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Mobilized Stem Cell

Ting Guo, Zi Nan, Chen Miao, Xiaoye Jin, Weiwei Yang, Zehua Wang, Yinqi Tu, Hongcun Bao, Jialan Lyu, Huimei Zheng, Qiannan Deng, Pengfei Guo, Yongmei Xi, Xiaohang Yang, Wanzhong Ge
Atg101 is an autophagy-related gene identified in worms, flies, mice, and mammals and encodes a protein that functions in autophagosome formation by associating with the ULK1-Atg13-Fip200 complex. In the last few years, the critical role of Atg101 in autophagy has been well established through biochemical studies and the determination of its protein structure. However, Atg101's physiological role both during development and in adulthood remains less understood. Here, we describe the generation and characterization of an Atg101 loss-of-function mutant in Drosophila and report on the roles of Atg101 in maintaining tissue homeostasis in both adult brains and midguts...
February 13, 2019: Journal of Biological Chemistry
Li Chen, Kaikai Shi, Thomas Levin Andersen, Weimin Qiu, Moustapha Kassem
Factors mediating mobilization of osteoblastic stem and progenitor cells from their bone marrow niche to be recruited to bone formation sites during bone remodeling are poorly known. We have studied secreted factors present in the bone marrow microenvironment and identified KIAA1199 (also known as CEMIP, cell migration inducing hyaluronan binding protein) in human bone biopsies as highly expressed in osteoprogenitor reversal cells (Rv.C) recruited to the eroded surfaces (ES), which are the future bone formation sites...
February 12, 2019: Cell Death & Disease
Hong Xu, Ziqiang Zhu, Yiming Huang, Suzanne T Ildstad
BACKGROUND: Mobilization of hematopoietic stem cells (HSC) has become the preferred approach for HSC transplantation. AMD3100, a competitive inhibitor of C-X-C motif chemokine receptor-4 (CXCR4), has been found to be a rapid mobilizing agent. The present study evaluated approaches to optimize the product collected. METHODS: Mobilized peripheral blood mononuclear cells (mPBMC) from B6 mice were transplanted to recipient BALB/c mice conditioned with ablative or nonmyeloablative approaches...
February 4, 2019: Transplantation
Weihua Ren, Jianfeng Hou, Chenguang Yang, Hanjun Wang, Shuangting Wu, Yabin Wu, Xingpeng Zhao, Chao Lu
OBJECTIVE: To investigate the lung cancer-promoting mechanism of mesenchymal stem cell-secreted extracellular vesicles (MSC-EV). METHODS: EV were isolated from culture media of human bone marrow-derived MSCs that were pre-challenged with or without hypoxia (referred to as H-EV and N-EV, respectively). After treatment with N-EV or H-EV, A549 and H23 cell proliferation, apoptosis, trans-well invasion and epithelial-to-mesenchymal transition (EMT) were examined. Polarization of human primary monocytes-derived macrophages with or without N-EV or H-EV induction were analyzed by flow cytometry and ELISA...
February 8, 2019: Journal of Experimental & Clinical Cancer Research: CR
Xiao-Tao He, Xuan Li, Yu Xia, Yuan Yin, Rui-Xin Wu, Hai-Hua Sun, Fa-Ming Chen
Recently, we found that although high-stiffness matrices stimulated osteogenic differentiation of bone marrow-derived stromal cells (BMSCs), the macrophages (Mφs) in high-stiffness transglutaminase crosslinked gelatins (TG-gels) tended to undergo M1 polarization and hence compromised cell osteogenesis. In this study, we hypothesized that the copresentation of interleukin (IL)-4 and stromal cell-derived factor (SDF)-1α in high-stiffness TG-gels may enhance periodontal regeneration by modulating Mφ polarization and promoting endogenous stem cell recruitment...
