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Hui Chen, Mingjin Zou, Da Teng, Yu Hu, Jianmin Zhang, Wei He
γδ T cells function as sentinels in early host responses to infections and malignancies. Specifically, γδ T cells recognize tumor-associated stress antigens via T-cell receptor (TCR) γδ and play important roles in the antitumor immune response. In this study, we characterized the pattern of the human TCR γδ complementary determinant region 3 (CDR3) repertoire in patients with lung carcinoma (LC) via high-throughput sequencing. The results showed that the diversity of CDR3δ was significantly reduced, and that of CDR3γ was unchanged in LC patients compared with healthy individuals; in addition, LC patients shared significantly more CDR3δ sequences with each other than healthy individuals...
March 2019: Cellular & Molecular Immunology
Chu Lin, Jun Zhang
Introducing chimeric antigen receptor into immune cells against malignancies has contributed to a revolutionary innovation in cancer immunotherapy. As an important type of adaptive immune cells, T cells first caught researchers' attention and became great success in chimeric antigen receptor-based immunotherapy. However, engineered T cells seem to hit their bottleneck when resistance of cancerous cells, less encouraging responses in solid tumors and unwanted toxicities to the host remain to be solved. Meanwhile, innate immune cells get to join the race...
February 15, 2019: Science China. Life Sciences
Kay Tanita, Akihiro Hoshino, Ken-Ichi Imadome, Takahiro Kamiya, Kento Inoue, Tsubasa Okano, Tzu-Wen Yeh, Masakatsu Yanagimachi, Akira Shiraishi, Masataka Ishimura, Tilmann Schober, Meino Rohlfs, Masatoshi Takagi, Kohsuke Imai, Hidetoshi Takada, Shouichi Ohga, Christoph Klein, Tomohiro Morio, Hirokazu Kanegane
Chronic active Epstein-Barr virus (EBV) infection (CAEBV) is an EBV-associated lymphoproliferative disease characterized by repeated or sustainable infectious mononucleosis (IM)-like symptoms. EBV is usually detected in B cells in patients who have IM or Burkitt's lymphoma and even in patients with X-linked lymphoproliferative syndrome, which is confirmed to have vulnerability to EBV infection. In contrast, EBV infects T cells (CD4+ T, CD8+ T, and γδT) or NK cells mono- or oligoclonally in CAEBV patients...
2019: Frontiers in Pediatrics
Dongli Cai, Yunhui Tang, Xiaoying Yao
A successful pregnancy is a complicated process that involves the precisely timed regulation of endocrine as well as immune system. Despite increasing knowledge about immunology in gestation, the studies of immune cells in endometrium and decidua are still fragmented. Dynamic data is lacking on the transition of pre-pregnancy endometrial lymphocytes to initial pregnancy states as well as to second/third-trimester status. Here, we determined the composition of Gamma delta (γδ) T cells in endometrium and decidua from women with normal pregnancy and unexplained spontaneous abortion...
February 2019: Journal of Reproductive Immunology
Arjan van Laarhoven, Sofiati Dian, Suzanne van Dorp, Feby Purnama, Valerie A C M Koeken, Emira Diandini, Fitria Utami, Resvi Livia, Lika Apriani, Edwin Ardiansyah, Rob Ter Horst, Mihai G Netea, Tri Hanggono Achmad, Philip C Hill, Rovina Ruslami, Bachti Alisjahbana, James E Ussher, Agnes Indrati, Ayesha Verrall, Ahmad Rizal Ganiem, Reinout van Crevel
Immunopathology contributes to high mortality in tuberculous meningitis (TBM) but little is known about the blood and cerebrospinal fluid (CSF) immune response. We prospectively characterised the immune response of 160 TBM suspects in an Indonesian cohort, including 67 HIV-negative probable or definite TBM cases. TBM patients presented with severe disease and 38% died in 6 months. Blood from TBM patients analysed by flow cytometry showed lower αβT and γδT cells, NK cells and MAIT cells compared to 26 pulmonary tuberculosis patients (2...
