcl-G, a proapoptotic protein. In this study, we purified a 65 kDa MNSFβ adduct from mouse liver lysates by sequential chromatography on DEAE and glutathione S-transferase (GST)-fusioned MNSFβ immobilized on glutathione-Sepharose beads in the presence of ATP...
February 1, 2019: Molecular and Cellular Biochemistry
Tiphaine Le Roy, Patrick Van der Smissen, Adrien Paquot, Nathalie Delzenne, Giulio G Muccioli, Jean-François Collet, Patrice D Cani
A Gram-negative, strictly anaerobic, non-spore forming, non-motile, non-pigmented bacterial strain, designated H184T , was isolated from human faeces. 16S rRNA gene sequence analysis showed that strain H184T represents a member of the genus Butyricimonas. Strain H184T is related to but distinct from Butyricimonasvirosa JCM 15149T and Butyricimonasparavirosa JCM 18677T , with 16S rRNA gene sequence similarities of 96.32 and 96.24 %, respectively. Strain H184T shared 90.50 % hsp60 gene sequence similarity to B...
January 30, 2019: International Journal of Systematic and Evolutionary Microbiology
Kristina Wardelmann, Sabine Blümel, Michaela Rath, Eugenia Alfine, Chantal Chudoba, Mareike Schell, Weikang Cai, Robert Hauffe, Kathrin Warnke, Tanina Flore, Katrin Ritter, Jürgen Weiß, C Ronald Kahn, André Kleinridders
OBJECTIVE: Insulin action in the brain controls metabolism and brain function, which is linked to proper mitochondrial function. Conversely, brain insulin resistance associates with mitochondrial stress and metabolic and neurodegenerative diseases. In the present study, we aimed to decipher the impact of hypothalamic insulin action on mitochondrial stress responses, function and metabolism. METHODS: To investigate the crosstalk of insulin action and mitochondrial stress responses (MSR), namely the mitochondrial unfolded protein response (UPRmt) and integrated stress response (ISR), qPCR, western blotting, and mitochondrial activity assays were performed...
January 6, 2019: Molecular Metabolism
R Akhi, C Wang, A E Nissinen, J Kankaanpää, R Bloigu, S Paju, P Mäntylä, K Buhlin, J Sinisalo, P J Pussinen, S Hörkkö
A large body of literature has established the link between periodontal disease and cardiovascular disease. Oxidized low-density lipoproteins (OxLDLs) have a crucial role in atherosclerosis progression through initiation of immunological response. Monoclonal IgM antibodies to malondialdehyde-modified low-density lipoprotein (MDA-LDL) and to malondialdehyde acetaldehyde-modified low-density lipoprotein (MAA-LDL) have been shown to cross-react with the key virulence factors of periodontal pathogens Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans...
January 22, 2019: Journal of Dental Research
Huang He, Jiao Xiaoyan, Xu Yanmin, Han Qi, Jiao Wanying, Liu Yanyan, Shu Li, Teng Xiaohua
Lead (Pb) is an environmental pollutant and can damage organisms. Selenium (Se) can alleviate Pb poisoning. The present study aimed to investigate alleviative effect of Se on Pb-caused immune toxicity in chicken hearts. One hundred and eighty Hy-line male chickens were randomly divided into four groups at 7 days of age. The control group was offered a standard commercial diet (SD) and drinking water (DW); the Se group was offered SD supplemented with sodium selenite (SeSD) and DW; the Pb + Se group was offered SeSD and DW supplemented with lead acetate (PbDW); and the Pb group was offered SD and PbDW...
January 21, 2019: Avian Pathology: Journal of the W.V.P.A
José Ángel Fernández-Higuero, Isabel Betancor-Fernández, Noel Mesa-Torres, Arturo Muga, Eduardo Salido, Angel L Pey
To carry out their biological function in cells, proteins must be folded and targeted to the appropriate subcellular location. These processes are controlled by a vast collection of interacting proteins collectively known as the protein homeostasis network, in which molecular chaperones play a prominent role. Protein homeostasis can be impaired by inherited mutations leading to genetic diseases. In this chapter, we focus on a particular disease, primary hyperoxaluria type 1 (PH1), in which disease-associated mutations exacerbate protein aggregation in the cell and mistarget the peroxisomal alanine:glyoxylate aminotransferase (AGT) protein to mitochondria, in part due to native state destabilization and enhanced interaction with Hsp60, 70 and 90 chaperone systems...
