keyword
https://read.qxmd.com/read/38293239/multiple-pathways-impact-swarming-motility-of-pseudomonas-fluorescens-pf0-1
#1
Alexander B Pastora, Kara M Rzasa, George A O'Toole
UNLABELLED: Swarming motility in pseudomonads typically requires both a functional flagellum and production/secretion of a biosurfactant. Published work has shown that the wild-type Pseudomonas fluorescens Pf0-1 is swarming-deficient due to a point mutation in the gacA gene, which until recently, was thought to inactivate rather than attenuate the Gac/Rsm pathway. As a result, little is known about the underlying mechanisms that regulate swarming motility by P. fluorescens Pf0-1. Here, we demonstrate that a Δ rsmA Δ rsmE Δ rsmI mutant, which phenotypically mimics Gac/Rsm pathway overstimulation, is proficient at swarming motility...
January 18, 2024: bioRxiv
https://read.qxmd.com/read/38176122/self-determined-sequence-exercise-program-for-elderly-with-sarcopenia-a-randomized-controlled-trial-with-clinical-assistance-from-explainable-artificial-intelligence
#2
JOURNAL ARTICLE
Shichun He, Meiqi Wei, Deyu Meng, Ziyi Wang, Guang Yang, Ziheng Wang
To improve and even reverse sarcopenia in elderly people, this study developed a self-determined sequence exercise program consisting of strength training exercise, Yijinjing exercise (a traditional Chinese exercise), and hybrid strength training with Yijinjing exercise. Ninety-four community-dwelling older adults screened for sarcopenia using the Asian Working Group for Sarcopenia criteria were randomly assigned to 24 weeks of a control group (CG, n = 30), self-determined sequence exercise program group (SDSG, n = 34) or strength training group (STG, n = 30)...
December 21, 2023: Archives of Gerontology and Geriatrics
https://read.qxmd.com/read/38046438/the-diagnostic-cut-off-points-for-components-of-sarcopenia-in-finnish-caucasian-women-a-retrospective-cross-sectional-study
#3
JOURNAL ARTICLE
Samu Sjöblom, Juha Suuronen, Toni Rikkonen, Risto Honkanen, Heikki Kröger, Joonas Sirola
OBJECTIVES: To determine the diagnostic cut-off values of components for sarcopenia in Caucasian women. METHODS: The present retrospective cross-sectional study based on the REFERENCE sample included 400 healthy women aged 20 to 40 years, and the OSTPRE sample included 344 women aged 63 to 75. The subjects of the OSTPRE population were re-measured five and ten years later after the baseline. Both samples underwent grip strength (GS), quadriceps strength (QS), and total-body DXA (TB-DXA) measurements, from which Relative Skeletal Muscle Mass Index (RSMI) was calculated...
December 2023: Journal of frailty, sarcopenia and falls
https://read.qxmd.com/read/36759518/the-natural-pyrazolotriazine-pseudoiodinine-from-pseudomonas-mosselii-923-inhibits-plant-bacterial-and-fungal-pathogens
#4
JOURNAL ARTICLE
Ruihuan Yang, Qing Shi, Tingting Huang, Yichao Yan, Shengzhang Li, Yuan Fang, Ying Li, Linlin Liu, Longyu Liu, Xiaozheng Wang, Yongzheng Peng, Jiangbo Fan, Lifang Zou, Shuangjun Lin, Gongyou Chen
Natural products largely produced by Pseudomonads-like soil-dwelling microorganisms are a consistent source of antimicrobial metabolites and pesticides. Herein we report the isolation of Pseudomonas mosselii strain 923 from rice rhizosphere soils of paddy fields, which specifically inhibit the growth of plant bacterial pathogens Xanthomonas species and the fungal pathogen Magnaporthe oryzae. The antimicrobial compound is purified and identified as pseudoiodinine using high-resolution mass spectra, nuclear magnetic resonance and single-crystal X-ray diffraction...
February 9, 2023: Nature Communications
https://read.qxmd.com/read/36469533/trna-modification-enzyme-miab-connects-environmental-cues-to-activation-of-pseudomonas-aeruginosa-type-iii-secretion-system
#5
JOURNAL ARTICLE
Qiqi Lin, Jiahui Huang, Zhiqing Liu, Qunyi Chen, Xinbo Wang, Guohui Yu, Ping Cheng, Lian-Hui Zhang, Zeling Xu
Pseudomonas aeruginosa, a major inhabitant of numerous environmental reservoirs, is a momentous opportunistic human pathogen associated with severe infections even death in the patients suffering from immune deficiencies or metabolic diseases. Type III secretion system (T3SS) employed by P. aeruginosa to inject effector proteins into host cells is one of the pivotal virulence factors pertaining to acute infections caused by this pathogen. Previous studies showed that P. aeruginosa T3SS is activated by various environmental cues such as calcium concentration and the host signal spermidine...