January 29, 2019: Scientific Reports
Anneke Wilharm, Yaara Tabib, Maria Nassar, Annika Reinhardt, Gabriel Mizraji, Inga Sandrock, Oded Heyman, Joana Barros-Martins, Yuval Aizenbud, Abed Khalaileh, Luba Eli-Berchoer, Eran Elinav, Asaf Wilensky, Reinhold Förster, Herve Bercovier, Immo Prinz, Avi-Hai Hovav
γδT cells are a major component of epithelial tissues and play a role in tissue homeostasis and host defense. γδT cells also reside in the gingiva, an oral tissue covered with specialized epithelium that continuously monitors the challenging dental biofilm. Whereas most research on intraepithelial γδT cells focuses on the skin and intestine epithelia, our knowledge on these cells in the gingiva is still incomplete. In this study, we demonstrate that even though the gingiva develops after birth, the majority of gingival γδT cells are fetal thymus-derived Vγ6+ cells, and to a lesser extent Vγ1+ and Vγ4+ cells...
January 28, 2019: Proceedings of the National Academy of Sciences of the United States of America
Shuifang Jin, Ronglin Jiang, Shu Lei, Liming Jin, Chen Zhu, Wen Feng, Yingying Shen
BACKGROUND/AIMS: The aim of present study was to assess the protective effects of Shenfu injection (SI) on the intestinal mucosa and its regulation on the mucosal immune responses in rats with sepsis. MATERIALS AND METHODS: Sprague-Dawley rats were randomly divided into the sham, model, low-dose SI (LSF), and high-dose SI (HSF) groups. Sham animals underwent laparotomy only, whereas sepsis was modeled by cecal ligation and puncture in the remaining groups. At 2 h post-surgery, the LSF and HSF groups were intraperitoneally administered 5 and 20 mL/kg SI, respectively, whereas other animals with saline...
January 22, 2019: Turkish Journal of Gastroenterology: the Official Journal of Turkish Society of Gastroenterology
Yuhao Liu, Haocheng Lu, Chao Zhang, Jiahui Hu, Danyan Xu
Pressure overload-induced cardiac hypertrophy and remodeling are not simply mechanical responses to overloaded stress. They also involve participation of various immune cells, especially T cells. In this review, we summarized recent advances in understanding the roles of T cells in this process and the possible mechanisms underlying T cells involvement and modulation. In this pathological process, αβT cells play an indispensable role, with contribution of NKT cells and γδT cells. Moreover, among the αβT cells, CD4+ T cells rather than CD8+ T cells are dominant in the process with different subsets exerting diverse influences...
January 11, 2019: Journal of Molecular and Cellular Cardiology
Xiao-Shan Wei, Xue-Bin Pei, Ya-Lan Liu, Xiu-Zhi Wu, Huan-Zhong Shi, Qiong Zhou
γδT cells are an important source of IL-17A and play an anti- or pro-tumor role depending on the surrounding microenvironment. The precise role of γδT cells in the development of malignant pleural effusions (MPE) remains unknown. Using flow cytometry, the distribution and differentiation of γδT cells in wild-type (WT) and IL-10-⁄- mice were analyzed. The influence of γδT cells on the formation of MPE was carefully elucidated by depleting γδT cells from WT, IL-10-⁄-, and IL-17a-⁄- mice. The distribution of γδT17 cells in human MPE and peripheral blood was also determined...
January 4, 2019: American Journal of Respiratory Cell and Molecular Biology
Qin Fan, Rong Tao, Hang Zhang, Hongyang Xie, Lin Lu, Ting Wang, Min Su, Jian Hu, Qi Zhang, Qiujing Chen, Yoichiro Iwakura, Weifeng Shen, Ruiyan Zhang, Xiaoxiang Yan
BACKGROUND: Macrophage-associated immune response plays an important role in myocardial ischemia/reperfusion (IR) injury. Dectin-1, expressed mainly on activated myeloid cells, is crucial for the regulation of immune homeostasis as a pattern recognition receptor. However, its effects and roles during the myocardial IR injury remain unknown. METHODS: Genetic ablation, antibody blockade, or Dectin-1 activation, along with the adoptive bone marrow transfer chimeric model, was used to determine the functional significance of Dectin-1 in myocardial IR injury...
