Megan W Tse, Meilin Zhu, Benjamin Peters, Efrat Hamami, Julie Chen, Kathleen P Davis, Samuel Nitz, Juliane Weller, Thulasi Warrier, Diana K Hunt, Yoelkys Morales, Tomohiko Kawate, Jeffrey L Gaulin, Jon H Come, Juan Hernandez-Bird, Wenwen Huo, Isabelle Neisewander, Laura L Kiessling, Deborah T Hung, Joan Mecsas, Bree B Aldridge, Ralph R Isberg, Paul C Blainey
UNLABELLED: Antibiotic resistance, especially in multidrug-resistant ESKAPE pathogens, remains a worldwide problem. Combination antimicrobial therapies may be an important strategy to overcome resistance and broaden the spectrum of existing antibiotics. However, this strategy is limited by the ability to efficiently screen large combinatorial chemical spaces. Here, we deployed a high-throughput combinatorial screening platform, DropArray, to evaluate the interactions of over 30,000 compounds with up to 22 antibiotics and 6 strains of Gram-negative ESKAPE pathogens, totaling to over 1...
March 26, 2024: bioRxiv