December 5, 2022: PLoS Pathogens
https://read.qxmd.com/read/36228460/sil-net-a-semi-isotropic-l-shaped-network-for-dermoscopic-image-segmentation
#6
JOURNAL ARTICLE
Zequn Zhang, Yun Jiang, Hao Qiao, Meiqi Wang, Wei Yan, Jie Chen
BACKGROUND: Dermoscopic image segmentation using deep learning algorithms is a critical technology for skin cancer detection and therapy. Specifically, this technology is a spatially equivariant task and relies heavily on Convolutional Neural Networks (CNNs), which lost more effective features during cascading down-sampling or up-sampling. Recently, vision isotropic architecture has emerged to eliminate cascade procedures in CNNs as well as demonstrates superior performance. Nevertheless, it cannot be used for the segmentation task directly...
September 27, 2022: Computers in Biology and Medicine
https://read.qxmd.com/read/36165622/spatial-structure-formation-by-rsme-regulated-extracellular-secretions-in-pseudomonas-fluorescens-pf0-1
#7
JOURNAL ARTICLE
Anton F Evans, Meghan K Wells, Jordan Denk, William Mazza, Raziel Santos, Amber Delprince, Wook Kim
Cells in microbial communities on surfaces live and divide in close proximity, which greatly enhances the potential for social interactions. Spatiogenetic structures are manifested through competitive and cooperative interactions among the same and different genotypes within a shared space, and extracellular secretions appear to function dynamically at the forefront. A previous experimental evolution study utilizing Pseudomonas fluorescens Pf0-1 colonies demonstrated that diverse mutations in the rsmE gene were repeatedly and exclusively selected through the formation of a dominant spatial structure...
September 27, 2022: Journal of Bacteriology
https://read.qxmd.com/read/36011588/hybrid-exercise-program-for-sarcopenia-in-older-adults-the-effectiveness-of-explainable-artificial-intelligence-based-clinical-assistance-in-assessing-skeletal-muscle-area
#8
RANDOMIZED CONTROLLED TRIAL
Meiqi Wei, Deyu Meng, Hongzhi Guo, Shichun He, Zhibo Tian, Ziyi Wang, Guang Yang, Ziheng Wang
BACKGROUND: Sarcopenia is a geriatric syndrome characterized by decreased skeletal muscle mass and function with age. It is well-established that resistance exercise and Yi Jin Jing improve the skeletal muscle mass of older adults with sarcopenia. Accordingly, we designed an exercise program incorporating resistance exercise and Yi Jin Jing to increase skeletal muscle mass and reverse sarcopenia in older adults. Additionally, machine learning simulations were used to predict the sarcopenia status after the intervention...
August 12, 2022: International Journal of Environmental Research and Public Health
https://read.qxmd.com/read/35837862/rsma3-modulates-rpos-through-the-rets-gac-rsm-signalling-pathway-in-response-to-h-2-o-2-stress-in-the-phytopathogen-pseudomonas-syringae
#9
JOURNAL ARTICLE
Xun Yu, Cui Meng, Xiaocheng Tan, Yuwei Su, Zhiping Cao, Tzann-Shun Hwang, Lin Li
Reactive oxygen species are a fatal challenge to the plant pathogenic bacterium Pseudomonas syringae. In this study, we reveal that the global regulatory protein RsmA3 from the RetS-Gac/Rsm signalling pathway modulates RpoS in the early-log growth phase in the P. syringae wild-type strain MB03, thereby regulating oxidative tolerance to H2 O2 and ultimately affecting pathogenicity to the host plant. Following increased H2 O2 by external addition or endogenous induction by menadione, the resistance of the mutant strain ΔretS to H2 O2 is significantly enhanced due to rapid increases in the transcription of Rsm-related non-coding small RNAs (nc sRNAs), a sigma factor RpoS, and H2 O2 -detoxifying enzymes...
July 15, 2022: Environmental Microbiology
https://read.qxmd.com/read/35800768/interspecies-effectors-of-a-transgenerational-memory-of-bacterial-infection-in-caenorhabditis-elegans
#10
JOURNAL ARTICLE
Marcela Legüe, Mauricio Caneo, Blanca Aguila, Bernardo Pollak, Andrea Calixto
The inheritance of memory is an adaptive trait. Microbes challenge the immunity of organisms and trigger behavioral adaptations that can be inherited, but how bacteria produce inheritance of a trait is unknown. We use Caenorhabditis elegans and its bacteria to study the transgenerational RNA dynamics of interspecies crosstalk leading to a heritable behavior. A heritable response of C. elegans to microbes is the pathogen-induced diapause (PIDF), a state of suspended animation to evade infection. We identify RsmY, a small RNA involved in quorum sensing in Pseudomonas aeruginosa as a trigger of PIDF...
