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JOURNAL ARTICLE
Shouyan Wu, Kai Liu, Xiaojuan Huang, Qiuyu Sun, Xiaomei Wu, Khalid Mehmood, Ying Li, Hui Zhang
Thiram is a kind of organic compound, which is commonly used for sterilization, insecticidal and deodorization in daily life. Its toxicology has been broadly studied. Recently, more and more microRNAs have been shown to participate in the regulation of cartilage development. However, the potential mechanism by which microRNA regulates chondrocyte growth is still unclear. Our experiments have demonstrated that thiram can hamper chondrocytes development and cause a significant increase in miR-203a content in vitro and in vivo trials...
March 2024: Pesticide Biochemistry and Physiology
#2
JOURNAL ARTICLE
Lucinda Kurzava Kendall, Yuexi Ma, Tony Yang, Katarzyna Lubecka, Barbara Stefanska
The crosstalk between oncogenic signaling pathways plays a crucial role in driving cancer development. We previously demonstrated that dietary polyphenols, specifically resveratrol (RSV) and other stilbenoids, epigenetically target oncogenes for silencing via DNA hypermethylation in breast cancer. In the present study, we identify signal transduction regulators among RSV-hypermethylated targets and investigate the functional role of RSV-mediated DNA hypermethylation in the regulation of Hedgehog and Wnt signaling...
February 29, 2024: Nutrients
#3
JOURNAL ARTICLE
Barbara Łasut-Szyszka, Agnieszka Gdowicz-Kłosok, Beata Małachowska, Małgorzata Krześniak, Agnieszka Będzińska, Marta Gawin, Monika Pietrowska, Marek Rusin
Transcriptomic analyses have revealed hundreds of p53-regulated genes; however, these studies used a limited number of cell lines and p53-activating agents. Therefore, we searched for candidate p53-target genes by employing stress factors and cell lines never before used in a high-throughput search for p53-regulated genes. We performed RNA-Seq on A549 cells exposed to camptothecin, actinomycin D, nutlin-3a, as well as a combination of actinomycin D and nutlin-3a (A + N). The latter two substances synergise upon the activation of selected p53-target genes...
March 7, 2024: Chemico-biological Interactions
#4
JOURNAL ARTICLE
Ernestine Saumweber, Slim Mzoughi, Arin Khadra, Anja Werberger, Sven Schumann, Ernesto Guccione, Michael J Schmeisser, Susanne J Kühl
Mutations in PRDM15 lead to a syndromic form of holoprosencephaly (HPE) known as the Galloway-Mowat syndrome (GAMOS). While a connection between PRDM15, a zinc finger transcription factor, and WNT/PCP signaling has been established, there is a critical need to delve deeper into their contributions to early development and GAMOS pathogenesis. We used the South African clawed frog Xenopus laevis as the vertebrate model organism and observed that prdm15 was enriched in the tissues and organs affected in GAMOS...
2024: Frontiers in Cell and Developmental Biology
#5
JOURNAL ARTICLE
Yangshuai Gao, Yuming Zou, Dimitri Sokolowskei, Xin Xing, Robert J Tower, Zejia Lai, Jiawei Shi, Liheng Zhu, Quan Zheng, Aaron W James, Jiajia Xu, Zhongmin Zhang
Intense inflammatory response impairs bone marrow mesenchymal stem cell (BMSC)-mediated bone regeneration, with TGF-β1 being the most highly expressed cytokine. However, how to find effective and safe means to improve bone formation impaired by excessive TGF-β1 remains unclear. In this study, we found that the expression of orphan nuclear receptor Nr4a1, an endogenous repressor of TGF-β1, was suppressed directly by TGF-β1-induced Smad3 and indirectly by Hdac4, respectively. Importantly, Nr4a1 overexpression promoted BMSC osteogenesis and reversed TGF-β1-mediated osteogenic inhibition and pro-fibrotic effects...
February 29, 2024: Molecular Therapy
#6
JOURNAL ARTICLE
Yalin Zhang, Yujie Zhuang, Jin Zhou, Xiaoyu Xie, Mingzhu Sun, Mengyao Zheng, Keyun Yuan, Zhiping Zhang, Juntao Zhang
Endometritis is a disease caused by a postpartum bacterial infection with a poor prognosis that primarily affects dairy cows. Three-dimensional organoids have been used as a model for endometritis, because they exhibit a structure comparable to that of the endometrium, demonstrating both expansibility and hormone responsiveness. These characteristics render them an ideal platform for in vitro investigations of endometrial diseases. Estradiol (E2) is an endogenous steroid hormone with demonstrated anti-inflammatory properties, and the objective of this study was to determine the mechanism by which E2 modulates the inflammatory response and the Wnt signal transduction pathway in bovine endometrial epithelial cells and organoids following E...
