bioimformatics

BACKGROUND: MicroRNAs (miRNAs) are a group of small noncoding RNAs that take part in diverse biological processes by suppressing target gene expression. Relatively few miRNAs have been studied in detail, especially miR-503, and hence the biological relevance of majority remains to be uncovered. Whether altered expression of miRNA-503 affects the immunity response to radiotherapy has yet to be addressed. RESULTS: In the present study, we applied ionizing radiation with a dose of either 0...

A 1 611 bp length complete coding sequence of the catalase (Cat) was obtained through bioimformatical analysis of the database of D.radiodurans genome, and then was amplified from D. radiodurans genomic DNA by polymerase chain reaction. The amplified gene was cloned into pKK223-3 vector and transformed into E.coli UM2, a Cat-deficient mutant. Staining of non-denaturing polyacrylamide gels for Cat activity demonstrated that pKK223-3 insert encoded a Cat that co-migrated with the CatB found in D.radiodurans cell lysates...