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γδT tuberculosis

Arjan van Laarhoven, Sofiati Dian, Suzanne van Dorp, Feby Purnama, Valerie A C M Koeken, Emira Diandini, Fitria Utami, Resvi Livia, Lika Apriani, Edwin Ardiansyah, Rob Ter Horst, Mihai G Netea, Tri Hanggono Achmad, Philip C Hill, Rovina Ruslami, Bachti Alisjahbana, James E Ussher, Agnes Indrati, Ayesha Verrall, Ahmad Rizal Ganiem, Reinout van Crevel
Immunopathology contributes to high mortality in tuberculous meningitis (TBM) but little is known about the blood and cerebrospinal fluid (CSF) immune response. We prospectively characterised the immune response of 160 TBM suspects in an Indonesian cohort, including 67 HIV-negative probable or definite TBM cases. TBM patients presented with severe disease and 38% died in 6 months. Blood from TBM patients analysed by flow cytometry showed lower αβT and γδT cells, NK cells and MAIT cells compared to 26 pulmonary tuberculosis patients (2...
January 29, 2019: Scientific Reports
T E Kononova, O I Urazova, V V Novitskii, I E Esimova, E G Churina
The study of subpopulation structure of IFNγ-producing T cells in patients with pulmonary tuberculosis revealed a decrease in the number of CD3+ IFNγ+ cells against the background of significantly increased IFNγ secretion in vitro irrespective of the clinical form of the disease and drug sensitivity of M. tuberculosis, most strongly expressed in case of the disseminated tuberculosis. In patients with infiltrative drug-sensitive and drug-resistant pulmonary tuberculosis, increased number of Th1/Th17 lymphocytes (CD4+ IFNγ+ IL-17A+ ) and, conversely, decreased number of blood γδT cells was detected...
July 12, 2018: Bulletin of Experimental Biology and Medicine
Jie Tang, Ce Chen, Cheng Zha, Jian-Rong Chang, Qiang Fang, Zhao-Hua Wang, Bai-Qing Li
OBJECTIVE: To investigate the effects of low molecular peptide of Mycobacterium tuberculosis heat-resistant antigen (Mtb-HAg-10k) on the production of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in peripheral blood T cells and test the feasibility of differential diagnosis between pulmonary tuberculosis (PTB) and latent tuberculosis infection (LTBI) by assessing the number of Mtb-HAg-10k-stimulated IFN-γ-producing T cells. METHODS: Peripheral blood mononuclear cells (PBMCs) were separated from the peripheral blood of 10 healthy adults, 6 individuals with LTBI and 13 patients with PTB...
November 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Li Chen, Hui Xia, Hongtao Wang, Lingling Sheng, Zhiqing Xu, Yao Sun, Jie Tang, Baiqing Li
OBJECTIVE: To investigate the effects of Mycobacterium tuberculosis heat-resistant antigen (MTB-HAg) and anti-TCR γδ monoclonal antibody (mAb) on the induction and differentiation of human IL-17-producing γδT cells under polarization culture conditions. METHODS: From human peripheral blood mononuclear cells, the γδT cells were purified by magnetic-activated cell sorting (MACS) and then stimulated with MTB-HAg or anti-TCRγδ mAb and with or without anti-CD28 mAb, and cultured in the presence or absence of the cytokine cocktails (CK) (IL-1β, IL-6, TGF-β and IL-23) for 10 to 12 days...
February 2015: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Anyou Zhu, Hezuo Lv, Lunjun Zhang, Jianguo Hu, Fengchao Wang, Baiqing Li
OBJECTIVE: To investigate Th2 differentiation features of Mycobacterium tuberculosis heat resistant antigens (MTB-HAg)-activated human γδT cells and the regulation of transcription factor T-box expression in T cells (T-bet) and GATA-binding protein 3 (GATA-3) on differentiation. METHODS: Peripheral blood mononuclear cells (PBMCs) were stimulated with MTB-HAg to generate MTB-HAg-activated T cells (MTBAT) and expanded in the neutral condition or Th2 polarizing condition...
January 2015: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Alessandro Poggi, Maria Raffaella Zocchi
Among γδT cells, the Vδ1 subset, resident in epithelial tissues, is implied in the defense against viruses, fungi, and certain hematological malignancies, while the circulating Vδ2 subpopulation mainly respond to mycobacteria and solid tumors. Both subsets can be activated by stress-induced molecules (MIC-A, MIC-B, ULBPs) to produce pro-inflammatory cytokines and lytic enzymes and destroy bacteria or damaged cells. γδT lymphocytes can also recognize lipids, as those associated to M. tuberculosis, presented by the CD1 molecule, or phosphoantigens (P-Ag), either autologous, which accumulates in virus-infected cells, or microbial produced by prokaryotes and parasites...
2014: Frontiers in Immunology
Michael Olin, Keumhwa Choi, Thomas W Molitor
Microglia, the macrophages of the central nervous system (CNS), are both the principle target cells for Mycobacterium infection in the CNS and serve a critical role in defense of the brain. If microglia's functions are altered due to immunosuppressive agents such as opiates, perturbation in defense of the brain may occur, including defense against CNS Tuberculosis. This study was designed to determine if Mycobacterium infected microglia activate γδT lymphocytes and if the opiate morphine alters the capability of microglia to activate γδT lymphocytes...
December 2011: Journal of Neuroimmune Pharmacology: the Official Journal of the Society on NeuroImmune Pharmacology
Wei Yuan, Na Dong, Lifang Zhang, Jiangning Liu, Shuzhu Lin, Zhiguang Xiang, Hongwei Qiao, Wei Tong, Chuan Qin
Tuberculosis remains a major infectious disease worldwide due to the low efficacy of available vaccine of the Mycobacterium bovis Bacillus Calmette-Guérin (BCG). DNA vaccines are especially promising candidates; however, the efficacy of DNA vaccine expressing single antigen of Mycobacterium tuberculosis (MTb) is limited. In this study, a plasmid DNA vaccine, pAEH, was constructed and designed to express a fusion protein of the Ag85B, Esat6, and HspX of MTb. Its immunogenicity and protective efficacy as well as therapeutic effect were assessed in a mouse model of tuberculosis...
March 23, 2012: Vaccine
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