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Diego Filipe Bezerra Silva, Hellen Bandeira de Pontes Santos, Jorge Esquiche León, Daliana Queiroga de Castro Gomes, Pollianna Muniz Alves, Cassiano Francisco Weege Nonaka
Spindle cell squamous cell carcinoma of the tongue is a rare variant of squamous cell carcinoma. This paper reports the case of a spindle cell squamous cell carcinoma of the tongue, in a 64-year-old male patient, and presents a review of the etiopathogenesis, clinicopathological and immunohistochemical features and treatment of the malignancy. The patient presented for evaluation of a painful swelling on his tongue. Extraoral examination revealed palpable submandibular and superior cervical lymph nodes. Based on the presumptive diagnoses of squamous cell carcinoma or malignant salivary gland neoplasm, an incisional biopsy was performed...
February 18, 2019: Einstein
Jun Guo, Chunting Zhao, Ruyong Yao, Aihua Sui, Lingjie Sun, Xiaodan Liu, Shaoling Wu, Zhan Su, Tianlan Li, Shanshan Liu, Yan Gao, Jiaxiu Liu, Xianqi Feng, Wei Wang, Hongguo Zhao, Zhongguang Cui, Guanglun Li, Fanjun Meng
Three dimensional (3D) culture has gradually become a research hotspot in the field of drug screening, stem cell research, and tissue engineering due to its more physiological-like morphology and function. In this study, we compared the differences of cell proliferation, population, protein expression and chemoresistance profiles between two dimensional (2D) and 3D culture of acute lymphoblastic leukemia (ALL) Jurkat cell line. Polycaprolactone (PCL) is used for 3D culture owing to its biochemical properties and compatibility...
March 2019: Experimental and Therapeutic Medicine
Huixin Zhang, Jin Wu, Jiahuan Wu, Qi Fan, Jingchao Zhou, Junwen Wu, Sichen Liu, Jie Zang, Jinhai Ye, Ming Xiao, Tian Tian, Jun Gao
BACKGROUND: Accumulating evidence shows that microRNA-210 (miR-210) holds great promise to improve angiogenesis for brain tissue repair after cerebral ischemia. However, safe and efficient delivery of miR-210 via intravenous administration is still a challenge. In the past decade, exosomes have emerged as a novel endogenous delivery system. Here, c(RGDyK) peptide is conjugated to exosomes, and they are loaded with cholesterol-modified miR-210 (RGD-exo:miR-210). RESULTS: In a transient middle cerebral artery occlusion (MCAO) mouse model, the RGD-exo:miR-210 targets the lesion region of the ischemic brain after intravenous administration, resulting in an increase in miR-210 at the site...
February 19, 2019: Journal of Nanobiotechnology
Jackelien G M van Beek, Quincy C C van den Bosch, Nicole Naus, Dion Paridaens, Annelies de Klein, Emine Kiliç, Robert M Verdijk
The aim of this study was to investigate the presence of intraocular lymphatic vessels in patients with uveal melanomas and extrascleral extension using a panel of lymphatic markers. The following immunohistochemical markers were analyzed: lymphatic vessel endothelial hyaluronic acid receptor-1 (LYVE-1), podoplanin (D2-40), prospero-related homeobox gene-1 (Prox-1), pan-endothelial marker cluster of differentiation 31 (CD31), and blood vessel endothelium-specific CD34. Lymphatic vessels were defined as a combination of staining of the following positive markers: LYVE-1, D2-40, Prox-1, and CD31; and no staining of the negative marker CD34...
February 15, 2019: Cancers
P-F Shen, B Wang, Y-X Qu, C Zheng, J-D Xu, Z-K Xie, Y Ma
OBJECTIVE: The aim of the study was to explore the role of microRNA-23c in the differentiation of marrow stromal cells (MSCs) to chondrocytes and its potential mechanism. MATERIALS AND METHODS: MSCs were first isolated from rat bone marrow for cell culture. Surface antigens of MSCs (CD29 and CD34) were identified by flow cytometry. MSCs were induced for chondrogenic differentiation in MCDM (Mesenchymal Stem Cell Chondrogenic Differentiation Medium) for 0, 3, and 7 days, respectively, followed by detection of RUNX2, microRNA-23c and FGF2 expressions by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR)...
