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CCN1 transport

Jin-Feng Zhao, Hsiang-Ying Chen, Jeng Wei, Shr-Jeng Jim Leu, Tzong-Shyuan Lee
AIM: CCN family member 1 (CCN1) is an extracellular matrix cytokine and appears in atherosclerotic lesions. However, we have no evidence to support the role of CCN1 in regulating of cholesterol metabolism and atherosclerosis. METHODS: Apolipoprotein E-deficient (apoE-/- ) mice were used as in vivo model. Oxidized low-density lipoprotein (oxLDL)-induced macrophage-foam cells were used as in vitro model. RT-PCR and western blot analysis was used for evaluating gene and protein expression, respectively...
October 22, 2018: Acta Physiologica
Genny Degani, Enrico Ragni, Pedro Botias, Davide Ravasio, Julia Calderon, Elena Pianezzola, Jose Manuel Rodriguez-Peña, Maria Antonietta Vanoni, Javier Arroyo, William A Fonzi, Laura Popolo
BACKGROUND: The cell wall is essential for the yeast to hypha (Y-H) transition that enables Candida albicans to invade human tissues and evade the immune system. The main constituent, β(1,3)-glucan, is remodeled by glucanosyltransferases of the GH72 family. Phr1p is responsible of glucan remodeling at neutral-alkaline pH and is essential for morphogenesis and virulence. Due to the pH-regulated expression of PHR1, the phr1Δ phenotype is manifested at pH > 6 and its severity increases with the rise in pH...
July 2, 2016: BMC Genomics
Bor-Chyuan Su, Fan-E Mo
Cell proliferation from pre-existing cardiomyocytes is a major source of cells for normal mammalian myocardial renewal or for regeneration after myocardial injury. These proliferative cardiomyocytes may act differently from the postmitotic cardiomyocytes in a stressed heart. Extracellular matrix molecule CCN1 is produced to promote Fas ligand (FasL)-induced cardiomyocyte apoptosis in mice with stress-induced cardiac injury. We aimed to investigate the effect of CCN1 on the proliferative cardiomyocytes. We used rat embryonic cardiomyoblast H9c2 cells to study the cardiotoxicity of CCN1...
June 2014: Cellular Signalling
Audrey Guillon-Munos, Katerina Oikonomopoulou, Noémie Michel, Chistopher R Smith, Agnès Petit-Courty, Sylvie Canepa, Pascale Reverdiau, Nathalie Heuzé-Vourc'h, Eleftherios P Diamandis, Yves Courty
Kallikrein-related peptidases (KLKs) are an emerging group of secreted serine proteases involved in several physiological and pathological processes. We used a degradomic approach to identify potential substrates of KLK12. MDA-MB-231 cells were treated either with KLK12 or vehicle control, and the proteome of the overlying medium was analyzed by mass spectrometry. CCN1 (cyr61, ctgf, nov) was among the proteins released by the KLK12-treated cells, suggesting that KLK12 might be responsible for the shedding of this protein from the cell surface...
July 22, 2011: Journal of Biological Chemistry
Yoshiki Mukudai, Satoshi Kubota, Takanori Eguchi, Kumi Sumiyoshi, Danilo Janune, Seiji Kondo, Satoru Shintani, Masaharu Takigawa
CCN1, a member of the CCN family of proteins, plays important physiological or pathological roles in a variety of tissues. In the present study, we initially found a highly guanine-cytosine (GC)-rich region of approximately 200 bp near the 5'-end of the open reading frame, which was always truncated by amplification of the corresponding cDNA region through the conventional polymerase chain reaction. An RNA in vitro folding assay and selective ribonuclease digestion of the corresponding segment of the ccn1 mRNA confirmed the involvement of a stable secondary structure...
December 15, 2010: Journal of Cellular Biochemistry
Kei Higuchi, Hisashi Iizasa, Yoshimichi Sai, Satomi Horieya, Kyeong-Eun Lee, Masami Wada, Masayuki Deguchi, Tomohiro Nishimura, Tomohiko Wakayama, Atsushi Tamura, Sachiko Tsukita, Noriko Kose, Young-Sook Kang, Emi Nakashima
The syncytiotrophoblast, which regulates maternal-fetal transfer of drugs, consists of a single layer in humans, but two layers, i.e., SynI and SynII, in rodents. Polar distribution of transporters in the apical and basal plasma membranes of syncytiotrophoblast is important for placental function in terms of vectorial transport of substrates, but the mechanisms that control protein distribution in the syncytiotrophoblast remain unclear. We have previously established rat syncytiotrophoblast cell lines, TR-TBT 18d-1 and TR-TBT 18d-2, which retain characteristics of SynI and SynII, respectively...
2010: Biological & Pharmaceutical Bulletin
Amy Bishop, Rachel Lane, Richard Beniston, Bernardo Chapa-y-Lazo, Carl Smythe, Peter Sudbery
Polarized growth is a fundamental property of cell growth and development. It requires the delivery of post-Golgi secretory vesicles to the site of polarized growth. This process is mediated by Rab GTPases activated by their guanine exchange factors (GEFs). The human fungal pathogen, Candida albicans, can grow in a budded yeast form or in a highly polarized hyphal form, and thus provides a model to study this phenomenon. During hyphal, but not yeast growth, secretory vesicles accumulate in an apical body called a Spitzenkörper, which acts to focus delivery of the vesicles to the tip...
September 1, 2010: EMBO Journal
Mary Hanna, Haibo Liu, Jawaria Amir, Yi Sun, Stephan W Morris, M A Q Siddiqui, Lester F Lau, Brahim Chaqour
Smooth muscle-rich tissues respond to mechanical overload by an adaptive hypertrophic growth combined with activation of angiogenesis, which potentiates their mechanical overload-bearing capabilities. Neovascularization is associated with mechanical strain-dependent induction of angiogenic factors such as CCN1, an immediate-early gene-encoded matricellular molecule critical for vascular development and repair. Here we have demonstrated that mechanical strain-dependent induction of the CCN1 gene involves signaling cascades through RhoA-mediated actin remodeling and the p38 stress-activated protein kinase (SAPK)...
August 21, 2009: Journal of Biological Chemistry
Bernard Perbal, Monia Zuntini, Diana Zambelli, Massimo Serra, Marika Sciandra, Lara Cantiani, Enrico Lucarelli, Piero Picci, Katia Scotlandi
PURPOSE: Osteosarcoma, the most common bone tumor, lacks prognostic markers that could distinguish patients before therapy and drive treatment choices. We assessed the prognostic value of CCN1, CCN2, and CCN3 genes, involved in fundamental biological processes. EXPERIMENTAL DESIGN: Expression of CCN1, CCN2, and CCN3 was measured by quantitative PCR in 45 newly diagnosed osteosarcomas. Cancer-specific survival was estimated using the Kaplan-Meier method. Associations with osteoblastic differentiation and/or drug response genes were assessed in tumor cells using Spearman correlation and Fisher's exact tests...
February 1, 2008: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
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