Arkaitz Ibarra, Jiali Zhuang, Yue Zhao, Neeraj S Salathia, Vera Huang, Alexander D Acosta, Jonathan Aballi, Shusuke Toden, Amy P Karns, Intan Purnajo, Julianna R Parks, Lucy Guo, James Mason, Darren Sigal, Tina S Nova, Stephen R Quake, Michael Nerenberg
Circulating cell-free mRNA (cf-mRNA) holds great promise as a non-invasive diagnostic biomarker. However, cf-mRNA composition and its potential clinical applications remain largely unexplored. Here we show, using Next Generation Sequencing-based profiling, that cf-mRNA is enriched in transcripts derived from the bone marrow compared to circulating cells. Further, longitudinal studies involving bone marrow ablation followed by hematopoietic stem cell transplantation in multiple myeloma and acute myeloid leukemia patients indicate that cf-mRNA levels reflect the transcriptional activity of bone marrow-resident hematopoietic lineages during bone marrow reconstitution...
January 21, 2020: Nature Communications