February 2019: European Review for Medical and Pharmacological Sciences
Matías Grodzielski, Nora P Goette, Ana C Glembotsky, M Constanza Baroni Pietto, Santiago P Méndez-Huergo, Marta S Pierdominici, Verónica S Montero, Gabriel A Rabinovich, Felisa C Molinas, Paula G Heller, Paola R Lev, Rosana F Marta
Mechanisms leading to low platelet count in immune thrombocytopenia (ITP) involves both decreased production and increased destruction of platelet. However, the contribution of these pathologic mechanisms to clinical outcome of individual patients is uncertain. Here we evaluated different pathogenic mechanisms including in vitro megakaryopoiesis, platelet/megakaryocyte (MK) desialylation and MK apoptosis, and compared these effects with thrombopoyesis and platelet apoptosis in the same cohort of ITP patients...
February 18, 2019: Scientific Reports
Chien-Kuan Lee, Ken-Liao Liu, Sheng-Kai Huang
BACKGROUND: Solitary fibrous tumor (SFT) is a ubiquitous mesenchymal neoplasm but it rarely occurs in the parotid gland. The histological features are variable, with the majority having spindle cell morphology and non-specific branching (staghorn) ecstatic vascular pattern. SFT ranges from benign to overtly malignant. Dedifferentiation within SFTs represents an abrupt transition from a well-differentiated component to a high-grade area, the latter most often including poorly differentiated epithelioid/round cell or high-grade spindle cell morphology...
February 18, 2019: Diagnostic Pathology
Erik De Clercq
AMD3100 (plerixafor, Mozobil®) was first identified as an anti-HIV agent specifically active against the T4-lymphotropic HIV strains, as it selectively blocked the CXCR4 receptor. Through interference with the interaction of CXCR4 with its natural ligand, SDF-1 (also named CXCL12), it also mobilized the CD34+ stem cells from the bone marrow into the peripheral blood stream. In December 2008, AMD3100 was formally approved by the US FDA for autologous transplantation in patients with Non-Hodgkin's Lymphoma or multiple myeloma...
January 2019: Antiviral Chemistry & Chemotherapy
Rohit P Nagare, S Sneha, S Ramesh, Trivadi S Ganesan
Stem cells or Cancer stem cells (CSCs) have now been identified in different type of tissues by using surface markers. Functional assays such as ALDEFLUOR and side population which are marker independent have been additional approaches. However, whether all these approaches identify the same population of cells remain uncertain. To address this issue we have used hematopoietic stem cells as a model. Peripheral blood stem cells enumerated by CD34 are used routinely in bone marrow transplantation which supports the recovery of bone marrow after ablative chemotherapy or radiation...
February 15, 2019: International Journal of Biochemistry & Cell Biology
Deliang Shen, Zhenhua Li, Shiqi Hu, Ke Huang, Teng Su, Hongxia Liang, Feiran Liu, Ke Cheng
Stem cell therapies have shown promise in treating acute and chronic ischemic heart disease. However, current therapies are limited by the low retention and poor integration of injected cells in the injured tissue. Taking advantage of the natural infarct-homing ability of platelets, we engineered CD34 antibody-linked platelets (P-CD34) to capture circulating CD34-positive endogenous stem cells and direct them to the injured heart. In vitro, P-CD34 could bind to damaged aortas and capture endogenous stem cells in whole blood...