February 22, 2024: Theriogenology
#7
JOURNAL ARTICLE
Gonzalo Schierano-Marotti, Gabriela A Altamirano, Sofia Oddi, Ayelen L Gomez, Nicole Meyer, Mónica Muñoz-de-Toro, Ana C Zenclussen, Horacio A Rodríguez, Laura Kass
Pubertal mammary branching morphogenesis is a hormone-regulated process susceptible to exposure to chemicals with endocrine disruptive capacity, such as the UV-filter benzophenone-3 (BP3). Our aim was to assess whether intrauterine or in vitro exposure to BP3 modified the branching morphogenesis of the female mouse mammary gland. For this, pregnant mice were dermally exposed to BP3 (0.15 or 50 mg/kg/day) from gestation day (GD) 8.5 to GD18.5. Sesame oil treatment served as control. Changes of the mammary glands of the offspring were studied on postnatal day 45...
February 20, 2024: Toxicology and Applied Pharmacology
#8
JOURNAL ARTICLE
Jiangqin Cao, Fatima El Mansouri, Sofia Reynoso, Zongping Liu, Jiaqiao Zhu, Teruko Taketo
Sry on the Y-chromosome upregulates Sox9, which in turn upregulates a set of genes such as Fgf9 to initiate testicular differentiation in the XY gonad. In the absence of Sry expression, genes such as Rspo1, Foxl2, and Runx1 support ovarian differentiation in the XX gonad. These two pathways antagonize each other to ensure the development of only one gonadal sex in normal development. In the B6.YTIR mouse, carrying the YTIR-chromosome on the B6 genetic background, Sry is expressed in a comparable manner with that in the B6...
February 20, 2024: Biology of Reproduction
#9
JOURNAL ARTICLE
Shuai Song, Xiaokai Zhang, Zihang Huang, Yongchao Zhao, Shuyang Lu, Linqi Zeng, Fengze Cai, Tongyao Wang, Zhiqiang Pei, Xinyu Weng, Wei Luo, Hao Lu, Zilun Wei, Jian Wu, Peng Yu, Li Shen, Xiaochun Zhang, Aijun Sun, Junbo Ge
Cardiac fibroblasts (CFs) are the primary cells tasked with depositing and remodeling collagen and significantly associated with heart failure (HF). TEAD1 has been shown to be essential for heart development and homeostasis. However, fibroblast endogenous TEAD1 in cardiac remodeling remains incompletely understood. Transcriptomic analyses revealed consistently upregulated cardiac TEAD1 expression in mice 4 weeks after transverse aortic constriction (TAC) and Ang-II infusion. Further investigation revealed that CFs were the primary cell type expressing elevated TEAD1 levels in response to pressure overload...
February 19, 2024: Signal Transduction and Targeted Therapy
#10
JOURNAL ARTICLE
Shafira Meidyana, Soraya Isfandiary, Relly Yanuari Primariawan
IMPORTANCE: This systematic review supports the involvement of the WNT4 gene in the pathophysiology of endometriosis. OBJECTIVE: To conduct a systematic review and meta-analysis on WNT4 rs7521902 and rs16826658 polymorphism associated with endometriosis based on multi-ethnic case-control studies. DATA SOURCES: Comprehensive searching was performed using Medline, Embase, and Google Scholar. STUDY SELECTION AND SYNTHESIS: Keywords used for searching using Boolean operators are endometriosis, WNT4, and polymorphism...
February 2, 2024: European Journal of Obstetrics, Gynecology, and Reproductive Biology
#11
JOURNAL ARTICLE
Mihaela Pavličev, Caitlin E McDonough-Goldstein, Andreja Moset Zupan, Lisa Muglia, Yueh-Chiang Hu, Fansheng Kong, Nagendra Monangi, Gülay Dagdas, Nina Zupančič, Jamie Maziarz, Debora Sinner, Ge Zhang, Günter Wagner, Louis Muglia
The common human SNP rs3820282 is associated with multiple phenotypes including gestational length and likelihood of endometriosis and cancer, presenting a paradigmatic pleiotropic variant. Deleterious pleiotropic mutations cause the co-occurrence of disorders either within individuals, or across population. When adverse and advantageous effects are combined, pleiotropy can maintain high population frequencies of deleterious alleles. To reveal the causal molecular mechanisms of this pleiotropic SNP, we introduced this substitution into the mouse genome by CRISPR/Cas 9...