2019: Advances in Protein Chemistry and Structural Biology
Thaís Larré Oliveira, Caroline Rizzi, Carlos Eduardo Pouey da Cunha, Jessica Dorneles, Amilton Clair Pinto Seixas Neto, Marta Gonçalves Amaral, Daiane Drawanz Hartwig, Odir Antônio Dellagostin
Leptospirosis is a zoonosis that is responsible for one million human cases per year. Fusing multiple immunogenic antigens represents a promising approach to delivering an effective vaccine against leptospirosis. Mycobacterium bovis bacillus Calmette-Guérin (BCG) is a potential vaccine vector due to its adjuvant properties and safety. Two chimeric genes based on genic sequences of ligANI, ligBrep, lipL32, and lemA, were individually cloned into five BioBrick vectors with different promoters (pAN, Hsp60, 18 kDa, Ag85B and Ag85B plus signal sequence) for antigen expression in BCG...
January 7, 2019: Vaccine
Eleanne F van Ess, Anat Eck, Jolande A Land, Servaas A Morré, Sander Ouburg
PROBLEM: Tubal factor infertility (TFI) is a severe complication of genital C. trachomatis infections. In fertility work-up chlamydia antibody test (CAT) is used to predict TFI. The predictive value for TFI of most commonly used CAT is moderate. METHOD OF STUDY: 183 infertile Dutch Caucasian women were included in this study. All underwent tubal patency testing (HSG or laparoscopy). Cases had TFI, controls had no TFI (i.e. normal findings during HSG or laparoscopy)...
January 10, 2019: American Journal of Reproductive Immunology: AJRI
Joanna Bajzert, Michał Gorczykowski, Tadeusz Stefaniak
Salmonella Enteritidis is one of the most common causes of food poisoning in humans. Many attempts have been made to develop an effective vaccine against S. Enteritidis for use in poultry, but experiments aimed at the complete elimination of this pathogen from poultry farms have not provided satisfactory results. The development of new generation vaccines against salmonellosis, such as subunit vaccines based on heat shock proteins (HSPs), is strongly justified. The high immunogenicity of Hsp60 isolated from Procaryota, including Salmonella, has been suggested by the presence of IgG anti-Hsp60 antibodies in mice immunized with these proteins...
January 4, 2019: Microbial Pathogenesis
Aggeliki Makri, Charalampos Siristatidis, Charalampos Chrelias, Chrysi Christodoulaki, Nikolaos Evangelinakis, Dimitrios Kassanos, Evangelos J Giamarellos-Bourboulis, Aikaterini Pistiki
PROBLEM: To study the balance of circulating heat shock protein (HSP)60 and HSP70 in preterm delivery METHOD OF STUDY: A two-stage approach was used. At first stage we run retrospective analysis of prospective collected clinical data and at a second stage we studied an animal model of preterm delivery (PTD). Blood samples were collected for prenatal screening in 3,629 women. Samples from 23 women with miscarriage before gestational week 21 and 53 well-matched comparators for age, body mass index, parity and previous miscarriage with full-term pregnancy were depicted...
January 4, 2019: American Journal of Reproductive Immunology: AJRI
Ge Cui, Wen Ming Feng, Ting Zhang, Guo Liang Zhu, Qi Lin Shi, Xiao Lan Zhang, Hui Xia
Objective To verify the expressions of genes associated with colorectal cancer with hyperglycemia and evaluate their diagnostic values.Methods Tumor tissues,distal normal intestinal mucosa,and peripheral blood samples were harvested from 109 colorectal cancer patients and peripheral blood samples from 30 diabetes patients and 30 healthy volunteers. The mRNA expressions of glucose regulated protein 78 (GRP78),NADPH oxidase-1 (NOX1),carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5),heat shock protein 60 (HSP60),and histone deacetylase 1(HDAC1) were detected by real-time quantitative polymerase chain reaction...