February 18, 2019: Nano Letters
Prita Pradhan, Biswajit Dey, Bheemanathi Hanuman Srinivas, Sajini Elizabeth Jacob, Roopesh Kumar Vadivel Rathakrishnan
Background: Pleomorphic xanthoastrocytoma is a rare tumour of children and young adults, particularly for those with features of anaplasia. Materials and Methods : This retrospective study comprises five cases of anaplastic pleomorphic xanthoastrocytomas diagnosed over a period of 4 years in a tertiary care institute. A detailed clinicopathological and immunohistochemical profile of the tumours were noted from the hospital database. Results: Five cases of anaplastic pleomorphic xanthoastrocytomas were evaluated for their clinicoradiological, histomorphological as well as immunohistochemical findings, which included 3 females and 2 males, with age range of 11-40 years and a mean age at presentation of 22 years...
October 1, 2018: International Journal of Hematology-oncology and Stem Cell Research
Paulina Gil-Kulik, Arkadiusz Krzyżanowski, Ewa Dudzińska, Jolanta Karwat, Piotr Chomik, Małgorzata Świstowska, Adrianna Kondracka, Anna Kwaśniewska, Maria Cioch, Mariusz Jojczuk, Adam Nogalski, Janusz Kocki
The BIRC5 gene encodes a survivin protein belonging to class III of inhibitors of apoptosis, IAP. This protein serves a dual role. First, it regulates cell death, and second, it is an important regulator of mitosis progression, although its physiological regulatory function has not been fully understood. Many studies have shown and confirmed that survivin is practically absent in mature tissues in nature, while its overexpression has been reported in many cancerous tissues. There is little information about the significance of BIRC5 expression in normal adult human stem cells...
2019: Oxidative Medicine and Cellular Longevity
Turan Yaman, Abdullah Karasu, Ahmet Uyar, Yağmur Kuşçu, Ömer Faruk Keleş
A 1-mo-old Ivesi male lamb was presented with 2 large red masses on the skin of the left ear. The tumors were removed using gentle dissection and submitted for histologic evaluation. The tumors consisted of numerous thin-walled capillaries lined by endothelial cells and nests of stromal cells. Immunohistochemically, the endothelial cells were positive for CD45, and the stromal cells were positive for neuron-specific enolase. GFAP-positive cells were occasionally present within the tumor. Endothelial and stromal cells were negative for S100, CD34, CD31, and factor VIII-related antigen...
February 17, 2019: Journal of Veterinary Diagnostic Investigation
Dan Mi, De-Cheng Wang, Chong Pan
OBJECTIVE: To observe the effect of eye-acupuncture intervention on cerebro-cortical apoptosis of microvascular endothelial cells, neurons and astrocytes (main components of neurovascular unit) and the expression of Bad (an apoptosis promoter) and B-cell lymphoma-extra large(Bcl-xL) proteins in focal cerebral ischemia-reperfusion injury (CIRI) rats, so as to explore its mechanisms underlying improvement of CIRI. METHODS: SD rats were randomly divided into control, sham-operation, model 3 h, model 24 h, model 72 h, eye-acupuncture 3 h, eye-acupuncture 24 h and eye-acupuncture 72 h groups( n =12 in each group)...
January 25, 2019: Zhen Ci Yan Jiu, Acupuncture Research
Alexandra Haase, Wolfgang Glienke, Lena Engels, Gudrun Göhring, Ruth Esser, Lubomir Arseniev, Ulrich Martin
The utilization of human induced pluripotent stem cells (hiPSCs) for disease modeling and drug discovery is already reality, and several first-in-man-applications as cellular therapeutics have been initiated. Implementation of good manufacturing practice (GMP)-compliant protocols for the generation of hiPSC lines is crucial to increase the application safety as well as to fulfil the legal requirements for clinical trials approval. Here we describe the development of a GMP-compatible protocol for the reprogramming of CD34+ hematopoietic stem cells from peripheral blood (CD34+ PBHSC) into hiPSCs using Sendai virus-based reprogramming vectors...