February 12, 2024: Nature Communications
#12
JOURNAL ARTICLE
Yinglin Lu, Ming Li, Heng Cao, Jing Zhou, Fan Li, Debing Yu, Minli Yu
OBJECTIVE: The objective of this study was to investigate the regulation relationship of Ten-eleven translocation 1 (Tet1) in DNA demethylation and the proliferation of PGCs in chickens. METHODS: siRNA targeting Tet1 was used to transiently knockdown the expression of Tet1 in chicken PGCs, and the genomic DNA methylation status was measured. The proliferation of chicken PGCs was detected by flow cytometry analysis and cell counting kit-8 (CCK8) assay when activation or inhibition of Wnt4/β-catenin signaling pathway...
January 20, 2024: Animal bioscience
#13
JOURNAL ARTICLE
Serrena Singh, Tifanny Budiman, David Redmond, Vikas Gupta
BACKGROUND: The matrix and associated mesenchyme of the extrahepatic bile ducts are distinct, which could drive diseases with a predilection for these ducts, such as primary sclerosing cholangitis. We aimed to understand the molecular drivers of peribiliary mesenchymal cell (PMC) identity in the extrahepatic bile ducts and dissect how this changed in the context of injury using an entirely in vivo approach with transcriptomic analysis. METHODS AND RESULTS: Single-cell sequencing with a receptor-ligand analysis showed that PMCs had the most interactions with surrounding cells...
February 1, 2024: Hepatology Communications
#14
JOURNAL ARTICLE
Wanlu Ren, Jianwen Wang, Yaqi Zeng, Tongliang Wang, Jun Meng, Xinkui Yao
Kazakh mares have attracted widespread attention with their outstanding lactation traits. Lactation is a complex dynamic process regulated by multiple factors. The extensive application of transcriptome sequencing technology enables researchers to further explore this biological issue. This study selected three pregnant and three non-pregnant Kazakh mares as the research subject. Their mammary glands were taken for transcriptome sequencing. The results show that there are 9 lncRNAs and 122 mRNAs differentially expressed between the two groups...
January 19, 2024: Gene
#15
JOURNAL ARTICLE
Zhenyuan Qian, Wenfa Lin, Xufan Cai, Jianzhang Wu, Kun Ke, Zaiyuan Ye, Fang Wu
Gastric cancer (GC) has been a major health burden all over the world but there are fewer promising chemotherapeutic drugs due to its multidrug resistance. It has been reported that WYC-209 suppresses the growth and metastasis of tumor-repopulating cells but the effect on GC was not explored. MTT, colony formation, and transwell assays were performed to examine the effects of WYC-209 on the proliferation, colony growth, and mobility of GC cells. Western blotting and qRT-PCR were used to detect the expression of proteins and mRNA...
December 31, 2024: Cancer Biology & Therapy
#16
JOURNAL ARTICLE
Xiao Zhang, Hanrui Lin, Da-Li Zheng, You-Guang Lu, Yuchun Zou, Bohua Su
OBJECTIVES: This study aimed to investigate the functions of 19 types of Wnt ligands during the process of osteogenic differentiation in human periodontal ligament stem cells (hPDLSCs), with particular attention to WNT3A and WNT4. MATERIALS AND METHODS: The expression levels of 19 types of Wnt ligands were examined using real-time quantitative polymerase chain reaction (real-time qPCR) during hPDLSCs osteogenic differentiation at 7, 10, and 14 days...
December 30, 2023: Clinical Oral Investigations
#17
JOURNAL ARTICLE
Vahidreza Karamad, Fatma Sogutlu, Ferhat Can Ozkaya, Behrouz Shademan, Weaam Ebrahim, Mona El-Neketi, Cigir Biray Avci
Dysregulation of the Wnt signaling pathway contributes to the development of many cancer types. Natural compounds produced with biotechnological systems have been the focus of research for being a new drug candidate both with unlimited resources and cost-effective production. In this study, it was aimed to reveal the effects of isopropylchaetominine on cytotoxic, cytostatic, apoptotic and Wnt signaling pathways in brain, pancreatic and prostate cancer. The IC50 values of isopropylchaetominine in U-87 MG, PANC1, PC3 and LNCaP cells were calculated as 91...