February 5, 2019: Acta Biomaterialia
Kavita Bisht, Marion E Brunck, Taichi Matsumoto, Crystal McGirr, Bianca Nowlan, Whitney Fleming, Thomas Keech, Graham Magor, Andrew C Perkins, Julie Davies, Gail Walkinshaw, Lee Flippin, Ingrid G Winkler, Jean-Pierre Levesque
In normoxia, hypoxia-inducible transcription factors (HIFs) are rapidly degraded within the cytoplasm as a consequence of their prolyl hydroxylation by oxygen-dependent prolyl hydroxylase domain (PHD) enzymes. We have previously shown that hematopoietic stem and progenitor cells (HSPCs) require HIF-1 for effective mobilization in response to granulocyte colony-stimulating factor (G-CSF) and CXCR4 antagonist AMD3100/plerixafor. Conversely, HIF PHD inhibitors that stabilize HIF-1 protein in vivo enhance HSPC mobilization in response to G-CSF or AMD3100 in a cell-intrinsic manner...
February 12, 2019: Blood Advances
Antonio Casado-Díaz, Gabriel Dorado, Mercè Giner, María José Montoya, Cristina Navarro-Valverde, Adolfo Díez-Pérez, José Manuel Quesada-Gómez
Osteoporosis long-term treatment with nitrogen-containing bisphosphonates, has been associated with uncommon adverse effects, as atypical femoral fractures (AFF). Thus, treatment with teriparatide (TPTD; fragment of human parathyroid hormone; PTH1-34 ) has been proposed for such patients. Besides its anabolizing effect on bone, TPTD may affect stem-cell mobilization and expansion. Bone marrow mononuclear cells (BMMNC) were isolated from five women that had suffered AFF associated to bisphosphonate treatment, before and after 6 months of TPTD therapy...
February 6, 2019: Calcified Tissue International
Richard Harrop, Eric O'Neill, Peter L Stern
Cancer stem cells (CSCs) can act as the cellular drivers of tumors harnessing stem cell properties that contribute to tumorigenesis either as founder elements or by the gain of stem cell traits by the malignant cells. Thus, CSCs can self-renew and generate the cellular heterogeneity of tumors including a hierarchical organization similar to the normal tissue. While the principle tumor growth contribution is often from the non-CSC components, it is the ability of small numbers of CSCs to avoid the effects of therapeutic strategies that can contribute to recurrence after treatment...
2019: Therapeutic advances in vaccines and immunotherapy
Nicole L Prokopishyn, Brent R Logan, Deidre M Kiefer, Jennifer A Sees, Pintip Chitphakdithai, Ibrahim A Ahmed, Paolo N Anderlini, Amer M Beitinjaneh, Christopher Bredeson, Jan Cerny, Saurabh Chhabra, Andrew Daly, Miguel Angel Diaz, Nosha Farhadfar, Haydar A Frangoul, Siddhartha Ganguly, Dennis A Gastineau, Usama Gergis, Gregory A Hale, Peiman Hematti, Rammurti T Kamble, Kimberly A Kasow, Hillard M Lazarus, Jane L Liesveld, Hemant S Murthy, Maxim Norkin, Richard F Olsson, Mona Papari, Bipin N Savani, Jeffrey Szer, Edmund K Waller, Baldeep Wirk, Jean A Yared, Michael A Pulsipher, Nirali N Shah, Galen E Switzer, Paul V O'Donnell, Dennis L Confer, Bronwen E Shaw
Bone Marrow (BM) is an essential hematopoietic stem cell (HSC) source for many allogeneic hematopoietic cell transplant (HCT) recipients, including adult patients (for specific diseases and transplant strategies) and the majority of pediatric transplants. However, since the advent of Granulocyte-colony stimulating factor (G-CSF) mobilized peripheral blood stem cells (PBSC), there has been a significant decrease in utilization of BM in transplant patients, predominantly thought to be due to the increased logistical challenges around BM harvesting compared to PBSC, and generally no significant survival advantage of BM or PBSC...
February 1, 2019: Biology of Blood and Marrow Transplantation
Yu Cai, Liping Wan, Juan Yang, Jun Zhu, Jieling Jiang, Su Li, Xianmin Song, Chun Wang
To evaluate the strategy of using high-dose etoposide mobilization followed by autologous peripheral blood stem cell transplantation (APBSCT) in patients with diffuse large B cell lymphoma (DLBCL) refractory to rituximab-based chemotherapy. Forty patients with refractory DLBCL were treated with high-dose etoposide for stem cell mobilization. All patients were in progressive disease (PD) prior to mobilization and underwent high-dose chemotherapy followed by APBSCT. Successful PBSC mobilization was achieved in all patients...