January 29, 2019: Circulation
Chinmay Kumar Mantri, Ashley L St John
Mast cells (MCs) are immune sentinels but whether they also function as antigen-presenting cells (APCs) remains elusive. Using mouse models of MC-deficiency, we report MC-dependent recruitment and activation of multiple T cell subsets to the skin and draining lymph nodes (LNs) during dengue virus (DENV) infection. Newly-recruited and locally-proliferating γδT cells were the first responding T cell subset to MC-driven inflammation and their production of IFN-γ was MC-dependent. MC-γδ T cell conjugates were observed consistently in infected peripheral tissues, suggesting a new role for MCs as non-conventional APCs for γδT cells...
December 18, 2018: Journal of Clinical Investigation
Katharine J Goodall, Angela Nguyen, Aya Matsumoto, Julie R McMullen, Sidonia B Eckle, Patrick Bertolino, Lucy C Sullivan, Daniel M Andrews
Class Ib major histocompatibility complex (MHC) is an extended family of molecules, which demonstrate tissue-specific expression and presentation of monomorphic antigens. These characteristics tend to imbue class Ib MHC with unique functions. H2-Q10 is potentially one such molecule that is overexpressed in the liver but its immunological function is not known. We have previously shown that H2-Q10 is a ligand for the natural killer cell receptor Ly49C and now, using H2-Q10-deficient mice, we demonstrate that H2-Q10 can also stabilize the expression of Qa-1b...
December 9, 2018: Immunology and Cell Biology
Osamu Imataki, Shumpei Uchida, Shigeyuki Yokokura, Makiko Uemura, Norimitsu Kadowaki
Here we report on the treatment of a 38-year-old woman with primary cutaneous γδT-cell lymphoma, which is a rare subset of cutaneous T-cell lymphoma. She presented with multiple subtle subcutaneous nodules, which were not clearly observed on computed tomography scans or after biopsy. However, F-fluorodeoxyglucose positron emission tomography (F-FDG-PET) accurately detected small cutaneous lesions. She achieved a second complete remission, as demonstrated by F-FDG-PET performed after pralatrexate infusion.
February 2019: Clinical Nuclear Medicine
Ding-Hong Wu, Miao-Miao Zhang, Ning Li, Xiong Li, Quan-Wei Cai, Wan-Lin Yu, Li-Ping Liu, Wei Zhu, Chuan-Jian Lu
The IL-17-producing CD4+ T cell and γδT cells play critical roles in the pathogenesis of psoriasis (PS). PSORI-CM02 is a representative herbal formula for the treatment for PS in South China. It was confirmed to improve PS without obvious side effects in the clinic. Here we sought to clarify whether and how PSORI-CM02 regulates T cell differentiation and functions in IMQ-induced psoriasis-like BALB/c mouse model. Mice pre-treated 3 days with PSORI-CM02 significantly alleviated skin inflammation, as reduced in PASI score and classic psoriatic characteristics in pathological sections...
December 1, 2018: Biomedicine & Pharmacotherapy
Catarina Simões, Isabel Silva, Anabela Carvalho, Sandra Silva, Susana Santos, Gilberto Marques, André Ribeiro, Adriana Roque, José Carda, A B Sarmento-Ribeiro, Maria do Rosário Domingues, Letícia Ribeiro, Artur Paiva
BACKGROUND: Vδ1+ T cells, a subset of γδ T cells, are responsible for innate-like immune responses. Recently, an anti-tumor function mediated by MHC-unrestricted recognition of lipid and stress molecules, has also been described in these cells. This study aimed to quantify and phenotypically characterize circulating Vδ1+ T cells in B cell Chronic Lymphocytic Leukemia (CLL) and Monoclonal B cell lymphocytosis (MBL). METHODS: This study enrolled 58 individuals distributed in five groups: Binet B and C CLL (n = 9), Binet A CLL (n = 26), High count-MBL (n = 10), Low count-MBL (n = 5), and a control group (n = 8)...