December 27, 2023: Fitoterapia
#18
JOURNAL ARTICLE
Michael J Mosca, Zhiming He, Florante R Ricarte, Carole Le Henaff, Nicola C Partridge
Teriparatide (PTH (1-34)), PTHrP (1-36), and abaloparatide (ABL) have been used for the treatment of osteoporosis, but their efficacy long term is significantly limited. The 3 peptides exert time- and dose-dependent differential responses in osteoblasts, leading us to hypothesize they may also differentially modulate the osteoblast transcriptome. Treatment of mouse calvarial osteoblasts with 1 nM of the peptides for 4 hours results in RNA sequencing data with PTH (1-34) regulating 367 genes, including 194 unique genes; PTHrP (1-36) regulating 117 genes, including 15 unique genes; and ABL regulating 179 genes, including 20 unique genes...
December 1, 2023: Journal of the Endocrine Society
#19
JOURNAL ARTICLE
Korbinian M Riedhammer, Thanh-Minh T Nguyen, Can Koşukcu, Julia Calzada-Wack, Yong Li, Nurit Assia Batzir, Seha Saygılı, Vera Wimmers, Gwang-Jin Kim, Marialena Chrysanthou, Zeineb Bakey, Efrat Sofrin-Drucker, Markus Kraiger, Adrián Sanz-Moreno, Oana V Amarie, Birgit Rathkolb, Tanja Klein-Rodewald, Lillian Garrett, Sabine M Hölter, Claudia Seisenberger, Stefan Haug, Pascal Schlosser, Susan Marschall, Wolfgang Wurst, Helmut Fuchs, Valerie Gailus-Durner, Matthias Wuttke, Martin Hrabe de Angelis, Jasmina Ćomić, Özlem Akgün Doğan, Yasemin Özlük, Mehmet Taşdemir, Ayşe Ağbaş, Nur Canpolat, Naama Orenstein, Salim Çalışkan, Ruthild G Weber, Carsten Bergmann, Cecile Jeanpierre, Sophie Saunier, Tze Y Lim, Friedhelm Hildebrandt, Bader Alhaddad, Lina Basel-Salmon, Yael Borovitz, Kaman Wu, Dinu Antony, Julia Matschkal, Christian W Schaaf, Lutz Renders, Christoph Schmaderer, Manuel Rogg, Christoph Schell, Thomas Meitinger, Uwe Heemann, Anna Köttgen, Sebastian J Arnold, Fatih Ozaltin, Miriam Schmidts, Julia Hoefele
Congenital anomalies of the kidney and urinary tract (CAKUT) are the predominant cause for chronic kidney disease below age 30 years. Many monogenic forms have been discovered due to comprehensive genetic testing like exome sequencing. However, disease-causing variants in known disease-associated genes only explain a proportion of cases. Here, we aim to unravel underlying molecular mechanisms of syndromic CAKUT in three unrelated multiplex families with presumed autosomal recessive inheritance. Exome sequencing in the index individuals revealed three different rare homozygous variants in FOXD2, encoding a transcription factor not previously implicated in CAKUT in humans: a frameshift in the Arabic and a missense variant each in the Turkish and the Israeli family with segregation patterns consistent with autosomal recessive inheritance...
December 26, 2023: Kidney International
#20
JOURNAL ARTICLE
Alfiya Oskarovna Iusupova, Nikolay Nikolaevich Pakhtusov, Olga Alexandrovna Slepova, Yuri Nikitich Belenkov, Elena Vitalievna Privalova, Irina Vladimirovna Bure, Ekaterina Alexandrovna Vetchinkina, Marina Vyacheslavovna Nemtsova
UNLABELLED: MicroRNAs and the WNT signaling cascade regulate the pathogenetic mechanisms of atherosclerotic coronary artery disease (CAD) development. OBJECTIVE: To evaluate the expression of microRNAs (miR-21a, miR-145, and miR-221) and the role of the WNT signaling cascade (WNT1, WNT3a, WNT4, and WNT5a) in obstructive CAD and ischemia with no obstructive coronary arteries (INOCA). METHOD: The cross-sectional observational study comprised 94 subjects...
December 18, 2023: International Journal of Molecular Sciences
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