February 4, 2019: Annals of Hematology
Monika Golab-Janowska, Edyta Paczkowska, Boguslaw Machalinski, Dariusz Kotlega, Agnieszka Meller, Przemyslaw Nowacki, Safranow Krzysztof, Wankowlcz Pawel
BACKGROUND: Endothelial progenitor cells (EPCs) are important players in neovascularization, mobilized through signalling by angiogenic growth factors (AGFs) such as vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF). In vitro, inflammatory parameters impair the function and influence of EPCs on AGFs. However, this connection is not clear in vivo. To understand the mechanisms of augmented arteriogenesis and angiogenesis in acute ischemic stroke (AIS) patients, we investigated whether circulating stem cells (CD133+), early endothelial progenitor cells (CD133+/VEGFR2+), and endothelial cells (ECs; CD34¯/CD133¯/VEGFR2+) were increasingly mobilized during AIS, and whether there were correlations between EPC levels, growth factor levels and inflammatory parameters...
January 29, 2019: Current Neurovascular Research
Qilin Gu, Xiaojie Yang, Jie Lv, Jiaxiong Zhang, Bo Xia, Jun-Dae Kim, Ruoyu Wang, Feng Xiong, Shu Meng, Thomas P Clements, Bhavna Tandon, Daniel S Wagner, Miguel F Diaz, Pamela L Wenzel, Yury I Miller, David Traver, John P Cooke, Wenbo Li, Leonard I Zon, Kaifu Chen, Yongping Bai, Longhou Fang
Hypercholesterolemia, the driving force of atherosclerosis, accelerates the expansion and mobilization of hematopoietic stem and progenitor cells (HSPCs). The molecular determinants connecting hypercholesterolemia with hematopoiesis are unclear. Here we report that a somite-derived pro-hematopoietic cue, AIBP, orchestrates HSPC emergence from the hemogenic endothelium, a type of specialized endothelium manifesting hematopoietic potential. Mechanistically, AIBP-mediated cholesterol efflux activates endothelial Srebp2, the master transcription factor for cholesterol biosynthesis, which in turn transactivates Notch and promotes HSPC emergence...
January 31, 2019: Science
Simona Daniele, Chiara Giacomelli, Deborah Pietrobono, Elisabetta Barresi, Rebecca Piccarducci, Valeria La Pietra, Sabrina Taliani, Federico Da Settimo, Luciana Marinelli, Ettore Novellino, Claudia Martini, Maria Letizia Trincavelli
The osteoblast generation from Mesenchymal stem cells (MSCs) is tightly coordinated by transcriptional networks and signalling pathways that control gene expression and protein stability of osteogenic "master transcription factors". Among these pathways, a great attention has been focused on p53 and its physiological negative regulator, the E3 ligase Murine double minute 2 (Mdm2). Nevertheless, the signalling that regulates Mdm2-p53 axis in osteoblasts remain to be elucidated, also considering that Mdm2 possesses numerous p53-independent activities and interacts with additional proteins...
January 28, 2019: Biochimica et biophysica acta. Molecular cell research
Rebeca Bailén, Ana María Pérez-Corral, Cristina Pascual, Mi Kwon, David Serrano, Jorge Gayoso, Pascual Balsalobre, Cristina Muñoz, José Luis Díez-Martín, Javier Anguita
BACKGROUND: Poor mobilization results are unexpected after G-CSF-induced peripheral blood stem cell collection in healthy donors. However, 2%-5% of the donors are poor mobilizers. Factors predicting CD34+-cell yield after mobilization in related alternative donors are still poorly known. PATIENTS AND METHODS: Baseline characteristics and efficacy results of G-CSF induced mobilization of 159 adult healthy donors in our institution from 2008 to 2016 were retrospectively analyzed...
January 29, 2019: Journal of Clinical Apheresis
Shang-Hsien Yang, Hsiang-Lin Wan, Ming-Hui Gu, Tso-Fu Wang
Polycythemia vera (PV) is relatively uncommon in early adulthood, and evidence about the prevalence of the Janus kinase 2 (JAK2) V617F mutation in the general population is limited. Here, we report a previously healthy volunteer peripheral blood stem cell (PBSC) donor who developed symptomatic PV with the JAK2 V617F mutation 2 years after PBSC mobilization and harvest. The characteristic mutation was identified retrospectively in the blood sample of the donor at the confirmation typing stage, which was before granulocyte colony-stimulating factor injection...