July 15, 2022: IScience
https://read.qxmd.com/read/35348363/rets-regulates-phage-infection-in-pseudomonas-aeruginosa-via-modulating-the-gacs-gaca-two-component-system
#11
JOURNAL ARTICLE
Guanhua Xuan, Hong Lin, Xiaoyan Li, Jiuna Kong, Jingxue Wang
In Pseudomonas aeruginosa, the complex multisensing regulatory networks RetS-GacS/GacA have been demonstrated to play key roles in controlling the switch between planktonic and sessile lifestyles. However, whether this multisensing system is involved in the regulation of phage infection has not been investigated. Here, we provide a link between the sensors RetS/GacS and infection of phages vB_Pae_QDWS and vB_Pae_W3. Our data suggest that the sensors kinases RetS and GacS in Pseudomonas aeruginosa play opposite regulatory functions on phage infection...
March 29, 2022: Journal of Virology
https://read.qxmd.com/read/35336241/dihydrolipoamide-acetyltransferase-acef-influences-the-type-iii-secretion-system-and-resistance-to-oxidative-stresses-through-rsmy-z-in-pseudomonas-aeruginosa
#12
JOURNAL ARTICLE
Haozhou Li, Yushan Xia, Zhenyang Tian, Yongxin Jin, Fang Bai, Zhihui Cheng, Wieslaw Swietnicki, Weihui Wu, Xiaolei Pan
Carbon metabolism plays an important role in bacterial physiology and pathogenesis. The type III secretion system (T3SS) of Pseudomonas aeruginosa is a virulence factor that contributes to acute infections. It has been demonstrated that bacterial metabolism affects the T3SS. Meanwhile, expression of T3SS genes is negatively regulated by the small RNAs RsmY and RsmZ. In this study, we studied the relationship between the dihydrolipoamide acetyltransferase gene aceF and the T3SS. Our results reveal an upregulation of RsmY and RsmZ in the aceF mutant, which represses the expression of the T3SS genes...
March 21, 2022: Microorganisms
https://read.qxmd.com/read/35140216/translocated-legionella-pneumophila-small-rnas-mimic-eukaryotic-micrornas-targeting-the-host-immune-response
#13
JOURNAL ARTICLE
Tobias Sahr, Pedro Escoll, Christophe Rusniok, Sheryl Bui, Gérard Pehau-Arnaudet, Gregory Lavieu, Carmen Buchrieser
Legionella pneumophila is an intracellular bacterial pathogen that can cause a severe form of pneumonia in humans, a phenotype evolved through interactions with aquatic protozoa in the environment. Here, we show that L. pneumophila uses extracellular vesicles to translocate bacterial small RNAs (sRNAs) into host cells that act on host defence signalling pathways. The bacterial sRNA RsmY binds to the UTR of ddx58 (RIG-I encoding gene) and cRel, while tRNA-Phe binds ddx58 and irak1 collectively reducing expression of RIG-I, IRAK1 and cRel, with subsequent downregulation of IFN-β...
February 9, 2022: Nature Communications
https://read.qxmd.com/read/35104679/elevated-expression-of-rsmi-can-act-as-a-reporter-of-aminoglycoside-resistance-in-escherichia-coli-using-kanamycin-as-signal-molecule
#14
JOURNAL ARTICLE
Jayalaxmi Wangkheimayum, Deepjyoti Paul, Debadatta Dhar Chanda, K Melson Singha, Amitabha Bhattacharjee
We aimed to design and analyse expressional response of endogenous and exogenous 16S rRNA methyl transferase genes under sub inhibitory concentration stress of different clinically relevant aminoglycoside antibiotics in Escherichia coli to identify an endogenous marker. One hundred twenty nine aminoglycoside resistant E. coli of clinical origin were collected for detection of 16S rRNA methyl transferase genes by PCR assay and each gene type was cloned within E. coli JM107. Parent isolates were subjected to plasmid elimination by SDS treatment...
January 29, 2022: Infection, Genetics and Evolution
https://read.qxmd.com/read/35041497/direct-inhibition-of-rets-synthesis-by-rsma-contributes-to-homeostasis-of-the-pseudomonas-aeruginosa-gac-rsm-signaling-system
#15
JOURNAL ARTICLE
Jodi M Corley, Peter Intile, Timothy L Yahr
The Gac/Rsm system is a global regulator of Pseudomonas aeruginosa gene expression. The primary effectors are RsmA and RsmF. Both are RNA-binding proteins that interact with target mRNAs to modulate protein synthesis. RsmA/RsmF recognize GGA sequences presented in the loop portion of stem-loop structures. For repressed targets, the GGA sites usually overlap the ribosome binding site (RBS) and RsmA/RsmF binding inhibits translation initiation. RsmA/RsmF activity is controlled by several small non-coding RNAs (sRNA) that sequester RsmA/RsmF from target mRNAs...
