Caiming Li, Nan Kang, Shun Ye, Weihang Huang, Xia Wang, Cheng Wang, Yuchen Li, Yan-Fei Liu, Ying Lan, Liang Ma, Yuhang Zhao, Yong Han, Jun Fu, Danhua Shen, Lianhua Dong, Wenbin Du
Digital PCR (dPCR) holds immense potential for precisely detecting nucleic acid markers essential for personalized medicine. However, its broader application is hindered by high consumable costs, complex procedures, and restricted multiplexing capabilities. To address these challenges, an all-in-one dPCR system is introduced that eliminates the need for microfabricated chips, offering fully automated operations and enhanced multiplexing capabilities. Using this innovative oscillation-induced droplet generation technique, OsciDrop, this system supports a comprehensive dPCR workflow, including precise liquid handling, pipette-based droplet printing, in situ thermocycling, multicolor fluorescence imaging, and machine learning-driven analysis...
March 22, 2024: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)