October 17, 2018: Cytometry. Part B, Clinical Cytometry
Soichiro Ueda, Koichi Fukunaga, Takahisa Takihara, Yoshiki Shiraishi, Tsuyoshi Oguma, Tetsuya Shiomi, Yusuke Suzuki, Makoto Ishii, Koichi Sayama, Shizuko Kagawa, Hiroyuki Hirai, Kinya Nagata, Masataka Nakamura, Taku Miyasho, Tomoko Betsuyaku, Koichiro Asano
Chemoattractant receptor homologous with Th2 cells (CRTH2), a receptor for prostaglandin D2 is preferentially expressed on Th2 lymphocytes, group 2 innate lymphoid cells, eosinophils, and basophils, and elicits production of type2 cytokines including profibrotic IL-13. We supposed lack of CRTH2 might be protective role against fibrotic lung disease and examined bleomycin-induced lung inflammation and fibrosis model with CRTH2-deficient (CRTH2-/-) or wild-type BALB/c mice. Compared to the wild-type, CRTH2-/- mice treated with bleomycin exhibited significantly higher mortality, enhanced accumulation of inflammatory cells 14-21 days after bleomycin injection, reduced pulmonary compliance, and increased levels of collagen and total protein in the lungs...
October 16, 2018: American Journal of Respiratory Cell and Molecular Biology
E Oliver, C Pope, E Clarke, C Langton Hewer, A D Ogunniyi, J C Paton, T Mitchell, R Malley, A Finn
Pneumococcal infections cause a large global health burden, and the search for serotype-independent vaccines continues. Existing conjugate vaccines reduce nasopharyngeal colonization by target serotypes. Such mucosal effects of novel antigens may similarly be important. CD4+ Th17 cell-dependent, antibody-independent reductions in colonization and enhanced clearance have been described in mice. Here we describe the evaluation of T helper type 17 (Th17) cytokine responses to candidate pneumococcal protein vaccine antigens in human cell culture, using adenoidal and peripheral blood mononuclear cells...
October 16, 2018: Clinical and Experimental Immunology
Kotaro Matsumoto, Masanori Obana, Arisa Kobayashi, Miho Kihara, Go Shioi, Shigeru Miyagawa, Makiko Maeda, Yasushi Sakata, Hiroyuki Nakayama, Yoshiki Sawa, Yasushi Fujio
Aims: Accumulating evidence demonstrates that cardiomyocyte death contributes to the onset and progression of heart failure (HF) after myocardial injury. Recent studies revealed that immune/inflammatory reactions play important roles in cardiovascular diseases. However, it remains unclear whether immunosurveillance system, which eliminates cytopathic cells, including infected or malignant cancer cells, is involved in cardiomyocyte death, though cardiomyocytes are exposed to pathological stresses during post-infarct remodeling...
October 11, 2018: Cardiovascular Research
David Brea, Carrie Poon, Michelle Murphy, Gabrielle Lubitz, Costantino Iadecola, Josef Anrather
Stroke is a devastating disease with a strong inflammatory component. It has been shown that part of this response is mediated by IL17+ γδT cells. γδT cells constitute a lymphocyte population with innate features that mainly populates epithelial surfaces including skin, intestine, and airways. We have shown that in the context of stroke, T cells migrate from the small intestine to the meninges but whether they can migrate from other epithelial surfaces is still unknown. Because of its proximity, one possible source of stroke-associated IL17+ γδT cells could be the Nasal-Associated Lymphoid Tissue (NALT) from which T cells could migrate along olfactory nerve sheaths through the cribriform plate into the brain and/or meninges...
2018: PloS One
Marian A Fernandez, Uet Yu, Angela L Ferguson, Dongwei Wang, Elise Francis, Ben Roediger, Wolfgang Weninger, Laurence C Cantrill, Anthony L Cunningham, Stephen I Alexander, Cheryl A Jones
HSV is an important cause of brain infection. Virus entry is often through breeches in the skin. γδT cells play an immunoprotective role in mice after corneal, genital or footpad (subcutaneous) HSV infection. Here we report that the presence of γδT cells in murine skin is associated with increased severity of herpetic disease, reduced protective cytokine responses and increased viral spread from the skin to the sensory ganglia in the zosteriform model. γδT cell-deficient (TCR δ -/-) mice displayed significantly decreased herpetic lesion severity after flank HSV infection compared to WT C57BL/6 controls at both primary and secondary skin infection sites...
October 1, 2018: Infectious Disorders Drug Targets
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