January 18, 2022: Journal of Bacteriology
https://read.qxmd.com/read/35030903/symmetries-and-phase-diagrams-with-real-space-mutual-information-neural-estimation
#16
JOURNAL ARTICLE
Doruk Efe Gökmen, Zohar Ringel, Sebastian D Huber, Maciej Koch-Janusz
Real-space mutual information (RSMI) was shown to be an important quantity, formally and from a numerical standpoint, in finding coarse-grained descriptions of physical systems. It very generally quantifies spatial correlations and can give rise to constructive algorithms extracting relevant degrees of freedom. Efficient and reliable estimation or maximization of RSMI is, however, numerically challenging. A recent breakthrough in theoretical machine learning has been the introduction of variational lower bounds for mutual information, parametrized by neural networks...
December 2021: Physical Review. E
https://read.qxmd.com/read/34557608/prevalence-and-factors-associated-with-recurrent-falls-among-middle-aged-community-dwelling-women
#17
JOURNAL ARTICLE
Nirmala Rathnayake, Sarath Lekamwasam
Objective: This community-based study evaluated the prevalence and associated risk factors of recurrent falls among middle-aged community-dwelling women in Southern-Sri Lanka. Methods: Randomly selected 285 middle-aged women (40-60years, Mean±SD;51.7±6.1years) participated. History of falls within the previous 12-months was inquired and those who reported two or more falls within 6-month period were considered as recurrent fallers. Age, menopausal status, weight (kg), height (m), waist-circumference (WC, cm), appendicular-skeletal-muscle-mass (ASMM, kg by DXA), hand-grip-strength (HGS, kg) and gait-speed (GS, m/s) were evaluated...
September 2021: Journal of frailty, sarcopenia and falls
https://read.qxmd.com/read/34478825/changes-in-body-weight-and-metabolic-risk-during-time-restricted-feeding-in-premenopausal-versus-postmenopausal-women
#18
JOURNAL ARTICLE
Sofia Cienfuegos, Kelsey Gabel, Faiza Kalam, Mark Ezpeleta, Shuhao Lin, Krista A Varady
BACKGROUND: Time restricted feeding (TRF) involves confining the eating window to a specific number of hours, and fasting for the remaining hours of the day. OBJECTIVE: This study examined if changes in body weight and metabolic risk factors during TRF, differ between premenopausal and postmenopausal women. METHODS: This is a secondary analysis of an 8-week TRF study (4-6 h eating window, 18-20 h fasting window daily) conducted in adults with obesity...
October 15, 2021: Experimental Gerontology
https://read.qxmd.com/read/34311171/zhuanggu-zhitong-capsule-alleviates-osteosarcopenia-in-rats-by-up-regulating-pi3k-akt-bcl2-signaling-pathway
#19
JOURNAL ARTICLE
Jiangtao Ma, Maolin Ye, Ying Li, Shuang Chai, Hong Huang, Xiaohang Lian, Hongxing Huang
BACKGROUND AND AIMS: Osteosarcopenia (OS), characterized by the coexistence of osteoporosis (OP) and sarcopenia (SP), is associated with high morbidity and mortality in the elderly. Nevertheless, its pathogenesis and treatment remain unclear. The aim of this study was to investigate the effect and mechanism of Zhuanggu Zhitong Capsule (ZGZT) in OS rats. METHODS: All the related targets of OS, corresponding targets for bioactive ingredients of ZGZT, intersection targets of ZGZT against OS, and signaling pathways were predicted and analyzed by network pharmacology...
October 2021: Biomedicine & Pharmacotherapy
https://read.qxmd.com/read/34095158/osteosarcopenia-in-very-old-age-adults-after-hip-fracture-a-real-world-therapeutic-standpoint
#20
JOURNAL ARTICLE
Monica Pizzonia, Andrea Casabella, Marta Natali, Lorena Petrocchi, Luca Carmisciano, Alessio Nencioni, Luigi Molfetta, Chiara Giannotti, Gerolamo Bianchi, Andrea Giusti, Federico Santolini, Fiammetta Monacelli
Loss of bone and muscle mass and strength (i. e., osteosarcopenia) is a highly prevalent clinical condition in older adults, associated with an increased risk of fragility fractures and unfavorable clinical outcomes. Although sarcopenia is a potential risk factor for osteoporosis and subsequent fracture, and the management of this hazardous duet is the key to preventing osteoporotic fracture, evidence pertaining to the treatment of sarcopenia for the purpose of preventing fragile fractures remains insufficient...
2021: Frontiers in Medicine
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