January 2019: Ci Ji Yi Xue za Zhi, Tzu-chi Medical Journal
Carmen Salvador-Palomeque, Francisco J Sanchez-Luque, Patrick R J Fortuna, Adam D Ewing, Ernst J Wolvetang, Sandra R Richardson, Geoffrey J Faulkner
The retrotransposon LINE-1 (L1) is a significant source of endogenous mutagenesis in humans. In each individual genome, a handful of retrotransposition-competent L1s (RC-L1s) can generate new heritable L1 insertions in the early embryo, primordial germline, and in germ cells. L1 retrotransposition can also occur in the neuronal lineage and cause somatic mosaicism. Although DNA methylation mediates L1 promoter repression, the temporal pattern of methylation applied to individual RC-L1s during neurogenesis is unclear...
January 28, 2019: Molecular and Cellular Biology
Qi Wen, Yuan Kong, Hong-Yan Zhao, Yuan-Yuan Zhang, Ting-Ting Han, Yu Wang, Lan-Ping Xu, Xiao-Hui Zhang, Xiao-Jun Huang
Macrophages (MΦs) are an important immune cell population that are essential for tissue homeostasis and disease pathogenesis. MΦs are now classified as either M1, which produce pro-inflammatory cytokines, or M2, which produce antiinflammatory cytokines. The impact of granulocyte colony-stimulating factor (G-CSF) on MΦs in humans is unclear. Moreover, little is known about the association between MΦ subsets in allografts and the occurrence of acute graft-versus-host disease (aGVHD) in patients who undergo allogeneic hematopoietic stem cell transplantation (allo-HSCT)...
January 25, 2019: Bone Marrow Transplantation
Seong Gyu Kwon, Insoo Park, Yang Woo Kwon, Tae Wook Lee, Gyu Tae Park, Jae Ho Kim
Stem cell mobilization plays important roles in the treatment of severe ischemic diseases, including myocardial infarction, limb ischemia, ischemic stroke, and acute kidney injury. Stem cell mobilization refers to the egress of heterogeneous stem cells residing in the bone marrow into the peripheral blood. In the clinic, granulocyte colony-stimulating factor (G-CSF) is the drug most commonly used to induce stem cell mobilization. Plerixafor, a direct antagonist of CXCR4, is also frequently used alone or in combination with G-CSF to mobilize stem cells...
January 24, 2019: Archives of Pharmacal Research
Satyam Arora, Navneet S Majhail, Hien Liu
Successful stem cell mobilization and adequate harvesting of hematopoietic progenitor cells (HPCs) is necessary for patients with multiple myeloma (MM) undergoing high-dose chemotherapy and autologous stem cell transplantation (ASCT). Several advances have increased the efficiency and yield of HPC collection methods, and sufficient CD34+ cell collection can be expected for most patients with MM considered to be ASCT candidates. However, in some patients, HPCs will fail to mobilize and an adequate number of CD34+ cells will not be collected...
December 20, 2018: Clinical Lymphoma, Myeloma & Leukemia
Xuelian Chen, Fang Cheng, Yanfang Liu, Lirong Zhang, Lian Song, Xiaojie Cai, Tao You, Xin Fan, Dongqing Wang, Aihua Gong, Haitao Zhu
BACKGROUND: High-mobility group box 1 (HMGB1), a common extracellular damage associated molecular pattern molecule, is overexpressed in several solid tumors including pancreatic carcinoma. We previously observed that radiotherapy induced dying cells secrete HMGB1 and accelerate pancreatic carcinoma progression through an unclear mechanism. METHODS: Using the Millicell system as an in vitro co-culture model, we performed quantitative reverse transcriptase-polymerase chain reaction, western blot and sphere forming ability analyses to access the effect of dying-cell-derived HMGB1 on CD133+ cancer cell stemness in vitro and in vivo...
January 21, 2019: EBioMedicine
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