December 20, 2018: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
Anja-Martina Rottach, Hannes Ahrend, Benedikt Martin, Reinhard Walther, Uwe Zimmermann, Martin Burchardt, Matthias B Stope
PURPOSE: Cabazitaxel, a semi-synthetic taxane of the third generation, inhibits prostate cancer (PC) cell growth by affecting the microtubule architecture. Since cabazitaxel has also been demonstrated to inhibit androgen receptor (AR) functionality, AR and AR-associated heat shock protein (HSP) expressions in the presence of cabazitaxel were characterized. METHODS: AR and HSP expressions were assessed via Western blotting utilizing a PC-cell-line in vitro system incubated with cabazitaxel...
January 2, 2019: World Journal of Urology
Sajan C Achi, Prakash M Halami
In the present study, probiotic, safety and functional characteristics of eight indigenous bifidobacterial isolates were compared to identify suitable strains for functional food application. Among the isolates, six strains of Bifidobacterium longum and one each of Bifidobacterium breve and Bifidobacterium bifidum were identified by 16S rRNA, xfp and hsp60 gene sequencing. Diversity among these strains was established by RAPD and Rep-PCR. Genes associated with sortase-dependent pili (SDP) (credited for role in adhesion) and serpin (immunomodulation) which can serve as potential marker genes for rapid identification of probiotic Bifidobacterium, was also evaluated...
January 2, 2019: Current Microbiology
Xin-Lei Li, Yong-Ling Wang, Jing Zheng, Yang Zhang, Xiao-Feng Zhang
Accumulating evidences suggest that heat shock protein 60 (HSP60) and toll-like receptor 4 (TLR4) are involved in triggering inflammatory response in microglia. Paraquat (PQ) evokes microglial inflammation by up-regulating expression of HSP60- TLR4-myeloid differentiation factor 88 (Myd88)-nuclear factor-kappa B (NF-κB) in vitro. The aim of this study is to investigate the potential modulatory roles of HSP60 and TLR4 in PQ-induced inflammation. Before treated with PQ, microglia BV2 cells were pretreated using siRNA to knockdown HSP60 or with specific inhibitor to inhibit TLR4 expression...
December 22, 2018: Chemico-biological Interactions
Antonella Marino Gammazza, Vincenzo Restivo, Roberta Baschi, Celeste Caruso Bavisotto, Angelo B Cefalù, Giulia Accardi, Everly Conway de Macario, Alberto J L Macario, Francesco Cappello, Roberto Monastero
Molecular chaperones play essential roles in many processes such as cell differentiation, tissue homeostasis, and organ remodeling. Recent data indicate that chaperones can act as cytoprotectants for brain cells during the progression of neurodegenerative diseases, including Alzheimer's disease (AD). However, very few data on the levels of chaperones in dementia, including its prodromal phases, have been reported. In this study, we used biological samples and epidemiological data collected during the Zabùt Aging Project (a prospective, community-based, cohort study of normal/pathological aging conducted in Sicily, Italy, with a follow-up of ten years) to determine if there is an association between plasma levels of the chaperones Hsp60, Hsp70, and Hsp90 with amnestic mild cognitive impairment (aMCI) and AD...
December 20, 2018: Journal of Alzheimer's Disease: JAD
Fang Mao, Yuxin Liu, Liyi Ma, Lidong Liu, Anqi Jiang, Xuejiao Zhai, Jing Zhou
Using metal oxide semiconductor nanomaterials for synergistic cancer treatment has recently attracted the attention of numerous researchers. Herein, oxygen-defective vanadium oxide nanodots (VOx NDs) with ultra-small size and great dispersibility were synthesized via a novel user-friendly method, and then doxorubicin was loaded onto the VOx NDs surfaces. The VOx NDs had great photothermal conversion efficiency and stability. Doxorubicin-loaded VOx NDs can simultaneously serve as therapeutic agent and tumor microenvironment-activable HSP60 inhibitor, resulting in improved efficacy of photothermal therapy and released active doxorubicin for chemotherapy...
December 20, 2018: Biomaterials
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