February 11, 2019: Stem Cell Research
Huifeng Yue, Xiaotong Ji, Yingying Zhang, Guangke Li, Nan Sang
The placenta is the bridge that connects the developing fetus and mother and is the place where nutrient uptake, waste elimination and gas exchange occurs. Epidemiological studies indicate that fetal stress, such as gestational PM2.5 (particulate matter (PM) with diameter ≤ 2.5 μm) exposure, is a risk factor for abnormal placenta development that could induce adverse effects on the fetus. In the present study, PM samples were collected in Taiyuan. Then, pregnant C57BL/6 mice were treated with 3 mg/kg b...
February 7, 2019: Science of the Total Environment
Rintaro Ono, Takashi Watanabe, Eiryo Kawakami, Makoto Iwasaki, Mariko Tomizawa-Murasawa, Masashi Matsuda, Yuho Najima, Shinsuke Takagi, Saera Fujiki, Rumi Sato, Yoshiki Mochizuki, Hisahiro Yoshida, Kaoru Sato, Hiromasa Yabe, Shunichi Kato, Yoriko Saito, Shuichi Taniguchi, Leonard D Shultz, Osamu Ohara, Masayuki Amagai, Haruhiko Koseki, Fumihiko Ishikawa
BACKGROUND: Graft-versus host disease (GVHD) is a complication of stem cell transplantation associated with significant morbidity and mortality. Non-specific immune-suppression, the mainstay of treatment, may result in immune-surveillance dysfunction and disease recurrence. METHODS: We created humanised mice model for chronic GVHD (cGVHD) by injecting cord blood (CB)-derived human CD34+ CD38- CD45RA- haematopoietic stem/progenitor cells (HSPCs) into hIL-6 transgenic NOD/SCID/Il2rgKO (NSG) newborns, and compared GVHD progression with NSG newborns receiving CB CD34- cells mimicking acute GVHD...
February 13, 2019: EBioMedicine
Sophia Fernandes, Shruti Tembe, Sanjay Singh, Shakti Vardhan, Velu Nair, Vaijayanti Kale, Lalita Limaye
No abstract text is available yet for this article.
February 13, 2019: Stem Cell Research
Lin Guo, Lihui Lin, Xiaoshan Wang, Mingwei Gao, Shangtao Cao, Yuanbang Mai, Fang Wu, Junqi Kuang, He Liu, Jiaqi Yang, Shilong Chu, Hong Song, Dongwei Li, Yujian Liu, Kaixin Wu, Jiadong Liu, Jinyong Wang, Guangjin Pan, Andrew P Hutchins, Jing Liu, Duanqing Pei, Jiekai Chen
Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs), which is a highly heterogeneous process. Here we report the cell fate continuum during somatic cell reprogramming at single-cell resolution. We first develop SOT to analyze cell fate continuum from Oct4/Sox2/Klf4- or OSK-mediated reprogramming and show that cells bifurcate into two categories, reprogramming potential (RP) or non-reprogramming (NR). We further show that Klf4 contributes to Cd34+/Fxyd5+/Psca+ keratinocyte-like NR fate and that IFN-γ impedes the final transition to chimera-competent pluripotency along the RP cells...
February 13, 2019: Molecular Cell
Rehan M Hussain, Sander R Dubovy, Ajay E Kuriyan, Xiao Yi Zhou, Harry W Flynn, Thomas A Albini
The histologic findings of a subretinal band and epiretinal membrane (ERM) excised from two patients who developed retinal detachments (RDs) after non-U.S. Food and Drug Administration-regulated intravitreal "stem cell" injections are reported. Both membranes were composed of fibrocellular tissue that stained positively with Smooth Muscle Actin and Masson's trichrome, consistent with collagenous and smooth muscle composition. CD34 immunostain (for hematopoietic cells) was negative for the subretinal band and minimally positive for the ERM...
February 1, 2019: Ophthalmic Surgery, Lasers & Imaging Retina
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